Rmu_ssc0000170.1_g000006

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000170.1
OG0001568
Physical Location & Seq
Forward (+)
11676 .. 13730
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000170.1_g000006.1.cds

Sequence Viewer

Length: 435 bp
atggctcgtgagcaggaggaaggctggcctctgggtttgcagccaatgcatgtgagagttgggctggctgctcgagaccgtgaagtttctggatcggtgtcattcaatactttactaactggctctccgacctcctccacagactcttcttcggatctggatacagaggtcaatggtgagaatgtgaacagtactaaaactcctccatccctcggccatttccttgccgaagagagaagagctgccattgatgaatacagaagaaaccctcaaatttatggcccagatgatgagcttgccatggctcagcaggctgctacagaacccaactccctctttgttaatggccaagtagctcctcctcctcaatctagtccatggtttgattcagatgttgacagaagaaaaccattgctattttcatgcatgtgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

15.78

Weight (kDa)

4.58

Isoelectric Point (pI)

64.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 100, 162
AcoI YGGCCR 2 cut(s) 214, 346
AcsI RAATTY 1 cut(s) 273
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 1 cut(s) 106
AluBI AGCT 3 cut(s) 242, 295, 356
AluI AGCT 3 cut(s) 242, 295, 356
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 2 cut(s) 100, 162
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 4 cut(s) 26, 214, 280, 346
ApeKI GCWGC 4 cut(s) 40, 68, 242, 314
ApoI RAATTY 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 72
BaeI ACNNNNGTAYC 2 cut(s) 153, 186
BalI TGGCCA 1 cut(s) 348
BauI CACGAG 1 cut(s) 6
BbvI GCAGC 4 cut(s) 52, 55, 229, 301
BccI CCATC 1 cut(s) 214
BciVI GTATCC 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 318
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 4 cut(s) 41, 69, 243, 315
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 4 cut(s) 42, 70, 244, 316
BmcAI AGTACT 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 281
Bpu1102I GCTNAGC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 211, 300, 377
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 184, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 410
BseDI CCNNGG 3 cut(s) 211, 300, 377
BseGI GGATG 1 cut(s) 206
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 5 cut(s) 124, 192, 348, 351, 354
BseXI GCAGC 4 cut(s) 52, 55, 229, 301
BshFI GGCC 4 cut(s) 28, 216, 282, 348
BsiHKCI CYCGRG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 69
BsnI GGCC 4 cut(s) 28, 216, 282, 348
Bso31I GGTCTC 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 72
Bsp143I GATC 2 cut(s) 92, 154
Bsp1720I GCTNAGC 1 cut(s) 306
Bsp19I CCATGG 2 cut(s) 300, 377
BspANI GGCC 4 cut(s) 28, 216, 282, 348
BspCNI CTCAG 1 cut(s) 319
BspPI GGATC 2 cut(s) 100, 162
BspQI GCTCTTC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 410
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 211, 300, 377
BssMI GATC 2 cut(s) 92, 154
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 300, 377
Bst2BI CACGAG 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 80, 191
Bst6I CTCTTC 3 cut(s) 151, 225, 232
BstAPI GCANNNNNTGC 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 26, 66, 297, 312
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 2 cut(s) 300, 377
BstF5I GGATG 1 cut(s) 206
BstKTI GATC 2 cut(s) 95, 157
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 92, 154
BstMWI GCNNNNNNNGC 2 cut(s) 46, 311
BstNSI RCATGY 2 cut(s) 53, 430
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 4 cut(s) 52, 55, 229, 301
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 4 cut(s) 28, 216, 282, 348
BtgI CCRYGG 2 cut(s) 300, 377
BtsCI GGATG 1 cut(s) 206
Cac8I GCNNGC 4 cut(s) 26, 66, 297, 312
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 192
CviAII CATG 5 cut(s) 50, 301, 378, 423, 427
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 94, 156
DpnII GATC 2 cut(s) 92, 154
EaeI YGGCCR 2 cut(s) 214, 346
Eam1104I CTCTTC 3 cut(s) 151, 225, 232
EarI CTCTTC 3 cut(s) 151, 225, 232
Eco130I CCWWGG 2 cut(s) 300, 377
Eco31I GGTCTC 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 300, 377
EcoT22I ATGCAT 2 cut(s) 51, 428
ErhI CCWWGG 2 cut(s) 300, 377
FaeI CATG 5 cut(s) 53, 304, 381, 426, 430
FaiI YATR 6 cut(s) 51, 279, 302, 379, 424, 428
FatI CATG 5 cut(s) 49, 300, 377, 422, 426
Fnu4HI GCNGC 4 cut(s) 41, 69, 243, 315
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 41, 69, 243, 315
FspBI CTAG 1 cut(s) 372
GluI GCNGC 4 cut(s) 41, 69, 243, 315
HaeIII GGCC 4 cut(s) 28, 216, 282, 348
Hin1II CATG 5 cut(s) 53, 304, 381, 426, 430
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 143, 386
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 187, 397
Hpy188I TCNGA 3 cut(s) 129, 154, 391
Hpy188III TCNNGA 4 cut(s) 8, 74, 90, 158
Hpy8I GTNNAC 2 cut(s) 187, 397
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 2 cut(s) 80, 191
HpyCH4V TGCA 3 cut(s) 40, 49, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 311
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 5 cut(s) 53, 304, 381, 426, 430
Kzo9I GATC 2 cut(s) 92, 154
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 8 cut(s) 10, 17, 50, 75, 105, 143, 296, 297
Lsp1109I GCAGC 4 cut(s) 52, 55, 229, 301
MaeI CTAG 1 cut(s) 372
MalI GATC 2 cut(s) 94, 156
MboI GATC 2 cut(s) 92, 154
MboII GAAGA 6 cut(s) 138, 141, 242, 249, 273, 414
MflI RGATCY 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 152
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 2 cut(s) 51, 428
MscI TGGCCA 1 cut(s) 348
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 427
Msp20I TGGCCA 1 cut(s) 348
MwoI GCNNNNNNNGC 2 cut(s) 46, 311
NcoI CCATGG 2 cut(s) 300, 377
NdeII GATC 2 cut(s) 92, 154
NlaIII CATG 5 cut(s) 53, 304, 381, 426, 430
NmeAIII GCCGAG 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 51, 428
NspI RCATGY 2 cut(s) 53, 430
PaeR7I CTCGAG 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 4 cut(s) 42, 70, 244, 316
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 281
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 41, 69, 243, 315
Sau3AI GATC 2 cut(s) 92, 154
Sau96I GGNCC 1 cut(s) 281
ScaI AGTACT 1 cut(s) 193
SchI GAGTC 1 cut(s) 137
SetI ASST 5 cut(s) 134, 171, 244, 297, 358
SfcI CTRYAG 1 cut(s) 318
Sfr274I CTCGAG 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 1 cut(s) 273
SspMI CTAG 1 cut(s) 372
StyI CCWWGG 2 cut(s) 300, 377
TaaI ACNGT 2 cut(s) 80, 191
TaqI TCGA 1 cut(s) 73
TasI AATT 1 cut(s) 273
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseI GCWGC 4 cut(s) 40, 68, 242, 314
TspDTI ATGAA 2 cut(s) 267, 411
XapI RAATTY 1 cut(s) 273
XceI RCATGY 2 cut(s) 53, 430
XhoI CTCGAG 1 cut(s) 72
XspI CTAG 1 cut(s) 372
ZrmI AGTACT 1 cut(s) 193
Zsp2I ATGCAT 2 cut(s) 51, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.