pycom11g09130

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001568
Physical Location & Seq
Forward (+)
7285388 .. 7287050
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09130.5

Sequence Viewer

Length: 864 bp
ATGATTCTGTTTCCTCTTTCTAGTCAAACACTCAACAGGAGTCAGGACCCAAGAAAAGCAAACAAACAGTGCTCACAAATGATTAGTCCACTTTCTCTCTCTCTCTCTCTCCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTACATTCCAGGGTTACCCAATATTACCACCCCAACAAGAATTCACCTCAGAAAAAGCAAAACTGCAATACAAATGGCTCAGCAACAACAGCAGGGCAGGAAGGGTGGCCACTGGGACTGCAGCCATTGCATGTGAGAGTTGGGCTGGCTGCTTCAAACCATGAATTTTCTGGGTCGGTTTCTTTTAACACTTTACTCACTGGTTCTCCAACTTCCTCCACAGATTCCTCATCAGATCTGGACACAGAGTCCACAGGGTCTTTTTTCCATGATAGGAGCATTACGCTCGGGAGCCTTATAGGAATCAATAACATCCTAGAGCTCTCAAGAAGATCTCTAAGAGGAAGAAAAACTGATCATCAGGCCGGCAAGGACAGGAAGATGAACAACAACAACAACAACAACACCAAATTCAGACTCTGTTTTCTCTCTTTATGCTCCAAAGACAGTACAGATGATGAGAACGTGCGCAATCATCCGCCATCTCTCGGCCACTTTCTTGCGGCTGAGAGACAAGCGGCCAATGAAAACTACAGAACAAACCCTCCAATTTTCGGACCAGACGACGAGCTTGCCCTAGCTGAGCATCATCAGCCTGCTAGAGAATCAAATAACTCACTCTTTGTTGATGGTCAAGTTGCTCCTCCCCAATCTAGTCCACGGTTTGGTTCAGGTCATGTCGACCAAAGAAAATCAATGCTGTTTTCATGCATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

31.49

Weight (kDa)

8.77

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 809
AccI GTMKAC 1 cut(s) 825
AciI CCGC 3 cut(s) 623, 647, 662
AcoI YGGCCR 3 cut(s) 251, 634, 663
AcsI RAATTY 3 cut(s) 184, 308, 554
AfaI GTAC 1 cut(s) 595
AfiI CCNNNNNNNGG 3 cut(s) 632, 698, 809
AgsI TTSAA 1 cut(s) 300
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 152
AloI GAACNNNNNNTCC 2 cut(s) 673, 705
AluBI AGCT 3 cut(s) 466, 715, 725
AluI AGCT 3 cut(s) 466, 715, 725
Alw21I GWGCWC 2 cut(s) 74, 468
Alw26I GTCTC 1 cut(s) 649
AlwNI CAGNNNCTG 1 cut(s) 564
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 4 cut(s) 251, 507, 634, 663
ApeKI GCWGC 2 cut(s) 265, 293
ApoI RAATTY 3 cut(s) 184, 308, 554
ArsI GACNNNNNNTTYG 2 cut(s) 70, 102
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 46, 701
AsuHPI GGTGA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 2 cut(s) 46, 701
BalI TGGCCA 1 cut(s) 253
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 74, 468
BbvI GCAGC 2 cut(s) 277, 280
BccI CCATC 2 cut(s) 634, 767
BcgI CGANNNNNNTGC 4 cut(s) 412, 446, 698, 732
BciT130I CCWGG 1 cut(s) 154
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 1 cut(s) 649
BfaI CTAG 5 cut(s) 21, 461, 722, 744, 798
BfmI CTRYAG 2 cut(s) 263, 676
BglII AGATCT 2 cut(s) 379, 476
BisI GCNGC 4 cut(s) 266, 294, 648, 663
BlpI GCTNAGC 2 cut(s) 223, 726
BlsI GCNGC 4 cut(s) 267, 295, 649, 664
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 2 cut(s) 46, 701
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 46, 701
BmiI GGNNCC 2 cut(s) 48, 437
BmrFI CCNGG 1 cut(s) 154
BmrI ACTGGG 1 cut(s) 266
BmsI GCATC 1 cut(s) 739
BmuI ACTGGG 1 cut(s) 266
Bpu1102I GCTNAGC 2 cut(s) 223, 726
BpuEI CTTGAG 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 153, 803
BsaXI ACNNNNNCTCC 4 cut(s) 334, 364, 673, 703
Bsc4I CCNNNNNNNGG 3 cut(s) 632, 698, 809
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 261, 349
Bse3DI GCAATG 1 cut(s) 269
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 2 cut(s) 153, 803
BseGI GGATG 2 cut(s) 456, 619
BseLI CCNNNNNNNGG 3 cut(s) 632, 698, 809
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 4 cut(s) 206, 237, 642, 717
BseNI ACTGG 2 cut(s) 261, 349
BseRI GAGGAG 3 cut(s) 101, 108, 777
BseXI GCAGC 2 cut(s) 277, 280
BshFI GGCC 4 cut(s) 253, 509, 636, 665
BsiHKAI GWGCWC 2 cut(s) 74, 468
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 3 cut(s) 632, 698, 809
BsmAI GTCTC 1 cut(s) 649
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 4 cut(s) 253, 509, 636, 665
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 74, 468
Bsp143I GATC 3 cut(s) 379, 476, 499
Bsp1720I GCTNAGC 2 cut(s) 223, 726
BspACI CCGC 3 cut(s) 623, 647, 662
BspANI GGCC 4 cut(s) 253, 509, 636, 665
BspCNI CTCAG 4 cut(s) 205, 236, 643, 718
BspLI GGNNCC 2 cut(s) 48, 437
BspMAI CTGCAG 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 2 cut(s) 261, 349
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 153, 803
BssMI GATC 3 cut(s) 379, 476, 499
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 69, 593, 807
BstAPI GCANNNNNTGC 1 cut(s) 271
BstC8I GCNNGC 4 cut(s) 291, 511, 717, 741
BstDEI CTNAG 5 cut(s) 192, 223, 482, 651, 726
BstDSI CCRYGG 1 cut(s) 803
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 2 cut(s) 456, 619
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 3 cut(s) 382, 479, 502
BstMAI GTCTC 1 cut(s) 649
BstMBI GATC 3 cut(s) 379, 476, 499
BstMWI GCNNNNNNNGC 3 cut(s) 233, 271, 736
BstNI CCWGG 1 cut(s) 154
BstNSI RCATGY 2 cut(s) 278, 859
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 152
BstSFI CTRYAG 2 cut(s) 263, 676
BstV1I GCAGC 2 cut(s) 277, 280
BstX2I RGATCY 2 cut(s) 379, 476
BstYI RGATCY 2 cut(s) 379, 476
BsuRI GGCC 4 cut(s) 253, 509, 636, 665
BtgI CCRYGG 1 cut(s) 803
BtsCI GGATG 2 cut(s) 456, 619
BtsIMutI CAGTG 3 cut(s) 74, 254, 342
Cac8I GCNNGC 4 cut(s) 291, 511, 717, 741
CaiI CAGNNNCTG 1 cut(s) 564
CfoI GCGC 1 cut(s) 615
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 2 cut(s) 46, 701
Csp6I GTAC 1 cut(s) 594
CviAII CATG 6 cut(s) 275, 305, 413, 821, 852, 856
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 5 cut(s) 192, 223, 482, 651, 726
DpnI GATC 3 cut(s) 381, 478, 501
DpnII GATC 3 cut(s) 379, 476, 499
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 3 cut(s) 251, 634, 663
Eam1105I GACNNNNNGTC 1 cut(s) 391
EciI GGCGGA 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 46, 701
Eco53kI GAGCTC 1 cut(s) 466
Eco88I CYCGRG 1 cut(s) 431
Eco91I GGTNACC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 152
EcoT22I ATGCAT 1 cut(s) 857
EcoT38I GRGCYC 1 cut(s) 468
FaeI CATG 6 cut(s) 278, 308, 416, 824, 855, 859
FaiI YATR 8 cut(s) 276, 306, 414, 443, 580, 822, 853, 857
FaqI GGGAC 1 cut(s) 273
FatI CATG 6 cut(s) 274, 304, 412, 820, 851, 855
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 4 cut(s) 266, 294, 648, 663
FokI GGATG 2 cut(s) 443, 606
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 4 cut(s) 266, 294, 648, 663
FspBI CTAG 5 cut(s) 21, 461, 722, 744, 798
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 1 cut(s) 614
GluI GCNGC 4 cut(s) 266, 294, 648, 663
HaeIII GGCC 4 cut(s) 253, 509, 636, 665
HapII CCGG 1 cut(s) 510
HhaI GCGC 1 cut(s) 615
Hin1II CATG 6 cut(s) 278, 308, 416, 824, 855, 859
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 7 cut(s) 4, 40, 368, 392, 447, 561, 749
HpaII CCGG 1 cut(s) 510
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 4 cut(s) 89, 396, 803, 826
Hpy188I TCNGA 4 cut(s) 195, 379, 560, 701
Hpy188III TCNNGA 4 cut(s) 44, 383, 433, 471
Hpy8I GTNNAC 4 cut(s) 89, 396, 803, 826
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 3 cut(s) 69, 593, 807
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 4 cut(s) 210, 265, 274, 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 271, 736
HpyF3I CTNAG 5 cut(s) 192, 223, 482, 651, 726
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 6 cut(s) 278, 308, 416, 824, 855, 859
HspAI GCGC 1 cut(s) 613
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 3 cut(s) 379, 476, 499
LmnI GCTCC 4 cut(s) 420, 435, 587, 790
Lsp1109I GCAGC 2 cut(s) 277, 280
LweI GCATC 1 cut(s) 739
MaeI CTAG 5 cut(s) 21, 461, 722, 744, 798
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 381, 478, 501
MboI GATC 3 cut(s) 379, 476, 499
MboII GAAGA 3 cut(s) 486, 501, 535
MflI RGATCY 2 cut(s) 379, 476
MhlI GDGCHC 2 cut(s) 74, 468
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 4 cut(s) 184, 308, 554, 693
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 3 cut(s) 49, 401, 555
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 9 cut(s) 24, 122, 129, 201, 370, 382, 479, 699, 798
Mox20I TGGCCA 1 cut(s) 253
Mph1103I ATGCAT 1 cut(s) 857
MroNI GCCGGC 1 cut(s) 509
MscI TGGCCA 1 cut(s) 253
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 856
Msp20I TGGCCA 1 cut(s) 253
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 154
MvaI CCWGG 1 cut(s) 154
MwoI GCNNNNNNNGC 3 cut(s) 233, 271, 736
NaeI GCCGGC 1 cut(s) 511
NdeII GATC 3 cut(s) 379, 476, 499
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 6 cut(s) 278, 308, 416, 824, 855, 859
NlaIV GGNNCC 2 cut(s) 48, 437
NmeAIII GCCGAG 1 cut(s) 612
NsbI TGCGCA 1 cut(s) 614
NsiI ATGCAT 1 cut(s) 857
NspI RCATGY 2 cut(s) 278, 859
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PdiI GCCGGC 1 cut(s) 511
PfeI GAWTC 4 cut(s) 4, 368, 447, 749
PflMI CCANNNNNTGG 1 cut(s) 809
PkrI GCNGC 4 cut(s) 267, 295, 649, 664
PleI GAGTC 3 cut(s) 48, 400, 555
PpsI GAGTC 3 cut(s) 48, 400, 555
PpuMI RGGWCCY 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 152
PspEI GGTNACC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 48, 437
PspPI GGNCC 2 cut(s) 46, 701
PspPPI RGGWCCY 1 cut(s) 46
PstI CTGCAG 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 564
PsuI RGATCY 2 cut(s) 379, 476
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 856
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 1 cut(s) 824
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 4 cut(s) 266, 294, 648, 663
Sau3AI GATC 3 cut(s) 379, 476, 499
Sau96I GGNCC 2 cut(s) 46, 701
SchI GAGTC 3 cut(s) 49, 401, 555
ScrFI CCNGG 1 cut(s) 154
SduI GDGCHC 2 cut(s) 74, 468
SetI ASST 6 cut(s) 193, 468, 612, 717, 727, 820
SfaNI GCATC 1 cut(s) 739
SfcI CTRYAG 2 cut(s) 263, 676
SinI GGWCC 2 cut(s) 46, 701
SmiMI CAYNNNNRTG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 4 cut(s) 184, 308, 554, 693
SsiI CCGC 3 cut(s) 623, 647, 662
SspI AATATT 1 cut(s) 167
SspMI CTAG 5 cut(s) 21, 461, 722, 744, 798
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 152
TaaI ACNGT 3 cut(s) 69, 593, 807
TaiI ACGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 825
TasI AATT 4 cut(s) 184, 308, 554, 693
TatI WGTACW 1 cut(s) 593
TauI GCSGC 2 cut(s) 650, 665
TfiI GAWTC 4 cut(s) 4, 368, 447, 749
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 3 cut(s) 74, 261, 349
TseI GCWGC 2 cut(s) 265, 293
TspDTI ATGAA 4 cut(s) 321, 542, 684, 840
TspRI CASTG 3 cut(s) 74, 261, 349
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 46, 701
XapI RAATTY 3 cut(s) 184, 308, 554
XceI RCATGY 2 cut(s) 278, 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 5 cut(s) 21, 461, 722, 744, 798
Zsp2I ATGCAT 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.