pycom08g09760

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004249
Physical Location & Seq
Forward (+)
8122101 .. 8122873
773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09760.2

Sequence Viewer

Length: 681 bp
ATGGCCGCATACGACTACGACGATGCAGGCACCTACGACGAGACATCGGCGACGCAGCGCCAACCGTCGTCCGGAGCCCCGCCGTCTTCCGACCTCGGTTACGACCCCAACTTCGTCCCCGACTCCGTCAAATCCTTTGTCGTCCACCTCTACCGCTACATCCGCGAGAAGAATGTCTACGAGATCCACCAGATGTACGAGACCTCCTTCCAGACCCTCTCCGATCGCCTCTTCAAGGACACCCCCTGGCCATCCGTCGATGCCGTCGCCGACTACGTCGACAACGACCACGTATTCTGCCTCCTCTACCGCGAGATGTGGTTCCGCCACCTCTACGCCCGCCTGTCCCCGACGCTGAAGCAGCGAATCGACTCCTGGGACAATTACTGCAGCCTCTTCCAGGTCGTCCTCCACGGCGTCGTTAATATGCAGCTGCCGAACCAGTGGCTTTGGGACATGGTCGATGAGTTCGTCTACCAGTTCCAGAGCTTCTGTCAGTACAGGGCTAAGATGAAGAACAAGAACGAGCAGGAGATTGCTCTTCTCAGGCAATTTGATCAAGCTTGGAATGTGTATGGTGTGCTCAATTTCTTGCAAGCTCTGGTTGAGAAGTCGATGATCATTCAGATTCTCGAGCAAGAGAAGGAAGGGCTCGAGCAGTTTACTGCCAATGATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Paf67

Protein Analysis

227

Amino Acids

26.7

Weight (kDa)

4.7

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AccI GTMKAC 3 cut(s) 177, 279, 474
AccII CGCG 2 cut(s) 165, 312
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 7 cut(s) 6, 80, 154, 163, 310, 325, 340
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 2 cut(s) 3, 248
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 2 cut(s) 197, 500
AfiI CCNNNNNNNGG 4 cut(s) 71, 235, 400, 675
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 3 cut(s) 245, 374, 399
AluBI AGCT 4 cut(s) 433, 489, 563, 599
AluI AGCT 4 cut(s) 433, 489, 563, 599
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 35, 194
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 2 cut(s) 632, 653
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 2 cut(s) 3, 248
ApeKI GCWGC 5 cut(s) 55, 361, 390, 430, 433
ArsI GACNNNNNNTTYG 4 cut(s) 95, 123, 127, 155
AspLEI GCGC 1 cut(s) 60
AvaI CYCGRG 2 cut(s) 632, 653
BalI TGGCCA 1 cut(s) 250
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 2 cut(s) 79, 654
BarI GAAGNNNNNNTAC 2 cut(s) 161, 193
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 5 cut(s) 67, 373, 402, 420, 442
BccI CCATC 2 cut(s) 259, 668
BceAI ACGGC 3 cut(s) 67, 248, 430
BciT130I CCWGG 3 cut(s) 247, 376, 401
BclI TGATCA 2 cut(s) 556, 618
BcoDI GTCTC 2 cut(s) 35, 194
BfmI CTRYAG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 6 cut(s) 6, 56, 362, 391, 431, 434
BlsI GCNGC 6 cut(s) 7, 57, 363, 392, 432, 435
Bme1390I CCNGG 3 cut(s) 247, 376, 401
BmeT110I CYCGRG 2 cut(s) 632, 653
BmiI GGNNCC 3 cut(s) 31, 76, 323
BmrFI CCNGG 3 cut(s) 247, 376, 401
BmsI GCATC 2 cut(s) 13, 250
BpiI GAAGAC 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 94, 245, 375, 412
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 235, 400, 675
Bse1I ACTGG 2 cut(s) 442, 478
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 3 cut(s) 247, 376, 401
BseDI CCNNGG 4 cut(s) 94, 245, 375, 412
BseGI GGATG 2 cut(s) 159, 251
BseLI CCNNNNNNNGG 4 cut(s) 71, 235, 400, 675
BseMII CTCAG 1 cut(s) 559
BseNI ACTGG 2 cut(s) 442, 478
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 5 cut(s) 67, 373, 402, 420, 442
Bsh1236I CGCG 2 cut(s) 165, 312
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 5, 250
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 2 cut(s) 632, 653
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 4 cut(s) 101, 331, 392, 467
BslI CCNNNNNNNGG 4 cut(s) 71, 235, 400, 675
BsmAI GTCTC 2 cut(s) 35, 194
BsmFI GGGAC 4 cut(s) 101, 331, 392, 467
BsnI GGCC 2 cut(s) 5, 250
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 2 cut(s) 632, 653
Bsp1286I GDGCHC 3 cut(s) 79, 585, 654
Bsp13I TCCGGA 1 cut(s) 71
Bsp143I GATC 4 cut(s) 183, 223, 556, 618
BspACI CCGC 7 cut(s) 6, 80, 154, 163, 310, 325, 340
BspANI GGCC 2 cut(s) 5, 250
BspCNI CTCAG 1 cut(s) 558
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 2 cut(s) 165, 312
BspLI GGNNCC 3 cut(s) 31, 76, 323
BspMAI CTGCAG 1 cut(s) 392
BspPI GGATC 1 cut(s) 178
BspQI GCTCTTC 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 194
BsrI ACTGG 2 cut(s) 442, 478
BssECI CCNNGG 4 cut(s) 94, 245, 375, 412
BssMI GATC 4 cut(s) 183, 223, 556, 618
BssNI GRCGYC 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 247, 376, 401
Bst4CI ACNGT 1 cut(s) 66
Bst6I CTCTTC 3 cut(s) 236, 401, 546
BstACI GRCGYC 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 3 cut(s) 28, 340, 597
BstDEI CTNAG 2 cut(s) 507, 545
BstDSI CCRYGG 1 cut(s) 412
BstENI CCTNNNNNAGG 2 cut(s) 233, 398
BstF5I GGATG 2 cut(s) 159, 251
BstFNI CGCG 2 cut(s) 165, 312
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 4 cut(s) 186, 226, 559, 621
BstMAI GTCTC 2 cut(s) 35, 194
BstMBI GATC 4 cut(s) 183, 223, 556, 618
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 162, 361
BstNI CCWGG 3 cut(s) 247, 376, 401
BstSCI CCNGG 3 cut(s) 245, 374, 399
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 2 cut(s) 165, 312
BstV1I GCAGC 5 cut(s) 67, 373, 402, 420, 442
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 2 cut(s) 5, 250
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 2 cut(s) 159, 251
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 28, 340, 597
CfoI GCGC 1 cut(s) 60
CseI GACGC 3 cut(s) 61, 361, 406
Csp6I GTAC 2 cut(s) 196, 499
CviAII CATG 1 cut(s) 457
CviQI GTAC 2 cut(s) 196, 499
DdeI CTNAG 2 cut(s) 507, 545
DpnI GATC 4 cut(s) 185, 225, 558, 620
DpnII GATC 4 cut(s) 183, 223, 556, 618
EaeI YGGCCR 2 cut(s) 3, 248
Eam1104I CTCTTC 3 cut(s) 236, 401, 546
EarI CTCTTC 3 cut(s) 236, 401, 546
EciI GGCGGA 1 cut(s) 314
Eco24I GRGCYC 2 cut(s) 79, 654
Eco31I GGTCTC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 377
Eco88I CYCGRG 2 cut(s) 632, 653
EcoNI CCTNNNNNAGG 2 cut(s) 233, 398
EcoRII CCWGG 3 cut(s) 245, 374, 399
EcoT38I GRGCYC 2 cut(s) 79, 654
FaeI CATG 1 cut(s) 460
FaiI YATR 4 cut(s) 10, 428, 458, 576
FaqI GGGAC 4 cut(s) 101, 331, 392, 467
FatI CATG 1 cut(s) 456
FauI CCCGC 2 cut(s) 87, 347
FbaI TGATCA 2 cut(s) 556, 618
FblI GTMKAC 3 cut(s) 177, 279, 474
Fnu4HI GCNGC 6 cut(s) 6, 56, 362, 391, 431, 434
FokI GGATG 2 cut(s) 146, 238
FriOI GRGCYC 2 cut(s) 79, 654
Fsp4HI GCNGC 6 cut(s) 6, 56, 362, 391, 431, 434
GlaI GCGC 1 cut(s) 59
GluI GCNGC 6 cut(s) 6, 56, 362, 391, 431, 434
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 2 cut(s) 5, 250
HapII CCGG 1 cut(s) 72
HgaI GACGC 3 cut(s) 61, 361, 406
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 1 cut(s) 460
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 4 cut(s) 122, 366, 371, 628
HpaII CCGG 1 cut(s) 72
Hpy166II GTNNAC 5 cut(s) 145, 178, 280, 475, 663
Hpy188I TCNGA 3 cut(s) 91, 223, 627
Hpy188III TCNNGA 4 cut(s) 72, 211, 484, 632
Hpy8I GTNNAC 5 cut(s) 145, 178, 280, 475, 663
Hpy99I CGWCG 9 cut(s) 23, 41, 55, 70, 260, 269, 281, 355, 422
HpyAV CCTTC 3 cut(s) 217, 637, 641
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4IV ACGT 2 cut(s) 276, 291
HpyCH4V TGCA 4 cut(s) 26, 390, 430, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 361
HpyF3I CTNAG 2 cut(s) 507, 545
HpySE526I ACGT 2 cut(s) 276, 291
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 1 cut(s) 460
HspAI GCGC 1 cut(s) 58
Kpn2I TCCGGA 1 cut(s) 71
Ksp22I TGATCA 2 cut(s) 556, 618
Kzo9I GATC 4 cut(s) 183, 223, 556, 618
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 5 cut(s) 67, 373, 402, 420, 442
LweI GCATC 2 cut(s) 13, 250
MaeII ACGT 2 cut(s) 276, 291
MaeIII GTNAC 1 cut(s) 98
MalI GATC 4 cut(s) 185, 225, 558, 620
MboI GATC 4 cut(s) 183, 223, 556, 618
MboII GAAGA 6 cut(s) 78, 181, 223, 388, 526, 533
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 3 cut(s) 79, 585, 654
MlsI TGGCCA 1 cut(s) 250
MluCI AATT 3 cut(s) 382, 551, 586
MluNI TGGCCA 1 cut(s) 250
MlyI GAGTC 2 cut(s) 116, 365
MmeI TCCRAC 1 cut(s) 114
Mox20I TGGCCA 1 cut(s) 250
MroI TCCGGA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 250
MseI TTAA 1 cut(s) 423
Msp20I TGGCCA 1 cut(s) 250
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 3 cut(s) 247, 376, 401
MvaI CCWGG 3 cut(s) 247, 376, 401
MvnI CGCG 2 cut(s) 165, 312
MwoI GCNNNNNNNGC 2 cut(s) 162, 361
NdeII GATC 4 cut(s) 183, 223, 556, 618
NlaIII CATG 1 cut(s) 460
NlaIV GGNNCC 3 cut(s) 31, 76, 323
PaeR7I CTCGAG 2 cut(s) 632, 653
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 273, 282, 468
PfeI GAWTC 2 cut(s) 366, 628
PflFI GACNNNGTC 3 cut(s) 125, 275, 458
PkrI GCNGC 6 cut(s) 7, 57, 363, 392, 432, 435
Ple19I CGATCG 1 cut(s) 226
PleI GAGTC 2 cut(s) 116, 365
PpsI GAGTC 2 cut(s) 116, 365
Ppu21I YACGTR 1 cut(s) 292
Psp6I CCWGG 3 cut(s) 245, 374, 399
PspGI CCWGG 3 cut(s) 245, 374, 399
PspN4I GGNNCC 3 cut(s) 31, 76, 323
PspXI VCTCGAGB 1 cut(s) 653
PstI CTGCAG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 183
PsyI GACNNNGTC 3 cut(s) 125, 275, 458
PvuI CGATCG 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 433
RsaI GTAC 2 cut(s) 197, 500
RsaNI GTAC 2 cut(s) 196, 499
SalI GTCGAC 1 cut(s) 278
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 6 cut(s) 6, 56, 362, 391, 431, 434
Sau3AI GATC 4 cut(s) 183, 223, 556, 618
SchI GAGTC 2 cut(s) 116, 365
ScrFI CCNGG 3 cut(s) 247, 376, 401
SduI GDGCHC 3 cut(s) 79, 585, 654
SfaNI GCATC 2 cut(s) 13, 250
SfcI CTRYAG 1 cut(s) 388
Sfr274I CTCGAG 2 cut(s) 632, 653
SlaI CTCGAG 2 cut(s) 632, 653
SmlI CTYRAG 2 cut(s) 632, 653
SmoI CTYRAG 2 cut(s) 632, 653
Sse9I AATT 3 cut(s) 382, 551, 586
SsiI CCGC 7 cut(s) 6, 80, 154, 163, 310, 325, 340
StyD4I CCNGG 3 cut(s) 245, 374, 399
TaaI ACNGT 1 cut(s) 66
TaiI ACGT 2 cut(s) 279, 294
TaqI TCGA 7 cut(s) 258, 279, 369, 462, 614, 633, 654
TasI AATT 3 cut(s) 382, 551, 586
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 366, 628
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 449
TseI GCWGC 5 cut(s) 55, 361, 390, 430, 433
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 2 cut(s) 115, 244
TspRI CASTG 1 cut(s) 449
Tth111I GACNNNGTC 3 cut(s) 125, 275, 458
XagI CCTNNNNNAGG 2 cut(s) 233, 398
XhoI CTCGAG 2 cut(s) 632, 653
XmiI GTMKAC 3 cut(s) 177, 279, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.