Rh2DG438800

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001568
Physical Location & Seq
Reverse (-)
63570503 .. 63584511
14009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG438800.1

Sequence Viewer

Length: 279 bp
ATGTGGGAGGAAGGCTGGCCTTTGGGTTTGCAGCCAATGCATGTGAGAGTTGGGCTGGCTGCTCGAGACCGTGAAGTTTCTGGATCGGTGTCATTCAATACTTTACTAACTGGCTCTCCGACCTCCTCCACAGACTCTTCTTCGGATCTGGATACAGATGTCAGTGCTGTAAATTACAGAGATAATGGCACCTTCGACTCGAAAAGCTTCCTCTCAAGCACTAGCAATGGCAAAAAGAATGAGGGAAATCCAGCTGAGAAGGATAAGCAAATCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.97

Weight (kDa)

4.75

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AclWI GGATC 2 cut(s) 91, 153
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 2 cut(s) 207, 254
AluI AGCT 2 cut(s) 207, 254
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 2 cut(s) 91, 153
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 31, 59
Asp700I GAANNNNTTC 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 63
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 43, 46
BciVI GTATCC 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 145
BisI GCNGC 2 cut(s) 32, 60
BlsI GCNGC 2 cut(s) 33, 61
BmeT110I CYCGRG 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bse1I ACTGG 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 232
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 43, 46
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 63
BsmAI GTCTC 1 cut(s) 60
BsnI GGCC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 63
Bsp143I GATC 2 cut(s) 83, 145
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 2 cut(s) 91, 153
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 1 cut(s) 115
BssMI GATC 2 cut(s) 83, 145
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 17, 57
BstDEI CTNAG 1 cut(s) 255
BstKTI GATC 2 cut(s) 86, 148
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 2 cut(s) 83, 145
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNSI RCATGY 1 cut(s) 44
BstV1I GCAGC 2 cut(s) 43, 46
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 17, 57
CviAII CATG 1 cut(s) 41
CviJI RGCY 8 cut(s) 15, 19, 34, 55, 59, 114, 207, 254
CviKI_1 RGCY 8 cut(s) 15, 19, 34, 55, 59, 114, 207, 254
DdeI CTNAG 1 cut(s) 255
DpnI GATC 2 cut(s) 85, 147
DpnII GATC 2 cut(s) 83, 145
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 42
FaeI CATG 1 cut(s) 44
FaiI YATR 1 cut(s) 42
FatI CATG 1 cut(s) 40
Fnu4HI GCNGC 2 cut(s) 32, 60
Fsp4HI GCNGC 2 cut(s) 32, 60
FspBI CTAG 1 cut(s) 222
GluI GCNGC 2 cut(s) 32, 60
HaeIII GGCC 1 cut(s) 19
Hin1II CATG 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 2 cut(s) 134, 197
Hpy188I TCNGA 2 cut(s) 120, 145
Hpy188III TCNNGA 3 cut(s) 65, 81, 149
HpyAV CCTTC 3 cut(s) 5, 202, 253
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 2 cut(s) 31, 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 1 cut(s) 44
Kzo9I GATC 2 cut(s) 83, 145
LpnPI CCDG 5 cut(s) 41, 66, 96, 134, 264
Lsp1109I GCAGC 2 cut(s) 43, 46
MaeI CTAG 1 cut(s) 222
MalI GATC 2 cut(s) 85, 147
MboI GATC 2 cut(s) 83, 145
MboII GAAGA 2 cut(s) 129, 132
MflI RGATCY 1 cut(s) 145
MluCI AATT 1 cut(s) 172
MlyI GAGTC 2 cut(s) 128, 191
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 4 cut(s) 133, 136, 221, 235
Mph1103I ATGCAT 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeII GATC 2 cut(s) 83, 145
NlaIII CATG 1 cut(s) 44
NlaIV GGNNCC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 42
NspI RCATGY 1 cut(s) 44
PaeR7I CTCGAG 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 206
PkrI GCNGC 2 cut(s) 33, 61
PleI GAGTC 2 cut(s) 128, 191
PpsI GAGTC 2 cut(s) 128, 191
PspN4I GGNNCC 1 cut(s) 190
PsuI RGATCY 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 254
SatI GCNGC 2 cut(s) 32, 60
Sau3AI GATC 2 cut(s) 83, 145
SchI GAGTC 2 cut(s) 128, 191
SetI ASST 4 cut(s) 125, 194, 209, 256
Sfr274I CTCGAG 1 cut(s) 63
SlaI CTCGAG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 63, 214
SmoI CTYRAG 2 cut(s) 63, 214
Sse9I AATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 3 cut(s) 64, 195, 200
TasI AATT 1 cut(s) 172
TscAI CASTG 1 cut(s) 169
TseI GCWGC 2 cut(s) 31, 59
TspRI CASTG 1 cut(s) 169
XceI RCATGY 1 cut(s) 44
XhoI CTCGAG 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 1 cut(s) 222
Zsp2I ATGCAT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.