pycom06g07440

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002094
Physical Location & Seq
Reverse (-)
12097833 .. 12100254
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGGTGGCCAAGAGCTCGCCGGACTGGCTCCCGCCCGGCTGGTCCGTCCAATCCAGAGACCAAAAGAGAGGTCGCAAAATTGAGTTTTATATCCATTTGGAAACTGGGAAAAAGTTCTTCTCTAAGGATGATGCTGTGCGCTTTATCAAAATGGAAAACACGCGCGGTAAAAAGCCTCAGCCAACTTCTGTGCAGATCCCAAACCACTCAGAAGAAGTTCCTTCTCAACTTGAAGTGGACCCAACCGAAAATCCTGAATGGTTGCCTAATGGTTGGAAAGTGGAGCTGAGGATACGACAGAGTGGTGTGCAAGTTGGAAGAGAATACAAGTGTTACATTGATCCCTCCAGAGAACGTACATTCTATTCCAAGCCAGAGGTATTTCGGTATCTCAAAACTGTAAAACGGAAGAGCTCGAAGTCTGGAAATCTCAAATCTATAAGCAGCAAGAGATGCATAACCGAAGGGAAGGATGTCACTACCATGCAGCTGACAAAAAATGTTTGTTTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

172

Amino Acids

19.9

Weight (kDa)

9.78

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 86 - 135 2e-07 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 163, 165
AciI CCGC 2 cut(s) 32, 165
AclWI GGATC 2 cut(s) 190, 335
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 1 cut(s) 358
AgsI TTSAA 1 cut(s) 233
AluBI AGCT 4 cut(s) 15, 286, 414, 490
AluI AGCT 4 cut(s) 15, 286, 414, 490
Alw21I GWGCWC 2 cut(s) 17, 416
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 2 cut(s) 190, 335
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 444, 487
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
Asp700I GAANNNNTTC 2 cut(s) 113, 216
AspLEI GCGC 2 cut(s) 141, 165
AspS9I GGNCC 2 cut(s) 42, 238
AsuC2I CCSGG 1 cut(s) 36
AvaII GGWCC 2 cut(s) 42, 238
BalI TGGCCA 1 cut(s) 8
BanII GRGCYC 2 cut(s) 17, 416
Bbv12I GWGCWC 2 cut(s) 17, 416
BbvCI CCTCAGC 2 cut(s) 177, 287
BbvI GCAGC 2 cut(s) 456, 499
BciVI GTATCC 1 cut(s) 285
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 51
BfuI GTATCC 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 2 cut(s) 445, 488
BlsI GCNGC 2 cut(s) 446, 489
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 2 cut(s) 42, 238
BmgT120I GGNCC 2 cut(s) 42, 238
BmiI GGNNCC 2 cut(s) 29, 240
BmrFI CCNGG 1 cut(s) 36
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 121, 443
BmuI ACTGGG 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 331
Bpu10I CCTNAGC 2 cut(s) 177, 287
BpuMI CCSGG 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 51
Bse1I ACTGG 2 cut(s) 29, 109
BseGI GGATG 2 cut(s) 133, 478
BseMII CTCAG 3 cut(s) 191, 222, 278
BseNI ACTGG 2 cut(s) 29, 109
BseXI GCAGC 2 cut(s) 456, 499
BsgI GTGCAG 1 cut(s) 212
Bsh1236I CGCG 2 cut(s) 163, 165
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 17, 416
BsiSI CCGG 2 cut(s) 20, 36
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 17, 416
Bsp143I GATC 2 cut(s) 195, 340
BspACI CCGC 2 cut(s) 32, 165
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 3 cut(s) 190, 221, 279
BspFNI CGCG 2 cut(s) 163, 165
BspLI GGNNCC 2 cut(s) 29, 240
BspPI GGATC 2 cut(s) 190, 335
BspQI GCTCTTC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 51
BsrI ACTGG 2 cut(s) 29, 109
BssMI GATC 2 cut(s) 195, 340
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 2 cut(s) 313, 404
BstAPI GCANNNNNTGC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 4 cut(s) 123, 177, 208, 287
BstF5I GGATG 2 cut(s) 133, 478
BstFNI CGCG 2 cut(s) 163, 165
BstHHI GCGC 2 cut(s) 141, 165
BstKTI GATC 2 cut(s) 198, 343
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 2 cut(s) 195, 340
BstMWI GCNNNNNNNGC 2 cut(s) 25, 453
BstSCI CCNGG 1 cut(s) 34
BstUI CGCG 2 cut(s) 163, 165
BstV1I GCAGC 2 cut(s) 456, 499
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 8
BtsCI GGATG 2 cut(s) 133, 478
Cac8I GCNNGC 1 cut(s) 17
CfoI GCGC 2 cut(s) 141, 165
Cfr13I GGNCC 2 cut(s) 42, 238
Csp6I GTAC 1 cut(s) 357
CviAII CATG 1 cut(s) 484
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 4 cut(s) 123, 177, 208, 287
DpnI GATC 2 cut(s) 197, 342
DpnII GATC 2 cut(s) 195, 340
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 313, 404
EarI CTCTTC 2 cut(s) 313, 404
Ecl136II GAGCTC 2 cut(s) 15, 414
Eco24I GRGCYC 2 cut(s) 17, 416
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 42, 238
Eco53kI GAGCTC 2 cut(s) 15, 414
EcoICRI GAGCTC 2 cut(s) 15, 414
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 2 cut(s) 17, 416
FaeI CATG 1 cut(s) 487
FaiI YATR 4 cut(s) 90, 440, 458, 485
FatI CATG 1 cut(s) 483
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 445, 488
FokI GGATG 2 cut(s) 140, 485
FriOI GRGCYC 2 cut(s) 17, 416
Fsp4HI GCNGC 2 cut(s) 445, 488
GlaI GCGC 2 cut(s) 140, 164
GluI GCNGC 2 cut(s) 445, 488
GsuI CTGGAG 1 cut(s) 331
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 20, 36
HhaI GCGC 2 cut(s) 141, 165
Hin1II CATG 1 cut(s) 487
Hin6I GCGC 2 cut(s) 139, 163
HinP1I GCGC 2 cut(s) 139, 163
HpaII CCGG 2 cut(s) 20, 36
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 4 cut(s) 54, 254, 348, 423
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 3 cut(s) 231, 458, 463
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 4 cut(s) 193, 310, 456, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 453
HpyF3I CTNAG 4 cut(s) 123, 177, 208, 287
HpySE526I ACGT 1 cut(s) 355
Hsp92II CATG 1 cut(s) 487
HspAI GCGC 2 cut(s) 139, 163
Kzo9I GATC 2 cut(s) 195, 340
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 2 cut(s) 33, 283
Lsp1109I GCAGC 2 cut(s) 456, 499
LweI GCATC 2 cut(s) 121, 443
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 2 cut(s) 332, 475
MalI GATC 2 cut(s) 197, 342
MboI GATC 2 cut(s) 195, 340
MboII GAAGA 4 cut(s) 109, 224, 330, 421
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 2 cut(s) 17, 416
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 1 cut(s) 78
MluNI TGGCCA 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 254, 295
MnlI CCTC 5 cut(s) 62, 186, 282, 355, 370
Mox20I TGGCCA 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 113, 216
MscI TGGCCA 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 482
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 2 cut(s) 20, 36
MspR9I CCNGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 163, 165
MwoI GCNNNNNNNGC 2 cut(s) 25, 453
NciI CCSGG 1 cut(s) 36
NdeII GATC 2 cut(s) 195, 340
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 2 cut(s) 29, 240
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 1 cut(s) 458
PciSI GCTCTTC 1 cut(s) 404
PdmI GAANNNNTTC 2 cut(s) 113, 216
PkrI GCNGC 2 cut(s) 446, 489
Psp124BI GAGCTC 2 cut(s) 17, 416
PspN4I GGNNCC 2 cut(s) 29, 240
PspPI GGNCC 2 cut(s) 42, 238
PsuI RGATCY 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 490
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 482
SacI GAGCTC 2 cut(s) 17, 416
SapI GCTCTTC 1 cut(s) 404
SatI GCNGC 2 cut(s) 445, 488
Sau3AI GATC 2 cut(s) 195, 340
Sau96I GGNCC 2 cut(s) 42, 238
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 2 cut(s) 17, 416
SetI ASST 7 cut(s) 17, 73, 288, 358, 381, 416, 492
SfaNI GCATC 2 cut(s) 121, 443
SinI GGWCC 2 cut(s) 42, 238
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 1 cut(s) 78
SsiI CCGC 2 cut(s) 32, 165
SstI GAGCTC 2 cut(s) 17, 416
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 358
TaqI TCGA 1 cut(s) 416
TasI AATT 1 cut(s) 78
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 444, 487
Tsp45I GTSAC 1 cut(s) 475
TspGWI ACGGA 2 cut(s) 34, 421
VpaK11BI GGWCC 2 cut(s) 42, 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XmnI GAANNNNTTC 2 cut(s) 113, 216
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.