pycom01g19200

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002381
Physical Location & Seq
Forward (+)
17978033 .. 17980857
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGAAAATGTCATTGACAGTCCTTGGAAAAAATTTCATTCATTCTCTCCCCTCCCACACACGCAACTCTCTTTCTCTGCAACTCCACCCTCCAACTGCAACAACAGTGGGATTCCACCCAGACACCCAGAAGATGAACAACAACAACAAGGGCAAAGCAGTCGCCGTCTCAAGCCCCAACGTCAACGACATCGACGACGACGACGACGGCAACGGTAACATCAGCGAAGACGAATTGGACGACGACAACAACAGAAGCAGTGGCGTCTCTTCGACTTCCATCAGATTCTCGTCCCGCAACACTTCCTCCAAATACGACTTCGTCAAGGTGAAGGTCTGGCTGGGTGATAATGCAGACCACTACTACGTTCTGTCCAGATTTTTGCTCAGTCGAATGTTGACTGTCACTAAGATCCCAAATTATGTAGCGATTAAAATAGCGCTGGAGCTCAAGAAGTTGCTGATTGACAACAGTCTGCTAGATGTTTCGCAGTCCGATTTAGAAGTCAATTTGTTTAAGCTTATGGAACGAAGGGGATATGGGGAAGAGTACATAAATCGGTACAAGATGATGACCAGATTTCACCATCAACGAGTGCCATTAGTTATTCTTGTATGTGGAACTGCCTGTGTTGGAAAGTCGACCATTGCTACCCAACTCGCACAAAGATTAAATTTGCCTAATGTCTTGCAGACGGATATGGTATATGAATTGTTGCGCACGGCAACAGATGCACCGTTGACATCTACTCCTGTATGGGCACGAGATTTCAGCTCCTCTGAGGAATTAATTACTGAATTTTGTAGGGAATGCAGGATTGTCCGTAAAGGATTGGGTGGTGATATGAAGAAAGCTATGAAAGATGGAAAGCCAATAATAATAGAGTTTTGTAATGCTGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

302

Amino Acids

33.84

Weight (kDa)

7.65

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 203 - 300 1.6e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 719
AccI GTMKAC 1 cut(s) 641
AciI CCGC 1 cut(s) 295
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 3 cut(s) 31, 673, 797
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 551, 563
AfeI AGCGCT 1 cut(s) 441
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 4 cut(s) 448, 520, 774, 854
AluI AGCT 4 cut(s) 448, 520, 774, 854
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 172, 271
AlwI GGATC 1 cut(s) 406
Aor51HI AGCGCT 1 cut(s) 441
ApoI RAATTY 3 cut(s) 31, 673, 797
AseI ATTAAT 1 cut(s) 788
AspLEI GCGC 2 cut(s) 442, 720
AsuHPI GGTGA 4 cut(s) 340, 356, 575, 851
BaeGI GKGCMC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 450
BauI CACGAG 1 cut(s) 762
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 450
BccI CCATC 3 cut(s) 287, 594, 857
BceAI ACGGC 3 cut(s) 149, 223, 738
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BcoDI GTCTC 2 cut(s) 172, 271
BfaI CTAG 1 cut(s) 479
BfoI RGCGCY 1 cut(s) 443
BmsI GCATC 1 cut(s) 721
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 464
BpuEI CTTGAG 2 cut(s) 154, 434
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 733, 763
Bse3DI GCAATG 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 22
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 400, 771
BseRI GAGGAG 1 cut(s) 766
BseSI GKGCMC 1 cut(s) 763
BseYI CCCAGC 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 450
BslFI GGGAC 1 cut(s) 277
BsmAI GTCTC 2 cut(s) 172, 271
BsmBI CGTCTC 2 cut(s) 172, 271
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 815
Bsp1286I GDGCHC 2 cut(s) 450, 763
Bsp143I GATC 1 cut(s) 411
BspACI CCGC 1 cut(s) 295
BspCNI CTCAG 2 cut(s) 399, 772
BspPI GGATC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 645
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 411
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 762
Bst4CI ACNGT 6 cut(s) 19, 106, 215, 403, 473, 738
Bst6I CTCTTC 2 cut(s) 274, 540
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 731
BstDEI CTNAG 3 cut(s) 386, 408, 780
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 2 cut(s) 442, 720
BstKTI GATC 1 cut(s) 414
BstMAI GTCTC 2 cut(s) 172, 271
BstMBI GATC 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 731
BstPAI GACNNNNGTC 1 cut(s) 471
BstSLI GKGCMC 1 cut(s) 763
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 411
BstYI RGATCY 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 2 cut(s) 111, 265
CfoI GCGC 2 cut(s) 442, 720
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 550, 562
CspCI CAANNNNNGTGG 2 cut(s) 87, 122
CviJI RGCY 7 cut(s) 174, 340, 448, 520, 774, 854, 871
CviKI_1 RGCY 7 cut(s) 174, 340, 448, 520, 774, 854, 871
CviQI GTAC 2 cut(s) 550, 562
DdeI CTNAG 3 cut(s) 386, 408, 780
DpnI GATC 1 cut(s) 413
DpnII GATC 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 274, 540
EarI CTCTTC 2 cut(s) 274, 540
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 450
Eco47III AGCGCT 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 22
Esp3I CGTCTC 2 cut(s) 172, 271
FaqI GGGAC 1 cut(s) 277
FauI CCCGC 1 cut(s) 302
FblI GTMKAC 1 cut(s) 641
FriOI GRGCYC 1 cut(s) 450
FspBI CTAG 1 cut(s) 479
FspI TGCGCA 1 cut(s) 719
GlaI GCGC 2 cut(s) 441, 719
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 443
HgaI GACGC 1 cut(s) 253
HhaI GCGC 2 cut(s) 442, 720
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 2 cut(s) 440, 718
HinP1I GCGC 2 cut(s) 440, 718
HincII GTYRAC 4 cut(s) 184, 399, 642, 741
HindII GTYRAC 4 cut(s) 184, 399, 642, 741
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 2 cut(s) 111, 285
HphI GGTGA 4 cut(s) 340, 356, 575, 851
Hpy166II GTNNAC 4 cut(s) 184, 399, 642, 741
Hpy188I TCNGA 3 cut(s) 284, 496, 781
Hpy188III TCNNGA 2 cut(s) 375, 451
Hpy8I GTNNAC 4 cut(s) 184, 399, 642, 741
Hpy99I CGWCG 6 cut(s) 197, 200, 203, 206, 209, 245
HpyAV CCTTC 2 cut(s) 325, 525
HpyCH4III ACNGT 6 cut(s) 19, 106, 215, 403, 473, 738
HpyCH4IV ACGT 2 cut(s) 180, 366
HpyCH4V TGCA 6 cut(s) 79, 98, 353, 691, 734, 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 731
HpyF3I CTNAG 3 cut(s) 386, 408, 780
HpySE526I ACGT 2 cut(s) 180, 366
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 2 cut(s) 440, 718
Kzo9I GATC 1 cut(s) 411
LmnI GCTCC 2 cut(s) 445, 779
LweI GCATC 1 cut(s) 721
MaeI CTAG 1 cut(s) 479
MaeII ACGT 2 cut(s) 180, 366
MaeIII GTNAC 2 cut(s) 215, 403
MalI GATC 1 cut(s) 413
MboI GATC 1 cut(s) 411
MboII GAAGA 5 cut(s) 142, 239, 261, 557, 859
MflI RGATCY 1 cut(s) 411
MhlI GDGCHC 2 cut(s) 450, 763
MluCI AATT 9 cut(s) 31, 233, 418, 508, 673, 710, 785, 789, 797
MmeI TCCRAC 2 cut(s) 116, 613
MnlI CCTC 5 cut(s) 61, 99, 316, 775, 787
MseI TTAA 4 cut(s) 432, 516, 671, 788
Mva1269I GAATGC 1 cut(s) 815
MwoI GCNNNNNNNGC 1 cut(s) 731
NdeII GATC 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 403
NsbI TGCGCA 1 cut(s) 719
PcsI WCGNNNNNNNCGW 4 cut(s) 192, 198, 201, 237
PctI GAATGC 1 cut(s) 815
PfeI GAWTC 2 cut(s) 111, 285
PflFI GACNNNGTC 1 cut(s) 320
PshAI GACNNNNGTC 1 cut(s) 471
PshBI ATTAAT 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 340
PsuI RGATCY 1 cut(s) 411
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 2 cut(s) 551, 563
RsaNI GTAC 2 cut(s) 550, 562
SacI GAGCTC 1 cut(s) 450
SalI GTCGAC 1 cut(s) 640
SaqAI TTAA 4 cut(s) 432, 516, 671, 788
Sau3AI GATC 1 cut(s) 411
SduI GDGCHC 2 cut(s) 450, 763
SetI ASST 8 cut(s) 183, 330, 336, 369, 450, 522, 776, 856
SfaNI GCATC 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 169, 449
SmoI CTYRAG 2 cut(s) 169, 449
Sse9I AATT 9 cut(s) 31, 233, 418, 508, 673, 710, 785, 789, 797
SsiI CCGC 1 cut(s) 295
SspMI CTAG 1 cut(s) 479
SstI GAGCTC 1 cut(s) 450
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 6 cut(s) 19, 106, 215, 403, 473, 738
TaiI ACGT 2 cut(s) 183, 369
TaqI TCGA 4 cut(s) 192, 272, 391, 641
TasI AATT 9 cut(s) 31, 233, 418, 508, 673, 710, 785, 789, 797
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 2 cut(s) 111, 285
Tru1I TTAA 4 cut(s) 432, 516, 671, 788
Tru9I TTAA 4 cut(s) 432, 516, 671, 788
TscAI CASTG 2 cut(s) 111, 265
TseFI GTSAC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 7 cut(s) 17, 25, 29, 149, 723, 860, 872
TspGWI ACGGA 2 cut(s) 710, 812
TspRI CASTG 2 cut(s) 111, 265
Tth111I GACNNNGTC 1 cut(s) 320
VspI ATTAAT 1 cut(s) 788
XapI RAATTY 3 cut(s) 31, 673, 797
XmiI GTMKAC 1 cut(s) 641
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.