Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

957280 .. 966734

Gene Structure 9455 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGGCTCGTCACAGAGACGAATATGACGACGAGATGGACCCCGATGAAGAGGAAGAATACGAGCCCGACCAAGCTATAGACGAGGATGACGAGGAGGAAGAGGAGGAGGAACGAGGAGGAGGACGACAGCGGTCCAGTCAGAAGCGGAGGAGATCGGATTTCATAGACGATGTAGCAGAGGAGGACGATGACGAGGAGGAAGAGGAGGGCTATGAGGATGACGAGCCTTATGGTGGTGGTGGTGCGAGTCGTAAGCGGAGGAATAAGAGGCCTTCTGGCTCGCAGTTTCTCGACATCGAGGCCGAGGTCGATACCGATGAAGAGGAAGAAGACGAGGAGGGCGAGGACGACTTTATTGCTGATAATGCACCTGATCTACCTGAAGATGAGGATGGTAGAAGGATGCGTCATCGTCCGTTGATGCCGCAAGAAGATGAGCAAGAAGATTTTGAAGAGATTGCAAAAAGTATTCAAGCGCGGTATGCGAAGTCAAGTCATACAGAGTATGATGAAGACACCACTGATGTGGATCAGCAAGCACTTTTACCTTCTGTTAGGGACCCAAAGTTGTGGATGGTGAAATGTGCGATTGGCCGTGAGCGGGAGGTAGCTGTTTGCTTAATGCAAAAGTATATTGATAAACCTGAACTGCAGATCAGGTCTGTTATTGCTCTCGACCACCTTAAAAACTATGTATATGTTGAAGCGGATAAAGAAGCTCATGTGAGGGAGGCTTGCAAAGGTCTGCGCAATATATTTGGCCAGAAAATAATGCTTGTTCCAATTAGAGAAATGACTGACGTTCTTTCTGTTGAAAGCAAGGCTATTGATCTCTCTAGGGATACATGGGTCAGAATGAAGACTGGGACATATAAAGGAGACCTTGCTCAGGTTGCGGATGTGGACAATGTGCGGCAGAGAGTTACAGTTAAATTAATTCCAAGGATTGATTTACAAGCTCTTGCAAATAAAATGGATGGCAGAGAAGTTGTGAGAACGAAAGCATTTGTTCCTCCTCCACGTTTACTAAATATTGAGGAAGCCAGGGAACTGCATATTCTTGGAGAGCGTAAACAAGATAGGATGACTGGTGATTATTATCAGACAATTAATGGCATGATGTTTAAAGATGGTTTCCTGTATAAAGCAGTATCAATCAAATCAATTAGTACTCAGAACATACATCCAACTTTTGACGAACTTGAAAAATTTCGGAAGCCCAGCGAGAATGGTGATGGAGATATTGCCAGTCTGTCAACTTTATTTTCAACCAGAAAGAAGGGACACTTTCTGAAGGGTGATGCAGTTATTGTTGTCAAGGGAGATCTAAAAAATTTGAAAGGATGGGTTGAGAAAGTTGAGGAGGAGACTGTTCATATCAGACCCGAAATGAAAGAACTCCCAAAAACACTTGCCATAAATGAAAAAGAGCTTTGCAAATACTTTGAGCCTGGGAATCATGTGAAAGTTGTATCTGGCACTCAGGAAGGTGCAACTGGTATGGTTGTTAAGGTTGAGCAGCATGTTCTCATTATCTTATCTGATGTAACGAAAGAACATATCCGTGTGTTTGCTGATGATGTTGTGGAGAGCTCTGAGGTGACATCTGGTATTACCAGAATTGGGGCATATGAGATTCATGATCTCGTGCTACTAGATAACAATAGCTTTGGTGTAATCATACGCGTAGAAAGTGAAGCATTTCAGGTTCTTAAGGGAGTTCCTGATAGACCCGAGGTTGCCCTTGTCAAGTTAAGAGAGGTCAAATGCAAGATTGAGAAAAGTTTTCCTGTGGAAGTTAAGTATAAACACAAAGTATCTGTGAAGGATGTTGTGAGGGTCATTGATGGCCCTTGCGAGAGGGGAAACAGGGTCCTGTGCAACACATATACAGGGGAGTCTTGTTCATTTTTGATCGACCATCACCTGGAGCATGCAGGCTTTATCTGTGTTAAGTCTCATAATTGTGCTCTTGTGGGAGGTTCACGTGCTAATGGCGATAGAAATGGTAACTCAAATTCAAGATATGACCACCTCAGAACTCCTCCTCGTGTTCCTCAGTCCCCGAATAGGTTTTCTAGAGGAGGCCCTCCATCTAACTATGGAGGAAGGAATAGAGGTGGACGAGGGCATGATGGCTTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGCTATCGTGGACGTGTTGTTGAGGTTAAGGGACCAAATGTACGGGTTGAGTTGGAGTCCCAAATGAAGGTTGTTACAGTTGATCGAAATTGTATTTCTGATAATGTGACTGTTACCACACCATATCGTGATACATCTCGCTACGGTATGGGAAGTGAGACTCCTATGCATCCTTCTCGAACTCCTCTCCACCCATATATGACTCCTATGAGGGATGCTGGAGCAACACCTATACATGATGGCATGAGGACTCCTATGCGTGACCGAGCATGGAATCCGTATGCACCTATGAGTCCAGCTAGGGATAACTGGGAGGAAGGAAATCCTGCTTCTTGGGGTGCTAGCCCACAGTATCAGCCAGGAAGTCCTCCTTCGCGAGCGTATGAAGCACCAACTCCCGGTTCAGGTTGGGCTAACACTCCTGGCGGAAATTACAGTGAAGCTGGCACACCGAGGGACAGTAGTTCTGGTTATGCAAATGCTCCTAGCCCTTACTTGCCATCGACTCCGGGTGGCCAGCCTATGACACCAAATTCGGCATCCTATCTTCCTGGAACTCCTGGAGGGCAGCCAATGACACCAGGCACTGGTGGTCTGGATATGTTGTCTCCTGTTATAGGTGGGGACAGTGAAGGACCGTGGTTCCTCCCAGACATATTGGTCAATGTACGCAATTCTGGAGAGGAAACTACTGGAGTTGTGAGAGAAGTGCTTCCGGATGGCTCATGTAGGGTAGCTATTGGGTCGGGTGGCAATGGGGAAACTATAGTGGCACTTCCTAATGAAATGGAGGCAGTGGTACCAAAGAAAAGTGATAAGATCAAGATCATGGGCGGGTCACTACGAGGAGCGACTGGTAAGCTGATTGGTGTTGATGGCACAGACGGAATTGTGAAGGTAGATGACACTCTTGATGTTAAGATATTAGACTTGGCTATTTTGTCGAAACTTGCTCAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1048

Amino Acids

116.25

Weight (kDa)

5.1

Isoelectric Point (pI)

44.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	96 - 183	9.4e-17	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	189 - 269	1.4e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	275 - 404	9.9e-69	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	432 - 481	1.2e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	540 - 591	1.5e-16	Transcription elongation factor SPT5, KOWx domain
KOW5_SPT5	PF23290	714 - 757	1.7e-17	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	942 - 991	1.9e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	993 - 1044	8.8e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2840
Acc16I	TGCGCA	1 cut(s)	749
Acc65I	GGTACC	1 cut(s)	2980
AccB1I	GGYRCC	2 cut(s)	2171, 2980
AccB7I	CCANNNNNTGG	2 cut(s)	1927, 2768
AccBSI	CCGCTC	1 cut(s)	601
AccII	CGCG	3 cut(s)	478, 1686, 2557
AccIII	TCCGGA	1 cut(s)	2896
AclWI	GGATC	1 cut(s)	537
AcoI	YGGCCR	3 cut(s)	592, 760, 2695
AcsI	RAATTY	4 cut(s)	1208, 1333, 2017, 2713
AcuI	CTGAAG	2 cut(s)	402, 1313
AcvI	CACGTG	1 cut(s)	1988
AfaI	GTAC	4 cut(s)	1171, 2223, 2850, 2982
AflII	CTTAAG	1 cut(s)	1712
AflIII	ACRYGT	2 cut(s)	1684, 2194
AgsI	TTSAA	8 cut(s)	452, 473, 704, 815, 1205, 1269, 1339, 2022
AjiI	CACGTC	1 cut(s)	2195
AjnI	CCWGG	9 cut(s)	1043, 1450, 1926, 2166, 2539, 2602, 2731, 2740, 2761
AjuI	GAANNNNNNNTTGG	2 cut(s)	2566, 2598
AleI	CACNNNNGTG	1 cut(s)	524
Alw21I	GWGCWC	2 cut(s)	1595, 1972
Alw26I	GTCTC	6 cut(s)	9, 873, 1361, 1962, 2333, 2793
AlwI	GGATC	1 cut(s)	537
AlwNI	CAGNNNCTG	1 cut(s)	2768
Ama87I	CYCGRG	1 cut(s)	1733
Aor13HI	TCCGGA	1 cut(s)	2896
AoxI	GGCC	7 cut(s)	269, 300, 592, 760, 1849, 2086, 2695
ApeKI	GCWGC	2 cut(s)	1519, 2749
ApoI	RAATTY	4 cut(s)	1208, 1333, 2017, 2713
ArsI	GACNNNNNNTTYG	2 cut(s)	2698, 2730
AseI	ATTAAT	2 cut(s)	935, 1110
Asp700I	GAANNNNTTC	3 cut(s)	1209, 1701, 2892
Asp718I	GGTACC	1 cut(s)	2980
AspLEI	GCGC	2 cut(s)	478, 750
AspS9I	GGNCC	8 cut(s)	37, 132, 559, 1850, 1873, 2087, 2213, 2816
AsuC2I	CCSGG	2 cut(s)	2580, 2691
AsuHPI	GGTGA	6 cut(s)	589, 1103, 1244, 1310, 1612, 1916
AsuNHI	GCTAGC	1 cut(s)	2522
AvaI	CYCGRG	1 cut(s)	1733
AvaII	GGWCC	6 cut(s)	37, 132, 559, 1873, 2213, 2816
BaeI	ACNNNNGTAYC	2 cut(s)	834, 867
BalI	TGGCCA	2 cut(s)	762, 2697
BanI	GGYRCC	2 cut(s)	2171, 2980
BanII	GRGCYC	2 cut(s)	66, 1595
BauI	CACGAG	2 cut(s)	1646, 2049
BbrPI	CACGTG	1 cut(s)	1988
BbsI	GAAGAC	3 cut(s)	336, 519, 866
Bbv12I	GWGCWC	2 cut(s)	1595, 1972
BbvI	GCAGC	2 cut(s)	1531, 2761
BceAI	ACGGC	1 cut(s)	579
BcgI	CGANNNNNNTGC	4 cut(s)	338, 372, 2339, 2373
BciT130I	CCWGG	9 cut(s)	1045, 1452, 1928, 2168, 2541, 2604, 2733, 2742, 2763
BciVI	GTATCC	1 cut(s)	835
BcnI	CCSGG	2 cut(s)	2580, 2691
BcoDI	GTCTC	6 cut(s)	9, 873, 1361, 1962, 2333, 2793
BfaI	CTAG	6 cut(s)	837, 1655, 2079, 2481, 2523, 2667
BfmI	CTRYAG	4 cut(s)	75, 650, 2151, 2946
BfrI	CTTAAG	1 cut(s)	1712
BfuI	GTATCC	1 cut(s)	835
BglII	AGATCT	1 cut(s)	1324
BisI	GCNGC	4 cut(s)	425, 914, 1520, 2750
BlsI	GCNGC	4 cut(s)	426, 915, 1521, 2751
BmcAI	AGTACT	1 cut(s)	1171
Bme18I	GGWCC	6 cut(s)	37, 132, 559, 1873, 2213, 2816
BmeT110I	CYCGRG	1 cut(s)	1733
BmgBI	CACGTC	1 cut(s)	2195
BmgT120I	GGNCC	8 cut(s)	37, 132, 559, 1850, 1873, 2087, 2213, 2816
BmiI	GGNNCC	8 cut(s)	39, 560, 561, 1874, 2173, 2214, 2825, 2982
BmrI	ACTGGG	2 cut(s)	873, 2500
BmsI	GCATC	6 cut(s)	393, 411, 1291, 2359, 2386, 2729
BmtI	GCTAGC	1 cut(s)	2526
BmuI	ACTGGG	2 cut(s)	873, 2500
BoxI	GACNNNNGTC	1 cut(s)	130
BpiI	GAAGAC	3 cut(s)	336, 519, 866
BpmI	CTGGAG	5 cut(s)	1949, 2421, 2763, 2880, 2895
Bpu10I	CCTNAGC	1 cut(s)	888
BpuMI	CCSGG	2 cut(s)	2580, 2691
BsaAI	YACGTR	1 cut(s)	1988
BsaBI	GATNNNNATC	3 cut(s)	528, 1098, 3005
BsaI	GGTCTC	1 cut(s)	873
BsaJI	CCNNGG	8 cut(s)	303, 941, 1044, 1451, 1734, 2167, 2633, 2819
BsaWI	WCCGGW	1 cut(s)	2896
Bse1I	ACTGG	9 cut(s)	135, 868, 1093, 1248, 1501, 2495, 2773, 2878, 3040
Bse3DI	GCAATG	1 cut(s)	2941
Bse8I	GATNNNNATC	3 cut(s)	528, 1098, 3005
BseAI	TCCGGA	1 cut(s)	2896
BseBI	CCWGG	9 cut(s)	1045, 1452, 1928, 2168, 2541, 2604, 2733, 2742, 2763
BseDI	CCNNGG	8 cut(s)	303, 941, 1044, 1451, 1734, 2167, 2633, 2819
BseJI	GATNNNNATC	3 cut(s)	528, 1098, 3005
BseMI	GCAATG	1 cut(s)	2941
BseMII	CTCAG	6 cut(s)	902, 1187, 1496, 1587, 2050, 2072
BseNI	ACTGG	9 cut(s)	135, 868, 1093, 1248, 1501, 2495, 2773, 2878, 3040
BseXI	GCAGC	2 cut(s)	1531, 2761
BseYI	CCCAGC	1 cut(s)	1220
Bsh1236I	CGCG	3 cut(s)	478, 1686, 2557
BshFI	GGCC	7 cut(s)	271, 302, 594, 762, 1851, 2088, 2697
BshNI	GGYRCC	2 cut(s)	2171, 2980
BsiHKAI	GWGCWC	2 cut(s)	1595, 1972
BsiHKCI	CYCGRG	1 cut(s)	1733
BsiSI	CCGG	3 cut(s)	2580, 2690, 2897
BslFI	GGGAC	9 cut(s)	572, 880, 1296, 2047, 2161, 2224, 2226, 2651, 2819
BsmAI	GTCTC	6 cut(s)	9, 873, 1361, 1962, 2333, 2793
BsmBI	CGTCTC	1 cut(s)	9
BsmFI	GGGAC	9 cut(s)	572, 880, 1296, 2047, 2161, 2224, 2226, 2651, 2819
BsnI	GGCC	7 cut(s)	271, 302, 594, 762, 1851, 2088, 2697
Bso31I	GGTCTC	1 cut(s)	873
BsoBI	CYCGRG	1 cut(s)	1733
Bsp1286I	GDGCHC	3 cut(s)	66, 1595, 1972
Bsp13I	TCCGGA	1 cut(s)	2896
Bsp68I	TCGCGA	1 cut(s)	2557
BspANI	GGCC	7 cut(s)	271, 302, 594, 762, 1851, 2088, 2697
BspCNI	CTCAG	6 cut(s)	901, 1186, 1495, 1588, 2049, 2071
BspEI	TCCGGA	1 cut(s)	2896
BspFNI	CGCG	3 cut(s)	478, 1686, 2557
BspHI	TCATGA	1 cut(s)	1639
BspLI	GGNNCC	8 cut(s)	39, 560, 561, 1874, 2173, 2214, 2825, 2982
BspMAI	CTGCAG	1 cut(s)	654
BspOI	GCTAGC	1 cut(s)	2526
BspPI	GGATC	1 cut(s)	537
BspT107I	GGYRCC	2 cut(s)	2171, 2980
BspTI	CTTAAG	1 cut(s)	1712
BspTNI	GGTCTC	1 cut(s)	873
BsrBI	CCGCTC	1 cut(s)	601
BsrDI	GCAATG	1 cut(s)	2941
BsrI	ACTGG	9 cut(s)	135, 868, 1093, 1248, 1501, 2495, 2773, 2878, 3040
BssECI	CCNNGG	8 cut(s)	303, 941, 1044, 1451, 1734, 2167, 2633, 2819
BssSI	CACGAG	2 cut(s)	1646, 2049
BssT1I	CCWWGG	1 cut(s)	941
Bst2BI	CACGAG	2 cut(s)	1646, 2049
Bst2UI	CCWGG	9 cut(s)	1045, 1452, 1928, 2168, 2541, 2604, 2733, 2742, 2763
Bst6I	CTCTTC	5 cut(s)	42, 93, 195, 315, 447
BstAFI	CTTAAG	1 cut(s)	1712
BstBAI	YACGTR	1 cut(s)	1988
BstC8I	GCNNGC	9 cut(s)	281, 537, 736, 1935, 1939, 2524, 2559, 2626, 2699
BstDEI	CTNAG	6 cut(s)	888, 1173, 1482, 1596, 2036, 2058
BstDSI	CCRYGG	1 cut(s)	2819
BstENI	CCTNNNNNAGG	2 cut(s)	887, 2796
BstFNI	CGCG	3 cut(s)	478, 1686, 2557
BstHHI	GCGC	2 cut(s)	478, 750
BstMAI	GTCTC	6 cut(s)	9, 873, 1361, 1962, 2333, 2793
BstMWI	GCNNNNNNNGC	4 cut(s)	365, 482, 893, 2567
BstNI	CCWGG	9 cut(s)	1045, 1452, 1928, 2168, 2541, 2604, 2733, 2742, 2763
BstNSI	RCATGY	2 cut(s)	1526, 1937
BstPAI	GACNNNNGTC	1 cut(s)	130
BstSFI	CTRYAG	4 cut(s)	75, 650, 2151, 2946
BstUI	CGCG	3 cut(s)	478, 1686, 2557
BstV1I	GCAGC	2 cut(s)	1531, 2761
BstV2I	GAAGAC	3 cut(s)	336, 519, 866
BstX2I	RGATCY	1 cut(s)	1324
BstXI	CCANNNNNNTGG	2 cut(s)	526, 570
BstYI	RGATCY	1 cut(s)	1324
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	7 cut(s)	271, 302, 594, 762, 1851, 2088, 2697
BtgI	CCRYGG	1 cut(s)	2819
BtrI	CACGTC	1 cut(s)	2195
BtsI	GCAGTG	1 cut(s)	2982
BtsIMutI	CAGTG	5 cut(s)	519, 2623, 2766, 2815, 2982
BtuMI	TCGCGA	1 cut(s)	2557
Cac8I	GCNNGC	9 cut(s)	281, 537, 736, 1935, 1939, 2524, 2559, 2626, 2699
CaiI	CAGNNNCTG	1 cut(s)	2768
CciI	TCATGA	1 cut(s)	1639
CfoI	GCGC	2 cut(s)	478, 750
Cfr13I	GGNCC	8 cut(s)	37, 132, 559, 1850, 1873, 2087, 2213, 2816
CseI	GACGC	1 cut(s)	395
Csp6I	GTAC	4 cut(s)	1170, 2222, 2849, 2981
CviQI	GTAC	4 cut(s)	1170, 2222, 2849, 2981
DdeI	CTNAG	6 cut(s)	888, 1173, 1482, 1596, 2036, 2058
DraI	TTTAAA	1 cut(s)	1126
DrdI	GACNNNNNNGTC	1 cut(s)	2840
DseDI	GACNNNNNNGTC	1 cut(s)	2840
EaeI	YGGCCR	3 cut(s)	592, 760, 2695
Eam1104I	CTCTTC	5 cut(s)	42, 93, 195, 315, 447
EarI	CTCTTC	5 cut(s)	42, 93, 195, 315, 447
EciI	GGCGGA	1 cut(s)	2622
Ecl136II	GAGCTC	1 cut(s)	1593
Eco130I	CCWWGG	1 cut(s)	941
Eco147I	AGGCCT	1 cut(s)	271
Eco24I	GRGCYC	2 cut(s)	66, 1595
Eco31I	GGTCTC	1 cut(s)	873
Eco47I	GGWCC	6 cut(s)	37, 132, 559, 1873, 2213, 2816
Eco53kI	GAGCTC	1 cut(s)	1593
Eco57I	CTGAAG	2 cut(s)	402, 1313
Eco72I	CACGTG	1 cut(s)	1988
Eco88I	CYCGRG	1 cut(s)	1733
EcoICRI	GAGCTC	1 cut(s)	1593
EcoNI	CCTNNNNNAGG	2 cut(s)	887, 2796
EcoO109I	RGGNCCY	3 cut(s)	559, 1873, 2087
EcoRII	CCWGG	9 cut(s)	1043, 1450, 1926, 2166, 2539, 2602, 2731, 2740, 2761
EcoT14I	CCWWGG	1 cut(s)	941
EcoT22I	ATGCAT	1 cut(s)	2352
EcoT38I	GRGCYC	2 cut(s)	66, 1595
ErhI	CCWWGG	1 cut(s)	941
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	6 cut(s)	867, 899, 1271, 1303, 2536, 2568
FaqI	GGGAC	9 cut(s)	572, 880, 1296, 2047, 2161, 2224, 2226, 2651, 2819
FauI	CCCGC	2 cut(s)	594, 3008
FauNDI	CATATG	1 cut(s)	1630
Fnu4HI	GCNGC	4 cut(s)	425, 914, 1520, 2750
FriOI	GRGCYC	2 cut(s)	66, 1595
Fsp4HI	GCNGC	4 cut(s)	425, 914, 1520, 2750
FspBI	CTAG	6 cut(s)	837, 1655, 2079, 2481, 2523, 2667
FspI	TGCGCA	1 cut(s)	749
GlaI	GCGC	2 cut(s)	477, 749
GluI	GCNGC	4 cut(s)	425, 914, 1520, 2750
GsaI	CCCAGC	1 cut(s)	1224
GsuI	CTGGAG	5 cut(s)	1949, 2421, 2763, 2880, 2895
HaeIII	GGCC	7 cut(s)	271, 302, 594, 762, 1851, 2088, 2697
HapII	CCGG	3 cut(s)	2580, 2690, 2897
HgaI	GACGC	1 cut(s)	395
HhaI	GCGC	2 cut(s)	478, 750
Hin6I	GCGC	2 cut(s)	476, 748
HinP1I	GCGC	2 cut(s)	476, 748
HincII	GTYRAC	1 cut(s)	1257
HindII	GTYRAC	1 cut(s)	1257
HpaII	CCGG	3 cut(s)	2580, 2690, 2897
HphI	GGTGA	6 cut(s)	589, 1103, 1244, 1310, 1612, 1916
Hpy166II	GTNNAC	7 cut(s)	904, 1025, 1073, 1257, 1985, 2123, 2192
Hpy8I	GTNNAC	7 cut(s)	904, 1025, 1073, 1257, 1985, 2123, 2192
Hpy99I	CGWCG	1 cut(s)	32
HpyCH4IV	ACGT	4 cut(s)	801, 1021, 1987, 2194
HpyF10VI	GCNNNNNNNGC	4 cut(s)	365, 482, 893, 2567
HpyF3I	CTNAG	6 cut(s)	888, 1173, 1482, 1596, 2036, 2058
HpySE526I	ACGT	4 cut(s)	801, 1021, 1987, 2194
HspAI	GCGC	2 cut(s)	476, 748
KflI	GGGWCCC	1 cut(s)	559
Kpn2I	TCCGGA	1 cut(s)	2896
KpnI	GGTACC	1 cut(s)	2984
LmnI	GCTCC	4 cut(s)	1930, 2402, 2668, 3029
Lsp1109I	GCAGC	2 cut(s)	1531, 2761
LweI	GCATC	6 cut(s)	393, 411, 1291, 2359, 2386, 2729
MaeI	CTAG	6 cut(s)	837, 1655, 2079, 2481, 2523, 2667
MaeII	ACGT	4 cut(s)	801, 1021, 1987, 2194
MbiI	CCGCTC	1 cut(s)	601
MflI	RGATCY	1 cut(s)	1324
MhlI	GDGCHC	3 cut(s)	66, 1595, 1972
MlsI	TGGCCA	2 cut(s)	762, 2697
MluI	ACGCGT	1 cut(s)	1684
MluNI	TGGCCA	2 cut(s)	762, 2697
MlyI	GAGTC	8 cut(s)	256, 1907, 2246, 2335, 2377, 2425, 2482, 2680
MmeI	TCCRAC	2 cut(s)	1211, 2214
Mox20I	TGGCCA	2 cut(s)	762, 2697
Mph1103I	ATGCAT	1 cut(s)	2352
MroI	TCCGGA	1 cut(s)	2896
MroXI	GAANNNNTTC	3 cut(s)	1209, 1701, 2892
MscI	TGGCCA	2 cut(s)	762, 2697
MslI	CAYNNNNRTG	3 cut(s)	524, 1563, 1965
Msp20I	TGGCCA	2 cut(s)	762, 2697
MspA1I	CMGCKG	1 cut(s)	130
MspCI	CTTAAG	1 cut(s)	1712
MspI	CCGG	3 cut(s)	2580, 2690, 2897
MvaI	CCWGG	9 cut(s)	1045, 1452, 1928, 2168, 2541, 2604, 2733, 2742, 2763
MvnI	CGCG	3 cut(s)	478, 1686, 2557
MwoI	GCNNNNNNNGC	4 cut(s)	365, 482, 893, 2567
NciI	CCSGG	2 cut(s)	2580, 2691
NdeI	CATATG	1 cut(s)	1630
NheI	GCTAGC	1 cut(s)	2522
NlaIV	GGNNCC	8 cut(s)	39, 560, 561, 1874, 2173, 2214, 2825, 2982
NmeAIII	GCCGAG	1 cut(s)	328
NmuCI	GTSAC	5 cut(s)	8, 1600, 2287, 2441, 3018
NruI	TCGCGA	1 cut(s)	2557
NsbI	TGCGCA	1 cut(s)	749
NsiI	ATGCAT	1 cut(s)	2352
NspI	RCATGY	2 cut(s)	1526, 1937
OliI	CACNNNNGTG	1 cut(s)	524
PaeI	GCATGC	1 cut(s)	1937
PagI	TCATGA	1 cut(s)	1639
PceI	AGGCCT	1 cut(s)	271
PcsI	WCGNNNNNNNCGW	3 cut(s)	24, 87, 339
PdmI	GAANNNNTTC	3 cut(s)	1209, 1701, 2892
PfeI	GAWTC	4 cut(s)	1456, 1636, 2161, 2455
PflMI	CCANNNNNTGG	2 cut(s)	1927, 2768
PfoI	TCCNGGA	2 cut(s)	2731, 2740
PkrI	GCNGC	4 cut(s)	426, 915, 1521, 2751
PleI	GAGTC	8 cut(s)	255, 1906, 2245, 2335, 2377, 2425, 2481, 2680
PmaCI	CACGTG	1 cut(s)	1988
PmlI	CACGTG	1 cut(s)	1988
PpsI	GAGTC	8 cut(s)	255, 1906, 2245, 2335, 2377, 2425, 2481, 2680
Ppu21I	YACGTR	1 cut(s)	1988
PpuMI	RGGWCCY	2 cut(s)	559, 1873
PshAI	GACNNNNGTC	1 cut(s)	130
PshBI	ATTAAT	2 cut(s)	935, 1110
Psp124BI	GAGCTC	1 cut(s)	1595
Psp5II	RGGWCCY	2 cut(s)	559, 1873
Psp6I	CCWGG	9 cut(s)	1043, 1450, 1926, 2166, 2539, 2602, 2731, 2740, 2761
PspCI	CACGTG	1 cut(s)	1988
PspFI	CCCAGC	1 cut(s)	1220
PspGI	CCWGG	9 cut(s)	1043, 1450, 1926, 2166, 2539, 2602, 2731, 2740, 2761
PspN4I	GGNNCC	8 cut(s)	39, 560, 561, 1874, 2173, 2214, 2825, 2982
PspPI	GGNCC	8 cut(s)	37, 132, 559, 1850, 1873, 2087, 2213, 2816
PspPPI	RGGWCCY	2 cut(s)	559, 1873
PstI	CTGCAG	1 cut(s)	654
PstNI	CAGNNNCTG	1 cut(s)	2768
PsuI	RGATCY	1 cut(s)	1324
RruI	TCGCGA	1 cut(s)	2557
RsaI	GTAC	4 cut(s)	1171, 2223, 2850, 2982
RsaNI	GTAC	4 cut(s)	1170, 2222, 2849, 2981
RseI	CAYNNNNRTG	3 cut(s)	524, 1563, 1965
SacI	GAGCTC	1 cut(s)	1595
SatI	GCNGC	4 cut(s)	425, 914, 1520, 2750
Sau96I	GGNCC	8 cut(s)	37, 132, 559, 1850, 1873, 2087, 2213, 2816
ScaI	AGTACT	1 cut(s)	1171
SchI	GAGTC	8 cut(s)	256, 1907, 2246, 2335, 2377, 2425, 2482, 2680
SduI	GDGCHC	3 cut(s)	66, 1595, 1972
SfaNI	GCATC	6 cut(s)	393, 411, 1291, 2359, 2386, 2729
SfcI	CTRYAG	4 cut(s)	75, 650, 2151, 2946
SinI	GGWCC	6 cut(s)	37, 132, 559, 1873, 2213, 2816
SmiMI	CAYNNNNRTG	3 cut(s)	524, 1563, 1965
SmlI	CTYRAG	1 cut(s)	1712
SmoI	CTYRAG	1 cut(s)	1712
SphI	GCATGC	1 cut(s)	1937
SseBI	AGGCCT	1 cut(s)	271
SspI	AATATT	1 cut(s)	1033
SspMI	CTAG	6 cut(s)	837, 1655, 2079, 2481, 2523, 2667
SstI	GAGCTC	1 cut(s)	1595
StuI	AGGCCT	1 cut(s)	271
StyI	CCWWGG	1 cut(s)	941
TaiI	ACGT	4 cut(s)	804, 1024, 1990, 2197
TaqI	TCGA	9 cut(s)	291, 297, 309, 675, 1917, 2266, 2359, 2684, 3125
TaqII	GACCGA	1 cut(s)	2460
TatI	WGTACW	1 cut(s)	1169
TauI	GCSGC	2 cut(s)	427, 916
TfiI	GAWTC	4 cut(s)	1456, 1636, 2161, 2455
TscAI	CASTG	5 cut(s)	526, 2623, 2773, 2815, 2982
TseFI	GTSAC	5 cut(s)	8, 1600, 2287, 2441, 3018
TseI	GCWGC	2 cut(s)	1519, 2749
Tsp45I	GTSAC	5 cut(s)	8, 1600, 2287, 2441, 3018
TspGWI	ACGGA	4 cut(s)	405, 1553, 2448, 3081
TspRI	CASTG	5 cut(s)	526, 2623, 2773, 2815, 2982
Van91I	CCANNNNNTGG	2 cut(s)	1927, 2768
Vha464I	CTTAAG	1 cut(s)	1712
VpaK11BI	GGWCC	6 cut(s)	37, 132, 559, 1873, 2213, 2816
VspI	ATTAAT	2 cut(s)	935, 1110
XagI	CCTNNNNNAGG	2 cut(s)	887, 2796
XapI	RAATTY	4 cut(s)	1208, 1333, 2017, 2713
XbaI	TCTAGA	1 cut(s)	2078
XceI	RCATGY	2 cut(s)	1526, 1937
XmnI	GAANNNNTTC	3 cut(s)	1209, 1701, 2892
XspI	CTAG	6 cut(s)	837, 1655, 2079, 2481, 2523, 2667
ZrmI	AGTACT	1 cut(s)	1171
Zsp2I	ATGCAT	1 cut(s)	2352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.