Rroxscaffold_1G00016730

At3g17950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001568
Physical Location & Seq
Forward (+)
20667711 .. 20669935
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016730.1

Sequence Viewer

Length: 735 bp
ATGGCTCGTGAGCAGACTCTGGCTTTTCTCATCACATTGTTCAGTCCTGTGATTTCTCTAAGTGCACATGAGGAAGGCTGGCCTCTGGGTTTGCAGCCAATGCATGTGAGAGTTGGGCTGGCTGCTCGAGACCGTGAAGTTTCTGGATCGGTGTCATTCAATACTTTACTAACTGGCTCTCCGACCTCCTCCACAGACTCTTCTTCGGATCTGGACACAGAGGTATATTATATACATGGTTGGTATACAGTATTTAGTGCTACTGTGCGGTCGACAGGGTCTTTCTTCCATGATAGAAGCATCACACTTGGGAATCTTATAGGAATCAATAGCATTCTAGAGCTATCAAGGAGATCATTAAGAGGAAGAAAGACTGATCAGATCTGTAAGGACAAAAAGAGCAACAACAACAACAACAACAACAGCACCAAGTTCAGATTGTGTTGTTTCTCTCTATGCTCAAGGGACAGTACTGATGGTGAGAATGTGAACAGTACTAAAACTCCTCCATCCCTCGGCCATTTCCTTGCAGAAGAGAGAAGAGCTGCCATGGATGAATACAGTAGAAACCCTCAAATTTATGGCCCAGATGATGAGCTTGCCATGGCTCAGCAGGCTGCTACAGAACCCAACTCCCTCTTTGTTAATGGCCAAGTAGCTCCTCCTCAATCTAGTCCATGGTTTGATTCAGATGTTGACAGAAGAAAACCATTGCTATTTTCATGCATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.06

Weight (kDa)

5.96

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 245, 272
AciI CCGC 1 cut(s) 268
AclWI GGATC 2 cut(s) 154, 216
AcoI YGGCCR 2 cut(s) 517, 649
AcsI RAATTY 1 cut(s) 576
AfaI GTAC 2 cut(s) 472, 496
AfiI CCNNNNNNNGG 1 cut(s) 515
AgsI TTSAA 1 cut(s) 160
AluBI AGCT 4 cut(s) 343, 545, 598, 659
AluI AGCT 4 cut(s) 343, 545, 598, 659
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 63
AlwI GGATC 2 cut(s) 154, 216
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 4 cut(s) 80, 517, 583, 649
ApaLI GTGCAC 1 cut(s) 63
ApeKI GCWGC 4 cut(s) 94, 122, 545, 617
ApoI RAATTY 1 cut(s) 576
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 67
BalI TGGCCA 1 cut(s) 651
BauI CACGAG 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 4 cut(s) 106, 109, 532, 604
BccI CCATC 2 cut(s) 470, 517
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 2 cut(s) 338, 672
BfmI CTRYAG 1 cut(s) 621
BglII AGATCT 1 cut(s) 381
BisI GCNGC 4 cut(s) 95, 123, 546, 618
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 4 cut(s) 96, 124, 547, 619
BmcAI AGTACT 2 cut(s) 472, 496
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 584
BmsI GCATC 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 514, 549, 603, 677
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 487, 517
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 710
BseDI CCNNGG 4 cut(s) 514, 549, 603, 677
BseGI GGATG 2 cut(s) 509, 559
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 710
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 4 cut(s) 178, 495, 651, 654
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 4 cut(s) 106, 109, 532, 604
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 4 cut(s) 82, 519, 585, 651
BsiEI CGRYCG 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 4 cut(s) 82, 519, 585, 651
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 5 cut(s) 146, 208, 353, 376, 381
Bsp1720I GCTNAGC 1 cut(s) 609
Bsp19I CCATGG 3 cut(s) 549, 603, 677
BspACI CCGC 1 cut(s) 268
BspANI GGCC 4 cut(s) 82, 519, 585, 651
BspCNI CTCAG 1 cut(s) 622
BspPI GGATC 2 cut(s) 154, 216
BspQI GCTCTTC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 710
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 4 cut(s) 514, 549, 603, 677
BssMI GATC 5 cut(s) 146, 208, 353, 376, 381
BssNAI GTATAC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 3 cut(s) 549, 603, 677
Bst1107I GTATAC 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 6
Bst4CI ACNGT 6 cut(s) 134, 250, 265, 470, 494, 563
Bst6I CTCTTC 3 cut(s) 205, 528, 535
BstAPI GCANNNNNTGC 1 cut(s) 100
BstC8I GCNNGC 4 cut(s) 80, 120, 600, 615
BstDEI CTNAG 2 cut(s) 59, 609
BstDSI CCRYGG 3 cut(s) 549, 603, 677
BstF5I GGATG 2 cut(s) 509, 559
BstKTI GATC 5 cut(s) 149, 211, 356, 379, 384
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 5 cut(s) 146, 208, 353, 376, 381
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 2 cut(s) 100, 614
BstNSI RCATGY 2 cut(s) 107, 730
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 67
BstV1I GCAGC 4 cut(s) 106, 109, 532, 604
BstX2I RGATCY 2 cut(s) 208, 381
BstYI RGATCY 2 cut(s) 208, 381
BstZ17I GTATAC 1 cut(s) 246
BsuRI GGCC 4 cut(s) 82, 519, 585, 651
BtgI CCRYGG 3 cut(s) 549, 603, 677
BtsCI GGATG 2 cut(s) 509, 559
Cac8I GCNNGC 4 cut(s) 80, 120, 600, 615
CaiI CAGNNNCTG 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 2 cut(s) 471, 495
CviAII CATG 9 cut(s) 68, 104, 236, 290, 550, 604, 678, 723, 727
CviQI GTAC 2 cut(s) 471, 495
DdeI CTNAG 2 cut(s) 59, 609
DpnI GATC 5 cut(s) 148, 210, 355, 378, 383
DpnII GATC 5 cut(s) 146, 208, 353, 376, 381
EaeI YGGCCR 2 cut(s) 517, 649
Eam1104I CTCTTC 3 cut(s) 205, 528, 535
EarI CTCTTC 3 cut(s) 205, 528, 535
Eco130I CCWWGG 3 cut(s) 549, 603, 677
Eco31I GGTCTC 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 126
EcoT14I CCWWGG 3 cut(s) 549, 603, 677
EcoT22I ATGCAT 2 cut(s) 105, 728
ErhI CCWWGG 3 cut(s) 549, 603, 677
FaeI CATG 9 cut(s) 71, 107, 239, 293, 553, 607, 681, 726, 730
FaqI GGGAC 1 cut(s) 479
FatI CATG 9 cut(s) 67, 103, 235, 289, 549, 603, 677, 722, 726
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 2 cut(s) 245, 272
Fnu4HI GCNGC 4 cut(s) 95, 123, 546, 618
FokI GGATG 2 cut(s) 496, 566
Fsp4HI GCNGC 4 cut(s) 95, 123, 546, 618
FspBI CTAG 2 cut(s) 338, 672
GluI GCNGC 4 cut(s) 95, 123, 546, 618
HaeIII GGCC 4 cut(s) 82, 519, 585, 651
Hin1II CATG 9 cut(s) 71, 107, 239, 293, 553, 607, 681, 726, 730
HincII GTYRAC 2 cut(s) 273, 697
HindII GTYRAC 2 cut(s) 273, 697
HinfI GANTC 5 cut(s) 16, 197, 313, 324, 686
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 5 cut(s) 65, 246, 273, 490, 697
Hpy188I TCNGA 5 cut(s) 183, 208, 381, 437, 691
Hpy188III TCNNGA 5 cut(s) 8, 128, 144, 212, 338
Hpy8I GTNNAC 5 cut(s) 65, 246, 273, 490, 697
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 6 cut(s) 134, 250, 265, 470, 494, 563
HpyCH4V TGCA 5 cut(s) 65, 94, 103, 530, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 614
HpyF3I CTNAG 2 cut(s) 59, 609
Hsp92II CATG 9 cut(s) 71, 107, 239, 293, 553, 607, 681, 726, 730
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 5 cut(s) 146, 208, 353, 376, 381
LguI GCTCTTC 1 cut(s) 535
LmnI GCTCC 1 cut(s) 664
Lsp1109I GCAGC 4 cut(s) 106, 109, 532, 604
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 338, 672
MalI GATC 5 cut(s) 148, 210, 355, 378, 383
MboI GATC 5 cut(s) 146, 208, 353, 376, 381
MboII GAAGA 7 cut(s) 192, 195, 277, 378, 545, 552, 714
MflI RGATCY 2 cut(s) 208, 381
MhlI GDGCHC 1 cut(s) 67
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 1 cut(s) 576
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 2 cut(s) 10, 191
MmeI TCCRAC 1 cut(s) 206
Mox20I TGGCCA 1 cut(s) 651
Mph1103I ATGCAT 2 cut(s) 105, 728
MscI TGGCCA 1 cut(s) 651
MseI TTAA 2 cut(s) 359, 645
MslI CAYNNNNRTG 1 cut(s) 727
Msp20I TGGCCA 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 100, 614
NcoI CCATGG 3 cut(s) 549, 603, 677
NdeII GATC 5 cut(s) 146, 208, 353, 376, 381
NlaIII CATG 9 cut(s) 71, 107, 239, 293, 553, 607, 681, 726, 730
NmeAIII GCCGAG 1 cut(s) 495
NsiI ATGCAT 2 cut(s) 105, 728
NspI RCATGY 2 cut(s) 107, 730
PaeR7I CTCGAG 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 535
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 3 cut(s) 313, 324, 686
PflFI GACNNNGTC 1 cut(s) 277
PkrI GCNGC 4 cut(s) 96, 124, 547, 619
PleI GAGTC 2 cut(s) 10, 191
PpsI GAGTC 2 cut(s) 10, 191
PspPI GGNCC 1 cut(s) 584
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 2 cut(s) 208, 381
PsyI GACNNNGTC 1 cut(s) 277
RsaI GTAC 2 cut(s) 472, 496
RsaNI GTAC 2 cut(s) 471, 495
RseI CAYNNNNRTG 1 cut(s) 727
SalI GTCGAC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 535
SaqAI TTAA 2 cut(s) 359, 645
SatI GCNGC 4 cut(s) 95, 123, 546, 618
Sau3AI GATC 5 cut(s) 146, 208, 353, 376, 381
Sau96I GGNCC 1 cut(s) 584
ScaI AGTACT 2 cut(s) 472, 496
SchI GAGTC 2 cut(s) 10, 191
SduI GDGCHC 1 cut(s) 67
SetI ASST 6 cut(s) 188, 225, 345, 547, 600, 661
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 621
Sfr274I CTCGAG 1 cut(s) 126
SlaI CTCGAG 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 727
SmlI CTYRAG 2 cut(s) 126, 460
SmoI CTYRAG 2 cut(s) 126, 460
Sse9I AATT 1 cut(s) 576
SsiI CCGC 1 cut(s) 268
SspMI CTAG 2 cut(s) 338, 672
StyI CCWWGG 3 cut(s) 549, 603, 677
TaaI ACNGT 6 cut(s) 134, 250, 265, 470, 494, 563
TaqI TCGA 2 cut(s) 127, 272
TasI AATT 1 cut(s) 576
TatI WGTACW 2 cut(s) 470, 494
TfiI GAWTC 3 cut(s) 313, 324, 686
Tru1I TTAA 2 cut(s) 359, 645
Tru9I TTAA 2 cut(s) 359, 645
TseI GCWGC 4 cut(s) 94, 122, 545, 617
TspDTI ATGAA 2 cut(s) 570, 711
Tth111I GACNNNGTC 1 cut(s) 277
VneI GTGCAC 1 cut(s) 63
XapI RAATTY 1 cut(s) 576
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 2 cut(s) 107, 730
XhoI CTCGAG 1 cut(s) 126
XmiI GTMKAC 2 cut(s) 245, 272
XspI CTAG 2 cut(s) 338, 672
ZrmI AGTACT 2 cut(s) 472, 496
Zsp2I ATGCAT 2 cut(s) 105, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.