Rmu_sc0008017.1_g000004

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008017.1
OG0001568
Physical Location & Seq
Reverse (-)
12783 .. 13518
736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008017.1_g000004.1.cds

Sequence Viewer

Length: 597 bp
atgagaagccgagatttctctgccggttcggcttcgtttagtactttggtcactggctctcctacttttactgcttcttcagatcttgatacagagtcaactgggtcttcattccatgacaatggcatcacactagggagcctcattggagtatctagcattttcgacctctctcgaagatccagcatgggaagaaggacaatagaagcctcgaaaaacaagaagagttgcaaatacaagcattggttgttttcactctgctttaagctgacccctgaagctgtggatacgagtaatactccttctctaggtcactttcttgaggtggaaagaagaggaagtaccaacattaacagaagaaatcacagtccaagaccaatcgaatatggacctgacgatttctcacctgctgtggcgatttcagacgccaacagcctatttgtggatggtgtagttgcagctgctcaggcttcaagtgcttcagaaagtgcagataatggtggaacaagatctaacaatagagagttagtagaatatggaaacggattcagaaccccattgctggtttcatgtatgtgtggacaactcattaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.26

Weight (kDa)

7.71

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 415
Acc36I ACCTGC 1 cut(s) 415
AclWI GGATC 1 cut(s) 174
AcuI CTGAAG 3 cut(s) 63, 297, 465
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 43, 343
AfiI CCNNNNNNNGG 3 cut(s) 308, 442, 562
AgsI TTSAA 1 cut(s) 474
AluBI AGCT 3 cut(s) 268, 281, 461
AluI AGCT 3 cut(s) 268, 281, 461
AlwI GGATC 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 458, 461
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 396
AvaII GGWCC 1 cut(s) 389
BarI GAAGNNNNNNTAC 4 cut(s) 286, 318, 325, 357
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 448, 470
BccI CCATC 1 cut(s) 440
BciVI GTATCC 1 cut(s) 280
BfaI CTAG 3 cut(s) 134, 156, 308
BfuAI ACCTGC 1 cut(s) 415
BfuI GTATCC 1 cut(s) 280
BglI GCCNNNNNGGC 1 cut(s) 29
BglII AGATCT 2 cut(s) 82, 509
BisI GCNGC 2 cut(s) 459, 462
BlsI GCNGC 2 cut(s) 460, 463
BmcAI AGTACT 1 cut(s) 43
Bme18I GGWCC 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 283, 315
Bpu10I CCTNAGC 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 426
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 442, 562
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 58, 106
Bse3DI GCAATG 1 cut(s) 557
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 3 cut(s) 308, 442, 562
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 2 cut(s) 58, 106
BseXI GCAGC 2 cut(s) 448, 470
BsgI GTGCAG 1 cut(s) 510
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 308, 442, 562
Bsp143I GATC 3 cut(s) 82, 179, 509
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 140
BspMI ACCTGC 1 cut(s) 415
BspPI GGATC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 2 cut(s) 58, 106
BssAI RCCGGY 1 cut(s) 23
BssMI GATC 3 cut(s) 82, 179, 509
BssNI GRCGYC 1 cut(s) 426
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 2 cut(s) 218, 328
BstACI GRCGYC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 465
BstENI CCTNNNNNAGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 3 cut(s) 85, 182, 512
BstMBI GATC 3 cut(s) 82, 179, 509
BstMWI GCNNNNNNNGC 3 cut(s) 29, 467, 476
BstV1I GCAGC 2 cut(s) 448, 470
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 3 cut(s) 82, 179, 509
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 3 cut(s) 82, 179, 509
BsuI GTATCC 1 cut(s) 280
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 389
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 42, 342
CviAII CATG 3 cut(s) 116, 187, 570
CviQI GTAC 2 cut(s) 42, 342
DdeI CTNAG 1 cut(s) 465
DpnI GATC 3 cut(s) 84, 181, 511
DpnII GATC 3 cut(s) 82, 179, 509
Eam1104I CTCTTC 2 cut(s) 218, 328
EarI CTCTTC 2 cut(s) 218, 328
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 3 cut(s) 63, 297, 465
EcoNI CCTNNNNNAGG 1 cut(s) 306
FaeI CATG 3 cut(s) 119, 190, 573
FaiI YATR 6 cut(s) 117, 188, 387, 537, 571, 575
FatI CATG 3 cut(s) 115, 186, 569
Fnu4HI GCNGC 2 cut(s) 459, 462
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 2 cut(s) 459, 462
FspBI CTAG 3 cut(s) 134, 156, 308
GluI GCNGC 2 cut(s) 459, 462
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 3 cut(s) 119, 190, 573
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 2 cut(s) 95, 546
HpaII CCGG 1 cut(s) 24
HphI GGTGA 1 cut(s) 396
Hpy166II GTNNAC 2 cut(s) 99, 581
Hpy188I TCNGA 4 cut(s) 82, 424, 484, 551
Hpy188III TCNNGA 3 cut(s) 86, 174, 320
Hpy8I GTNNAC 2 cut(s) 99, 581
HpyAV CCTTC 2 cut(s) 189, 312
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 231, 458, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 467, 476
HpyF3I CTNAG 1 cut(s) 465
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 3 cut(s) 119, 190, 573
Kzo9I GATC 3 cut(s) 82, 179, 509
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 9 cut(s) 37, 39, 87, 196, 288, 405, 420, 452, 548
Lsp1109I GCAGC 2 cut(s) 448, 470
LweI GCATC 1 cut(s) 135
MaeI CTAG 3 cut(s) 134, 156, 308
MaeIII GTNAC 2 cut(s) 49, 311
MalI GATC 3 cut(s) 84, 181, 511
MboI GATC 3 cut(s) 82, 179, 509
MboII GAAGA 7 cut(s) 69, 99, 189, 204, 235, 345, 369
MflI RGATCY 3 cut(s) 82, 179, 509
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 5 cut(s) 152, 179, 220, 316, 329
MseI TTAA 3 cut(s) 264, 351, 591
MslI CAYNNNNRTG 2 cut(s) 120, 574
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 29, 467, 476
NdeII GATC 3 cut(s) 82, 179, 509
NlaIII CATG 3 cut(s) 119, 190, 573
NlaIV GGNNCC 1 cut(s) 140
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 2 cut(s) 49, 311
PaqCI CACCTGC 1 cut(s) 415
PfeI GAWTC 1 cut(s) 546
PkrI GCNGC 2 cut(s) 460, 463
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 1 cut(s) 389
PsuI RGATCY 3 cut(s) 82, 179, 509
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 43, 343
RsaNI GTAC 2 cut(s) 42, 342
RseI CAYNNNNRTG 2 cut(s) 120, 574
SaqAI TTAA 3 cut(s) 264, 351, 591
SatI GCNGC 2 cut(s) 459, 462
Sau3AI GATC 3 cut(s) 82, 179, 509
Sau96I GGNCC 1 cut(s) 389
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 1 cut(s) 104
SetI ASST 8 cut(s) 171, 270, 283, 313, 327, 394, 409, 463
SfaNI GCATC 1 cut(s) 135
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 2 cut(s) 120, 574
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SspMI CTAG 3 cut(s) 134, 156, 308
TaaI ACNGT 1 cut(s) 368
TaqI TCGA 4 cut(s) 165, 175, 212, 381
TatI WGTACW 1 cut(s) 41
TfiI GAWTC 1 cut(s) 546
Tru1I TTAA 3 cut(s) 264, 351, 591
Tru9I TTAA 3 cut(s) 264, 351, 591
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 2 cut(s) 49, 311
TseI GCWGC 2 cut(s) 458, 461
Tsp45I GTSAC 2 cut(s) 49, 311
TspDTI ATGAA 2 cut(s) 99, 558
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 389
XagI CCTNNNNNAGG 1 cut(s) 306
XspI CTAG 3 cut(s) 134, 156, 308
ZrmI AGTACT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.