RLG00000026451

Pre-mRNA-splicing factor cwc25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004249
Physical Location & Seq
Forward (+)
3133252 .. 3133664
413 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026451

Sequence Viewer

Length: 342 bp
ATGACTAGGTACGCTTGCAGGCGCTGGTCGTCGCCATCGTTCAAAATCGGCTCATGTTCGATCTGGTCCAAGACCCGACCTTCCCTCTCGCCGGAGTTCAGTGCTTCTCTCAGTTTGAATACTAGAGGTATTGGTTCCATTGTGACAAATTCCCAATTCAAGCAAGAGCGATTGGAGTTCCTCTATAATTCGGGATTGGCCGTAGGGCCCCCCGGTGGCTCTGATAATTTCAAGGAAGCTTTCACCAAAGCCGAAGAACCGTCTTCTTTCTCCGCCACCGCAAAAAGTATTTATAATCTTTTTAGATTACTGATTGTACGCATTGAGAGGTATAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CWC25 Cir_N

Protein Analysis

114

Amino Acids

12.67

Weight (kDa)

9.94

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AciI CCGC 2 cut(s) 273, 279
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 2 cut(s) 148, 336
AfaI GTAC 2 cut(s) 11, 318
AfiI CCNNNNNNNGG 2 cut(s) 91, 215
AgsI TTSAA 4 cut(s) 43, 118, 160, 232
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 24
AoxI GGCC 2 cut(s) 198, 206
ApaI GGGCCC 1 cut(s) 210
ApoI RAATTY 2 cut(s) 148, 336
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 3 cut(s) 66, 206, 207
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 66
BaeGI GKGCMC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 255
BccI CCATC 1 cut(s) 43
BceAI ACGGC 1 cut(s) 185
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 2 cut(s) 6, 123
BfoI RGCGCY 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 66
BmgT120I GGNCC 3 cut(s) 66, 206, 207
BmiI GGNNCC 3 cut(s) 136, 208, 209
BmrFI CCNGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 215
BseDI CCNNGG 1 cut(s) 211
BseLI CCNNNNNNNGG 2 cut(s) 91, 215
BseMII CTCAG 1 cut(s) 124
BseSI GKGCMC 1 cut(s) 210
BshFI GGCC 2 cut(s) 200, 208
BsiSI CCGG 2 cut(s) 92, 213
BslI CCNNNNNNNGG 2 cut(s) 91, 215
BsnI GGCC 2 cut(s) 200, 208
Bsp120I GGGCCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 60
BspACI CCGC 2 cut(s) 273, 279
BspANI GGCC 2 cut(s) 200, 208
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 136, 208, 209
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 16, 20
BstDEI CTNAG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 1 cut(s) 63
BstMBI GATC 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 255
BsuRI GGCC 2 cut(s) 200, 208
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 16, 20
CaiI CAGNNNCTG 1 cut(s) 24
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 3 cut(s) 66, 206, 207
Csp6I GTAC 2 cut(s) 10, 317
CviAII CATG 1 cut(s) 54
CviJI RGCY 6 cut(s) 51, 200, 208, 219, 239, 251
CviKI_1 RGCY 6 cut(s) 51, 200, 208, 219, 239, 251
CviQI GTAC 2 cut(s) 10, 317
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 198
EciI GGCGGA 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 66
EcoO109I RGGNCCY 2 cut(s) 206, 207
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 1 cut(s) 57
FaiI YATR 4 cut(s) 55, 186, 294, 333
FatI CATG 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 210
FspBI CTAG 2 cut(s) 6, 123
GlaI GCGC 1 cut(s) 23
HaeII RGCGCY 1 cut(s) 25
HaeIII GGCC 2 cut(s) 200, 208
HapII CCGG 2 cut(s) 92, 213
HhaI GCGC 1 cut(s) 24
Hin1II CATG 1 cut(s) 57
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 237
HpaII CCGG 2 cut(s) 92, 213
HphI GGTGA 1 cut(s) 235
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 1 cut(s) 192
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 90
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 1 cut(s) 57
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 60
LpnPI CCDG 5 cut(s) 4, 10, 49, 105, 226
MaeI CTAG 2 cut(s) 6, 123
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 2 cut(s) 255, 266
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 5 cut(s) 148, 155, 187, 226, 336
MnlI CCTC 4 cut(s) 95, 119, 191, 321
MspI CCGG 2 cut(s) 92, 213
MspR9I CCNGG 1 cut(s) 213
NciI CCSGG 1 cut(s) 213
NdeII GATC 1 cut(s) 60
NlaIII CATG 1 cut(s) 57
NlaIV GGNNCC 3 cut(s) 136, 208, 209
NmuCI GTSAC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PsiI TTATAA 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 136, 208, 209
PspOMI GGGCCC 1 cut(s) 206
PspPI GGNCC 3 cut(s) 66, 206, 207
PstNI CAGNNNCTG 1 cut(s) 24
RsaI GTAC 2 cut(s) 11, 318
RsaNI GTAC 2 cut(s) 10, 317
Sau3AI GATC 1 cut(s) 60
Sau96I GGNCC 3 cut(s) 66, 206, 207
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 210
SetI ASST 5 cut(s) 11, 82, 130, 241, 332
SinI GGWCC 1 cut(s) 66
Sse9I AATT 5 cut(s) 148, 155, 187, 226, 336
SsiI CCGC 2 cut(s) 273, 279
SspMI CTAG 2 cut(s) 6, 123
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 59
TasI AATT 5 cut(s) 148, 155, 187, 226, 336
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 2 cut(s) 148, 336
XspI CTAG 2 cut(s) 6, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.