Rroxscaffold_6G00414130

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001568
Physical Location & Seq
Reverse (-)
36445644 .. 36447145
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414130.1

Sequence Viewer

Length: 654 bp
ATGGCTAATCAGGATGAAGGGTGGCCTTTGGGATTGCGACCGCTTAATGCAAGAGTTGGGTTGATGAGAAGCCGAGATTTCTCTGCCGGTTCGGCTTCATTTAGTACTTTGGTCACTGGCTCTCCTACTTTTACTGCTTCTTCAGATCTTGATACAGAGTCAACTGGGTCTTCATTCCATGACAATGGCATCACACTAGGGAGCCTCATTGGAGTATCTAGCATTTTCGACCTCTCTCGAAGATCCAGCATGGGAAGAAGGACAATAGAAGCCTCGAAAAACAAGAAGAGTTGCAAATACAAGCATTGGTTGTTTTCACTCTGCTTTAAGCTGACCCCTGATGCTGTGGATACGAGTAATACTCCTTCTCTAGGTCACTTTCTTGAGGCAGAAAGAAGAGGAAGTACCAACATTAACAGAAGAAATCAGAGTCCAAGACTAATCGAATATGGACCTGACGATTTCTCACCTGCTGCGGCGATTTCAGACGCCAACAGCCCATTTGTGGATGGTGTAGTTGCAGCTGCTCAGGCTTCAGGTGCTTCAGAAAGTGCAGATAATGGTGGAACAAGATCTAACAATAGAGTTAATAGAATATGGAAACGGATTCGGAACCCCATTGCTGGTTTCATGTTTGTGTGGACAACTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.51

Weight (kDa)

9.55

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 478
AciI CCGC 2 cut(s) 41, 476
AclWI GGATC 1 cut(s) 237
AcuI CTGAAG 3 cut(s) 126, 519, 528
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 2 cut(s) 106, 406
AfiI CCNNNNNNNGG 3 cut(s) 371, 505, 623
AluBI AGCT 2 cut(s) 331, 524
AluI AGCT 2 cut(s) 331, 524
AlwI GGATC 1 cut(s) 237
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 3 cut(s) 473, 521, 524
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 459
AvaII GGWCC 1 cut(s) 452
BarI GAAGNNNNNNTAC 4 cut(s) 349, 381, 388, 420
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 460, 511, 533
BccI CCATC 1 cut(s) 503
BciVI GTATCC 1 cut(s) 343
BfaI CTAG 3 cut(s) 197, 219, 371
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 343
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 2 cut(s) 145, 572
BisI GCNGC 4 cut(s) 474, 477, 522, 525
BlsI GCNGC 4 cut(s) 475, 478, 523, 526
BmcAI AGTACT 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 2 cut(s) 203, 614
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 198, 331
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 505, 623
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 121, 169
Bse3DI GCAATG 1 cut(s) 618
BseGI GGATG 2 cut(s) 19, 514
BseLI CCNNNNNNNGG 3 cut(s) 371, 505, 623
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 2 cut(s) 121, 169
BseXI GCAGC 3 cut(s) 460, 511, 533
BsgI GTGCAG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 41
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 3 cut(s) 371, 505, 623
BsnI GGCC 1 cut(s) 25
Bsp143I GATC 3 cut(s) 145, 242, 572
BspACI CCGC 2 cut(s) 41, 476
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 203, 614
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 618
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 2 cut(s) 121, 169
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 3 cut(s) 145, 242, 572
BssNI GRCGYC 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 281, 391
BstACI GRCGYC 1 cut(s) 489
BstDEI CTNAG 1 cut(s) 528
BstENI CCTNNNNNAGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 19, 514
BstKTI GATC 3 cut(s) 148, 245, 575
BstMBI GATC 3 cut(s) 145, 242, 572
BstMCI CGRYCG 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 92, 530, 539
BstV1I GCAGC 3 cut(s) 460, 511, 533
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 3 cut(s) 145, 242, 572
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 3 cut(s) 145, 242, 572
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 19, 514
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 452
CseI GACGC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 105, 405
CviAII CATG 3 cut(s) 179, 250, 631
CviQI GTAC 2 cut(s) 105, 405
DdeI CTNAG 1 cut(s) 528
DpnI GATC 3 cut(s) 147, 244, 574
DpnII GATC 3 cut(s) 145, 242, 572
Eam1104I CTCTTC 2 cut(s) 281, 391
EarI CTCTTC 2 cut(s) 281, 391
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 3 cut(s) 126, 519, 528
EcoNI CCTNNNNNAGG 1 cut(s) 369
FaeI CATG 3 cut(s) 182, 253, 634
FaiI YATR 5 cut(s) 180, 251, 450, 598, 632
FatI CATG 3 cut(s) 178, 249, 630
Fnu4HI GCNGC 4 cut(s) 474, 477, 522, 525
FokI GGATG 2 cut(s) 26, 521
Fsp4HI GCNGC 4 cut(s) 474, 477, 522, 525
FspBI CTAG 3 cut(s) 197, 219, 371
GluI GCNGC 4 cut(s) 474, 477, 522, 525
HaeIII GGCC 1 cut(s) 25
HapII CCGG 1 cut(s) 87
HgaI GACGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 3 cut(s) 182, 253, 634
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 3 cut(s) 158, 430, 607
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 459
Hpy166II GTNNAC 2 cut(s) 162, 642
Hpy188I TCNGA 5 cut(s) 145, 429, 487, 547, 612
Hpy188III TCNNGA 4 cut(s) 11, 149, 237, 383
Hpy8I GTNNAC 2 cut(s) 162, 642
HpyAV CCTTC 3 cut(s) 11, 252, 375
HpyCH4V TGCA 4 cut(s) 50, 294, 521, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 530, 539
HpyF3I CTNAG 1 cut(s) 528
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 3 cut(s) 182, 253, 634
Kzo9I GATC 3 cut(s) 145, 242, 572
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 3 cut(s) 460, 511, 533
LweI GCATC 2 cut(s) 198, 331
MaeI CTAG 3 cut(s) 197, 219, 371
MaeIII GTNAC 2 cut(s) 112, 374
MalI GATC 3 cut(s) 147, 244, 574
MboI GATC 3 cut(s) 145, 242, 572
MboII GAAGA 7 cut(s) 132, 162, 252, 267, 298, 408, 432
MflI RGATCY 3 cut(s) 145, 242, 572
MlyI GAGTC 2 cut(s) 167, 439
MnlI CCTC 5 cut(s) 215, 242, 283, 379, 392
MseI TTAA 5 cut(s) 45, 327, 414, 588, 652
MslI CAYNNNNRTG 2 cut(s) 183, 635
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 92, 530, 539
NdeII GATC 3 cut(s) 145, 242, 572
NlaIII CATG 3 cut(s) 182, 253, 634
NlaIV GGNNCC 2 cut(s) 203, 614
NmeAIII GCCGAG 1 cut(s) 98
NmuCI GTSAC 2 cut(s) 112, 374
PaqCI CACCTGC 1 cut(s) 478
PfeI GAWTC 1 cut(s) 607
PkrI GCNGC 4 cut(s) 475, 478, 523, 526
PleI GAGTC 2 cut(s) 166, 438
PpsI GAGTC 2 cut(s) 166, 438
PspN4I GGNNCC 2 cut(s) 203, 614
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 3 cut(s) 145, 242, 572
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 2 cut(s) 106, 406
RsaNI GTAC 2 cut(s) 105, 405
RseI CAYNNNNRTG 2 cut(s) 183, 635
SaqAI TTAA 5 cut(s) 45, 327, 414, 588, 652
SatI GCNGC 4 cut(s) 474, 477, 522, 525
Sau3AI GATC 3 cut(s) 145, 242, 572
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 2 cut(s) 167, 439
SetI ASST 7 cut(s) 234, 333, 376, 457, 472, 526, 541
SfaNI GCATC 2 cut(s) 198, 331
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 183, 635
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SsiI CCGC 2 cut(s) 41, 476
SspMI CTAG 3 cut(s) 197, 219, 371
TaqI TCGA 4 cut(s) 228, 238, 275, 444
TatI WGTACW 1 cut(s) 104
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 5 cut(s) 45, 327, 414, 588, 652
Tru9I TTAA 5 cut(s) 45, 327, 414, 588, 652
TscAI CASTG 1 cut(s) 121
TseFI GTSAC 2 cut(s) 112, 374
TseI GCWGC 3 cut(s) 473, 521, 524
Tsp45I GTSAC 2 cut(s) 112, 374
TspDTI ATGAA 4 cut(s) 30, 87, 162, 619
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 369
XspI CTAG 3 cut(s) 197, 219, 371
ZrmI AGTACT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.