pycom08g04380

Solute carrier family 35 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002838
Physical Location & Seq
Forward (+)
3375277 .. 3378018
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGGGTTGGAAGTACAACGCCGGTTTGGGGTTGATTGGCACAGTTGTGTTCATCTGGGTCGCGTCTGCAGAGGTTACTCAGAGAATTTTCTGTGAGTACAAACAGCCGTTTTCGCTCACGTACCTCGGTGTGTCTCTGATGGCGGTCTATCTACCCATATCGGCATTGAAAGATTGGATCTACAACTTGCTGCACCCAAATTTGGTTAAGAACTTTTACAATGGCATTATCGCGGGAACTTCTGGAGGGCTTGGTATTCCTCTTAGAATAAATGAAATGCTCCACAGTCCAGAAACAGATGTTAGAAGCTGCCTAATTACTGACAAAGATCTTAGTAATACAGAAGAAGGGCGAGCTCTAACGGATAAGAGCAAAGAAGATGAGCCGCGCTTGCTTGAACGGAGTTGTCAAATTAGTCCATGGGAAGTTGCAAAGTTTGGCTTAATTCTTACTCCAGTGTGGTTTATCACAGAGTATCTGTCAAACTCTGCACTGGCAAATACCAGTGTTGCAAGTACAACTGTCCTAACTTCTACGTCGGGGCTCTTTACACTTTTCTTTGGAGCTCTTCTTCATCAGGATTCCATAACTGTTACAAAGATGGTTGCTGTTTTTGTCAGCATGGCTGGTGTTGTCATGACCACGGTTGGAAAAACTTGGGCCCCTGATGAACTGCTGGGCATCTCTGAGATTAGAAGGCACGCTATAATGGGACATATTTTTGGTCTTCTCTCGGCTGCGTCATATGGATTGTTTACAGTGTTACTCAAGAAATTTGCTGGATCAGAAGATAAGGGAGATAAGGCTGATGTGCAGAAGCTTTTCGGATACATTGGTCTCTTCGCTCTGCTTGGTCTGTGGTGGCTCGTGTGGCCACTCAATGCCATCGGGATTGAACCTTCATTTGAGTTTCCACATTCAAAATCTATTGCCGAAGCTGTGCTGATAAATGGCTTTGTCGGGAGTGTTCTTTCGGATTATTTTTGGTGGCTCTTTCCGTGGTTTGGACATCTCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

340

Amino Acids

37.53

Weight (kDa)

5.91

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 154 - 215 7.7e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 62, 233, 388
AciI CCGC 3 cut(s) 143, 233, 386
AclWI GGATC 2 cut(s) 185, 790
AcoI YGGCCR 1 cut(s) 872
AcsI RAATTY 3 cut(s) 84, 199, 773
AfaI GTAC 4 cut(s) 14, 98, 122, 517
AfiI CCNNNNNNNGG 3 cut(s) 27, 202, 1004
AgsI TTSAA 4 cut(s) 169, 398, 896, 921
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 5 cut(s) 309, 356, 566, 820, 938
AluI AGCT 5 cut(s) 309, 356, 566, 820, 938
Alw21I GWGCWC 2 cut(s) 358, 568
Alw26I GTCTC 2 cut(s) 138, 842
AlwI GGATC 2 cut(s) 185, 790
AoxI GGCC 2 cut(s) 660, 872
ApaI GGGCCC 1 cut(s) 664
ApeKI GCWGC 3 cut(s) 190, 309, 737
ApoI RAATTY 3 cut(s) 84, 199, 773
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 660, 661
BaeGI GKGCMC 1 cut(s) 664
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BalI TGGCCA 1 cut(s) 874
BanII GRGCYC 4 cut(s) 358, 546, 568, 664
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 2 cut(s) 358, 568
BbvI GCAGC 3 cut(s) 177, 296, 724
BccI CCATC 3 cut(s) 133, 595, 893
BceAI ACGGC 1 cut(s) 91
BciVI GTATCC 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 138, 842
BfmI CTRYAG 1 cut(s) 66
BfuI GTATCC 1 cut(s) 821
BglII AGATCT 1 cut(s) 328
BisI GCNGC 4 cut(s) 191, 310, 386, 738
BlsI GCNGC 4 cut(s) 192, 311, 387, 739
BmgT120I GGNCC 2 cut(s) 660, 661
BmiI GGNNCC 2 cut(s) 662, 663
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 719
BpmI CTGGAG 2 cut(s) 264, 438
BpuEI CTTGAG 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 4 cut(s) 124, 419, 642, 998
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 202, 1004
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 3 cut(s) 455, 498, 504
BseDI CCNNGG 4 cut(s) 124, 419, 642, 998
BseLI CCNNNNNNNGG 3 cut(s) 27, 202, 1004
BseMII CTCAG 2 cut(s) 92, 678
BseNI ACTGG 3 cut(s) 455, 498, 504
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 3 cut(s) 177, 296, 724
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 3 cut(s) 176, 474, 833
Bsh1236I CGCG 3 cut(s) 62, 233, 388
BshFI GGCC 2 cut(s) 662, 874
BsiHKAI GWGCWC 2 cut(s) 358, 568
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 3 cut(s) 27, 202, 1004
BsmAI GTCTC 2 cut(s) 138, 842
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 2 cut(s) 662, 874
Bso31I GGTCTC 1 cut(s) 842
Bsp120I GGGCCC 1 cut(s) 660
Bsp1286I GDGCHC 4 cut(s) 358, 546, 568, 664
Bsp143I GATC 3 cut(s) 177, 328, 782
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 3 cut(s) 143, 233, 386
BspANI GGCC 2 cut(s) 662, 874
BspCNI CTCAG 2 cut(s) 91, 679
BspFNI CGCG 3 cut(s) 62, 233, 388
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 662, 663
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 2 cut(s) 185, 790
BspQI GCTCTTC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 842
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 3 cut(s) 455, 498, 504
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 4 cut(s) 124, 419, 642, 998
BssMI GATC 3 cut(s) 177, 328, 782
BssSI CACGAG 1 cut(s) 866
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 866
Bst4CI ACNGT 6 cut(s) 43, 287, 523, 592, 646, 760
Bst6I CTCTTC 2 cut(s) 573, 845
BstBAI YACGTR 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 354, 392, 702
BstDEI CTNAG 4 cut(s) 78, 263, 332, 687
BstDSI CCRYGG 3 cut(s) 419, 642, 998
BstFNI CGCG 3 cut(s) 62, 233, 388
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 3 cut(s) 180, 331, 785
BstMAI GTCTC 2 cut(s) 138, 842
BstMBI GATC 3 cut(s) 177, 328, 782
BstMWI GCNNNNNNNGC 3 cut(s) 112, 391, 871
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 664
BstUI CGCG 3 cut(s) 62, 233, 388
BstV1I GCAGC 3 cut(s) 177, 296, 724
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 2 cut(s) 177, 328
BstYI RGATCY 2 cut(s) 177, 328
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 2 cut(s) 662, 874
BtgI CCRYGG 3 cut(s) 419, 642, 998
BtsIMutI CAGTG 4 cut(s) 462, 491, 511, 765
Cac8I GCNNGC 3 cut(s) 354, 392, 702
CciI TCATGA 1 cut(s) 636
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 660, 661
CseI GACGC 2 cut(s) 51, 729
Csp6I GTAC 4 cut(s) 13, 97, 121, 516
CviAII CATG 3 cut(s) 420, 622, 637
CviQI GTAC 4 cut(s) 13, 97, 121, 516
DdeI CTNAG 4 cut(s) 78, 263, 332, 687
DpnI GATC 3 cut(s) 179, 330, 784
DpnII GATC 3 cut(s) 177, 328, 782
EaeI YGGCCR 1 cut(s) 872
Eam1104I CTCTTC 2 cut(s) 573, 845
EarI CTCTTC 2 cut(s) 573, 845
Ecl136II GAGCTC 2 cut(s) 356, 566
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 4 cut(s) 358, 546, 568, 664
Eco31I GGTCTC 1 cut(s) 842
Eco53kI GAGCTC 2 cut(s) 356, 566
EcoICRI GAGCTC 2 cut(s) 356, 566
EcoO109I RGGNCCY 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 4 cut(s) 358, 546, 568, 664
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 3 cut(s) 423, 625, 640
FaiI YATR 9 cut(s) 158, 421, 587, 623, 638, 707, 717, 745, 747
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 726
FatI CATG 3 cut(s) 419, 621, 636
FauI CCCGC 1 cut(s) 226
FauNDI CATATG 1 cut(s) 745
Fnu4HI GCNGC 4 cut(s) 191, 310, 386, 738
FriOI GRGCYC 4 cut(s) 358, 546, 568, 664
Fsp4HI GCNGC 4 cut(s) 191, 310, 386, 738
GlaI GCGC 1 cut(s) 389
GluI GCNGC 4 cut(s) 191, 310, 386, 738
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 2 cut(s) 264, 438
HaeIII GGCC 2 cut(s) 662, 874
HapII CCGG 1 cut(s) 21
HgaI GACGC 2 cut(s) 51, 729
HhaI GCGC 1 cut(s) 390
Hin1II CATG 3 cut(s) 423, 625, 640
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 1 cut(s) 581
HpaII CCGG 1 cut(s) 21
Hpy166II GTNNAC 1 cut(s) 756
Hpy188I TCNGA 6 cut(s) 81, 138, 688, 787, 827, 976
Hpy188III TCNNGA 7 cut(s) 243, 290, 578, 637, 769, 889, 961
Hpy8I GTNNAC 1 cut(s) 756
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 341, 690, 909
HpyCH4III ACNGT 6 cut(s) 43, 287, 523, 592, 646, 760
HpyCH4IV ACGT 2 cut(s) 119, 536
HpyCH4V TGCA 6 cut(s) 68, 193, 431, 491, 512, 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 391, 871
HpyF3I CTNAG 4 cut(s) 78, 263, 332, 687
HpySE526I ACGT 2 cut(s) 119, 536
Hsp92II CATG 3 cut(s) 423, 625, 640
HspAI GCGC 1 cut(s) 388
Kzo9I GATC 3 cut(s) 177, 328, 782
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 285, 563
Lsp1109I GCAGC 3 cut(s) 177, 296, 724
LweI GCATC 1 cut(s) 690
MaeII ACGT 2 cut(s) 119, 536
MaeIII GTNAC 3 cut(s) 73, 592, 762
MalI GATC 3 cut(s) 179, 330, 784
MboI GATC 3 cut(s) 177, 328, 782
MboII GAAGA 7 cut(s) 356, 389, 560, 563, 719, 800, 832
MflI RGATCY 2 cut(s) 177, 328
MhlI GDGCHC 4 cut(s) 358, 546, 568, 664
MlsI TGGCCA 1 cut(s) 874
MluCI AATT 6 cut(s) 84, 199, 315, 411, 444, 773
MluNI TGGCCA 1 cut(s) 874
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 4 cut(s) 64, 134, 239, 270
Mox20I TGGCCA 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 874
MseI TTAA 2 cut(s) 207, 443
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 874
MspI CCGG 1 cut(s) 21
MvnI CGCG 3 cut(s) 62, 233, 388
MwoI GCNNNNNNNGC 3 cut(s) 112, 391, 871
NcoI CCATGG 1 cut(s) 419
NdeI CATATG 1 cut(s) 745
NdeII GATC 3 cut(s) 177, 328, 782
NlaIII CATG 3 cut(s) 423, 625, 640
NlaIV GGNNCC 2 cut(s) 662, 663
NmeAIII GCCGAG 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 1 cut(s) 581
PkrI GCNGC 4 cut(s) 192, 311, 387, 739
Ppu21I YACGTR 1 cut(s) 120
Psp124BI GAGCTC 2 cut(s) 358, 568
PspFI CCCAGC 1 cut(s) 676
PspN4I GGNNCC 2 cut(s) 662, 663
PspOMI GGGCCC 1 cut(s) 660
PspPI GGNCC 2 cut(s) 660, 661
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 2 cut(s) 177, 328
RsaI GTAC 4 cut(s) 14, 98, 122, 517
RsaNI GTAC 4 cut(s) 13, 97, 121, 516
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 2 cut(s) 358, 568
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 207, 443
SatI GCNGC 4 cut(s) 191, 310, 386, 738
Sau3AI GATC 3 cut(s) 177, 328, 782
Sau96I GGNCC 2 cut(s) 660, 661
SduI GDGCHC 4 cut(s) 358, 546, 568, 664
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 6 cut(s) 84, 199, 315, 411, 444, 773
SsiI CCGC 3 cut(s) 143, 233, 386
SstI GAGCTC 2 cut(s) 358, 568
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 6 cut(s) 43, 287, 523, 592, 646, 760
TaiI ACGT 2 cut(s) 122, 539
TasI AATT 6 cut(s) 84, 199, 315, 411, 444, 773
TatI WGTACW 3 cut(s) 12, 96, 515
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 2 cut(s) 207, 443
Tru9I TTAA 2 cut(s) 207, 443
TscAI CASTG 4 cut(s) 462, 498, 511, 765
TseI GCWGC 3 cut(s) 190, 309, 737
TspDTI ATGAA 5 cut(s) 40, 288, 563, 684, 891
TspGWI ACGGA 3 cut(s) 377, 415, 987
TspRI CASTG 4 cut(s) 462, 498, 511, 765
XapI RAATTY 3 cut(s) 84, 199, 773
XmnI GAANNNNTTC 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.