pycom01g24270

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002676
Physical Location & Seq
Forward (+)
21232535 .. 21234836
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGCTGATCGATCAACAGGAGGCCATGCTTTCCGACGGCGTAGATAATCTAGGTAAAGAGTCGAAGCTGGAGGTTTCTGAAACAGAAGTTAGAGGCGTACTACAAGTTATTGCATCCACCGGGAAATTTTGGCATGATTGGGACAAATTAAAGAGCATGCTATCCTTTCAACTGAAGCAGGTTCTGTTGGATTATCCTGAGGAAGAAATGGCAAGTGAGCAGCAAATTGCTTCTCTAGGAGAAACCTATCCTGAGCTGGTGAAGAGGTTGGATGAAGCTCTTCATAGTTTTACTGAAGGTCCTCCGTTTACCCTTCAGAGGCTTTGCGAGATCCTATTGGATGCAAAAAGAATCTATCCAAATCCCTCAAAGCTTGCTCTTGCATTAGAAAAGAATCTATTGGTGACATCTATGCTGACAGCCTCAACGGATCCATATCCACAATCCACGGTGCAAAATTCAGTTGAACCAGACAAAGCAATTGAAGAAGCTAAAATTCACTCTGATTCAGTAGCGCAAAATGGGGTGGAACCTATGGTAAGCGGCGATATGGATGAAGTTATGTCAGAGGTAGAGCAAGCTGATATCGATAATGATATGACCATCGCCATGGAAGCTTTTGAAGATATAGTGGGATCATCAGACGCAAATTCAGTGCAGACCAATGATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

225

Amino Acids

24.81

Weight (kDa)

4.17

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 27 - 163 1.6e-14 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 169
AciI CCGC 1 cut(s) 543
AclWI GGATC 4 cut(s) 325, 425, 438, 643
AcsI RAATTY 4 cut(s) 125, 457, 495, 649
AcuI CTGAAG 3 cut(s) 194, 299, 315
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 4 cut(s) 170, 467, 485, 623
AluBI AGCT 7 cut(s) 67, 256, 278, 373, 491, 581, 617
AluI AGCT 7 cut(s) 67, 256, 278, 373, 491, 581, 617
AlwI GGATC 4 cut(s) 325, 425, 438, 643
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 4 cut(s) 125, 457, 495, 649
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 299
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 2 cut(s) 271, 415
AvaII GGWCC 1 cut(s) 299
AxyI CCTNAGG 1 cut(s) 198
BamHI GGATCC 1 cut(s) 430
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 611
BceAI ACGGC 1 cut(s) 52
BcnI CCSGG 1 cut(s) 121
BfaI CTAG 2 cut(s) 50, 236
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 2 cut(s) 221, 544
BlsI GCNGC 2 cut(s) 222, 545
Bme1390I CCNGG 1 cut(s) 121
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 432, 531
BmrFI CCNGG 1 cut(s) 121
BmsI GCATC 2 cut(s) 122, 331
BpmI CTGGAG 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 121
Bsa29I ATCGAT 2 cut(s) 9, 588
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 447, 609
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse21I CCTNAGG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 435
BseCI ATCGAT 2 cut(s) 9, 588
BseDI CCNNGG 2 cut(s) 447, 609
BseGI GGATG 4 cut(s) 113, 277, 346, 559
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 189, 243
BseXI GCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 23
BshVI ATCGAT 2 cut(s) 9, 588
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 23
Bsp143I GATC 5 cut(s) 6, 10, 330, 430, 635
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 1 cut(s) 543
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 2 cut(s) 190, 244
BspDI ATCGAT 2 cut(s) 9, 588
BspLI GGNNCC 2 cut(s) 432, 531
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 4 cut(s) 325, 425, 438, 643
BspQI GCTCTTC 1 cut(s) 285
BssECI CCNNGG 2 cut(s) 447, 609
BssMI GATC 5 cut(s) 6, 10, 330, 430, 635
BssT1I CCWWGG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 2 cut(s) 257, 285
BstC8I GCNNGC 3 cut(s) 158, 375, 579
BstDEI CTNAG 3 cut(s) 198, 252, 672
BstDSI CCRYGG 2 cut(s) 447, 609
BstF5I GGATG 4 cut(s) 113, 277, 346, 559
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 5 cut(s) 9, 13, 333, 433, 638
BstMBI GATC 5 cut(s) 6, 10, 330, 430, 635
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 2 cut(s) 330, 430
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 2 cut(s) 330, 430
Bsu15I ATCGAT 2 cut(s) 9, 588
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 1 cut(s) 23
BsuTUI ATCGAT 2 cut(s) 9, 588
BtgI CCRYGG 2 cut(s) 447, 609
BtgZI GCGATG 1 cut(s) 589
BtsCI GGATG 4 cut(s) 113, 277, 346, 559
BtsIMutI CAGTG 1 cut(s) 660
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 3 cut(s) 158, 375, 579
CaiI CAGNNNCTG 1 cut(s) 184
CfoI GCGC 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 299
ClaI ATCGAT 2 cut(s) 9, 588
CseI GACGC 1 cut(s) 653
Csp6I GTAC 1 cut(s) 98
CviAII CATG 4 cut(s) 25, 134, 157, 610
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 3 cut(s) 198, 252, 672
DpnI GATC 5 cut(s) 8, 12, 332, 432, 637
DpnII GATC 5 cut(s) 6, 10, 330, 430, 635
Eam1104I CTCTTC 2 cut(s) 257, 285
EarI CTCTTC 2 cut(s) 257, 285
Eco130I CCWWGG 1 cut(s) 609
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 3 cut(s) 194, 299, 315
Eco81I CCTNAGG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 299
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 4 cut(s) 28, 137, 160, 613
FaqI GGGAC 1 cut(s) 155
FatI CATG 4 cut(s) 24, 133, 156, 609
Fnu4HI GCNGC 2 cut(s) 221, 544
FokI GGATG 4 cut(s) 100, 284, 353, 566
Fsp4HI GCNGC 2 cut(s) 221, 544
FspBI CTAG 2 cut(s) 50, 236
GlaI GCGC 1 cut(s) 516
GluI GCNGC 2 cut(s) 221, 544
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 653
HhaI GCGC 1 cut(s) 517
Hin1II CATG 4 cut(s) 28, 137, 160, 613
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HindIII AAGCTT 2 cut(s) 371, 615
HinfI GANTC 5 cut(s) 59, 351, 394, 506, 668
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 271, 415
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 6 cut(s) 34, 79, 318, 505, 568, 643
Hpy188III TCNNGA 2 cut(s) 197, 251
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 290, 323
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 5 cut(s) 113, 344, 383, 454, 658
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 3 cut(s) 198, 252, 672
Hsp92II CATG 4 cut(s) 28, 137, 160, 613
HspAI GCGC 1 cut(s) 515
Kzo9I GATC 5 cut(s) 6, 10, 330, 430, 635
LguI GCTCTTC 1 cut(s) 285
LpnPI CCDG 8 cut(s) 2, 53, 133, 164, 210, 242, 264, 483
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 2 cut(s) 122, 331
MaeI CTAG 2 cut(s) 50, 236
MaeIII GTNAC 1 cut(s) 403
MalI GATC 5 cut(s) 8, 12, 332, 432, 637
MboI GATC 5 cut(s) 6, 10, 330, 430, 635
MboII GAAGA 5 cut(s) 215, 272, 274, 497, 635
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 2 cut(s) 330, 430
MluCI AATT 7 cut(s) 125, 146, 225, 457, 480, 495, 649
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 3 cut(s) 57, 168, 249
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 1 cut(s) 121
MunI CAATTG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 614
NciI CCSGG 1 cut(s) 121
NcoI CCATGG 1 cut(s) 609
NdeII GATC 5 cut(s) 6, 10, 330, 430, 635
NlaIII CATG 4 cut(s) 28, 137, 160, 613
NlaIV GGNNCC 2 cut(s) 432, 531
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 285
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 4 cut(s) 351, 394, 506, 668
PkrI GCNGC 2 cut(s) 222, 545
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 432, 531
PspPI GGNCC 1 cut(s) 299
PspPPI RGGWCCY 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 330, 430
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 608
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 2 cut(s) 221, 544
Sau3AI GATC 5 cut(s) 6, 10, 330, 430, 635
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 121
SfaNI GCATC 2 cut(s) 122, 331
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 608
SphI GCATGC 1 cut(s) 160
Sse9I AATT 7 cut(s) 125, 146, 225, 457, 480, 495, 649
SsiI CCGC 1 cut(s) 543
SspMI CTAG 2 cut(s) 50, 236
StyD4I CCNGG 1 cut(s) 119
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 1 cut(s) 451
TaqI TCGA 3 cut(s) 9, 62, 588
TasI AATT 7 cut(s) 125, 146, 225, 457, 480, 495, 649
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 4 cut(s) 351, 394, 506, 668
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TscAI CASTG 1 cut(s) 660
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 3 cut(s) 272, 288, 570
TspGWI ACGGA 2 cut(s) 294, 443
TspRI CASTG 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 4 cut(s) 125, 457, 495, 649
XceI RCATGY 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 2 cut(s) 50, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.