pycom04g11380

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001568
Physical Location & Seq
Reverse (-)
14263834 .. 14265790
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11380.1

Sequence Viewer

Length: 669 bp
ATGGCTCACCAGGACGAAGGGTGGCCGTTGGGATTGAGACCGCTGAATGCGAGAGTTGGGTTGATCAGAAATCGGGATTTATCCGGTGGGTCGGTCTCATTCAGCACTTTGATCACTGCCTCTCCGACTTCCTCCATCGCTTCTTCTTCTTCTTCGTCCTCAGATCTTGAAACAGAGCCAACTGAATCTTTCTTCCATGACAAGGGCATCACACTGGGGAGCCTCATCGGCGTTTCCGGCATCCTAGATCTCTCCGGAAGATCTACACGGAGAAGGGCAACAGAAACCACCAAGAACAAAAAGAACCGTAAAACCAAACCTTGGTTGTTTTCCTTCTGCTCCAAGCTAACCAATGACTCTCTGAACGTGAGCAATGATACTCCATCACTAGGTCACTTTCTTGAGGCAGAAAGGAGAGCAAGTACCCACAACACCAACAGAAGAAATCACGTCGCTCCAATCGAATATGGCCCCGAAGATTTCTCACCGGCAGTGCCGGTTTCCGGCCCCAACGGCCTGTTTGTGGACGGTGGAGTTGCCGCTGCTCAGCCAAGTCCTTCAGAGAGTGGAGATGGTGGATCAAGATCCAACAGCGAGTTACTAGAATATGGCAATGGACTTGGAACTCCATTACTGCTTTCATGTTTGAGTGGAAAACTCATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

23.51

Weight (kDa)

7.83

Isoelectric Point (pI)

56.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 2 cut(s) 41, 540
AclWI GGATC 2 cut(s) 579, 586
AcoI YGGCCR 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 5 cut(s) 202, 321, 389, 503, 523
AgsI TTSAA 1 cut(s) 170
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 1 cut(s) 346
AluI AGCT 1 cut(s) 346
Alw26I GTCTC 2 cut(s) 31, 100
AlwI GGATC 2 cut(s) 579, 586
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 4 cut(s) 23, 469, 505, 514
ApeKI GCWGC 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 470, 506
AsuHPI GGTGA 1 cut(s) 477
BbvI GCAGC 1 cut(s) 529
BccI CCATC 3 cut(s) 143, 391, 566
BceAI ACGGC 2 cut(s) 10, 529
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 2 cut(s) 63, 111
BcoDI GTCTC 2 cut(s) 31, 100
BfaI CTAG 3 cut(s) 245, 389, 602
BglI GCCNNNNNGGC 2 cut(s) 228, 513
BglII AGATCT 3 cut(s) 163, 247, 260
BisI GCNGC 2 cut(s) 540, 543
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 2 cut(s) 541, 544
Bme1390I CCNGG 1 cut(s) 11
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 470, 506
BmiI GGNNCC 3 cut(s) 221, 472, 508
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 216, 249
BmuI ACTGGG 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 583
BsaI GGTCTC 2 cut(s) 31, 100
BsaJI CCNNGG 1 cut(s) 320
BsaWI WCCGGW 2 cut(s) 83, 254
BsaXI ACNNNNNCTCC 4 cut(s) 106, 136, 406, 436
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 321, 389, 503, 523
Bse118I RCCGGY 2 cut(s) 487, 496
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 379, 619
Bse8I GATNNNNATC 1 cut(s) 583
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 583
BseLI CCNNNNNNNGG 5 cut(s) 202, 321, 389, 503, 523
BseMI GCAATG 2 cut(s) 379, 619
BseMII CTCAG 2 cut(s) 174, 560
BseNI ACTGG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 529
BshFI GGCC 4 cut(s) 25, 471, 507, 516
BsiSI CCGG 6 cut(s) 84, 237, 255, 488, 497, 504
BslI CCNNNNNNNGG 5 cut(s) 202, 321, 389, 503, 523
BsmAI GTCTC 2 cut(s) 31, 100
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 4 cut(s) 25, 471, 507, 516
Bso31I GGTCTC 2 cut(s) 31, 100
Bsp13I TCCGGA 1 cut(s) 254
Bsp143I GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 2 cut(s) 41, 540
BspANI GGCC 4 cut(s) 25, 471, 507, 516
BspCNI CTCAG 2 cut(s) 173, 559
BspEI TCCGGA 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 221, 472, 508
BspPI GGATC 2 cut(s) 579, 586
BspTNI GGTCTC 2 cut(s) 31, 100
BsrDI GCAATG 2 cut(s) 379, 619
BsrFI RCCGGY 2 cut(s) 487, 496
BsrI ACTGG 1 cut(s) 219
BssAI RCCGGY 2 cut(s) 487, 496
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
BssT1I CCWWGG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 308, 530
BstDEI CTNAG 2 cut(s) 160, 546
BstF5I GGATG 1 cut(s) 240
BstKTI GATC 7 cut(s) 66, 114, 166, 250, 263, 581, 587
BstMAI GTCTC 2 cut(s) 31, 100
BstMBI GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
BstMWI GCNNNNNNNGC 3 cut(s) 228, 237, 513
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 529
BstX2I RGATCY 4 cut(s) 163, 247, 260, 584
BstYI RGATCY 4 cut(s) 163, 247, 260, 584
BsuRI GGCC 4 cut(s) 25, 471, 507, 516
BtgZI GCGATG 1 cut(s) 121
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 114, 498
BtsIMutI CAGTG 3 cut(s) 114, 212, 498
Cfr10I RCCGGY 2 cut(s) 487, 496
Cfr13I GGNCC 2 cut(s) 470, 506
Csp6I GTAC 1 cut(s) 423
CviAII CATG 2 cut(s) 197, 642
CviJI RGCY 9 cut(s) 5, 25, 178, 222, 346, 471, 507, 516, 550
CviKI_1 RGCY 9 cut(s) 5, 25, 178, 222, 346, 471, 507, 516, 550
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 2 cut(s) 160, 546
DpnI GATC 7 cut(s) 65, 113, 165, 249, 262, 580, 586
DpnII GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
EaeI YGGCCR 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 320
Eco31I GGTCTC 2 cut(s) 31, 100
Eco57I CTGAAG 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 2 cut(s) 200, 645
FaiI YATR 4 cut(s) 198, 468, 609, 643
FatI CATG 2 cut(s) 196, 641
FbaI TGATCA 2 cut(s) 63, 111
Fnu4HI GCNGC 2 cut(s) 540, 543
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 2 cut(s) 540, 543
FspBI CTAG 3 cut(s) 245, 389, 602
GluI GCNGC 2 cut(s) 540, 543
HaeIII GGCC 4 cut(s) 25, 471, 507, 516
HapII CCGG 6 cut(s) 84, 237, 255, 488, 497, 504
Hin1II CATG 2 cut(s) 200, 645
HinfI GANTC 2 cut(s) 185, 356
HpaII CCGG 6 cut(s) 84, 237, 255, 488, 497, 504
HphI GGTGA 1 cut(s) 477
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 5 cut(s) 68, 126, 163, 363, 562
Hpy188III TCNNGA 5 cut(s) 74, 167, 255, 401, 582
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 4 cut(s) 11, 267, 343, 567
HpyCH4III ACNGT 2 cut(s) 308, 530
HpyCH4IV ACGT 2 cut(s) 366, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 237, 513
HpyF3I CTNAG 2 cut(s) 160, 546
HpySE526I ACGT 2 cut(s) 366, 450
Hsp92II CATG 2 cut(s) 200, 645
Kpn2I TCCGGA 1 cut(s) 254
Ksp22I TGATCA 2 cut(s) 63, 111
Kzo9I GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
LmnI GCTCC 3 cut(s) 219, 344, 460
LpnPI CCDG 9 cut(s) 23, 97, 200, 250, 268, 501, 510, 517, 530
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 2 cut(s) 216, 249
MaeI CTAG 3 cut(s) 245, 389, 602
MaeII ACGT 2 cut(s) 366, 450
MaeIII GTNAC 2 cut(s) 392, 597
MalI GATC 7 cut(s) 65, 113, 165, 249, 262, 580, 586
MboI GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
MboII GAAGA 8 cut(s) 135, 138, 141, 144, 184, 270, 453, 488
MflI RGATCY 4 cut(s) 163, 247, 260, 584
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 149, 612
MnlI CCTC 5 cut(s) 130, 142, 169, 233, 397
MroI TCCGGA 1 cut(s) 254
MseI TTAA 1 cut(s) 663
MspA1I CMGCKG 2 cut(s) 43, 542
MspI CCGG 6 cut(s) 84, 237, 255, 488, 497, 504
MspR9I CCNGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 228, 237, 513
NdeII GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
NlaIII CATG 2 cut(s) 200, 645
NlaIV GGNNCC 3 cut(s) 221, 472, 508
NmuCI GTSAC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 2 cut(s) 541, 544
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 221, 472, 508
PspPI GGNCC 2 cut(s) 470, 506
PsuI RGATCY 4 cut(s) 163, 247, 260, 584
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SaqAI TTAA 1 cut(s) 663
SatI GCNGC 2 cut(s) 540, 543
Sau3AI GATC 7 cut(s) 63, 111, 163, 247, 260, 578, 584
Sau96I GGNCC 2 cut(s) 470, 506
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 11
SetI ASST 5 cut(s) 322, 348, 369, 394, 453
SfaNI GCATC 2 cut(s) 216, 249
SfiI GGCCNNNNNGGCC 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SsiI CCGC 2 cut(s) 41, 540
SspMI CTAG 3 cut(s) 245, 389, 602
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 2 cut(s) 308, 530
TaiI ACGT 2 cut(s) 369, 453
TaqI TCGA 1 cut(s) 462
TaqII GACCGA 1 cut(s) 82
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 3 cut(s) 121, 219, 498
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 630
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 3 cut(s) 121, 219, 498
Van91I CCANNNNNTGG 1 cut(s) 321
XspI CTAG 3 cut(s) 245, 389, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.