Rorug05G0553100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001279
Physical Location & Seq
Reverse (-)
74107062 .. 74108294
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0553100.1

Sequence Viewer

Length: 1233 bp
ATGGAGAAGGAGGAGCTGAACTTTGACCTCCCTGAAGATATAATTCTGCAGATTCTGTGTAGGTTGCCAGTCAAGTCTTTGATTCGGTTCAGTTGTGTATCGAAACGGTGGCATAGTATAATAATTTTTGACCCACAATTTGGAAAAGCCCACCTCAAACTAGCATCTGAGCTGAGAAACATCACTAAGAGACTCATTCTCTCCGTATCCCCTTATGTTCAACTACATACCAGAGTTAATCTACCCCTTCCAATTCAATCCTTAGAAGGGTTATTTGGAGTTAGTTCTTCGGTCACAAATCTCAACTTACCATCCGAGGAGCATTGCAGAATTAAAATACTGGCTTCGTGCAACGGGTTGGTAGTTCTAGGTAATTTTTACAAAGAGTGGTCTATTTGGAACCCATCAACTGGGTTCTTCCGCAAAATTCCTGCTCCAGACTTTTCATCAGTAACGACAAAATCTAGAACAATTCACGTAGATAAATATGGTTTTGGCTATGTCTCATCCACTGATGACTACAAACTTGTTTTTGTAGTCCACATTGGTTCTGATGACCCTGTTTCTGCACCGGCTGTTAAGATCTTCATATTCTCAGTGAGAGCTAACTGTTGGAAAATTAGTGAAGTTTCTCGCTGGCCATTCCTCGAGTACAGACCGACTAGTGACTGTGGAACTCTTTCAAATGAAGCGATCCATTGGGTTAACCGATACTATGACTATGACTATCTAGAAGACTACATTGAAGCAATATTTGACGTCTGTGCTTTTGATTTGGAAAATGAGGAGTTCCGTAAAGTGCCTGTTCCTTGTTTTAACCAAGATGATGATCGAGTTTTCGATAGAAGAATGCGGACTGTTGTTCATTCAGGAGGATGCCTTTGCGTTTTGTCTCAGACTATGCATAACAGGGGTGGGGACCGTGGGGATATTGAATTGTGGATGATGAGAGATTACGGGGTTTCCAATTCATGGGTGAAACTCTACAAATTAAGCAAGTATGATTTACCGGTTCCGTTTACAAAATCTTATGGTTGGCAATCTTGGGATCTAAGTTTGGTTACCGAAAGTGGTTCAATTGTGATCAAGTTTGACAACGAGTTGCTCAGGATTGAATGCCGTAAAGAAGAGAAGCCGGTTTGTGTCGGCCGATATAGGCCTGAGGAGGTGTCTGGGTGTTACTTTTTTAACGCGACTGGGTATGATGAAACTCTAATTTCTGTACCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

410

Amino Acids

47.32

Weight (kDa)

5.92

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 41 8.4e-12 F-box domain
F-box-like PF12937 9 - 41 3e-09 F-box-like
FBA_3 PF08268 98 - 361 1.7e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 762
AccB7I CCANNNNNTGG 3 cut(s) 140, 410, 972
AccII CGCG 1 cut(s) 1193
AciI CCGC 2 cut(s) 421, 853
AclWI GGATC 2 cut(s) 688, 1056
AcoI YGGCCR 2 cut(s) 638, 1147
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 759
AfaI GTAC 2 cut(s) 653, 1224
AfiI CCNNNNNNNGG 4 cut(s) 140, 267, 410, 972
AgeI ACCGGT 1 cut(s) 1009
AgsI TTSAA 7 cut(s) 221, 257, 684, 746, 935, 1077, 1115
AhlI ACTAGT 1 cut(s) 662
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 16, 172, 605
AluI AGCT 3 cut(s) 16, 172, 605
Alw26I GTCTC 3 cut(s) 184, 508, 897
AlwI GGATC 2 cut(s) 688, 1056
AlwNI CAGNNNCTG 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 3 cut(s) 638, 1147, 1157
ApoI RAATTY 1 cut(s) 426
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AsiGI ACCGGT 1 cut(s) 1009
Asp700I GAANNNNTTC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 919
AsuHPI GGTGA 1 cut(s) 988
AvaI CYCGRG 1 cut(s) 647
AvaII GGWCC 1 cut(s) 919
AxyI CCTNAGG 1 cut(s) 1161
BalI TGGCCA 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 741
BccI CCATC 2 cut(s) 319, 412
BceAI ACGGC 1 cut(s) 1104
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 1 cut(s) 1083
BcoDI GTCTC 3 cut(s) 184, 508, 897
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 5 cut(s) 161, 368, 465, 663, 731
BfmI CTRYAG 1 cut(s) 47
BfuI GTATCC 1 cut(s) 217
BglII AGATCT 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 919
BmeT110I CYCGRG 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 919
BmiI GGNNCC 3 cut(s) 401, 920, 1014
BmrI ACTGGG 2 cut(s) 420, 1206
BmsI GCATC 2 cut(s) 173, 866
BmuI ACTGGG 2 cut(s) 420, 1206
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 1106
BsaAI YACGTR 1 cut(s) 478
BsaBI GATNNNNATC 1 cut(s) 828
BsaHI GRCGYC 1 cut(s) 759
BsaJI CCNNGG 2 cut(s) 315, 922
BsaWI WCCGGW 1 cut(s) 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 267, 410, 972
Bse118I RCCGGY 3 cut(s) 571, 1009, 1135
Bse1I ACTGG 4 cut(s) 68, 345, 415, 1201
Bse21I CCTNAGG 1 cut(s) 1161
Bse3DI GCAATG 1 cut(s) 322
Bse8I GATNNNNATC 1 cut(s) 828
BseDI CCNNGG 2 cut(s) 315, 922
BseGI GGATG 4 cut(s) 311, 506, 881, 948
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 4 cut(s) 140, 267, 410, 972
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 6 cut(s) 159, 164, 609, 908, 1120, 1152
BseNI ACTGG 4 cut(s) 68, 345, 415, 1201
BseRI GAGGAG 4 cut(s) 26, 332, 800, 1178
BseX3I CGGCCG 1 cut(s) 1147
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 1193
Bsh1285I CGRYCG 1 cut(s) 1150
BshFI GGCC 3 cut(s) 640, 1149, 1159
BshTI ACCGGT 1 cut(s) 1009
BsiEI CGRYCG 1 cut(s) 1150
BsiHKCI CYCGRG 1 cut(s) 647
BsiSI CCGG 3 cut(s) 572, 1010, 1136
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 4 cut(s) 140, 267, 410, 972
BsmAI GTCTC 3 cut(s) 184, 508, 897
BsmFI GGGAC 1 cut(s) 932
BsmI GAATGC 2 cut(s) 855, 1121
BsnI GGCC 3 cut(s) 640, 1149, 1159
BsoBI CYCGRG 1 cut(s) 647
Bsp143I GATC 5 cut(s) 582, 693, 829, 1048, 1083
BspACI CCGC 2 cut(s) 421, 853
BspANI GGCC 3 cut(s) 640, 1149, 1159
BspCNI CTCAG 6 cut(s) 160, 165, 608, 907, 1119, 1153
BspFNI CGCG 1 cut(s) 1193
BspLI GGNNCC 3 cut(s) 401, 920, 1014
BspMAI CTGCAG 1 cut(s) 51
BspPI GGATC 2 cut(s) 688, 1056
BsrDI GCAATG 1 cut(s) 322
BsrFI RCCGGY 3 cut(s) 571, 1009, 1135
BsrI ACTGG 4 cut(s) 68, 345, 415, 1201
BssAI RCCGGY 3 cut(s) 571, 1009, 1135
BssECI CCNNGG 2 cut(s) 315, 922
BssMI GATC 5 cut(s) 582, 693, 829, 1048, 1083
BssNI GRCGYC 1 cut(s) 759
Bst4CI ACNGT 5 cut(s) 108, 611, 671, 859, 923
Bst6I CTCTTC 1 cut(s) 1122
BstACI GRCGYC 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 638
BstDEI CTNAG 9 cut(s) 168, 173, 186, 262, 595, 894, 1052, 1106, 1161
BstDSI CCRYGG 1 cut(s) 922
BstEII GGTNACC 1 cut(s) 1060
BstF5I GGATG 4 cut(s) 311, 506, 881, 948
BstFNI CGCG 1 cut(s) 1193
BstKTI GATC 5 cut(s) 585, 696, 832, 1051, 1086
BstMAI GTCTC 3 cut(s) 184, 508, 897
BstMBI GATC 5 cut(s) 582, 693, 829, 1048, 1083
BstMCI CGRYCG 1 cut(s) 1150
BstPI GGTNACC 1 cut(s) 1060
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 1 cut(s) 1193
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 582, 1048
BstYI RGATCY 2 cut(s) 582, 1048
BstZI CGGCCG 1 cut(s) 1147
Bsu36I CCTNAGG 1 cut(s) 1161
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 3 cut(s) 640, 1149, 1159
BtgI CCRYGG 1 cut(s) 922
BtsCI GGATG 4 cut(s) 311, 506, 881, 948
BtsIMutI CAGTG 2 cut(s) 510, 603
Cac8I GCNNGC 1 cut(s) 638
CaiI CAGNNNCTG 1 cut(s) 55
Cfr10I RCCGGY 3 cut(s) 571, 1009, 1135
Cfr13I GGNCC 1 cut(s) 919
Csp6I GTAC 2 cut(s) 652, 1223
CspAI ACCGGT 1 cut(s) 1009
CviAII CATG 1 cut(s) 972
CviQI GTAC 2 cut(s) 652, 1223
DdeI CTNAG 9 cut(s) 168, 173, 186, 262, 595, 894, 1052, 1106, 1161
DpnI GATC 5 cut(s) 584, 695, 831, 1050, 1085
DpnII GATC 5 cut(s) 582, 693, 829, 1048, 1083
EaeI YGGCCR 2 cut(s) 638, 1147
EagI CGGCCG 1 cut(s) 1147
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
EclXI CGGCCG 1 cut(s) 1147
Eco147I AGGCCT 1 cut(s) 1159
Eco47I GGWCC 1 cut(s) 919
Eco52I CGGCCG 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1161
Eco88I CYCGRG 1 cut(s) 647
Eco91I GGTNACC 1 cut(s) 1060
EcoO65I GGTNACC 1 cut(s) 1060
EcoT22I ATGCAT 1 cut(s) 906
FaeI CATG 1 cut(s) 975
FaqI GGGAC 1 cut(s) 932
FatI CATG 1 cut(s) 971
FbaI TGATCA 1 cut(s) 1083
FokI GGATG 4 cut(s) 298, 493, 888, 955
FspBI CTAG 5 cut(s) 161, 368, 465, 663, 731
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 3 cut(s) 640, 1149, 1159
HapII CCGG 3 cut(s) 572, 1010, 1136
Hin1I GRCGYC 1 cut(s) 759
Hin1II CATG 1 cut(s) 975
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 3 cut(s) 52, 82, 192
HpaI GTTAAC 1 cut(s) 706
HpaII CCGG 3 cut(s) 572, 1010, 1136
HphI GGTGA 1 cut(s) 988
Hpy166II GTNNAC 3 cut(s) 541, 706, 1020
Hpy188I TCNGA 4 cut(s) 169, 316, 553, 897
Hpy188III TCNNGA 5 cut(s) 437, 465, 731, 870, 1108
Hpy8I GTNNAC 3 cut(s) 541, 706, 1020
HpyAV CCTTC 2 cut(s) 257, 260
HpyCH4III ACNGT 5 cut(s) 108, 611, 671, 859, 923
HpyCH4IV ACGT 2 cut(s) 477, 759
HpyCH4V TGCA 5 cut(s) 49, 327, 351, 569, 904
HpyF3I CTNAG 9 cut(s) 168, 173, 186, 262, 595, 894, 1052, 1106, 1161
HpySE526I ACGT 2 cut(s) 477, 759
Hsp92I GRCGYC 1 cut(s) 759
Hsp92II CATG 1 cut(s) 975
Ksp22I TGATCA 1 cut(s) 1083
KspAI GTTAAC 1 cut(s) 706
Kzo9I GATC 5 cut(s) 582, 693, 829, 1048, 1083
LmnI GCTCC 3 cut(s) 13, 319, 439
LweI GCATC 2 cut(s) 173, 866
MaeI CTAG 5 cut(s) 161, 368, 465, 663, 731
MaeII ACGT 2 cut(s) 477, 759
MaeIII GTNAC 5 cut(s) 292, 451, 665, 1060, 1178
MalI GATC 5 cut(s) 584, 695, 831, 1050, 1085
MboI GATC 5 cut(s) 582, 693, 829, 1048, 1083
MboII GAAGA 7 cut(s) 47, 279, 409, 577, 746, 858, 1139
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 2 cut(s) 582, 1048
MlsI TGGCCA 1 cut(s) 640
MluNI TGGCCA 1 cut(s) 640
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 593
MnlI CCTC 9 cut(s) 4, 38, 164, 310, 656, 778, 866, 1156, 1159
Mox20I TGGCCA 1 cut(s) 640
Mph1103I ATGCAT 1 cut(s) 906
MroXI GAANNNNTTC 1 cut(s) 679
MscI TGGCCA 1 cut(s) 640
MseI TTAA 7 cut(s) 237, 333, 579, 705, 816, 992, 1188
Msp20I TGGCCA 1 cut(s) 640
MspI CCGG 3 cut(s) 572, 1010, 1136
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 2 cut(s) 855, 1121
MvnI CGCG 1 cut(s) 1193
NdeII GATC 5 cut(s) 582, 693, 829, 1048, 1083
NlaIII CATG 1 cut(s) 975
NlaIV GGNNCC 3 cut(s) 401, 920, 1014
NmuCI GTSAC 2 cut(s) 292, 665
NsiI ATGCAT 1 cut(s) 906
PaeR7I CTCGAG 1 cut(s) 647
PceI AGGCCT 1 cut(s) 1159
PctI GAATGC 2 cut(s) 855, 1121
PdmI GAANNNNTTC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 52, 82
PflMI CCANNNNNTGG 3 cut(s) 140, 410, 972
PinAI ACCGGT 1 cut(s) 1009
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
Ppu21I YACGTR 1 cut(s) 478
PspEI GGTNACC 1 cut(s) 1060
PspN4I GGNNCC 3 cut(s) 401, 920, 1014
PspPI GGNCC 1 cut(s) 919
PspXI VCTCGAGB 1 cut(s) 647
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 582, 1048
RsaI GTAC 2 cut(s) 653, 1224
RsaNI GTAC 2 cut(s) 652, 1223
SaqAI TTAA 7 cut(s) 237, 333, 579, 705, 816, 992, 1188
Sau3AI GATC 5 cut(s) 582, 693, 829, 1048, 1083
Sau96I GGNCC 1 cut(s) 919
SchI GAGTC 1 cut(s) 186
SfaNI GCATC 2 cut(s) 173, 866
SfcI CTRYAG 1 cut(s) 47
Sfr274I CTCGAG 1 cut(s) 647
SinI GGWCC 1 cut(s) 919
SlaI CTCGAG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
SpeI ACTAGT 1 cut(s) 662
SseBI AGGCCT 1 cut(s) 1159
SsiI CCGC 2 cut(s) 421, 853
SspI AATATT 1 cut(s) 753
SspMI CTAG 5 cut(s) 161, 368, 465, 663, 731
StuI AGGCCT 1 cut(s) 1159
TaaI ACNGT 5 cut(s) 108, 611, 671, 859, 923
TaiI ACGT 2 cut(s) 480, 762
TaqI TCGA 4 cut(s) 101, 648, 832, 840
TaqII GACCGA 2 cut(s) 280, 673
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 2 cut(s) 52, 82
Tru1I TTAA 7 cut(s) 237, 333, 579, 705, 816, 992, 1188
Tru9I TTAA 7 cut(s) 237, 333, 579, 705, 816, 992, 1188
TscAI CASTG 2 cut(s) 517, 603
TseFI GTSAC 2 cut(s) 292, 665
Tsp45I GTSAC 2 cut(s) 292, 665
TspDTI ATGAA 6 cut(s) 435, 577, 702, 854, 960, 1221
TspGWI ACGGA 3 cut(s) 193, 782, 1005
TspRI CASTG 2 cut(s) 517, 603
Van91I CCANNNNNTGG 3 cut(s) 140, 410, 972
VpaK11BI GGWCC 1 cut(s) 919
XapI RAATTY 1 cut(s) 426
XbaI TCTAGA 2 cut(s) 464, 730
XhoI CTCGAG 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 679
XspI CTAG 5 cut(s) 161, 368, 465, 663, 731
ZraI GACGTC 1 cut(s) 760
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.