pycom07g27940

Serine threonine-protein phosphatase 4 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002676
Physical Location & Seq
Forward (+)
27467240 .. 27469840
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGGAGATGCCATCAAATGAAAATTCACAGCCCTCAATGAATTCTACCAGCAATGCTGATCATCAACAGGAGGCCGTGCTTCCCGATGGCGTAGATAATCTATGTAATGAGTCGAAGCTGGAGTTTTCTGAAGCAGAAGTTAGAGAAGTGCTAGAAGTTATTGCATCCACTGGGAAATTTTGGCATGATTGGGACAAATTAAAGAGCATGCTATGGTTTCAGTTGACGCAGGTTCTGTTGGAGTATCCTGAGGAAAAAATGGCAAGTGAGCAGCAAATTACTTCTTTAGGAGAAACCTATCCTGAGCTGGTGAAGAGGTTGGATGAAGCTCTTCATAGTTTTACCGAAGGTCCTCCGTTTACCCTTCAGAGGCTTTGCGAGATCCTATTGGATGCAAAAAGAATCTATCCAAATCTCTCGAAGCTTGCTCTTGCATTAGAAAAGAATCTATTGGTGACATCTATGCTGACAGTCTCAACGGATCCGTATCCACAAGCCACAGTGCAAAATTCAGTTGAACCAGACAAAGCAATTGACGAACCTAAAATTCACTCTGATTCAGTGCAAAATGGGGTGGAACCTGTGGTAGGCGGCGATAGGGATGAAGTTATGGCAGAGGTAGAGCAAGCTGATATCGATGATGACATGACCATTGCCATGGAAGCTTTTGAAGATATAGTTGGATCATCAGACGCAAATTCAGTGCAGACCAATAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPP4R2

Protein Analysis

241

Amino Acids

26.76

Weight (kDa)

4.17

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPP4R2 PF09184 44 - 159 8e-14 PPP4R2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 220
AciI CCGC 1 cut(s) 591
AclWI GGATC 4 cut(s) 376, 476, 489, 691
AcsI RAATTY 6 cut(s) 22, 40, 176, 508, 546, 697
AcuI CTGAAG 2 cut(s) 150, 350
AfiI CCNNNNNNNGG 2 cut(s) 369, 587
AgsI TTSAA 2 cut(s) 518, 671
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
AluBI AGCT 6 cut(s) 118, 307, 329, 424, 629, 665
AluI AGCT 6 cut(s) 118, 307, 329, 424, 629, 665
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 4 cut(s) 376, 476, 489, 691
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 1 cut(s) 271
ApoI RAATTY 6 cut(s) 22, 40, 176, 508, 546, 697
Asp700I GAANNNNTTC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 322, 466
AvaII GGWCC 1 cut(s) 350
AxyI CCTNAGG 1 cut(s) 249
BamHI GGATCC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 283
BccI CCATC 2 cut(s) 19, 80
BceAI ACGGC 1 cut(s) 59
BciVI GTATCC 2 cut(s) 255, 498
BclI TGATCA 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 220
BfuI GTATCC 2 cut(s) 255, 498
BisI GCNGC 2 cut(s) 272, 592
BlsI GCNGC 2 cut(s) 273, 593
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 2 cut(s) 483, 579
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 2 cut(s) 173, 382
BmuI ACTGGG 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 636
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 657
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 587
Bse1I ACTGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 249
Bse3DI GCAATG 2 cut(s) 58, 651
Bse8I GATNNNNATC 1 cut(s) 486
BseCI ATCGAT 1 cut(s) 636
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 4 cut(s) 164, 328, 397, 607
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 2 cut(s) 369, 587
BseMI GCAATG 2 cut(s) 58, 651
BseMII CTCAG 2 cut(s) 240, 294
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 283
BshFI GGCC 1 cut(s) 74
BshVI ATCGAT 1 cut(s) 636
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 369, 587
BsmAI GTCTC 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 1 cut(s) 74
Bsp143I GATC 4 cut(s) 58, 381, 481, 683
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 1 cut(s) 591
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 241, 295
BspDI ATCGAT 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 483, 579
BspMI ACCTGC 1 cut(s) 220
BspPI GGATC 4 cut(s) 376, 476, 489, 691
BspQI GCTCTTC 1 cut(s) 336
BsrDI GCAATG 2 cut(s) 58, 651
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 4 cut(s) 58, 381, 481, 683
BssT1I CCWWGG 1 cut(s) 657
Bst4CI ACNGT 2 cut(s) 472, 502
Bst6I CTCTTC 2 cut(s) 308, 336
BstC8I GCNNGC 3 cut(s) 209, 426, 627
BstDEI CTNAG 3 cut(s) 249, 303, 720
BstDSI CCRYGG 1 cut(s) 657
BstENI CCTNNNNNAGG 1 cut(s) 585
BstF5I GGATG 4 cut(s) 164, 328, 397, 607
BstKTI GATC 4 cut(s) 61, 384, 484, 686
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 4 cut(s) 58, 381, 481, 683
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstNSI RCATGY 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 381, 481
BstXI CCANNNNNNTGG 1 cut(s) 658
BstYI RGATCY 2 cut(s) 381, 481
Bsu15I ATCGAT 1 cut(s) 636
Bsu36I CCTNAGG 1 cut(s) 249
BsuI GTATCC 2 cut(s) 255, 498
BsuRI GGCC 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 636
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 4 cut(s) 164, 328, 397, 607
BtsIMutI CAGTG 4 cut(s) 168, 507, 567, 708
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 3 cut(s) 209, 426, 627
CaiI CAGNNNCTG 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 350
ClaI ATCGAT 1 cut(s) 636
CseI GACGC 2 cut(s) 235, 701
CviAII CATG 4 cut(s) 185, 208, 646, 658
DdeI CTNAG 3 cut(s) 249, 303, 720
DpnI GATC 4 cut(s) 60, 383, 483, 685
DpnII GATC 4 cut(s) 58, 381, 481, 683
Eam1104I CTCTTC 2 cut(s) 308, 336
EarI CTCTTC 2 cut(s) 308, 336
Eco130I CCWWGG 1 cut(s) 657
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 2 cut(s) 150, 350
Eco81I CCTNAGG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 585
EcoO109I RGGNCCY 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 40
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 4 cut(s) 188, 211, 649, 661
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 184, 207, 645, 657
FbaI TGATCA 1 cut(s) 58
Fnu4HI GCNGC 2 cut(s) 272, 592
FokI GGATG 4 cut(s) 151, 335, 404, 614
Fsp4HI GCNGC 2 cut(s) 272, 592
FspBI CTAG 1 cut(s) 152
GluI GCNGC 2 cut(s) 272, 592
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 1 cut(s) 74
HgaI GACGC 2 cut(s) 235, 701
Hin1II CATG 4 cut(s) 188, 211, 649, 661
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HindIII AAGCTT 2 cut(s) 422, 663
HinfI GANTC 4 cut(s) 110, 402, 445, 557
HphI GGTGA 2 cut(s) 322, 466
Hpy166II GTNNAC 2 cut(s) 225, 360
Hpy188I TCNGA 4 cut(s) 130, 369, 556, 691
Hpy188III TCNNGA 4 cut(s) 83, 248, 302, 418
Hpy8I GTNNAC 2 cut(s) 225, 360
HpyAV CCTTC 2 cut(s) 341, 374
HpyCH4III ACNGT 2 cut(s) 472, 502
HpyCH4V TGCA 6 cut(s) 164, 395, 434, 505, 565, 706
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 3 cut(s) 249, 303, 720
Hsp92II CATG 4 cut(s) 188, 211, 649, 661
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 4 cut(s) 58, 381, 481, 683
LguI GCTCTTC 1 cut(s) 336
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 2 cut(s) 173, 382
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 454
MalI GATC 4 cut(s) 60, 383, 483, 685
MboI GATC 4 cut(s) 58, 381, 481, 683
MboII GAAGA 3 cut(s) 323, 325, 683
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 2 cut(s) 381, 481
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 3 cut(s) 219, 300, 661
MnlI CCTC 7 cut(s) 43, 64, 244, 309, 363, 363, 610
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 656
MunI CAATTG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 662
NcoI CCATGG 1 cut(s) 657
NdeII GATC 4 cut(s) 58, 381, 481, 683
NlaIII CATG 4 cut(s) 188, 211, 649, 661
NlaIV GGNNCC 2 cut(s) 483, 579
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 211
PaeI GCATGC 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 3 cut(s) 402, 445, 557
PkrI GCNGC 2 cut(s) 273, 593
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 350
Psp5II RGGWCCY 1 cut(s) 350
PspN4I GGNNCC 2 cut(s) 483, 579
PspPI GGNCC 1 cut(s) 350
PspPPI RGGWCCY 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 235
PsuI RGATCY 2 cut(s) 381, 481
RseI CAYNNNNRTG 1 cut(s) 656
SapI GCTCTTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 272, 592
Sau3AI GATC 4 cut(s) 58, 381, 481, 683
Sau96I GGNCC 1 cut(s) 350
SchI GAGTC 1 cut(s) 119
SfaNI GCATC 2 cut(s) 173, 382
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 656
SphI GCATGC 1 cut(s) 211
SsiI CCGC 1 cut(s) 591
SspMI CTAG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 2 cut(s) 472, 502
TaqI TCGA 3 cut(s) 113, 419, 636
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 3 cut(s) 402, 445, 557
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 4 cut(s) 175, 507, 567, 708
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 5 cut(s) 33, 53, 323, 339, 618
TspGWI ACGGA 3 cut(s) 345, 474, 494
TspRI CASTG 4 cut(s) 175, 507, 567, 708
VpaK11BI GGWCC 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 585
XapI RAATTY 6 cut(s) 22, 40, 176, 508, 546, 697
XceI RCATGY 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.