pycom10g08580

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001279
Physical Location & Seq
Forward (+)
11311760 .. 11313950
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08580.1

Sequence Viewer

Length: 411 bp
ATGGCTTGGCGCTCAGCTGGTTCGCTTTCTCGATCATTGGTGTCCACCGCTAGGGTTTCGTCGCTTCGCTTGGCGGCCCCTCTCCGTCGCCTCCGCCCGCCGTCTCTCTCTACTCCTCGCCGCCTCTTTGCTTCTCCCAGGAACGTAGGAGAGCTGGGATGCATACAATCGCTTATGCCAATGCACAACGTGACGGCTGCAACCTGCCTCACATCTCACCTGGCTGCCAACGGGCAGGGATGTTGCGAGTTGTCATACGGTACCTTCTGCCGTACTTGTGAGGAGGAATGGAAAAGATGGGTGATGCGCAAGGACTTCCAATCATCTAAAGGCAGGAGAATGATCAATTGTCGTAGGTCCTCTTTCATTCCCTTTTTTGGACATTATAGATATTTAACTTTTCAATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.45

Weight (kDa)

11.0

Isoelectric Point (pI)

83.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AciI CCGC 5 cut(s) 48, 74, 94, 98, 121
AdeI CACNNNGTG 1 cut(s) 190
AfaI GTAC 2 cut(s) 262, 274
AfiI CCNNNNNNNGG 2 cut(s) 51, 377
AgsI TTSAA 1 cut(s) 404
AjnI CCWGG 2 cut(s) 137, 219
AluBI AGCT 2 cut(s) 17, 154
AluI AGCT 2 cut(s) 17, 154
Alw26I GTCTC 1 cut(s) 108
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 2 cut(s) 197, 224
Asp718I GGTACC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 12, 309
AspS9I GGNCC 2 cut(s) 76, 357
AsuHPI GGTGA 2 cut(s) 209, 313
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 184, 211
BccI CCATC 1 cut(s) 291
BceAI ACGGC 3 cut(s) 85, 210, 255
BciT130I CCWGG 2 cut(s) 139, 221
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 13
BfuAI ACCTGC 1 cut(s) 212
BisI GCNGC 4 cut(s) 75, 121, 198, 225
BlpI GCTNAGC 1 cut(s) 13
BlsI GCNGC 4 cut(s) 76, 122, 199, 226
Bme1390I CCNGG 2 cut(s) 139, 221
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 76, 357
BmiI GGNNCC 2 cut(s) 78, 262
BmrFI CCNGG 2 cut(s) 139, 221
BmsI GCATC 2 cut(s) 149, 294
Bpu1102I GCTNAGC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 377
BseBI CCWGG 2 cut(s) 139, 221
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 2 cut(s) 164, 245
BseLI CCNNNNNNNGG 2 cut(s) 51, 377
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 2 cut(s) 105, 296
BseXI GCAGC 2 cut(s) 184, 211
BseYI CCCAGC 1 cut(s) 154
BshFI GGCC 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 51, 377
BsmAI GTCTC 1 cut(s) 108
BsmBI CGTCTC 1 cut(s) 108
BsnI GGCC 1 cut(s) 77
Bsp143I GATC 2 cut(s) 32, 342
Bsp1720I GCTNAGC 1 cut(s) 13
BspACI CCGC 5 cut(s) 48, 74, 94, 98, 121
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 2 cut(s) 78, 262
BspMI ACCTGC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 2 cut(s) 32, 342
Bst2UI CCWGG 2 cut(s) 139, 221
Bst4CI ACNGT 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 13
BstF5I GGATG 2 cut(s) 164, 245
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 2 cut(s) 12, 309
BstKTI GATC 2 cut(s) 35, 345
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 32, 342
BstNI CCWGG 2 cut(s) 139, 221
BstSCI CCNGG 2 cut(s) 137, 219
BstV1I GCAGC 2 cut(s) 184, 211
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 2 cut(s) 164, 245
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 1 cut(s) 98
CfoI GCGC 2 cut(s) 12, 309
Cfr13I GGNCC 2 cut(s) 76, 357
Csp6I GTAC 2 cut(s) 261, 273
CviJI RGCY 6 cut(s) 5, 17, 77, 154, 197, 224
CviKI_1 RGCY 6 cut(s) 5, 17, 77, 154, 197, 224
CviQI GTAC 2 cut(s) 261, 273
DdeI CTNAG 1 cut(s) 13
DpnI GATC 2 cut(s) 34, 344
DpnII GATC 2 cut(s) 32, 342
DraIII CACNNNGTG 1 cut(s) 190
EciI GGCGGA 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 137, 219
EcoT22I ATGCAT 1 cut(s) 164
Esp3I CGTCTC 1 cut(s) 108
FaiI YATR 4 cut(s) 164, 176, 256, 387
FauI CCCGC 1 cut(s) 105
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 75, 121, 198, 225
FokI GGATG 2 cut(s) 171, 252
Fsp4HI GCNGC 4 cut(s) 75, 121, 198, 225
FspBI CTAG 1 cut(s) 51
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 2 cut(s) 11, 308
GluI GCNGC 4 cut(s) 75, 121, 198, 225
GsaI CCCAGC 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 1 cut(s) 77
HhaI GCGC 2 cut(s) 12, 309
Hin6I GCGC 2 cut(s) 10, 307
HinP1I GCGC 2 cut(s) 10, 307
HphI GGTGA 2 cut(s) 209, 313
Hpy166II GTNNAC 1 cut(s) 45
Hpy188III TCNNGA 2 cut(s) 30, 408
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 2 cut(s) 64, 90
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 2 cut(s) 144, 189
HpyCH4V TGCA 3 cut(s) 162, 184, 200
HpyF3I CTNAG 1 cut(s) 13
HpySE526I ACGT 2 cut(s) 144, 189
HspAI GCGC 2 cut(s) 10, 307
KpnI GGTACC 1 cut(s) 264
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 2 cut(s) 32, 342
LpnPI CCDG 9 cut(s) 3, 124, 140, 151, 206, 217, 221, 233, 319
Lsp1109I GCAGC 2 cut(s) 184, 211
LweI GCATC 2 cut(s) 149, 294
MaeI CTAG 1 cut(s) 51
MaeII ACGT 2 cut(s) 144, 189
MaeIII GTNAC 1 cut(s) 190
MalI GATC 2 cut(s) 34, 344
MboI GATC 2 cut(s) 32, 342
MfeI CAATTG 1 cut(s) 346
MluCI AATT 1 cut(s) 346
MnlI CCTC 8 cut(s) 90, 101, 126, 134, 218, 274, 277, 370
Mph1103I ATGCAT 1 cut(s) 164
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 139, 221
MunI CAATTG 1 cut(s) 346
MvaI CCWGG 2 cut(s) 139, 221
NdeII GATC 2 cut(s) 32, 342
NlaIV GGNNCC 2 cut(s) 78, 262
NmuCI GTSAC 1 cut(s) 190
NsbI TGCGCA 1 cut(s) 308
NsiI ATGCAT 1 cut(s) 164
PkrI GCNGC 4 cut(s) 76, 122, 199, 226
PpuMI RGGWCCY 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 137, 219
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 2 cut(s) 137, 219
PspN4I GGNNCC 2 cut(s) 78, 262
PspPI GGNCC 2 cut(s) 76, 357
PspPPI RGGWCCY 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 262, 274
RsaNI GTAC 2 cut(s) 261, 273
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 4 cut(s) 75, 121, 198, 225
Sau3AI GATC 2 cut(s) 32, 342
Sau96I GGNCC 2 cut(s) 76, 357
ScrFI CCNGG 2 cut(s) 139, 221
SetI ASST 8 cut(s) 19, 147, 156, 192, 206, 222, 266, 359
SfaNI GCATC 2 cut(s) 149, 294
SinI GGWCC 1 cut(s) 357
Sse9I AATT 1 cut(s) 346
SsiI CCGC 5 cut(s) 48, 74, 94, 98, 121
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 137, 219
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 2 cut(s) 147, 192
TaqI TCGA 1 cut(s) 31
TasI AATT 1 cut(s) 346
TauI GCSGC 2 cut(s) 77, 123
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 197, 224
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 355
TspGWI ACGGA 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 357
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.