pycom07g27110

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003829
Physical Location & Seq
Reverse (-)
26909158 .. 26911167
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGAGGTCGCAGGTTTGGATTTGATGGTGCTTCTGGGTATTTCGATGGCATTTTTTATTGGAGTTTTGATCGGATGGTCGTGGAAACCAAAGTGGGCATTCTCTGGCAATGACAAGGACAAACTAGGCTGCCCTGTGCCTAAAGCTTTTGATTTTTCGCAGCCTTCTTCGCCGCCCCGGTCCCCTTTAAAGGGGTTTTCGTCAGCTCCATGCTTGAATTGTTTTGAGTCATTGGTTTCCCAAGGTTGTGAAGCTTGCTGCATGGGTAAGGGAATTCAGAAAACTACTTCTGTATCGCCTCCGGCTACCGAATTCGATGAGGGCAGCAGCACATCGCAGCTGAATGACGAGAAGCCGAAAGTAGTGACTGAGGAGGATTTAGAGGTTTTATCCGAGCTTGTAGATATGAAAGATGGAGGTCCTCCATGGATACAGATGATGGATCGCTCCACCCCAACTATGACCTACCAAGGCTGGCGTAGGGATCCAAAGGTCGGTCCTCCTCAATATCGTAGCAGAACTGTCTATGAGGATGCAACGCCGGAATTAGTGAGGGACTTCTTCTGGGATGACGAGTTTCGACTAAGGTGGGATGACATGCTTAGTTATGCGAAAACCATAGAAGAATGCCCGACCACAGGAACCATGGTGACGCAGTGGATTAAGAAGTTCCCGTTTTTCTGTAAAGACAGAGAATACATCATAGGTCGTCGAATTTGGGAATCTGGGAGGTTGTATTATTGCGTGACAAAGGGAGTGCCCTATGCTTCTGTTCCAAGACATGACAAGCAAGTACGCGTAGATCTGTACTTCTCAAGTTGGTGCATTCAAGCAGTGGAATCAAGAAGGGCTGACGGCCAGCTGACTGCGTGTGAGGTTATATTCTTACATTACGAAGATATGGGACTCCCATGGGAAATTGTAAAACTTGGATTACGAAAAGGGATGTGGGATACAGTCAAGAAGATTGAGGCTGGCTTACGCGCCTACCAGAAGGAAAGAGCAGCAGGATCACCACTTTCCCGGCCAGCCTTTATGGCTCAGATCAACACGAAAATACATCCCGGGTACCTGAAATCCTCAGGAGCCACAGAAGACTCATCACAAACTGAGGTCGTGATTGCATCCAAGAAGCCTGCGGGTAGGCACATACCCAAGCTCTTAATACTTGTGGGGACAATAATCATTGCTTGCACTCTTACTAGGGGACACCTAGGCAGAAAGCTTGCGAAGGTAGGAAAGAAATTGCAAACAAAGAGTGATCGAGATTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

426

Amino Acids

47.8

Weight (kDa)

8.69

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 136 - 330 6.3e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2
Acc65I GGTACC 1 cut(s) 1068
AccB1I GGYRCC 1 cut(s) 1068
AccII CGCG 2 cut(s) 798, 984
AciI CCGC 2 cut(s) 173, 1139
AclWI GGATC 4 cut(s) 450, 479, 492, 1018
AcoI YGGCCR 2 cut(s) 856, 1025
AcsI RAATTY 3 cut(s) 273, 311, 714
AfaI GTAC 3 cut(s) 795, 809, 1070
AfiI CCNNNNNNNGG 4 cut(s) 190, 191, 494, 638
AflIII ACRYGT 1 cut(s) 796
AgsI TTSAA 3 cut(s) 217, 830, 1272
AluBI AGCT 8 cut(s) 146, 206, 254, 340, 397, 862, 1159, 1225
AluI AGCT 8 cut(s) 146, 206, 254, 340, 397, 862, 1159, 1225
AlwI GGATC 4 cut(s) 450, 479, 492, 1018
Ama87I CYCGRG 1 cut(s) 1064
AoxI GGCC 2 cut(s) 856, 1025
ApeKI GCWGC 7 cut(s) 129, 160, 258, 324, 327, 337, 1004
ApoI RAATTY 3 cut(s) 273, 311, 714
Asp718I GGTACC 1 cut(s) 1068
AspA2I CCTAGG 1 cut(s) 1213
AspLEI GCGC 1 cut(s) 986
AspS9I GGNCC 3 cut(s) 180, 419, 497
AsuC2I CCSGG 4 cut(s) 178, 1024, 1065, 1066
AsuHPI GGTGA 2 cut(s) 661, 1005
AvaI CYCGRG 1 cut(s) 1064
AvaII GGWCC 3 cut(s) 180, 419, 497
AvrII CCTAGG 1 cut(s) 1213
AxyI CCTNAGG 1 cut(s) 1081
BaeGI GKGCMC 1 cut(s) 762
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 1101
BbvI GCAGC 7 cut(s) 116, 172, 245, 336, 339, 349, 1016
BccI CCATC 5 cut(s) 19, 40, 69, 407, 433
BceAI ACGGC 1 cut(s) 871
BciVI GTATCC 2 cut(s) 423, 946
BcnI CCSGG 4 cut(s) 178, 1024, 1065, 1066
BfaI CTAG 3 cut(s) 125, 1203, 1214
BfuAI ACCTGC 1 cut(s) 2
BfuI GTATCC 2 cut(s) 423, 946
BglI GCCNNNNNGGC 1 cut(s) 1037
BglII AGATCT 1 cut(s) 802
BisI GCNGC 8 cut(s) 130, 161, 173, 259, 325, 328, 338, 1005
BlnI CCTAGG 1 cut(s) 1213
BlsI GCNGC 8 cut(s) 131, 162, 174, 260, 326, 329, 339, 1006
Bme1390I CCNGG 4 cut(s) 178, 1024, 1065, 1066
Bme18I GGWCC 3 cut(s) 180, 419, 497
BmeT110I CYCGRG 1 cut(s) 1064
BmgT120I GGNCC 3 cut(s) 180, 419, 497
BmiI GGNNCC 5 cut(s) 182, 486, 643, 1070, 1087
BmrFI CCNGG 4 cut(s) 178, 1024, 1065, 1066
BmsI GCATC 2 cut(s) 523, 1133
BpiI GAAGAC 1 cut(s) 1101
BpuEI CTTGAG 1 cut(s) 799
BpuMI CCSGG 4 cut(s) 178, 1024, 1065, 1066
BsaJI CCNNGG 8 cut(s) 176, 241, 425, 469, 645, 911, 1064, 1213
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 191, 494, 638
Bse21I CCTNAGG 1 cut(s) 1081
Bse3DI GCAATG 2 cut(s) 115, 1185
BseDI CCNNGG 8 cut(s) 176, 241, 425, 469, 645, 911, 1064, 1213
BseGI GGATG 7 cut(s) 80, 538, 574, 598, 951, 1060, 1124
BseLI CCNNNNNNNGG 4 cut(s) 190, 191, 494, 638
BseMI GCAATG 2 cut(s) 115, 1185
BseMII CTCAG 4 cut(s) 360, 1055, 1095, 1101
BseRI GAGGAG 2 cut(s) 386, 492
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 7 cut(s) 116, 172, 245, 336, 339, 349, 1016
Bsh1236I CGCG 2 cut(s) 798, 984
BshFI GGCC 2 cut(s) 858, 1027
BshNI GGYRCC 1 cut(s) 1068
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 5 cut(s) 178, 302, 542, 1024, 1065
BslFI GGGAC 5 cut(s) 166, 569, 918, 1189, 1221
BslI CCNNNNNNNGG 4 cut(s) 190, 191, 494, 638
BsmFI GGGAC 5 cut(s) 166, 569, 918, 1189, 1221
BsmI GAATGC 3 cut(s) 98, 632, 825
BsnI GGCC 2 cut(s) 858, 1027
BsoBI CYCGRG 1 cut(s) 1064
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
Bsp19I CCATGG 3 cut(s) 425, 645, 911
BspACI CCGC 2 cut(s) 173, 1139
BspANI GGCC 2 cut(s) 858, 1027
BspCNI CTCAG 4 cut(s) 361, 1054, 1094, 1102
BspFNI CGCG 2 cut(s) 798, 984
BspLI GGNNCC 5 cut(s) 182, 486, 643, 1070, 1087
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 4 cut(s) 450, 479, 492, 1018
BspT107I GGYRCC 1 cut(s) 1068
BsrDI GCAATG 2 cut(s) 115, 1185
BssECI CCNNGG 8 cut(s) 176, 241, 425, 469, 645, 911, 1064, 1213
BssMI GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
BssT1I CCWWGG 6 cut(s) 241, 425, 469, 645, 911, 1213
Bst4CI ACNGT 2 cut(s) 523, 958
BstC8I GCNNGC 8 cut(s) 256, 476, 860, 976, 1029, 1137, 1192, 1227
BstDEI CTNAG 6 cut(s) 369, 584, 602, 1041, 1081, 1110
BstDSI CCRYGG 3 cut(s) 425, 645, 911
BstF5I GGATG 7 cut(s) 80, 538, 574, 598, 951, 1060, 1124
BstFNI CGCG 2 cut(s) 798, 984
BstHHI GCGC 1 cut(s) 986
BstKTI GATC 7 cut(s) 72, 445, 487, 805, 1013, 1047, 1264
BstMBI GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
BstMWI GCNNNNNNNGC 2 cut(s) 169, 1037
BstNSI RCATGY 1 cut(s) 601
BstSCI CCNGG 4 cut(s) 176, 1022, 1063, 1064
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 2 cut(s) 798, 984
BstV1I GCAGC 7 cut(s) 116, 172, 245, 336, 339, 349, 1016
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 2 cut(s) 484, 802
BstYI RGATCY 2 cut(s) 484, 802
Bsu36I CCTNAGG 1 cut(s) 1081
BsuI GTATCC 2 cut(s) 423, 946
BsuRI GGCC 2 cut(s) 858, 1027
BtgI CCRYGG 3 cut(s) 425, 645, 911
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 7 cut(s) 80, 538, 574, 598, 951, 1060, 1124
BtsI GCAGTG 2 cut(s) 662, 840
BtsIMutI CAGTG 2 cut(s) 662, 840
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 8 cut(s) 256, 476, 860, 976, 1029, 1137, 1192, 1227
CfoI GCGC 1 cut(s) 986
Cfr13I GGNCC 3 cut(s) 180, 419, 497
Cfr9I CCCGGG 1 cut(s) 1064
CseI GACGC 1 cut(s) 661
Csp6I GTAC 3 cut(s) 794, 808, 1069
CviAII CATG 7 cut(s) 210, 262, 426, 598, 646, 782, 912
CviQI GTAC 3 cut(s) 794, 808, 1069
DdeI CTNAG 6 cut(s) 369, 584, 602, 1041, 1081, 1110
DpnI GATC 7 cut(s) 71, 444, 486, 804, 1012, 1046, 1263
DpnII GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
DraI TTTAAA 1 cut(s) 189
EaeI YGGCCR 2 cut(s) 856, 1025
Eco130I CCWWGG 6 cut(s) 241, 425, 469, 645, 911, 1213
Eco47I GGWCC 3 cut(s) 180, 419, 497
Eco81I CCTNAGG 1 cut(s) 1081
Eco88I CYCGRG 1 cut(s) 1064
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 273, 311
EcoT14I CCWWGG 6 cut(s) 241, 425, 469, 645, 911, 1213
ErhI CCWWGG 6 cut(s) 241, 425, 469, 645, 911, 1213
FaeI CATG 7 cut(s) 213, 265, 429, 601, 649, 785, 915
FaqI GGGAC 5 cut(s) 166, 569, 918, 1189, 1221
FatI CATG 7 cut(s) 209, 261, 425, 597, 645, 781, 911
FauI CCCGC 1 cut(s) 1132
Fnu4HI GCNGC 8 cut(s) 130, 161, 173, 259, 325, 328, 338, 1005
FokI GGATG 7 cut(s) 87, 545, 581, 605, 958, 1047, 1111
Fsp4HI GCNGC 8 cut(s) 130, 161, 173, 259, 325, 328, 338, 1005
FspBI CTAG 3 cut(s) 125, 1203, 1214
GlaI GCGC 1 cut(s) 985
GluI GCNGC 8 cut(s) 130, 161, 173, 259, 325, 328, 338, 1005
HaeIII GGCC 2 cut(s) 858, 1027
HapII CCGG 5 cut(s) 178, 302, 542, 1024, 1065
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 986
Hin1II CATG 7 cut(s) 213, 265, 429, 601, 649, 785, 915
Hin6I GCGC 1 cut(s) 984
HinP1I GCGC 1 cut(s) 984
HindIII AAGCTT 3 cut(s) 144, 252, 1223
HinfI GANTC 6 cut(s) 227, 722, 839, 906, 1097, 1268
HpaII CCGG 5 cut(s) 178, 302, 542, 1024, 1065
HphI GGTGA 2 cut(s) 661, 1005
Hpy188I TCNGA 4 cut(s) 74, 279, 394, 1044
Hpy188III TCNNGA 5 cut(s) 843, 961, 1083, 1117, 1265
Hpy99I CGWCG 1 cut(s) 714
HpyAV CCTTC 4 cut(s) 174, 840, 988, 1225
HpyCH4III ACNGT 2 cut(s) 523, 958
HpyCH4V TGCA 6 cut(s) 261, 536, 825, 1124, 1194, 1249
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 1037
HpyF3I CTNAG 6 cut(s) 369, 584, 602, 1041, 1081, 1110
Hsp92II CATG 7 cut(s) 213, 265, 429, 601, 649, 785, 915
HspAI GCGC 1 cut(s) 984
KpnI GGTACC 1 cut(s) 1072
Kzo9I GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
LmnI GCTCC 3 cut(s) 211, 452, 1085
Lsp1109I GCAGC 7 cut(s) 116, 172, 245, 336, 339, 349, 1016
LweI GCATC 2 cut(s) 523, 1133
MaeI CTAG 3 cut(s) 125, 1203, 1214
MaeIII GTNAC 3 cut(s) 364, 649, 745
MalI GATC 7 cut(s) 71, 444, 486, 804, 1012, 1046, 1263
MboI GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
MboII GAAGA 6 cut(s) 159, 553, 635, 908, 976, 1106
MflI RGATCY 2 cut(s) 484, 802
MhlI GDGCHC 1 cut(s) 762
MluCI AATT 7 cut(s) 217, 273, 311, 545, 714, 918, 1244
MluI ACGCGT 1 cut(s) 796
MlyI GAGTC 3 cut(s) 236, 900, 1091
MseI TTAA 3 cut(s) 188, 663, 1163
MspA1I CMGCKG 2 cut(s) 340, 862
MspI CCGG 5 cut(s) 178, 302, 542, 1024, 1065
MspR9I CCNGG 4 cut(s) 178, 1024, 1065, 1066
Mva1269I GAATGC 3 cut(s) 98, 632, 825
MvnI CGCG 2 cut(s) 798, 984
MwoI GCNNNNNNNGC 2 cut(s) 169, 1037
NciI CCSGG 4 cut(s) 178, 1024, 1065, 1066
NcoI CCATGG 3 cut(s) 425, 645, 911
NdeII GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
NlaIII CATG 7 cut(s) 213, 265, 429, 601, 649, 785, 915
NlaIV GGNNCC 5 cut(s) 182, 486, 643, 1070, 1087
NmuCI GTSAC 3 cut(s) 364, 649, 745
NspI RCATGY 1 cut(s) 601
PctI GAATGC 3 cut(s) 98, 632, 825
PfeI GAWTC 3 cut(s) 722, 839, 1268
PkrI GCNGC 8 cut(s) 131, 162, 174, 260, 326, 329, 339, 1006
PleI GAGTC 3 cut(s) 235, 900, 1091
PpsI GAGTC 3 cut(s) 235, 900, 1091
PpuMI RGGWCCY 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 419
PspN4I GGNNCC 5 cut(s) 182, 486, 643, 1070, 1087
PspPI GGNCC 3 cut(s) 180, 419, 497
PspPPI RGGWCCY 1 cut(s) 419
PsuI RGATCY 2 cut(s) 484, 802
PvuII CAGCTG 2 cut(s) 340, 862
RsaI GTAC 3 cut(s) 795, 809, 1070
RsaNI GTAC 3 cut(s) 794, 808, 1069
SaqAI TTAA 3 cut(s) 188, 663, 1163
SatI GCNGC 8 cut(s) 130, 161, 173, 259, 325, 328, 338, 1005
Sau3AI GATC 7 cut(s) 69, 442, 484, 802, 1010, 1044, 1261
Sau96I GGNCC 3 cut(s) 180, 419, 497
SchI GAGTC 3 cut(s) 236, 900, 1091
ScrFI CCNGG 4 cut(s) 178, 1024, 1065, 1066
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 2 cut(s) 523, 1133
SinI GGWCC 3 cut(s) 180, 419, 497
SmaI CCCGGG 1 cut(s) 1066
SmlI CTYRAG 1 cut(s) 814
SmoI CTYRAG 1 cut(s) 814
Sse9I AATT 7 cut(s) 217, 273, 311, 545, 714, 918, 1244
SsiI CCGC 2 cut(s) 173, 1139
SspMI CTAG 3 cut(s) 125, 1203, 1214
StyD4I CCNGG 4 cut(s) 176, 1022, 1063, 1064
StyI CCWWGG 6 cut(s) 241, 425, 469, 645, 911, 1213
TaaI ACNGT 2 cut(s) 523, 958
TaqI TCGA 5 cut(s) 44, 315, 580, 712, 1264
TaqII GACCGA 1 cut(s) 485
TasI AATT 7 cut(s) 217, 273, 311, 545, 714, 918, 1244
TatI WGTACW 1 cut(s) 807
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 3 cut(s) 722, 839, 1268
Tru1I TTAA 3 cut(s) 188, 663, 1163
Tru9I TTAA 3 cut(s) 188, 663, 1163
TscAI CASTG 2 cut(s) 662, 840
TseFI GTSAC 3 cut(s) 364, 649, 745
TseI GCWGC 7 cut(s) 129, 160, 258, 324, 327, 337, 1004
Tsp45I GTSAC 3 cut(s) 364, 649, 745
TspDTI ATGAA 1 cut(s) 422
TspMI CCCGGG 1 cut(s) 1064
TspRI CASTG 2 cut(s) 662, 840
VpaK11BI GGWCC 3 cut(s) 180, 419, 497
XapI RAATTY 3 cut(s) 273, 311, 714
XceI RCATGY 1 cut(s) 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XmaI CCCGGG 1 cut(s) 1064
XmaJI CCTAGG 1 cut(s) 1213
XspI CTAG 3 cut(s) 125, 1203, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.