pycom09g19080

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003430
Physical Location & Seq
Reverse (-)
19848495 .. 19850468
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGTTTAATTTAAATGATATAGAAAAAACAAAGGGAATTGTTAATAGGCCAATAACAGTTCCCAAAAAAAAGACTGGGGTTTTCGCCGTTTCAGTTTTCTTCTTCCGAATTGGGGATAAATTGAGTTTTCGCCATTTCGTATCCAGTTCCAGATCTTTACAGTATCGATCGATCCAGAGAGAAGGAATGGGAGTGCTGTCGAACAAGATTGAGAGGGATCTTCTGAAACCTGGAGATCACATCTATTCATGGCGCCACGCGTACCTCTATTCCCACCACGGGATATACGTTGGTGAAGGAAAGGTTATTCACTTCACTCGAGGAGCTGGCCAGGAAACTGGCACCGGAACTGTGTTAGATCGCTTCCTTCTAAGCAGCTCTCCATCCCATTCTTCGGACAATCCCTGCCCAAACTGCGGAGACCAATCGACACTTGATGGTGTCATCTCTTCTTGCATAGATTGTTTTCTATCAGGCGGTCGATCTTTACCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

166

Amino Acids

18.3

Weight (kDa)

9.3

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 75 - 160 3.6e-13 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 252, 341
AccII CGCG 1 cut(s) 260
AciI CCGC 2 cut(s) 417, 477
AclWI GGATC 2 cut(s) 166, 225
AcoI YGGCCR 1 cut(s) 328
AcyI GRCGYC 1 cut(s) 253
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 4 cut(s) 112, 279, 394, 416
AflIII ACRYGT 1 cut(s) 258
AjnI CCWGG 2 cut(s) 229, 330
AluBI AGCT 2 cut(s) 326, 378
AluI AGCT 2 cut(s) 326, 378
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 2 cut(s) 166, 225
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 2 cut(s) 47, 328
ApeKI GCWGC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 318
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 2 cut(s) 252, 341
BbvI GCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 391, 431
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 231, 332
BciVI GTATCC 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 256
BfuI GTATCC 1 cut(s) 151
BglII AGATCT 1 cut(s) 152
BisI GCNGC 1 cut(s) 376
BlsI GCNGC 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 231, 332
BmeT110I CYCGRG 1 cut(s) 318
BmiI GGNNCC 2 cut(s) 254, 343
BmrFI CCNGG 2 cut(s) 231, 332
BmrI ACTGGG 1 cut(s) 84
BmuI ACTGGG 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 252
Bsa29I ATCGAT 2 cut(s) 166, 170
BsaHI GRCGYC 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 277
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 279, 394, 416
Bse1I ACTGG 3 cut(s) 79, 144, 343
BseBI CCWGG 2 cut(s) 231, 332
BseCI ATCGAT 2 cut(s) 166, 170
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 383
BseLI CCNNNNNNNGG 4 cut(s) 112, 279, 394, 416
BseNI ACTGG 3 cut(s) 79, 144, 343
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 2 cut(s) 170, 481
BshFI GGCC 2 cut(s) 49, 330
BshNI GGYRCC 2 cut(s) 252, 341
BshVI ATCGAT 2 cut(s) 166, 170
BsiEI CGRYCG 2 cut(s) 170, 481
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 1 cut(s) 345
BslI CCNNNNNNNGG 4 cut(s) 112, 279, 394, 416
BsmAI GTCTC 1 cut(s) 414
BsnI GGCC 2 cut(s) 49, 330
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 318
Bsp143I GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
BspACI CCGC 2 cut(s) 417, 477
BspANI GGCC 2 cut(s) 49, 330
BspDI ATCGAT 2 cut(s) 166, 170
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 254, 343
BspPI GGATC 2 cut(s) 166, 225
BspT107I GGYRCC 2 cut(s) 252, 341
BspTNI GGTCTC 1 cut(s) 414
BsrI ACTGG 3 cut(s) 79, 144, 343
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
BssNI GRCGYC 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 231, 332
Bst4CI ACNGT 3 cut(s) 58, 162, 352
Bst6I CTCTTC 1 cut(s) 454
BstACI GRCGYC 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 371
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 383
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 7 cut(s) 155, 170, 174, 220, 238, 361, 485
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
BstMCI CGRYCG 2 cut(s) 170, 481
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNI CCWGG 2 cut(s) 231, 332
BstSCI CCNGG 2 cut(s) 229, 330
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 152, 217
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 2 cut(s) 152, 217
Bsu15I ATCGAT 2 cut(s) 166, 170
BsuI GTATCC 1 cut(s) 151
BsuRI GGCC 2 cut(s) 49, 330
BsuTUI ATCGAT 2 cut(s) 166, 170
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 328
CfoI GCGC 1 cut(s) 255
ClaI ATCGAT 2 cut(s) 166, 170
Csp6I GTAC 1 cut(s) 262
CviAII CATG 1 cut(s) 249
CviJI RGCY 4 cut(s) 49, 326, 330, 378
CviKI_1 RGCY 4 cut(s) 49, 326, 330, 378
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 1 cut(s) 371
DinI GGCGCC 1 cut(s) 254
DpnI GATC 7 cut(s) 154, 169, 173, 219, 237, 360, 484
DpnII GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
DraI TTTAAA 1 cut(s) 12
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 229, 330
EgeI GGCGCC 1 cut(s) 254
EheI GGCGCC 1 cut(s) 254
FaeI CATG 1 cut(s) 252
FaiI YATR 4 cut(s) 20, 250, 286, 458
FatI CATG 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 376
FokI GGATG 1 cut(s) 370
Fsp4HI GCNGC 1 cut(s) 376
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 256
HaeIII GGCC 2 cut(s) 49, 330
HapII CCGG 1 cut(s) 345
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 253
Hin1II CATG 1 cut(s) 252
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 305
Hpy188I TCNGA 3 cut(s) 107, 225, 397
Hpy188III TCNNGA 2 cut(s) 150, 175
HpyAV CCTTC 3 cut(s) 176, 290, 377
HpyCH4III ACNGT 3 cut(s) 58, 162, 352
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 1 cut(s) 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 1 cut(s) 253
Hsp92II CATG 1 cut(s) 252
HspAI GCGC 1 cut(s) 253
KasI GGCGCC 1 cut(s) 252
Kzo9I GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 1 cut(s) 387
MaeII ACGT 1 cut(s) 288
MalI GATC 7 cut(s) 154, 169, 173, 219, 237, 360, 484
MboI GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
MboII GAAGA 5 cut(s) 91, 94, 212, 384, 441
MflI RGATCY 2 cut(s) 152, 217
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 4 cut(s) 7, 36, 108, 119
MluI ACGCGT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 330
Mly113I GGCGCC 1 cut(s) 253
MnlI CCTC 3 cut(s) 207, 275, 314
Mox20I TGGCCA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 330
MseI TTAA 3 cut(s) 6, 11, 42
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 231, 332
MvaI CCWGG 2 cut(s) 231, 332
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 414
NarI GGCGCC 1 cut(s) 253
NdeII GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
NlaIII CATG 1 cut(s) 252
NlaIV GGNNCC 2 cut(s) 254, 343
PaeR7I CTCGAG 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PkrI GCNGC 1 cut(s) 377
Ple19I CGATCG 1 cut(s) 170
PluTI GGCGCC 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 229, 330
PspGI CCWGG 2 cut(s) 229, 330
PspN4I GGNNCC 2 cut(s) 254, 343
PspXI VCTCGAGB 1 cut(s) 318
PsuI RGATCY 2 cut(s) 152, 217
PvuI CGATCG 1 cut(s) 170
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 6, 11, 42
SatI GCNGC 1 cut(s) 376
Sau3AI GATC 7 cut(s) 152, 167, 171, 217, 235, 358, 482
ScrFI CCNGG 2 cut(s) 231, 332
SetI ASST 7 cut(s) 232, 267, 291, 306, 328, 380, 493
SfoI GGCGCC 1 cut(s) 254
Sfr274I CTCGAG 1 cut(s) 318
SlaI CTCGAG 1 cut(s) 318
SmiI ATTTAAAT 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
Sse9I AATT 4 cut(s) 7, 36, 108, 119
SsiI CCGC 2 cut(s) 417, 477
SspDI GGCGCC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 229, 330
SwaI ATTTAAAT 1 cut(s) 12
TaaI ACNGT 3 cut(s) 58, 162, 352
TaiI ACGT 1 cut(s) 291
TaqI TCGA 6 cut(s) 166, 170, 200, 319, 428, 481
TasI AATT 4 cut(s) 7, 36, 108, 119
Tru1I TTAA 3 cut(s) 6, 11, 42
Tru9I TTAA 3 cut(s) 6, 11, 42
TseI GCWGC 1 cut(s) 375
TspDTI ATGAA 1 cut(s) 237
XhoI CTCGAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.