Rorug07G0129500

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000683
Physical Location & Seq
Reverse (-)
10254714 .. 10259172
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0129500.1

Sequence Viewer

Length: 1566 bp
ATGGCCTCTCAATCCTCGGAGGACAAACCAGAAAACCCAGTAGGTCTTGCTGAGGCTCAGCAAGATGTTGCAGCTCAGCCTCCTAAGCAAACTTCAACCCCATCGGTGTTTGTGAATTCCGAACCAATGAGAGAGGACCAAGTGCAAAATGCTATGAAGTTTCTTTCGCACCCAAAAGTTAAGGGCTCTCCTGTCATCTACAGGCGCTCTTTTCTTGAGAAGAAGGGGCTTACGAAGGAGGAGATTGATGAGGCGTTTCGGCGTGTGCCTGACCCACCTCCAAGTGCACAGGCAACTACTGCAAATCAAGCTGATGGACAAGCGAAAACAACAAACATTCAGGCACAAAGTTCAAATCAAACTTTGCAGCCTGCTAGTTCTACTGCCCCGGTGGGTACCTTAGCTCGGTATAGATTCCACTGGTCTCATGCCATTCTTGCTGTAGGATTACTGGCAGCTTCAGGTGCTGGAACTGCTTTACTTATTAAGAATGCTATTATTCCAAGGTTGAGGTCTTGGGTACGAAAGGTTGTATTGGAGGATGACAATGACATTGAGAAGAAAACTGACACGAAACCCAGTTTAGCAGAAGAAGCTGCAGCAGCGGCTAAATCAGCTGCAGCTGCAGCAGCAGATGTGGCAAAAGCAAGCCAAGAAATGCTGAATTCTAAGACTGAAGAGAGGAAATACTTTGGGGAACTTATGAGTTTGTTAGATGTGCAAGTGCAAGAAATGAAGTCAATGAGTAATTCCATACGGAAATTGGAAGGAGAAACGAGGGCCTCAAGAGCCTCTCTTGTTGATCACGAAGATAGTCGACTCACAGTCACTAAGTCAAAGCAACAATATGTGAATGGCAAGGCAGAGTACGACATGCACTCAGTGCAATCTTTCTCAGCTCCTGCATCTGTGGAACCATCTGCTGCACCTCACTCAAAGTCTTATATGGAGATCATGGCCATGGTACAAAGAGGGGAGAAACCTCCTAATGTCAGAGAAATTGATGATTTACCGCCCAATCCTAATCAGCAGCCATCAAATCCTCGCTTGGTTCCTAGAGCCAAGCCTTGGGAGCTTGGTCAGACCCAAAACAACTCAAGCCAAGTATATCAGTCCCATACAAGTAGCGAAGCCTTCAGTTCGAGCACGCAGGAGAATGGGCTCAATTATTTAAACGGTGACAGTTCAGTGCCTTGGTGGCAGCGGAAAACTCCCAGTATCACAGAGATAGAAAATGAAGATGAAGTCAAGGCAGCTAGTTCGCAAGTAGTTAAGCAGCCGGTTCAGCGTGCATGGGTTCCTCCCCAGCCACCCCCAGTTGCAATGCCAGAAGCAGCTGAAGCCATCCGACGGCCAAAACCATCAGTGCAGAGAGAATCTCTGGGTAATGATCAGGCCGTAGCACGTTCTTCATCAGATGTGACTGATGAGTTGCAGAGGGTAACAAAAATATCCGAGTCTGGAGGTGCATTGGAGATGAACAATGGGAATGCTGCAGAAATCAGTAATGGGAATTCATATCTGAACTCTACCGAGATACATGAAGAGCAAGAAAACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Paf67

Protein Analysis

521

Amino Acids

56.93

Weight (kDa)

5.54

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex14_N PF04695 44 - 88 4.6e-13 Pex14 N-terminal domain
PEX14-like_2nd PF23020 138 - 256 6e-58 Peroxisomal membrane protein PEX14-like, central
KPWE_dom PF17733 310 - 358 8.3e-16 PEX14-like KPWE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 1068
AccI GTMKAC 1 cut(s) 817
AciI CCGC 3 cut(s) 605, 1013, 1204
AcoI YGGCCR 2 cut(s) 957, 1352
AcsI RAATTY 3 cut(s) 115, 664, 1513
AcuI CTGAAG 4 cut(s) 444, 696, 1120, 1359
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 4 cut(s) 397, 522, 869, 966
AfiI CCNNNNNNNGG 3 cut(s) 405, 1068, 1350
AgsI TTSAA 2 cut(s) 96, 354
AhdI GACNNNNNGTC 1 cut(s) 824
Alw21I GWGCWC 2 cut(s) 289, 1148
Alw26I GTCTC 1 cut(s) 429
Alw44I GTGCAC 1 cut(s) 285
AlwNI CAGNNNCTG 2 cut(s) 467, 902
AoxI GGCC 5 cut(s) 3, 780, 957, 1352, 1395
ApaLI GTGCAC 1 cut(s) 285
ApoI RAATTY 3 cut(s) 115, 664, 1513
Asp718I GGTACC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 136, 780
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 1190
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 289
BalI TGGCCA 1 cut(s) 959
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 188, 1164
Bbv12I GWGCWC 2 cut(s) 289, 1148
BbvCI CCTCAGC 1 cut(s) 51
BccI CCATC 6 cut(s) 109, 308, 925, 1042, 1352, 1369
BceAI ACGGC 2 cut(s) 1367, 1382
BcgI CGANNNNNNTGC 2 cut(s) 1242, 1276
BclI TGATCA 2 cut(s) 802, 1390
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 3 cut(s) 375, 1056, 1257
BfmI CTRYAG 6 cut(s) 199, 441, 597, 618, 624, 1494
BfoI RGCGCY 1 cut(s) 208
BglI GCCNNNNNGGC 1 cut(s) 1198
BlpI GCTNAGC 2 cut(s) 57, 75
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 136
BmeRI GACNNNNNGTC 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 136, 780
BmiI GGNNCC 4 cut(s) 397, 915, 1053, 1299
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 4 cut(s) 32, 573, 1209, 1310
BmsI GCATC 1 cut(s) 914
BmuI ACTGGG 4 cut(s) 32, 573, 1209, 1310
BplI GAGNNNNNCTC 2 cut(s) 1363, 1395
BpmI CTGGAG 1 cut(s) 1482
Bpu10I CCTNAGC 3 cut(s) 51, 84, 400
Bpu1102I GCTNAGC 2 cut(s) 57, 75
BpuEI CTTGAG 3 cut(s) 236, 769, 1081
BpuMI CCSGG 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 6 cut(s) 15, 387, 503, 960, 1067, 1193
Bsc4I CCNNNNNNNGG 3 cut(s) 405, 1068, 1350
Bse118I RCCGGY 1 cut(s) 1279
Bse1I ACTGG 6 cut(s) 38, 425, 456, 579, 1215, 1316
Bse3DI GCAATG 1 cut(s) 1329
BseDI CCNNGG 6 cut(s) 15, 387, 503, 960, 1067, 1193
BseGI GGATG 2 cut(s) 547, 1344
BseLI CCNNNNNNNGG 3 cut(s) 405, 1068, 1350
BseMI GCAATG 1 cut(s) 1329
BseMII CTCAG 5 cut(s) 42, 71, 89, 894, 909
BseNI ACTGG 6 cut(s) 38, 425, 456, 579, 1215, 1316
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 1305
BsgI GTGCAG 2 cut(s) 909, 1388
BshFI GGCC 5 cut(s) 5, 782, 959, 1354, 1397
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 2 cut(s) 289, 1148
BsiSI CCGG 2 cut(s) 389, 1280
BslFI GGGAC 1 cut(s) 1099
BslI CCNNNNNNNGG 3 cut(s) 405, 1068, 1350
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 1099
BsmI GAATGC 2 cut(s) 496, 1495
BsnI GGCC 5 cut(s) 5, 782, 959, 1354, 1397
Bso31I GGTCTC 1 cut(s) 429
Bsp1286I GDGCHC 4 cut(s) 188, 289, 1148, 1164
Bsp143I GATC 3 cut(s) 802, 951, 1390
Bsp1720I GCTNAGC 2 cut(s) 57, 75
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 3 cut(s) 605, 1013, 1204
BspANI GGCC 5 cut(s) 5, 782, 959, 1354, 1397
BspCNI CTCAG 5 cut(s) 43, 70, 88, 893, 908
BspLI GGNNCC 4 cut(s) 397, 915, 1053, 1299
BspMAI CTGCAG 4 cut(s) 601, 622, 628, 1498
BspQI GCTCTTC 1 cut(s) 1539
BspT107I GGYRCC 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 1329
BsrFI RCCGGY 1 cut(s) 1279
BsrI ACTGG 6 cut(s) 38, 425, 456, 579, 1215, 1316
BssAI RCCGGY 1 cut(s) 1279
BssECI CCNNGG 6 cut(s) 15, 387, 503, 960, 1067, 1193
BssMI GATC 3 cut(s) 802, 951, 1390
BssT1I CCWWGG 4 cut(s) 503, 960, 1067, 1193
Bst4CI ACNGT 3 cut(s) 826, 1178, 1184
Bst6I CTCTTC 2 cut(s) 672, 1539
BstAPI GCANNNNNTGC 2 cut(s) 299, 883
BstC8I GCNNGC 4 cut(s) 372, 649, 1148, 1290
BstDEI CTNAG 9 cut(s) 51, 57, 75, 84, 400, 669, 831, 880, 895
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 2 cut(s) 547, 1344
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 805, 954, 1393
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 3 cut(s) 802, 951, 1390
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 6 cut(s) 199, 441, 597, 618, 624, 1494
BstSLI GKGCMC 1 cut(s) 289
BsuRI GGCC 5 cut(s) 5, 782, 959, 1354, 1397
BtgI CCRYGG 1 cut(s) 960
BtsCI GGATG 2 cut(s) 547, 1344
BtsIMutI CAGTG 4 cut(s) 418, 888, 1194, 1371
Cac8I GCNNGC 4 cut(s) 372, 649, 1148, 1290
CaiI CAGNNNCTG 2 cut(s) 467, 902
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 1279
Cfr13I GGNCC 2 cut(s) 136, 780
Csp6I GTAC 4 cut(s) 396, 521, 868, 965
CviAII CATG 6 cut(s) 428, 874, 955, 961, 1293, 1541
CviQI GTAC 4 cut(s) 396, 521, 868, 965
DdeI CTNAG 9 cut(s) 51, 57, 75, 84, 400, 669, 831, 880, 895
DpnI GATC 3 cut(s) 804, 953, 1392
DpnII GATC 3 cut(s) 802, 951, 1390
DraI TTTAAA 1 cut(s) 1173
DraIII CACNNNGTG 1 cut(s) 883
DriI GACNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 2 cut(s) 957, 1352
Eam1104I CTCTTC 2 cut(s) 672, 1539
Eam1105I GACNNNNNGTC 1 cut(s) 824
EarI CTCTTC 2 cut(s) 672, 1539
Eco130I CCWWGG 4 cut(s) 503, 960, 1067, 1193
Eco24I GRGCYC 2 cut(s) 188, 1164
Eco31I GGTCTC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 4 cut(s) 444, 696, 1120, 1359
EcoO109I RGGNCCY 1 cut(s) 780
EcoRI GAATTC 3 cut(s) 115, 664, 1513
EcoT14I CCWWGG 4 cut(s) 503, 960, 1067, 1193
EcoT38I GRGCYC 2 cut(s) 188, 1164
ErhI CCWWGG 4 cut(s) 503, 960, 1067, 1193
FaeI CATG 6 cut(s) 431, 877, 958, 964, 1296, 1544
FaqI GGGAC 1 cut(s) 1099
FatI CATG 6 cut(s) 427, 873, 954, 960, 1292, 1540
FbaI TGATCA 2 cut(s) 802, 1390
FblI GTMKAC 1 cut(s) 817
FokI GGATG 2 cut(s) 554, 1331
FriOI GRGCYC 2 cut(s) 188, 1164
FspBI CTAG 3 cut(s) 375, 1056, 1257
GlaI GCGC 1 cut(s) 206
GsaI CCCAGC 1 cut(s) 1309
GsuI CTGGAG 1 cut(s) 1482
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 5 cut(s) 5, 782, 959, 1354, 1397
HapII CCGG 2 cut(s) 389, 1280
HhaI GCGC 1 cut(s) 207
Hin1II CATG 6 cut(s) 431, 877, 958, 964, 1296, 1544
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 818
HindII GTYRAC 1 cut(s) 818
HinfI GANTC 4 cut(s) 414, 819, 1376, 1457
HpaII CCGG 2 cut(s) 389, 1280
HphI GGTGA 1 cut(s) 1190
Hpy166II GTNNAC 2 cut(s) 287, 818
Hpy188I TCNGA 8 cut(s) 19, 121, 995, 1083, 1349, 1417, 1456, 1524
Hpy188III TCNNGA 4 cut(s) 215, 786, 806, 1461
Hpy8I GTNNAC 2 cut(s) 287, 818
Hpy99I CGWCG 1 cut(s) 1353
HpyAV CCTTC 4 cut(s) 217, 229, 761, 1144
HpyCH4III ACNGT 3 cut(s) 826, 1178, 1184
HpyCH4IV ACGT 1 cut(s) 1405
HpyF3I CTNAG 9 cut(s) 51, 57, 75, 84, 400, 669, 831, 880, 895
HpySE526I ACGT 1 cut(s) 1405
Hsp92II CATG 6 cut(s) 431, 877, 958, 964, 1296, 1544
HspAI GCGC 1 cut(s) 205
KpnI GGTACC 1 cut(s) 399
Ksp22I TGATCA 2 cut(s) 802, 1390
Kzo9I GATC 3 cut(s) 802, 951, 1390
LguI GCTCTTC 1 cut(s) 1539
LmnI GCTCC 2 cut(s) 904, 1072
LweI GCATC 1 cut(s) 914
MaeI CTAG 3 cut(s) 375, 1056, 1257
MaeII ACGT 1 cut(s) 1405
MaeIII GTNAC 4 cut(s) 826, 1178, 1420, 1441
MalI GATC 3 cut(s) 804, 953, 1392
MboI GATC 3 cut(s) 802, 951, 1390
MboII GAAGA 8 cut(s) 232, 571, 602, 689, 821, 1250, 1401, 1556
MhlI GDGCHC 4 cut(s) 188, 289, 1148, 1164
MlsI TGGCCA 1 cut(s) 959
MluCI AATT 7 cut(s) 115, 664, 748, 761, 999, 1165, 1513
MluNI TGGCCA 1 cut(s) 959
MlyI GAGTC 2 cut(s) 813, 1466
MmeI TCCRAC 1 cut(s) 1372
Mox20I TGGCCA 1 cut(s) 959
MscI TGGCCA 1 cut(s) 959
MseI TTAA 4 cut(s) 180, 486, 1172, 1272
MslI CAYNNNNRTG 1 cut(s) 959
Msp20I TGGCCA 1 cut(s) 959
MspA1I CMGCKG 5 cut(s) 605, 617, 623, 1204, 1337
MspI CCGG 2 cut(s) 389, 1280
MspR9I CCNGG 1 cut(s) 389
Mva1269I GAATGC 2 cut(s) 496, 1495
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 960
NdeII GATC 3 cut(s) 802, 951, 1390
NlaIII CATG 6 cut(s) 431, 877, 958, 964, 1296, 1544
NlaIV GGNNCC 4 cut(s) 397, 915, 1053, 1299
NmuCI GTSAC 3 cut(s) 826, 1178, 1420
NspI RCATGY 1 cut(s) 877
PciSI GCTCTTC 1 cut(s) 1539
PctI GAATGC 2 cut(s) 496, 1495
PfeI GAWTC 2 cut(s) 414, 1376
PflMI CCANNNNNTGG 1 cut(s) 1068
PleI GAGTC 2 cut(s) 813, 1465
PpsI GAGTC 2 cut(s) 813, 1465
PspFI CCCAGC 1 cut(s) 1305
PspN4I GGNNCC 4 cut(s) 397, 915, 1053, 1299
PspPI GGNCC 2 cut(s) 136, 780
PstI CTGCAG 4 cut(s) 601, 622, 628, 1498
PstNI CAGNNNCTG 2 cut(s) 467, 902
PvuII CAGCTG 3 cut(s) 617, 623, 1337
RsaI GTAC 4 cut(s) 397, 522, 869, 966
RsaNI GTAC 4 cut(s) 396, 521, 868, 965
RseI CAYNNNNRTG 1 cut(s) 959
SalI GTCGAC 1 cut(s) 816
SapI GCTCTTC 1 cut(s) 1539
SaqAI TTAA 4 cut(s) 180, 486, 1172, 1272
Sau3AI GATC 3 cut(s) 802, 951, 1390
Sau96I GGNCC 2 cut(s) 136, 780
SchI GAGTC 2 cut(s) 813, 1466
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 4 cut(s) 188, 289, 1148, 1164
SfaNI GCATC 1 cut(s) 914
SfcI CTRYAG 6 cut(s) 199, 441, 597, 618, 624, 1494
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 959
SmlI CTYRAG 3 cut(s) 215, 784, 1096
SmoI CTYRAG 3 cut(s) 215, 784, 1096
Sse9I AATT 7 cut(s) 115, 664, 748, 761, 999, 1165, 1513
SsiI CCGC 3 cut(s) 605, 1013, 1204
SspMI CTAG 3 cut(s) 375, 1056, 1257
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 4 cut(s) 503, 960, 1067, 1193
TaaI ACNGT 3 cut(s) 826, 1178, 1184
TaiI ACGT 1 cut(s) 1408
TaqI TCGA 2 cut(s) 817, 1142
TasI AATT 7 cut(s) 115, 664, 748, 761, 999, 1165, 1513
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 2 cut(s) 414, 1376
Tru1I TTAA 4 cut(s) 180, 486, 1172, 1272
Tru9I TTAA 4 cut(s) 180, 486, 1172, 1272
TscAI CASTG 4 cut(s) 425, 888, 1194, 1371
TseFI GTSAC 3 cut(s) 826, 1178, 1420
Tsp45I GTSAC 3 cut(s) 826, 1178, 1420
TspDTI ATGAA 8 cut(s) 170, 749, 1251, 1257, 1401, 1493, 1506, 1557
TspGWI ACGGA 1 cut(s) 772
TspRI CASTG 4 cut(s) 425, 888, 1194, 1371
Van91I CCANNNNNTGG 1 cut(s) 1068
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 3 cut(s) 115, 664, 1513
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 817
XspI CTAG 3 cut(s) 375, 1056, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.