Rw2G022720

carboxylesterase 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003700
Physical Location & Seq
Forward (+)
32596256 .. 32600824
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022720.1

Sequence Viewer

Length: 576 bp
ATGGGCGACGCCACCGAGTTGAAGAAGACCAATAATCGGCATATTATGGACACTTTCGGGGCTTCAATGGCTGAGCCGTGTCTTGGAGCCGTTTTCCCAACTAAAATGAATGTCAAGGCTGGTTCTGGTGGCAATGCTTTATCTTCTGAGCTCATGGATCAAGGTCGTCGCCGGCACGATGGTGAGATTGTTGGTTGGGGTATAGTTATTAAGAGAGAACAGAGAGCAGAGCACTGGTTCCTAGTGGTTAGCAGCAGCACAACACTAGGGTTGTTCTTGTTCCCTGTGCTTGTCGCTCGTGCGCTTCAAAATCCTTCAGCGCAGTCAGATCTCAAGGCTTCTCTTCTATCTTCACCTTTCCTTCCTCCGCCATTCTCTCTTTCGAAGCCAAGCTGTGCCAACCCCTCACTGGAGGGTCGGTGGATACTGTTGAATATAGATGGTGTTGCTTGGCATTCAAACCGAGTTGAATTACTGGATGTGGTGTGTGGAATGTGGAAGGATGACTCATTGGTTTGCATAGTTAAGGAGAGAAACTCGGTCCGTGATTGTGGGCTTTGGTGGGTATTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

191

Amino Acids

21.05

Weight (kDa)

8.32

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 368
AclWI GGATC 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 9
AfiI CCNNNNNNNGG 3 cut(s) 36, 83, 409
AgsI TTSAA 6 cut(s) 22, 66, 308, 433, 459, 470
AleI CACNNNNGTG 1 cut(s) 180
AluBI AGCT 2 cut(s) 151, 393
AluI AGCT 2 cut(s) 151, 393
Alw21I GWGCWC 2 cut(s) 153, 234
AlwI GGATC 1 cut(s) 165
ApeKI GCWGC 2 cut(s) 252, 255
AspLEI GCGC 2 cut(s) 304, 322
AspS9I GGNCC 1 cut(s) 541
AsuHPI GGTGA 2 cut(s) 194, 345
AsuII TTCGAA 1 cut(s) 383
AvaII GGWCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 153, 234
BbvI GCAGC 2 cut(s) 264, 267
BccI CCATC 2 cut(s) 173, 434
BceAI ACGGC 2 cut(s) 61, 74
BciVI GTATCC 1 cut(s) 417
BfaI CTAG 2 cut(s) 242, 266
BfuI GTATCC 1 cut(s) 417
BglII AGATCT 1 cut(s) 328
BisI GCNGC 2 cut(s) 253, 256
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 254, 257
Bme18I GGWCC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 2 cut(s) 88, 239
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 521, 553
BpmI CTGGAG 1 cut(s) 431
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 83, 409
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 3 cut(s) 239, 414, 480
Bse3DI GCAATG 1 cut(s) 139
BseGI GGATG 2 cut(s) 484, 508
BseLI CCNNNNNNNGG 3 cut(s) 36, 83, 409
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 63, 138
BseNI ACTGG 3 cut(s) 239, 414, 480
BseXI GCAGC 2 cut(s) 264, 267
BsiHKAI GWGCWC 2 cut(s) 153, 234
BsiSI CCGG 1 cut(s) 172
BslI CCNNNNNNNGG 3 cut(s) 36, 83, 409
BsmI GAATGC 1 cut(s) 454
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 153, 234
Bsp143I GATC 2 cut(s) 157, 328
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 1 cut(s) 368
BspCNI CTCAG 2 cut(s) 64, 139
BspLI GGNNCC 2 cut(s) 88, 239
BspPI GGATC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 3 cut(s) 239, 414, 480
BssAI RCCGGY 1 cut(s) 171
BssMI GATC 2 cut(s) 157, 328
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 348
BstACI GRCGYC 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 72, 147
BstF5I GGATG 2 cut(s) 484, 508
BstHHI GCGC 2 cut(s) 304, 322
BstKTI GATC 2 cut(s) 160, 331
BstMBI GATC 2 cut(s) 157, 328
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 264, 267
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuI GTATCC 1 cut(s) 417
BtsCI GGATG 2 cut(s) 484, 508
BtsIMutI CAGTG 2 cut(s) 232, 407
Cac8I GCNNGC 1 cut(s) 173
CfoI GCGC 2 cut(s) 304, 322
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 541
CpoI CGGWCCG 1 cut(s) 541
CseI GACGC 1 cut(s) 17
CspI CGGWCCG 1 cut(s) 541
CviAII CATG 1 cut(s) 154
DdeI CTNAG 2 cut(s) 72, 147
DpnI GATC 2 cut(s) 159, 330
DpnII GATC 2 cut(s) 157, 328
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 1 cut(s) 157
FaiI YATR 6 cut(s) 42, 47, 155, 203, 437, 521
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 253, 256
FokI GGATG 2 cut(s) 491, 515
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 2 cut(s) 253, 256
FspBI CTAG 2 cut(s) 242, 266
GlaI GCGC 2 cut(s) 303, 321
GluI GCNGC 2 cut(s) 253, 256
GsuI CTGGAG 1 cut(s) 431
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 17
HhaI GCGC 2 cut(s) 304, 322
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 2 cut(s) 302, 320
HinP1I GCGC 2 cut(s) 302, 320
HinfI GANTC 1 cut(s) 506
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 194, 345
Hpy188I TCNGA 2 cut(s) 148, 328
Hpy99I CGWCG 2 cut(s) 11, 171
HpyAV CCTTC 3 cut(s) 324, 371, 493
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4V TGCA 1 cut(s) 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 2 cut(s) 72, 147
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 2 cut(s) 302, 320
KroI GCCGGC 1 cut(s) 171
KroNI GCCGGC 1 cut(s) 173
Kzo9I GATC 2 cut(s) 157, 328
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 7 cut(s) 105, 111, 185, 220, 297, 395, 461
Lsp1109I GCAGC 2 cut(s) 264, 267
MaeI CTAG 2 cut(s) 242, 266
MalI GATC 2 cut(s) 159, 330
MboI GATC 2 cut(s) 157, 328
MboII GAAGA 5 cut(s) 34, 37, 135, 335, 342
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 153, 234
MluCI AATT 1 cut(s) 470
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 3 cut(s) 375, 406, 415
MroNI GCCGGC 1 cut(s) 171
MseI TTAA 3 cut(s) 210, 525, 569
MslI CAYNNNNRTG 1 cut(s) 180
MspI CCGG 1 cut(s) 172
Mva1269I GAATGC 1 cut(s) 454
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 173
NdeII GATC 2 cut(s) 157, 328
NgoMIV GCCGGC 1 cut(s) 171
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 2 cut(s) 88, 239
NspV TTCGAA 1 cut(s) 383
OliI CACNNNNGTG 1 cut(s) 180
PctI GAATGC 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 254, 257
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 88, 239
PspPI GGNCC 1 cut(s) 541
PsuI RGATCY 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 180
Rsr2I CGGWCCG 1 cut(s) 541
RsrII CGGWCCG 1 cut(s) 541
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 3 cut(s) 210, 525, 569
SatI GCNGC 2 cut(s) 253, 256
Sau3AI GATC 2 cut(s) 157, 328
Sau96I GGNCC 1 cut(s) 541
SchI GAGTC 1 cut(s) 500
SduI GDGCHC 2 cut(s) 153, 234
SetI ASST 4 cut(s) 153, 166, 358, 395
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 1 cut(s) 470
SsiI CCGC 1 cut(s) 368
SspMI CTAG 2 cut(s) 242, 266
SstI GAGCTC 1 cut(s) 153
TaaI ACNGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 383
TaqII GACCGA 1 cut(s) 529
TasI AATT 1 cut(s) 470
Tru1I TTAA 3 cut(s) 210, 525, 569
Tru9I TTAA 3 cut(s) 210, 525, 569
TscAI CASTG 2 cut(s) 239, 414
TseI GCWGC 2 cut(s) 252, 255
TspDTI ATGAA 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 2 cut(s) 239, 414
VpaK11BI GGWCC 1 cut(s) 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 2 cut(s) 242, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.