pycom07g22490

phosphotransferase activity, carboxyl group as acceptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002381
Physical Location & Seq
Forward (+)
23821744 .. 23824490
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGGGATTCCACCCAGAGACACAGACGATGAACAACAAGGGCAAAGCAGTCGCCGTCTCAAGCCCCAACATCAACGACATCGACGACGACAACGTTAACATCAACGTAGACGACTTGGATTTGGACGACGACAACAACAACAGAAGTAGTGGCGTCTCTTCGACTTCCATCAGATTCTCGTCCCGCAACACTTCCTCCAAATACGACTTTGTCAAGGTGAAGGTCTGGCTGGGTGATAATGCAGATCACTACTATGTTCTTTCCAGATTTTTGCTCAGTCGAATGTTGACTGTCACTAAGATCCCGAATTATGTAGCGATTAAAATCGCGCTGGAGCTCAAGAAGCTGCTGATTGACAACAGCCTGCTAGATGTTTCGCAGTCCGATTTGGAAGTGAATTTGTTTAAGCTTATGGAACGAAGGGGTTATGGGGAAGAGTACATAAATCGGTACAAGATGATGACCAGATTTCACCATCAACGAGTACCATTAGTTATTCTTGTATGTGGAACTGCCTGTGTTGGAAAGTCGACCATTGCTACCCAACTCGCACAAAGACTAAATTTGCCTAATGTCTTGCAGACAGATATGGTATATGAATTGTTGCGCACGTCAACAGATGCACCGTTGACATCTACTCCTGTATGGGAACGAGACTTCAGCTCCTCTGAGGAGTTAATTACTGAATTTTGTAGGGAATGCAGGATTGTCCGTAAAGGATTGGCTGGTGATTTGAAGAAAGCTATGAAAGATGGAAAGCCAATAATAATAGAGGGAATACATTTGGATCCAAGCATTTATTTAATGGAAGATGATAATAAAACACAAGCACAAGAGAAAACTCAGGAGGCAGACCCCGTTGCAGTAGATGGTTCCAAAACACAGATGGAAAACAATTCTCCAAGTATAAGCGGAAGTTCTTCTGAAGTTTGCAACAGTGCTGCACATGTCAGCTGCGAAGAAGGGACACCAACTAACAAGGTGGACGAAGTCTCGGATCGATCTGGAAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_18

Protein Analysis

339

Amino Acids

37.75

Weight (kDa)

5.13

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 166 - 269 1.3e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 608
AccI GTMKAC 2 cut(s) 108, 530
AccII CGCG 1 cut(s) 329
AciI CCGC 2 cut(s) 184, 912
AclI AACGTT 1 cut(s) 93
AclWI GGATC 4 cut(s) 295, 782, 795, 1005
AcsI RAATTY 3 cut(s) 397, 562, 686
AcuI CTGAAG 2 cut(s) 643, 945
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 440, 452, 486
AflIII ACRYGT 1 cut(s) 946
AgsI TTSAA 1 cut(s) 736
AjiI CACGTC 1 cut(s) 612
AluBI AGCT 7 cut(s) 337, 346, 409, 663, 743, 954, 1011
AluI AGCT 7 cut(s) 337, 346, 409, 663, 743, 954, 1011
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 5 cut(s) 11, 61, 160, 648, 997
AlwI GGATC 4 cut(s) 295, 782, 795, 1005
ApeKI GCWGC 3 cut(s) 346, 941, 954
ApoI RAATTY 3 cut(s) 397, 562, 686
Asp700I GAANNNNTTC 1 cut(s) 919
AspLEI GCGC 2 cut(s) 331, 609
AsuHPI GGTGA 4 cut(s) 229, 245, 464, 740
BamHI GGATCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 333, 928, 941
BccI CCATC 5 cut(s) 176, 483, 746, 863, 880
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BcoDI GTCTC 5 cut(s) 11, 61, 160, 648, 997
BfaI CTAG 1 cut(s) 368
BisI GCNGC 3 cut(s) 347, 942, 955
BlsI GCNGC 3 cut(s) 348, 943, 956
BmgBI CACGTC 1 cut(s) 612
BmiI GGNNCC 2 cut(s) 789, 874
BmsI GCATC 1 cut(s) 610
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 43, 323
Bsa29I ATCGAT 1 cut(s) 1000
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 153
BsaXI ACNNNNNCTCC 6 cut(s) 179, 209, 622, 647, 652, 677
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 323
BseCI ATCGAT 1 cut(s) 1000
BseJI GATNNNNATC 1 cut(s) 323
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 3 cut(s) 289, 660, 857
BseRI GAGGAG 2 cut(s) 655, 686
BseXI GCAGC 3 cut(s) 333, 928, 941
BseYI CCCAGC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 1000
BsiHKAI GWGCWC 1 cut(s) 339
BslFI GGGAC 2 cut(s) 166, 979
BsmAI GTCTC 5 cut(s) 11, 61, 160, 648, 997
BsmBI CGTCTC 2 cut(s) 61, 160
BsmFI GGGAC 2 cut(s) 166, 979
BsmI GAATGC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 5 cut(s) 244, 300, 787, 997, 1001
BspACI CCGC 2 cut(s) 184, 912
BspCNI CTCAG 3 cut(s) 288, 661, 856
BspDI ATCGAT 1 cut(s) 1000
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 2 cut(s) 789, 874
BspPI GGATC 4 cut(s) 295, 782, 795, 1005
BsrDI GCAATG 1 cut(s) 534
BssMI GATC 5 cut(s) 244, 300, 787, 997, 1001
BssNI GRCGYC 1 cut(s) 153
Bst4CI ACNGT 3 cut(s) 292, 627, 938
Bst6I CTCTTC 2 cut(s) 163, 429
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 4 cut(s) 275, 297, 669, 843
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 2 cut(s) 331, 609
BstKTI GATC 5 cut(s) 247, 303, 790, 1000, 1004
BstMAI GTCTC 5 cut(s) 11, 61, 160, 648, 997
BstMBI GATC 5 cut(s) 244, 300, 787, 997, 1001
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 950
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 3 cut(s) 333, 928, 941
BstX2I RGATCY 2 cut(s) 300, 787
BstYI RGATCY 2 cut(s) 300, 787
Bsu15I ATCGAT 1 cut(s) 1000
BsuTUI ATCGAT 1 cut(s) 1000
BtrI CACGTC 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 943
Cac8I GCNNGC 1 cut(s) 365
CfoI GCGC 2 cut(s) 331, 609
ClaI ATCGAT 1 cut(s) 1000
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 439, 451, 485
CviAII CATG 1 cut(s) 947
CviQI GTAC 3 cut(s) 439, 451, 485
DdeI CTNAG 4 cut(s) 275, 297, 669, 843
DpnI GATC 5 cut(s) 246, 302, 789, 999, 1003
DpnII GATC 5 cut(s) 244, 300, 787, 997, 1001
Eam1104I CTCTTC 2 cut(s) 163, 429
EarI CTCTTC 2 cut(s) 163, 429
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 643, 945
EcoICRI GAGCTC 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 339
Esp3I CGTCTC 2 cut(s) 61, 160
FaeI CATG 1 cut(s) 950
FaqI GGGAC 2 cut(s) 166, 979
FatI CATG 1 cut(s) 946
FauI CCCGC 1 cut(s) 191
FblI GTMKAC 2 cut(s) 108, 530
Fnu4HI GCNGC 3 cut(s) 347, 942, 955
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 347, 942, 955
FspBI CTAG 1 cut(s) 368
FspI TGCGCA 1 cut(s) 608
GlaI GCGC 2 cut(s) 330, 608
GluI GCNGC 3 cut(s) 347, 942, 955
GsaI CCCAGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 353
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 331, 609
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 1 cut(s) 950
Hin6I GCGC 2 cut(s) 329, 607
HinP1I GCGC 2 cut(s) 329, 607
HincII GTYRAC 5 cut(s) 97, 288, 531, 615, 630
HindII GTYRAC 5 cut(s) 97, 288, 531, 615, 630
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 2 cut(s) 6, 174
HpaI GTTAAC 1 cut(s) 97
HphI GGTGA 4 cut(s) 229, 245, 464, 740
Hpy166II GTNNAC 7 cut(s) 97, 109, 288, 531, 615, 630, 985
Hpy188I TCNGA 5 cut(s) 173, 385, 670, 925, 997
Hpy188III TCNNGA 5 cut(s) 264, 304, 340, 845, 1005
Hpy8I GTNNAC 7 cut(s) 97, 109, 288, 531, 615, 630, 985
Hpy99I CGWCG 3 cut(s) 86, 89, 131
HpyAV CCTTC 3 cut(s) 214, 414, 956
HpyCH4III ACNGT 3 cut(s) 292, 627, 938
HpyCH4IV ACGT 3 cut(s) 93, 105, 611
HpyCH4V TGCA 7 cut(s) 242, 580, 623, 702, 863, 933, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 4 cut(s) 275, 297, 669, 843
HpySE526I ACGT 3 cut(s) 93, 105, 611
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 1 cut(s) 950
HspAI GCGC 2 cut(s) 329, 607
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 5 cut(s) 244, 300, 787, 997, 1001
LmnI GCTCC 2 cut(s) 334, 668
Lsp1109I GCAGC 3 cut(s) 333, 928, 941
LweI GCATC 1 cut(s) 610
MaeI CTAG 1 cut(s) 368
MaeII ACGT 3 cut(s) 93, 105, 611
MaeIII GTNAC 1 cut(s) 292
MalI GATC 5 cut(s) 246, 302, 789, 999, 1003
MboI GATC 5 cut(s) 244, 300, 787, 997, 1001
MboII GAAGA 6 cut(s) 150, 446, 748, 821, 912, 971
MflI RGATCY 2 cut(s) 300, 787
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 7 cut(s) 307, 397, 562, 599, 678, 686, 895
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 5 cut(s) 205, 664, 676, 766, 841
MroXI GAANNNNTTC 1 cut(s) 919
MseI TTAA 5 cut(s) 96, 321, 405, 677, 803
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 954
Mva1269I GAATGC 1 cut(s) 704
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 343
NdeII GATC 5 cut(s) 244, 300, 787, 997, 1001
NlaIII CATG 1 cut(s) 950
NlaIV GGNNCC 2 cut(s) 789, 874
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 608
NspI RCATGY 1 cut(s) 950
PciI ACATGT 1 cut(s) 946
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 90
PctI GAATGC 1 cut(s) 704
PdmI GAANNNNTTC 1 cut(s) 919
PfeI GAWTC 2 cut(s) 6, 174
PflFI GACNNNGTC 2 cut(s) 209, 989
PkrI GCNGC 3 cut(s) 348, 943, 956
PscI ACATGT 1 cut(s) 946
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 789, 874
PsuI RGATCY 2 cut(s) 300, 787
PsyI GACNNNGTC 2 cut(s) 209, 989
PvuII CAGCTG 1 cut(s) 954
RsaI GTAC 3 cut(s) 440, 452, 486
RsaNI GTAC 3 cut(s) 439, 451, 485
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 529
SaqAI TTAA 5 cut(s) 96, 321, 405, 677, 803
SatI GCNGC 3 cut(s) 347, 942, 955
Sau3AI GATC 5 cut(s) 244, 300, 787, 997, 1001
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 1 cut(s) 610
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 58, 338
SmoI CTYRAG 2 cut(s) 58, 338
Sse9I AATT 7 cut(s) 307, 397, 562, 599, 678, 686, 895
SsiI CCGC 2 cut(s) 184, 912
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 339
TaaI ACNGT 3 cut(s) 292, 627, 938
TaiI ACGT 3 cut(s) 96, 108, 614
TaqI TCGA 5 cut(s) 81, 161, 280, 530, 1000
TasI AATT 7 cut(s) 307, 397, 562, 599, 678, 686, 895
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 2 cut(s) 6, 174
Tru1I TTAA 5 cut(s) 96, 321, 405, 677, 803
Tru9I TTAA 5 cut(s) 96, 321, 405, 677, 803
TscAI CASTG 1 cut(s) 943
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 346, 941, 954
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 3 cut(s) 44, 612, 761
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 1 cut(s) 943
Tth111I GACNNNGTC 2 cut(s) 209, 989
XapI RAATTY 3 cut(s) 397, 562, 686
XceI RCATGY 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 2 cut(s) 108, 530
XmnI GAANNNNTTC 1 cut(s) 919
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.