pycom01g12020

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003384
Physical Location & Seq
Forward (+)
12958034 .. 12958888
855 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 795 bp
ATGATGAATCGAAATAATCTAACACATGTACTAGCTGTTATGAGGGGTTTGAGATGGATACTTGGGTGTATAATATTTGCATTCATGCATTTGGGTGATTTCGGTACTAGTCTGAGTCTGTTCAGAGAGATGAAATACTCTAACTTGGATTACGTCGTGCCGGATTTGTCGACATATAATAGCCTCATTCATGTGCTCTGTTTGGTGGGGAAGTTGAATGATGCACTTACTGTGTGGGAGGAATTGAAGGGCTCCGGCCATGAGCCTGACACAATTACCTATAGAATTCTTATTCAGGTGTGTTGTAAGTGCTATCGAATAGACGATGCCACTAAGATTTTTAGTGAAATGCAGCTCAATGGATATATCCCGGATACCATTGTTTATAATTCTCTAATAGATGGGTTATTCAAGGCTAGGAAGGTTAATGATGGCTGCCAGTTATTTGAGAAAATGGTTCAGAATGGGGCGAGAGCCTCGACGTGGACGTATAATATTCTCATGGATGGTTTGTTCAGGAATGGAAGGGCAGATGCTGCGTACACACTATTTTGTGACTTAAAGAAGAAGGGTCAGTTTGTTGATGGTGTAACATATAGCATTGTGGTTTTGCAACTTTGTAAGGACGGTCTGCTTGAGGAAGCGCTAGGATTGGTGGAAGAAATGGAAAGGAGAGGCTTTACTGTTGATTTAATTACTGTATCGTTACTTGTGATTGGGCTGTATAAAGAAGGTCGGTGGGATTGGACGGACAAGCTCATGAAGCACATTAGAGATGGTAACCTAGTTCCTTAA

Protein Analysis

265

Amino Acids

30.11

Weight (kDa)

6.22

Isoelectric Point (pI)

19.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 39 - 102 2.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 52 - 82 3.2e-08 PPR repeat
PPR_2 PF13041 54 - 103 7.2e-15 PPR repeat family
PPR PF01535 57 - 86 1e-05 PPR repeat
PPR_1 PF12854 86 - 118 1.2e-09 PPR repeat
PPR_2 PF13041 97 - 136 3.3e-08 PPR repeat family
PPR_3 PF13812 112 - 169 3.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 121 - 153 6.4e-09 PPR repeat
PPR_2 PF13041 124 - 172 1.9e-14 PPR repeat family
PPR PF01535 128 - 156 6.4e-06 PPR repeat
PPR_1 PF12854 159 - 185 7.9e-06 PPR repeat
PPR_2 PF13041 161 - 208 1.8e-12 PPR repeat family
PPR PF01535 163 - 191 4.2e-06 PPR repeat
PPR_1 PF12854 191 - 223 9.7e-07 PPR repeat
PPR PF01535 197 - 227 1.7e-06 PPR repeat
PPR_2 PF13041 206 - 243 9.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccI GTMKAC 1 cut(s) 170
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 3 cut(s) 30, 106, 542
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 1 cut(s) 483
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 3 cut(s) 217, 247, 412
AhlI ACTAGT 1 cut(s) 107
AjiI CACGTC 1 cut(s) 483
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
AluBI AGCT 3 cut(s) 35, 355, 757
AluI AGCT 3 cut(s) 35, 355, 757
Alw21I GWGCWC 1 cut(s) 198
AlwNI CAGNNNCTG 1 cut(s) 536
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 3 cut(s) 352, 435, 536
ApoI RAATTY 1 cut(s) 285
AspLEI GCGC 1 cut(s) 646
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 107
BaeI ACNNNNGTAYC 4 cut(s) 50, 83, 366, 399
BanII GRGCYC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 364, 422, 523
BccI CCATC 6 cut(s) 48, 395, 425, 500, 578, 770
BciVI GTATCC 2 cut(s) 51, 367
BcnI CCSGG 1 cut(s) 371
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 5 cut(s) 32, 108, 417, 647, 785
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 647
BfuI GTATCC 2 cut(s) 51, 367
BisI GCNGC 3 cut(s) 353, 436, 537
BlsI GCNGC 3 cut(s) 354, 437, 538
Bme1390I CCNGG 1 cut(s) 371
BmgBI CACGTC 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 3 cut(s) 211, 316, 523
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 371
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse1I ACTGG 1 cut(s) 439
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 439
BseXI GCAGC 3 cut(s) 364, 422, 523
BshFI GGCC 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 3 cut(s) 161, 255, 371
BslI CCNNNNNNNGG 1 cut(s) 483
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 198, 254
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 105
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 439
Bst4CI ACNGT 4 cut(s) 232, 629, 685, 700
BstAPI GCANNNNNTGC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 113, 333
BstEII GGTNACC 1 cut(s) 779
BstF5I GGATG 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 1 cut(s) 646
BstMWI GCNNNNNNNGC 2 cut(s) 536, 763
BstNSI RCATGY 1 cut(s) 29
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 364, 422, 523
BsuI GTATCC 2 cut(s) 51, 367
BsuRI GGCC 1 cut(s) 258
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 1 cut(s) 511
CaiI CAGNNNCTG 1 cut(s) 536
CciI TCATGA 1 cut(s) 759
CfoI GCGC 1 cut(s) 646
Csp6I GTAC 3 cut(s) 29, 105, 541
CviAII CATG 6 cut(s) 26, 85, 191, 260, 502, 760
CviQI GTAC 3 cut(s) 29, 105, 541
DdeI CTNAG 2 cut(s) 113, 333
EaeI YGGCCR 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 254
Eco47III AGCGCT 1 cut(s) 645
Eco91I GGTNACC 1 cut(s) 779
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 6 cut(s) 29, 88, 194, 263, 505, 763
FatI CATG 6 cut(s) 25, 84, 190, 259, 501, 759
FblI GTMKAC 1 cut(s) 170
Fnu4HI GCNGC 3 cut(s) 353, 436, 537
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 353, 436, 537
FspBI CTAG 5 cut(s) 32, 108, 417, 647, 785
GlaI GCGC 1 cut(s) 645
GluI GCNGC 3 cut(s) 353, 436, 537
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 1 cut(s) 258
HapII CCGG 3 cut(s) 161, 255, 371
HhaI GCGC 1 cut(s) 646
Hin1II CATG 6 cut(s) 29, 88, 194, 263, 505, 763
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 2 cut(s) 7, 115
HpaII CCGG 3 cut(s) 161, 255, 371
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 3 cut(s) 171, 486, 543
Hpy188I TCNGA 3 cut(s) 114, 125, 462
Hpy188III TCNNGA 2 cut(s) 517, 760
Hpy8I GTNNAC 3 cut(s) 171, 486, 543
Hpy99I CGWCG 2 cut(s) 158, 484
HpyAV CCTTC 5 cut(s) 241, 415, 519, 562, 725
HpyCH4III ACNGT 4 cut(s) 232, 629, 685, 700
HpyCH4IV ACGT 3 cut(s) 153, 482, 488
HpyCH4V TGCA 5 cut(s) 80, 88, 224, 352, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 763
HpyF3I CTNAG 2 cut(s) 113, 333
HpySE526I ACGT 3 cut(s) 153, 482, 488
Hsp92II CATG 6 cut(s) 29, 88, 194, 263, 505, 763
HspAI GCGC 1 cut(s) 644
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 7 cut(s) 174, 268, 279, 281, 384, 452, 502
Lsp1109I GCAGC 3 cut(s) 364, 422, 523
LweI GCATC 3 cut(s) 211, 316, 523
MaeI CTAG 5 cut(s) 32, 108, 417, 647, 785
MaeII ACGT 3 cut(s) 153, 482, 488
MaeIII GTNAC 4 cut(s) 554, 589, 705, 779
MboII GAAGA 2 cut(s) 577, 671
MhlI GDGCHC 2 cut(s) 198, 254
MluCI AATT 5 cut(s) 242, 273, 285, 388, 693
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 6 cut(s) 36, 194, 232, 487, 631, 668
Mph1103I ATGCAT 1 cut(s) 90
MseI TTAA 4 cut(s) 426, 560, 692, 793
MslI CAYNNNNRTG 2 cut(s) 93, 191
MspI CCGG 3 cut(s) 161, 255, 371
MspR9I CCNGG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 536, 763
NciI CCSGG 1 cut(s) 371
NlaIII CATG 6 cut(s) 29, 88, 194, 263, 505, 763
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 554
NsiI ATGCAT 1 cut(s) 90
NspI RCATGY 1 cut(s) 29
PagI TCATGA 1 cut(s) 759
PciI ACATGT 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 7
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 3 cut(s) 354, 437, 538
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PscI ACATGT 1 cut(s) 25
PsiI TTATAA 1 cut(s) 387
PspEI GGTNACC 1 cut(s) 779
PspN4I GGNNCC 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 536
RsaI GTAC 3 cut(s) 30, 106, 542
RsaNI GTAC 3 cut(s) 29, 105, 541
RseI CAYNNNNRTG 2 cut(s) 93, 191
SalI GTCGAC 1 cut(s) 169
SaqAI TTAA 4 cut(s) 426, 560, 692, 793
SatI GCNGC 3 cut(s) 353, 436, 537
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 2 cut(s) 198, 254
SfaNI GCATC 3 cut(s) 211, 316, 523
SfcI CTRYAG 1 cut(s) 280
SmiMI CAYNNNNRTG 2 cut(s) 93, 191
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 5 cut(s) 242, 273, 285, 388, 693
SspI AATATT 2 cut(s) 75, 496
SspMI CTAG 5 cut(s) 32, 108, 417, 647, 785
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 232, 629, 685, 700
TaiI ACGT 3 cut(s) 156, 485, 491
TaqI TCGA 4 cut(s) 10, 170, 316, 479
TasI AATT 5 cut(s) 242, 273, 285, 388, 693
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 4 cut(s) 426, 560, 692, 793
Tru9I TTAA 4 cut(s) 426, 560, 692, 793
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 3 cut(s) 352, 435, 536
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 5 cut(s) 20, 73, 146, 179, 776
TspGWI ACGGA 1 cut(s) 764
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 170
XspI CTAG 5 cut(s) 32, 108, 417, 647, 785
Zsp2I ATGCAT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.