Rorug05G0553000

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001279
Physical Location & Seq
Reverse (-)
74089047 .. 74089742
696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0553000.1

Sequence Viewer

Length: 696 bp
ATGTCAATGTCGAAGATGAAAAATGAGAGCTTTGATCTACATGAAGATGTTATAGTGAAGATTCTTTGCCGGTTGCCGGTCAAGTCCTTGATCCGGTTCACTTGTGTGTCGAAACGGTGGCGTTCTATTATCATTTCTGATCCTCAATTTGGCAAGTCTCACCTTCAAGTAGCATCACAGCAGGGAACCCTCTGTCCAAAAGTCCTCATCTCCATCTACCCTACAATTAAAGCCATACAAGGTCAAATACCCTATCCTTTTAAAGATGACACGCCTTGGTTACCCTCTCGATTTCAATCCTTGGAAGGTCATTCGTCAGTCAATAATCTCACCTTCCCATCGGAGGAGAAGAGCCAACGAGTAATGGCCTCGTGCAATGGTTTGGTACTTCTAGGTGAATACTATAAAAGTTACTTTAAGAACTTGTCTATCTGGAATCCATCAACTGGATTTTTTCGCAAAATACCTAGTCCAAGTTTTGGGTTTGAGGTGACAAAATCAACAGAAAAGAAAAAGTACAGAAGCTTTTTATATTATGGTTTTGGTCAAGTGTCAGCCACTGACGACTACAAGCTTGTTTTCATAAAACCCGCCCTTGGTGATTTTGTGGATGTTCATGTCTTCTCACTCAGAGCCAACCTTTGGAAAGTTATTAAAGCTCCTTCCTCTTCATGCTGTGGCTGGATTGACGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

26.28

Weight (kDa)

9.45

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 47 8.3e-10 F-box domain
F-box-like PF12937 15 - 47 4e-06 F-box-like
FBA_3 PF08268 97 - 221 6.8e-08 F-box associated beta propeller domain
FBA_1 PF07734 101 - 222 1.6e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 446, 479, 642
AciI CCGC 1 cut(s) 591
AclWI GGATC 2 cut(s) 85, 134
AfaI GTAC 2 cut(s) 387, 518
AfiI CCNNNNNNNGG 8 cut(s) 76, 93, 149, 343, 446, 479, 596, 642
AgsI TTSAA 2 cut(s) 167, 296
AleI CACNNNNGTG 1 cut(s) 104
AloI GAACNNNNNNTCC 2 cut(s) 178, 210
AluBI AGCT 4 cut(s) 30, 525, 574, 659
AluI AGCT 4 cut(s) 30, 525, 574, 659
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 2 cut(s) 85, 134
AlwNI CAGNNNCTG 1 cut(s) 560
AoxI GGCC 1 cut(s) 366
AsuHPI GGTGA 5 cut(s) 152, 322, 407, 502, 611
BauI CACGAG 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 613
BccI CCATC 3 cut(s) 221, 346, 448
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 2 cut(s) 392, 468
BmiI GGNNCC 1 cut(s) 187
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 613
BsaBI GATNNNNATC 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 275, 300, 595
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 8 cut(s) 76, 93, 149, 343, 446, 479, 596, 642
Bse118I RCCGGY 2 cut(s) 69, 76
Bse1I ACTGG 1 cut(s) 451
Bse3DI GCAATG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 275, 300, 595
BseGI GGATG 1 cut(s) 616
BseJI GATNNNNATC 1 cut(s) 295
BseLI CCNNNNNNNGG 8 cut(s) 76, 93, 149, 343, 446, 479, 596, 642
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 643
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 1 cut(s) 359
BshFI GGCC 1 cut(s) 368
BsiSI CCGG 3 cut(s) 70, 77, 94
BslI CCNNNNNNNGG 8 cut(s) 76, 93, 149, 343, 446, 479, 596, 642
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 1 cut(s) 368
Bsp143I GATC 3 cut(s) 34, 90, 139
BspACI CCGC 1 cut(s) 591
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 1 cut(s) 642
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 2 cut(s) 85, 134
BspQI GCTCTTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 2 cut(s) 69, 76
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 2 cut(s) 69, 76
BssECI CCNNGG 3 cut(s) 275, 300, 595
BssMI GATC 3 cut(s) 34, 90, 139
BssSI CACGAG 1 cut(s) 370
BssT1I CCWWGG 3 cut(s) 275, 300, 595
Bst2BI CACGAG 1 cut(s) 370
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 2 cut(s) 344, 673
BstDEI CTNAG 1 cut(s) 629
BstEII GGTNACC 1 cut(s) 279
BstF5I GGATG 1 cut(s) 616
BstKTI GATC 3 cut(s) 37, 93, 142
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 3 cut(s) 34, 90, 139
BstPI GGTNACC 1 cut(s) 279
BstV2I GAAGAC 1 cut(s) 613
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 616
BtsIMutI CAGTG 1 cut(s) 558
CaiI CAGNNNCTG 1 cut(s) 560
Cfr10I RCCGGY 2 cut(s) 69, 76
Csp6I GTAC 2 cut(s) 386, 517
CviAII CATG 3 cut(s) 41, 617, 672
CviQI GTAC 2 cut(s) 386, 517
DdeI CTNAG 1 cut(s) 629
DpnI GATC 3 cut(s) 36, 92, 141
DpnII GATC 3 cut(s) 34, 90, 139
DraI TTTAAA 1 cut(s) 262
Eam1104I CTCTTC 2 cut(s) 344, 673
EarI CTCTTC 2 cut(s) 344, 673
Eco130I CCWWGG 3 cut(s) 275, 300, 595
Eco91I GGTNACC 1 cut(s) 279
EcoO65I GGTNACC 1 cut(s) 279
EcoT14I CCWWGG 3 cut(s) 275, 300, 595
ErhI CCWWGG 3 cut(s) 275, 300, 595
FaeI CATG 3 cut(s) 44, 620, 675
FaiI YATR 9 cut(s) 42, 53, 236, 405, 532, 537, 584, 618, 673
FatI CATG 3 cut(s) 40, 616, 671
FauI CCCGC 1 cut(s) 598
FokI GGATG 1 cut(s) 623
FspBI CTAG 2 cut(s) 392, 468
HaeIII GGCC 1 cut(s) 368
HapII CCGG 3 cut(s) 70, 77, 94
Hin1II CATG 3 cut(s) 44, 620, 675
HindIII AAGCTT 2 cut(s) 523, 572
HinfI GANTC 2 cut(s) 61, 436
HpaII CCGG 3 cut(s) 70, 77, 94
HphI GGTGA 5 cut(s) 152, 322, 407, 502, 611
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 3 cut(s) 139, 343, 632
Hpy188III TCNNGA 2 cut(s) 288, 433
Hpy8I GTNNAC 1 cut(s) 99
HpyAV CCTTC 4 cut(s) 173, 299, 343, 672
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 375
HpyF3I CTNAG 1 cut(s) 629
Hsp92II CATG 3 cut(s) 44, 620, 675
Kzo9I GATC 3 cut(s) 34, 90, 139
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 664
LpnPI CCDG 7 cut(s) 83, 90, 107, 167, 418, 432, 667
LweI GCATC 1 cut(s) 182
MaeI CTAG 2 cut(s) 392, 468
MaeIII GTNAC 3 cut(s) 279, 410, 490
MalI GATC 3 cut(s) 36, 92, 141
MboI GATC 3 cut(s) 34, 90, 139
MboII GAAGA 6 cut(s) 25, 56, 70, 361, 613, 660
MluCI AATT 2 cut(s) 146, 225
MnlI CCTC 8 cut(s) 153, 200, 215, 295, 337, 379, 481, 676
MseI TTAA 4 cut(s) 228, 261, 417, 654
MslI CAYNNNNRTG 2 cut(s) 45, 104
MspI CCGG 3 cut(s) 70, 77, 94
NdeII GATC 3 cut(s) 34, 90, 139
NlaIII CATG 3 cut(s) 44, 620, 675
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 61, 436
PflMI CCANNNNNTGG 3 cut(s) 446, 479, 642
PspEI GGTNACC 1 cut(s) 279
PspN4I GGNNCC 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 387, 518
RsaNI GTAC 2 cut(s) 386, 517
RseI CAYNNNNRTG 2 cut(s) 45, 104
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 4 cut(s) 228, 261, 417, 654
Sau3AI GATC 3 cut(s) 34, 90, 139
SfaNI GCATC 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 45, 104
Sse9I AATT 2 cut(s) 146, 225
SsiI CCGC 1 cut(s) 591
SspMI CTAG 2 cut(s) 392, 468
StyI CCWWGG 3 cut(s) 275, 300, 595
TaaI ACNGT 1 cut(s) 117
TaqI TCGA 3 cut(s) 11, 110, 289
TasI AATT 2 cut(s) 146, 225
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 2 cut(s) 61, 436
Tru1I TTAA 4 cut(s) 228, 261, 417, 654
Tru9I TTAA 4 cut(s) 228, 261, 417, 654
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 5 cut(s) 32, 57, 571, 605, 660
TspRI CASTG 1 cut(s) 565
Van91I CCANNNNNTGG 3 cut(s) 446, 479, 642
XspI CTAG 2 cut(s) 392, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.