Rh3DG153500

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001568
Physical Location & Seq
Forward (+)
13198345 .. 13199228
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG153500.1

Sequence Viewer

Length: 660 bp
ATGGCTAATCAGGATGAAGGGTGGCCTTTGGGATTGCGACCGCTTAATGCAAGAGTTGGGTTGATGAGAAGCCGAGATTTCTCTGCCGGTTCGGCTTCGTTTAGTACTTTGGTCACTGGCTCTCCTACTTTTACTGCTTCTTCAGATCTTGATACAGAGTCAACTGGGTCTTCATTCCATGACAATGGCATCACACTAGGGAGCCTCATTGGAGTATCTAGCATTTTCGACCTCTCTCGAAGATCCAGCATGGGAAGAAGGACAATAGAAGCCTCGAAAAACAAGAAGAGTTGCAAATACAAGCATTGGTTGTTTTCACTCTGCTTTAAGCTGACCCCTGAAGCTGTGGATACGAGTAATACTCCTTCTCTAGGTCACTTTCTTGAGGCAGAAAGAAGAGGAAGTACCAACATTAACAGAAGAAATCACAGTCCAAGACCAATCGAATATGGACCAGACGATTTCTCACCTGCTGTGGCGATTTCAGACGCCAACAGCCTATTTGTGGATGGTGAAGTTGCAGCTGCTCAGGCTTCAGGTGCTTCAGAAAGTGCAGATAATGGTGGAAAAAGATCTAACAATAGAGAGTTAATAGAATATGGAAACGGACTCAGAACCCCATTGCTGGTTTCATGTTTGTGTGGACAACTCATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

23.51

Weight (kDa)

7.7

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 478
AciI CCGC 1 cut(s) 41
AclWI GGATC 1 cut(s) 237
AcuI CTGAAG 4 cut(s) 126, 360, 519, 528
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 2 cut(s) 106, 406
AfiI CCNNNNNNNGG 3 cut(s) 371, 505, 625
AluBI AGCT 3 cut(s) 331, 344, 524
AluI AGCT 3 cut(s) 331, 344, 524
AlwI GGATC 1 cut(s) 237
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 2 cut(s) 521, 524
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 459, 524
AvaII GGWCC 1 cut(s) 452
BarI GAAGNNNNNNTAC 4 cut(s) 349, 381, 388, 420
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 511, 533
BccI CCATC 1 cut(s) 503
BciVI GTATCC 1 cut(s) 343
BfaI CTAG 3 cut(s) 197, 219, 371
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 2 cut(s) 145, 572
BisI GCNGC 2 cut(s) 522, 525
BlsI GCNGC 2 cut(s) 523, 526
BmcAI AGTACT 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 203
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 505, 625
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 121, 169
Bse3DI GCAATG 1 cut(s) 620
BseGI GGATG 2 cut(s) 19, 514
BseLI CCNNNNNNNGG 3 cut(s) 371, 505, 625
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 542, 625
BseNI ACTGG 2 cut(s) 121, 169
BseXI GCAGC 2 cut(s) 511, 533
BsgI GTGCAG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 41
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 3 cut(s) 371, 505, 625
BsnI GGCC 1 cut(s) 25
Bsp143I GATC 3 cut(s) 145, 242, 572
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 2 cut(s) 541, 624
BspLI GGNNCC 1 cut(s) 203
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 2 cut(s) 121, 169
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 3 cut(s) 145, 242, 572
BssNI GRCGYC 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 431
Bst6I CTCTTC 2 cut(s) 281, 391
BstACI GRCGYC 1 cut(s) 489
BstDEI CTNAG 2 cut(s) 528, 611
BstENI CCTNNNNNAGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 19, 514
BstKTI GATC 3 cut(s) 148, 245, 575
BstMBI GATC 3 cut(s) 145, 242, 572
BstMCI CGRYCG 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 92, 530, 539
BstV1I GCAGC 2 cut(s) 511, 533
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 3 cut(s) 145, 242, 572
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 3 cut(s) 145, 242, 572
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 19, 514
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 452
CseI GACGC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 105, 405
CviAII CATG 3 cut(s) 179, 250, 633
CviQI GTAC 2 cut(s) 105, 405
DdeI CTNAG 2 cut(s) 528, 611
DpnI GATC 3 cut(s) 147, 244, 574
DpnII GATC 3 cut(s) 145, 242, 572
Eam1104I CTCTTC 2 cut(s) 281, 391
EarI CTCTTC 2 cut(s) 281, 391
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 4 cut(s) 126, 360, 519, 528
EcoNI CCTNNNNNAGG 1 cut(s) 369
FaeI CATG 3 cut(s) 182, 253, 636
FaiI YATR 5 cut(s) 180, 251, 450, 600, 634
FatI CATG 3 cut(s) 178, 249, 632
Fnu4HI GCNGC 2 cut(s) 522, 525
FokI GGATG 2 cut(s) 26, 521
Fsp4HI GCNGC 2 cut(s) 522, 525
FspBI CTAG 3 cut(s) 197, 219, 371
GluI GCNGC 2 cut(s) 522, 525
HaeIII GGCC 1 cut(s) 25
HapII CCGG 1 cut(s) 87
HgaI GACGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 3 cut(s) 182, 253, 636
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 2 cut(s) 158, 609
HpaII CCGG 1 cut(s) 87
HphI GGTGA 2 cut(s) 459, 524
Hpy166II GTNNAC 2 cut(s) 162, 644
Hpy188I TCNGA 4 cut(s) 145, 487, 547, 614
Hpy188III TCNNGA 4 cut(s) 11, 149, 237, 383
Hpy8I GTNNAC 2 cut(s) 162, 644
HpyAV CCTTC 3 cut(s) 11, 252, 375
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4V TGCA 4 cut(s) 50, 294, 521, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 530, 539
HpyF3I CTNAG 2 cut(s) 528, 611
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 3 cut(s) 182, 253, 636
Kzo9I GATC 3 cut(s) 145, 242, 572
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 2 cut(s) 511, 533
LweI GCATC 1 cut(s) 198
MaeI CTAG 3 cut(s) 197, 219, 371
MaeIII GTNAC 2 cut(s) 112, 374
MalI GATC 3 cut(s) 147, 244, 574
MboI GATC 3 cut(s) 145, 242, 572
MboII GAAGA 7 cut(s) 132, 162, 252, 267, 298, 408, 432
MflI RGATCY 3 cut(s) 145, 242, 572
MlyI GAGTC 2 cut(s) 167, 603
MnlI CCTC 5 cut(s) 215, 242, 283, 379, 392
MseI TTAA 5 cut(s) 45, 327, 414, 590, 654
MslI CAYNNNNRTG 2 cut(s) 183, 637
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 92, 530, 539
NdeII GATC 3 cut(s) 145, 242, 572
NlaIII CATG 3 cut(s) 182, 253, 636
NlaIV GGNNCC 1 cut(s) 203
NmeAIII GCCGAG 1 cut(s) 98
NmuCI GTSAC 2 cut(s) 112, 374
PaqCI CACCTGC 1 cut(s) 478
PkrI GCNGC 2 cut(s) 523, 526
PleI GAGTC 2 cut(s) 166, 603
PpsI GAGTC 2 cut(s) 166, 603
PspN4I GGNNCC 1 cut(s) 203
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 3 cut(s) 145, 242, 572
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 2 cut(s) 106, 406
RsaNI GTAC 2 cut(s) 105, 405
RseI CAYNNNNRTG 2 cut(s) 183, 637
SaqAI TTAA 5 cut(s) 45, 327, 414, 590, 654
SatI GCNGC 2 cut(s) 522, 525
Sau3AI GATC 3 cut(s) 145, 242, 572
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 2 cut(s) 167, 603
SetI ASST 7 cut(s) 234, 333, 346, 376, 472, 526, 541
SfaNI GCATC 1 cut(s) 198
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 183, 637
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SsiI CCGC 1 cut(s) 41
SspMI CTAG 3 cut(s) 197, 219, 371
TaaI ACNGT 1 cut(s) 431
TaqI TCGA 4 cut(s) 228, 238, 275, 444
TatI WGTACW 1 cut(s) 104
Tru1I TTAA 5 cut(s) 45, 327, 414, 590, 654
Tru9I TTAA 5 cut(s) 45, 327, 414, 590, 654
TscAI CASTG 1 cut(s) 121
TseFI GTSAC 2 cut(s) 112, 374
TseI GCWGC 2 cut(s) 521, 524
Tsp45I GTSAC 2 cut(s) 112, 374
TspDTI ATGAA 3 cut(s) 30, 162, 621
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 369
XspI CTAG 3 cut(s) 197, 219, 371
ZrmI AGTACT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.