pycom01g15950

Protein TIME FOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003700
Physical Location & Seq
Forward (+)
15692247 .. 15692729
483 bp
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UTR
Exon/CDS
Intron
pycom01g15950.1

Sequence Viewer

Length: 483 bp
ATGGAGGTGTTGTACGGAATGAAGAGGCAGCCGCAAGGATCGACGAAGCAAGAAATTACAGCCACCGATTCGATTAAGTTTGAATCCAGAGAAACCAACAACAAATTTACCAGCGACGCCAAATCGAGAGTTTCATCGCCGATCTCGAACTCGCCTTGTGTGGTTCCTCAACTGATGTCGCCTTTCCCTCATAATTCTTGCTCATTCGTCACGTCCATGTCCGCCGCCGGTGGATACTATAAGCTCACTGAACTCTCGGTTTTGTTCATTTCTTTCAGGGTACCCCAAATGCTCTGTTTGGTTGCTGAGGGACTGAGAAAAGAGATTGAATTAGTTTTTTGGTTAGCAAACAGAGCTAGGTTCTTTGTGTTTTTATGGTGTGATGAAAATTTAGTGTCTTGGCTTGAATCCGCAGCACCCAAGAGGAAAAGACCGCGACCCGTGAAGTATGAAGACGAGAACCCATCAATTTTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.21

Weight (kDa)

8.94

Isoelectric Point (pI)

71.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 280
AccB1I GGYRCC 1 cut(s) 280
AccII CGCG 1 cut(s) 436
AciI CCGC 5 cut(s) 32, 222, 225, 411, 434
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 2 cut(s) 104, 388
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 2 cut(s) 14, 282
AgsI TTSAA 3 cut(s) 83, 329, 407
AjiI CACGTC 1 cut(s) 213
AluBI AGCT 2 cut(s) 244, 356
AluI AGCT 2 cut(s) 244, 356
AlwI GGATC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 28, 413
ApoI RAATTY 2 cut(s) 104, 388
Asp718I GGTACC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 40, 425
BccI CCATC 1 cut(s) 472
BciVI GTATCC 1 cut(s) 227
BfaI CTAG 1 cut(s) 357
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 4 cut(s) 29, 32, 225, 414
BlsI GCNGC 4 cut(s) 30, 33, 226, 415
BmgBI CACGTC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 165, 282
BpiI GAAGAC 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 117
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 227
BseMII CTCAG 2 cut(s) 297, 305
BseXI GCAGC 2 cut(s) 40, 425
Bsh1236I CGCG 1 cut(s) 436
BshNI GGYRCC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 324
Bsp143I GATC 2 cut(s) 38, 141
BspACI CCGC 5 cut(s) 32, 222, 225, 411, 434
BspCNI CTCAG 2 cut(s) 298, 306
BspFNI CGCG 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 165, 282
BspPI GGATC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 227
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 2 cut(s) 38, 141
BssNI GRCGYC 1 cut(s) 117
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 306, 314
BstFNI CGCG 1 cut(s) 436
BstKTI GATC 2 cut(s) 41, 144
BstMBI GATC 2 cut(s) 38, 141
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 40, 425
BstV2I GAAGAC 1 cut(s) 459
BsuI GTATCC 1 cut(s) 227
BtgZI GCGATG 1 cut(s) 120
BtrI CACGTC 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 227
CseI GACGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 13, 281
CviAII CATG 1 cut(s) 217
CviJI RGCY 5 cut(s) 31, 62, 244, 356, 403
CviKI_1 RGCY 5 cut(s) 31, 62, 244, 356, 403
CviQI GTAC 2 cut(s) 13, 281
DdeI CTNAG 2 cut(s) 306, 314
DpnI GATC 2 cut(s) 40, 143
DpnII GATC 2 cut(s) 38, 141
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 1 cut(s) 211
FaeI CATG 1 cut(s) 220
FaiI YATR 5 cut(s) 192, 218, 240, 376, 450
FaqI GGGAC 1 cut(s) 324
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 29, 32, 225, 414
Fsp4HI GCNGC 4 cut(s) 29, 32, 225, 414
FspBI CTAG 1 cut(s) 357
GluI GCNGC 4 cut(s) 29, 32, 225, 414
HapII CCGG 1 cut(s) 228
HgaI GACGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 220
HinfI GANTC 3 cut(s) 68, 83, 407
HpaII CCGG 1 cut(s) 228
Hpy188III TCNNGA 3 cut(s) 87, 126, 145
Hpy99I CGWCG 2 cut(s) 46, 119
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 1 cut(s) 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 2 cut(s) 306, 314
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 220
KpnI GGTACC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 38, 141
LpnPI CCDG 4 cut(s) 100, 124, 241, 262
Lsp1109I GCAGC 2 cut(s) 40, 425
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 40, 143
MboI GATC 2 cut(s) 38, 141
MboII GAAGA 2 cut(s) 34, 464
MluCI AATT 6 cut(s) 54, 104, 193, 329, 388, 468
MnlI CCTC 5 cut(s) 18, 177, 198, 301, 417
MseI TTAA 2 cut(s) 75, 481
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 1 cut(s) 353
NdeII GATC 2 cut(s) 38, 141
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 2 cut(s) 165, 282
NmuCI GTSAC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PfeI GAWTC 3 cut(s) 68, 83, 407
PkrI GCNGC 4 cut(s) 30, 33, 226, 415
PspN4I GGNNCC 2 cut(s) 165, 282
RsaI GTAC 2 cut(s) 14, 282
RsaNI GTAC 2 cut(s) 13, 281
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 75, 481
SatI GCNGC 4 cut(s) 29, 32, 225, 414
Sau3AI GATC 2 cut(s) 38, 141
SetI ASST 5 cut(s) 9, 215, 246, 358, 362
SgrAI CRCCGGYG 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 6 cut(s) 54, 104, 193, 329, 388, 468
SsiI CCGC 5 cut(s) 32, 222, 225, 411, 434
SspMI CTAG 1 cut(s) 357
TaiI ACGT 1 cut(s) 215
TaqI TCGA 4 cut(s) 41, 71, 125, 146
TasI AATT 6 cut(s) 54, 104, 193, 329, 388, 468
TauI GCSGC 2 cut(s) 34, 227
TfiI GAWTC 3 cut(s) 68, 83, 407
Tru1I TTAA 2 cut(s) 75, 481
Tru9I TTAA 2 cut(s) 75, 481
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 28, 413
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 5 cut(s) 35, 123, 256, 399, 465
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 253
XapI RAATTY 2 cut(s) 104, 388
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.