pycom06g05140

Aminoacyl-tRNA editing domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002840
Physical Location & Seq
Reverse (-)
7046106 .. 7050166
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGGAAGACTCACTAGCAAAGCTGGAGCGAATCCAAACCCAAATCCTCGAACGCATATCAAAGCTTGAGAGCTCCACCTCCGCCTCTCTTCCCCAATCCACCTCCACCCCCATCCCTTCCTCAACTCAAAACGCCGCCGTCGGCGCCACCGAAGCCCGCCTCTCCGCCATTCTCCTCGCCAATGGCGTAAAAGACTTCGCCTTCAAGAGGGTTGCTTCGGATTACTACGATTGGTCCCTCGATGCCCGGCGCGACGCTCTCCGCGCTGCCTCAATCCACCATCTCTGCAAAAGCATTGTCTTGGTTAACACTCAGGCCCAATCCAGTGTCGTTGATTGTAGTGACCGCAACAATTCAAAGTACTATGTTGTTGTTGTTCAGTACACTGCTCGGTTTAATGCTGAAGCTGTTAAGAACTTCTTGTATTCGCTCAATGACGGCAAGATAGCGAAAAAGAAATTCAACATGAGGCTTGCGCCTGAGGATACTTCAATACAATTGACTGGGTTTGAGCACAATGCAGTTACATGTGTTGGCATGAAAACAGACATTCCAGTGATTATGGATGAAGCAATTGAGAAACTTAGTCCTGATTTCTTCTGGCTGGGCGGCGGCGAGGTTGATCTGAAGCTGGGCATCAGAACCTCAGAATTTATAAGCTATGCTAAACCTTTTATCGTGAGCTGCAGTGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_edit

Protein Analysis

233

Amino Acids

25.52

Weight (kDa)

6.83

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 92 - 218 3.9e-08 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 2 cut(s) 252, 264
AciI CCGC 8 cut(s) 81, 135, 157, 165, 262, 346, 609, 612
AcsI RAATTY 2 cut(s) 458, 650
AcuI CTGAAG 2 cut(s) 423, 647
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 2 cut(s) 362, 383
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 527
AgsI TTSAA 4 cut(s) 205, 357, 463, 492
AluBI AGCT 7 cut(s) 22, 64, 72, 407, 631, 660, 684
AluI AGCT 7 cut(s) 22, 64, 72, 407, 631, 660, 684
Alw21I GWGCWC 2 cut(s) 74, 516
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 266, 684
ApoI RAATTY 2 cut(s) 458, 650
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspLEI GCGC 4 cut(s) 146, 252, 266, 478
AspS9I GGNCC 2 cut(s) 234, 316
AsuC2I CCSGG 1 cut(s) 247
AvaII GGWCC 1 cut(s) 234
AxyI CCTNAGG 1 cut(s) 480
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 74, 516
BbvI GCAGC 2 cut(s) 253, 671
BccI CCATC 2 cut(s) 119, 288
BceAI ACGGC 2 cut(s) 122, 454
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 1 cut(s) 247
BfaI CTAG 1 cut(s) 14
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 1 cut(s) 147
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 5 cut(s) 135, 267, 610, 613, 685
BlsI GCNGC 5 cut(s) 136, 268, 611, 614, 686
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 234, 316
BmiI GGNNCC 2 cut(s) 145, 236
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 232, 645
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 3 cut(s) 324, 508, 554
Bse21I CCTNAGG 1 cut(s) 480
BseGI GGATG 2 cut(s) 111, 571
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 326, 471, 660
BseNI ACTGG 3 cut(s) 324, 508, 554
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 253, 671
BseYI CCCAGC 2 cut(s) 604, 631
Bsh1236I CGCG 2 cut(s) 252, 264
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 2 cut(s) 74, 516
BsiSI CCGG 1 cut(s) 247
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 74, 516
Bsp143I GATC 1 cut(s) 622
BspACI CCGC 8 cut(s) 81, 135, 157, 165, 262, 346, 609, 612
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 3 cut(s) 325, 472, 659
BspFNI CGCG 2 cut(s) 252, 264
BspLI GGNNCC 2 cut(s) 145, 236
BspMAI CTGCAG 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 143
BsrI ACTGG 3 cut(s) 324, 508, 554
BssMI GATC 1 cut(s) 622
BssNI GRCGYC 1 cut(s) 144
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 157, 474
BstDEI CTNAG 4 cut(s) 312, 480, 584, 646
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 111, 571
BstFNI CGCG 2 cut(s) 252, 264
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 4 cut(s) 146, 252, 266, 478
BstKTI GATC 1 cut(s) 625
BstMBI GATC 1 cut(s) 622
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 263
BstNSI RCATGY 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 685
BstUI CGCG 2 cut(s) 252, 264
BstV1I GCAGC 2 cut(s) 253, 671
BstV2I GAAGAC 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 480
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 111, 571
BtsI GCAGTG 2 cut(s) 384, 694
BtsIMutI CAGTG 4 cut(s) 331, 384, 561, 694
Cac8I GCNNGC 2 cut(s) 157, 474
CfoI GCGC 4 cut(s) 146, 252, 266, 478
Cfr13I GGNCC 2 cut(s) 234, 316
CseI GACGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 361, 382
CviAII CATG 3 cut(s) 466, 528, 538
CviQI GTAC 2 cut(s) 361, 382
DdeI CTNAG 4 cut(s) 312, 480, 584, 646
DinI GGCGCC 1 cut(s) 145
DpnI GATC 1 cut(s) 624
DpnII GATC 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 2 cut(s) 70, 154
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 423, 647
Eco81I CCTNAGG 1 cut(s) 480
EcoICRI GAGCTC 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 74
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
FaeI CATG 3 cut(s) 469, 531, 541
FaiI YATR 8 cut(s) 56, 366, 467, 529, 539, 563, 656, 663
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 465, 527, 537
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 5 cut(s) 135, 267, 610, 613, 685
FokI GGATG 2 cut(s) 98, 578
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 5 cut(s) 135, 267, 610, 613, 685
FspBI CTAG 1 cut(s) 14
GlaI GCGC 4 cut(s) 145, 251, 265, 477
GluI GCNGC 5 cut(s) 135, 267, 610, 613, 685
GsaI CCCAGC 2 cut(s) 608, 635
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 1 cut(s) 317
HapII CCGG 1 cut(s) 247
HgaI GACGC 1 cut(s) 263
HhaI GCGC 4 cut(s) 146, 252, 266, 478
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 469, 531, 541
Hin6I GCGC 4 cut(s) 144, 250, 264, 476
HinP1I GCGC 4 cut(s) 144, 250, 264, 476
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 2 cut(s) 8, 30
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 307, 384
Hpy188I TCNGA 4 cut(s) 220, 627, 641, 649
Hpy188III TCNNGA 4 cut(s) 205, 590, 679, 696
Hpy8I GTNNAC 2 cut(s) 307, 384
Hpy99I CGWCG 2 cut(s) 143, 257
HpyAV CCTTC 2 cut(s) 126, 211
HpyCH4V TGCA 3 cut(s) 288, 521, 687
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 263
HpyF3I CTNAG 4 cut(s) 312, 480, 584, 646
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 469, 531, 541
HspAI GCGC 4 cut(s) 144, 250, 264, 476
KasI GGCGCC 1 cut(s) 143
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 1 cut(s) 622
LmnI GCTCC 2 cut(s) 25, 77
Lsp1109I GCAGC 2 cut(s) 253, 671
LweI GCATC 2 cut(s) 232, 645
MaeI CTAG 1 cut(s) 14
MaeIII GTNAC 2 cut(s) 341, 523
MalI GATC 1 cut(s) 624
MboI GATC 1 cut(s) 622
MboII GAAGA 3 cut(s) 17, 80, 589
MfeI CAATTG 2 cut(s) 497, 573
MhlI GDGCHC 2 cut(s) 74, 516
MluCI AATT 5 cut(s) 352, 458, 497, 573, 650
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 2
MseI TTAA 3 cut(s) 306, 396, 411
MslI CAYNNNNRTG 1 cut(s) 554
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 2 cut(s) 497, 573
MvnI CGCG 2 cut(s) 252, 264
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 263
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 1 cut(s) 247
NdeII GATC 1 cut(s) 622
NlaIII CATG 3 cut(s) 469, 531, 541
NlaIV GGNNCC 2 cut(s) 145, 236
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 531
PciI ACATGT 1 cut(s) 527
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 183
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 5 cut(s) 136, 268, 611, 614, 686
PleI GAGTC 1 cut(s) 2
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 2
PscI ACATGT 1 cut(s) 527
PsiI TTATAA 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 74
PspFI CCCAGC 2 cut(s) 604, 631
PspN4I GGNNCC 2 cut(s) 145, 236
PspPI GGNCC 2 cut(s) 234, 316
PstI CTGCAG 1 cut(s) 689
RsaI GTAC 2 cut(s) 362, 383
RsaNI GTAC 2 cut(s) 361, 382
RseI CAYNNNNRTG 1 cut(s) 554
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 306, 396, 411
SatI GCNGC 5 cut(s) 135, 267, 610, 613, 685
Sau3AI GATC 1 cut(s) 622
Sau96I GGNCC 2 cut(s) 234, 316
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 74, 516
SfaNI GCATC 2 cut(s) 232, 645
SfcI CTRYAG 1 cut(s) 685
SfoI GGCGCC 1 cut(s) 145
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 5 cut(s) 352, 458, 497, 573, 650
SsiI CCGC 8 cut(s) 81, 135, 157, 165, 262, 346, 609, 612
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 245
TaqI TCGA 2 cut(s) 48, 240
TasI AATT 5 cut(s) 352, 458, 497, 573, 650
TatI WGTACW 2 cut(s) 360, 381
TauI GCSGC 3 cut(s) 137, 612, 615
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 3 cut(s) 306, 396, 411
Tru9I TTAA 3 cut(s) 306, 396, 411
TscAI CASTG 4 cut(s) 331, 391, 561, 694
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 266, 684
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 2 cut(s) 554, 582
TspRI CASTG 4 cut(s) 331, 391, 561, 694
VpaK11BI GGWCC 1 cut(s) 234
XagI CCTNNNNNAGG 1 cut(s) 205
XapI RAATTY 2 cut(s) 458, 650
XceI RCATGY 1 cut(s) 531
XspI CTAG 1 cut(s) 14
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.