pycom04g18220

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002958
Physical Location & Seq
Forward (+)
20325792 .. 20326558
767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGCAGACAAGTGCCTTTGGAAAATCCAAAGGCTTGTGGAAGACTCTTACAGAGGGCAGCTGGATTGATGCATTTGATGGTCTAGGAATCTCCCTGTTGCTGACCGCGAACCCTGCAATTCAGTATACAGTGTTCGATCAGCTAAAACTCAGACTCCTGAAAAGGAAGAATAAAACGGGCAAGGATTCTTCTCCAGAAGCCCTTTCTGCCTTCACAGCATTTGTTTTAGGTGCAGTCTCAAAGACTGTTGCCACTGTTCTGACCTACCCCGCCATCAGGTGTAAGGTCATGATCCAAGCTGCTGACGAAGATGATGGAGAAAACCCCCAGCCAAAGTCCAGCAAAACGATTCCAGCAGTTCTATGTGCCATATGGAAAAGGGAAGGGATTTTCGGCTTCTTCAAGGGCTTGCATGCTCAGATCTTGAAGACCGTCCTGAGCTCGGCATTGCTTCTGATGATCAAGGAAAAGATCTCCGCCGCTACTTGGGTTCTTCTACTTTCAATCAGAAGGTCTCTATTGCTCACAAGGGGCAGACTAAAAAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005777 GO:0005778 GO:0005779 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0007275 GO:0008150 GO:0008152 GO:0008509 GO:0008514 GO:0009056 GO:0009062 GO:0009791 GO:0009850 GO:0009987 GO:0010817 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0016042 GO:0016054 GO:0019395 GO:0019752 GO:0019866 GO:0022857 GO:0030258 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032787 GO:0034220 GO:0034440 GO:0034641 GO:0042221 GO:0042430 GO:0042445 GO:0042493 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046483 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0055114 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071705 GO:0072329 GO:0080024 GO:0090351 GO:0098588 GO:0098656 GO:0098805 GO:1901264 GO:1901360 GO:1901505 GO:1901564 GO:1901575 GO:1901679
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

184

Amino Acids

19.98

Weight (kDa)

10.01

Isoelectric Point (pI)

28.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 68 - 159 5.6e-14 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 125
AccII CGCG 1 cut(s) 107
AciI CCGC 4 cut(s) 105, 270, 477, 480
AclWI GGATC 1 cut(s) 286
AfiI CCNNNNNNNGG 3 cut(s) 276, 442, 486
AgsI TTSAA 3 cut(s) 403, 427, 504
AluBI AGCT 4 cut(s) 60, 142, 299, 441
AluI AGCT 4 cut(s) 60, 142, 299, 441
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 2 cut(s) 241, 519
AlwI GGATC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 57, 299
BanII GRGCYC 1 cut(s) 443
BbsI GAAGAC 2 cut(s) 47, 434
Bbv12I GWGCWC 1 cut(s) 443
BbvI GCAGC 2 cut(s) 69, 286
BccI CCATC 3 cut(s) 71, 281, 308
BclI TGATCA 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 241, 519
BfaI CTAG 1 cut(s) 83
BglII AGATCT 2 cut(s) 420, 471
BisI GCNGC 3 cut(s) 58, 300, 480
BlsI GCNGC 3 cut(s) 59, 301, 481
BmsI GCATC 1 cut(s) 58
BpiI GAAGAC 2 cut(s) 47, 434
BpmI CTGGAG 1 cut(s) 177
Bpu10I CCTNAGC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 442, 486
Bse3DI GCAATG 1 cut(s) 446
BseLI CCNNNNNNNGG 3 cut(s) 276, 442, 486
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 3 cut(s) 163, 428, 431
BseXI GCAGC 2 cut(s) 69, 286
BseYI CCCAGC 1 cut(s) 327
BsgI GTGCAG 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 443
BslI CCNNNNNNNGG 3 cut(s) 276, 442, 486
BsmAI GTCTC 2 cut(s) 241, 519
Bso31I GGTCTC 1 cut(s) 519
Bsp1286I GDGCHC 1 cut(s) 443
Bsp143I GATC 5 cut(s) 136, 291, 420, 459, 471
BspACI CCGC 4 cut(s) 105, 270, 477, 480
BspCNI CTCAG 3 cut(s) 162, 429, 430
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 288
BspPI GGATC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 519
BsrDI GCAATG 1 cut(s) 446
BssMI GATC 5 cut(s) 136, 291, 420, 459, 471
BssNAI GTATAC 1 cut(s) 126
Bst1107I GTATAC 1 cut(s) 126
Bst4CI ACNGT 4 cut(s) 130, 247, 256, 433
BstC8I GCNNGC 2 cut(s) 410, 414
BstDEI CTNAG 3 cut(s) 149, 417, 437
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 5 cut(s) 139, 294, 423, 462, 474
BstMAI GTCTC 2 cut(s) 241, 519
BstMBI GATC 5 cut(s) 136, 291, 420, 459, 471
BstMWI GCNNNNNNNGC 3 cut(s) 113, 206, 215
BstNSI RCATGY 1 cut(s) 416
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 69, 286
BstV2I GAAGAC 2 cut(s) 47, 434
BstX2I RGATCY 2 cut(s) 420, 471
BstYI RGATCY 2 cut(s) 420, 471
BstZ17I GTATAC 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 135, 252
Cac8I GCNNGC 2 cut(s) 410, 414
CciI TCATGA 1 cut(s) 288
CviAII CATG 2 cut(s) 289, 413
CviJI RGCY 9 cut(s) 33, 60, 142, 200, 299, 331, 396, 408, 441
CviKI_1 RGCY 9 cut(s) 33, 60, 142, 200, 299, 331, 396, 408, 441
DdeI CTNAG 3 cut(s) 149, 417, 437
DpnI GATC 5 cut(s) 138, 293, 422, 461, 473
DpnII GATC 5 cut(s) 136, 291, 420, 459, 471
EciI GGCGGA 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 443
Eco31I GGTCTC 1 cut(s) 519
Eco53kI GAGCTC 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 441
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 443
FaeI CATG 2 cut(s) 292, 416
FaiI YATR 6 cut(s) 126, 290, 364, 371, 373, 414
FatI CATG 2 cut(s) 288, 412
FauI CCCGC 1 cut(s) 277
FauNDI CATATG 1 cut(s) 371
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 58, 300, 480
FriOI GRGCYC 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 58, 300, 480
FspBI CTAG 1 cut(s) 83
GluI GCNGC 3 cut(s) 58, 300, 480
GsaI CCCAGC 1 cut(s) 331
GsuI CTGGAG 1 cut(s) 177
Hin1II CATG 2 cut(s) 292, 416
HinfI GANTC 5 cut(s) 43, 87, 153, 185, 349
Hpy166II GTNNAC 1 cut(s) 126
Hpy188I TCNGA 5 cut(s) 152, 261, 420, 456, 509
Hpy188III TCNNGA 5 cut(s) 157, 194, 289, 424, 436
Hpy8I GTNNAC 1 cut(s) 126
HpyAV CCTTC 3 cut(s) 220, 377, 504
HpyCH4III ACNGT 4 cut(s) 130, 247, 256, 433
HpyCH4V TGCA 5 cut(s) 4, 71, 116, 233, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 206, 215
HpyF3I CTNAG 3 cut(s) 149, 417, 437
Hsp92II CATG 2 cut(s) 292, 416
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 5 cut(s) 136, 291, 420, 459, 471
Lsp1109I GCAGC 2 cut(s) 69, 286
LweI GCATC 1 cut(s) 58
MaeI CTAG 1 cut(s) 83
MalI GATC 5 cut(s) 138, 293, 422, 461, 473
MboI GATC 5 cut(s) 136, 291, 420, 459, 471
MboII GAAGA 7 cut(s) 52, 178, 180, 320, 391, 439, 485
MflI RGATCY 2 cut(s) 420, 471
MhlI GDGCHC 1 cut(s) 443
MluCI AATT 1 cut(s) 117
MlyI GAGTC 2 cut(s) 37, 147
MnlI CCTC 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 60
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 113, 206, 215
NdeI CATATG 1 cut(s) 371
NdeII GATC 5 cut(s) 136, 291, 420, 459, 471
NlaIII CATG 2 cut(s) 292, 416
NmeAIII GCCGAG 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PagI TCATGA 1 cut(s) 288
PfeI GAWTC 3 cut(s) 87, 185, 349
PkrI GCNGC 3 cut(s) 59, 301, 481
PleI GAGTC 2 cut(s) 37, 147
PpsI GAGTC 2 cut(s) 37, 147
Psp124BI GAGCTC 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 327
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PsuI RGATCY 2 cut(s) 420, 471
PvuII CAGCTG 1 cut(s) 60
SacI GAGCTC 1 cut(s) 443
SatI GCNGC 3 cut(s) 58, 300, 480
Sau3AI GATC 5 cut(s) 136, 291, 420, 459, 471
SchI GAGTC 2 cut(s) 37, 147
SduI GDGCHC 1 cut(s) 443
SetI ASST 9 cut(s) 62, 144, 232, 266, 281, 288, 301, 443, 515
SfaNI GCATC 1 cut(s) 58
SphI GCATGC 1 cut(s) 416
Sse9I AATT 1 cut(s) 117
SsiI CCGC 4 cut(s) 105, 270, 477, 480
SspMI CTAG 1 cut(s) 83
SstI GAGCTC 1 cut(s) 443
TaaI ACNGT 4 cut(s) 130, 247, 256, 433
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 117
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 3 cut(s) 87, 185, 349
TscAI CASTG 2 cut(s) 135, 259
TseI GCWGC 2 cut(s) 57, 299
TspRI CASTG 2 cut(s) 135, 259
XceI RCATGY 1 cut(s) 416
XmiI GTMKAC 1 cut(s) 125
XspI CTAG 1 cut(s) 83
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.