Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

905799 .. 911981

Gene Structure 6183 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGGTGAAATGTGCGATCGGTCGTGAGCGGGAGGTAGCTGTTTGCTTAATGCAAAAGTATATCGATAAACCTGAACTGCAGATCAGATCTGTTATTGCTCTTGACCACCTTAAAAACTATGTATACGTTGAAGCGGACAAAGAAGCCCATGTGAGGGAGGCTTGCAAAGGTCTGCGCAATATATTTGGCCAGAAAATAAAGCTTGTTCCAATTAGAGAAATGACTGATGTTCTTTCGGTTGAAAGCAAGGCTGTTGATCTTTCTAGGGATACGTGGGTCAGAATGAAGAGTGGGACATATAAAGGAGACCTTGCCCAGGTTGCGGATGTGGACAATGTGCGGCAGAGAGTTACAGTTAAATTAATTCCAAGGATTGATTTACAAGCTCTTGCAAATAAAATGGATGGCAGAGAAGTTGTGAGAACAAAAGCATTTGTTCCTCCTCCACGTTTATTAAATATTGAGGAAGCAAGGGAACTGCATATTCTTGGAGATCGTAAACAAGATCGGATGACTGGTGATTATTATCAGACGATTAATGGCATGATGTTTAAAGATGGTTTCCTGTATAAAGCAGTATCAATCAAATCAATTAGTACTCAGAACATACATCCCACGTTTGACGAACTTGAAAAATTTCGGAAGCCCAGCGAGAATGGTGATGGAGATGTTGCAAGTCTGTCAACTTTATTTTCAAACAGAAAGAAGGGACACTTTCTGAAGGGCGATGCAGTTATTGTTGTCAAGGGAGATCTAAAAAATTTGAAAGGATGGGTTGAGAAAGTTGAGGAGGAGACTGTTCATATCAGACCCGAAATGAAAGAACTCCCAAAAACACTTGCCATCAATGAAAAAGAGCTTTGCAAATACTTCGAACCTGGGAATCATGTGAAAGTTGTATCTGGCACTCAGGAAGGTGCAACTGGTATGGTTGTTAAGGTTGAGCAGCATGTTCTCATCATCTTGTCTGATGTAACGAAAGAACATATCGATCTGTTTGCTGATGATGTTGTGGAGAGCTCTGAGGTGACATCTGGTATTACCAGAATTGGGGCATATGAGCACTTCATCGATCTCGTGCTACTGGATAATAATAGCTTCGGTGTAATCATACGCGTAGAAAGTGAAGCATTCCAGGTTCTTAAGGGAGTTCCTGATAGACCTGAGGTTGCCCTTGTCAAGTTAAGAGAGGTCAAATGCAAGATTGAGAAGAGTTTTCCTGTGGAAGTTAAGTATAAACACAAGGTATCTGTGAAGGATGTTGTGAGGGTCATTGATGGCCCTTGTGAGGGGAAACAGGGTCCTGTGCAACACATATACAGAGGAGTCTTGTTCATCTTTGATCGCCATCACCTGGAGCATGCAGGCTTTATCTGTGTTAAGTCTCATAATTGTGCTCTTGTGGGAGGTTCACGTGCTAATGGCGATAGAAATGGTAACTCCAATTCACGATATGACCACCTCAGAACTCCTCCTCGTATTCCTCAGTCCCCGAATAGGTTTTCTAGAGGAGGACCTCCATCTAACTATGGAGGAAGGAATAGAGGTGGACGAGGGCATGATGGCTTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGTTATCGTGGACGTGTTGTTGAGGTTAAGGGACCAAATGTACGGGTTGAGTTGGAGTCCCAAATGAAGGTTGTTACAGTTGATCGTAATTGTATTTCTGATAATGTGGCTGTTACCACACCTTATCGTGATACATCTCGCTACGGTATGGGAAGTGAGACTCCCATGCATCCTTCTCGAACTCCTCTCCATCCATATATGACTCCTATGAGGGATGCTGCAGCAACACCTATACATGATGGCATGAGGACTCCTATGCGTGACCGAGCATGGAATCCATATGCACCTATGAGTCCAGCTAGGGATAACTGGGAGGAAGGAAACCCTGCTTCTTGGGGTGCTAGCCCACAGTATCAGCCAGGAAGTCCTCCTTCGCGGGCGTATGAAGCACCAACTCCTGGTTCAGGTTGGGCAAACACTCCTGGTGGAAATTATAGTGAAGCTGGTACACCGAGGGACAGTAGTTCCGCTTATGCAAATGCTCCGAGCCCTTACTTGCCATCAACTCCAGGTGGCCAGCCTATGACACCAAATTCGGCATCCTACCTTCCTGGAACCCCTGGAGGGCAGCCAATGACACCAGGCACTGGTGGTCTGGATATGTTGTCTCCTGTTATAGGTGGGGACAGTGAAGGACCGTGGTTCCTCCCAGACATATTGGTCAGTGTACACAGTTCTGGAGAGGAAACTACTGGTGCTGTGAGAGAAGTGCTTCCGGATGGCTCCTGTAGGGTAGCTATTGGGTCGGGTGGCAACGGGGAAACTATAATAGCACTTCCAAATGAAATGGAGGCGGTGGTACCGAAGAAAAGTGATAAGATAAAGATCATCGGCGGGTCACTACGAGGTGCGACCGGTAAGCTGATTGGTGTTGATGGCACAGACGGAATTGTGAAGGTAGATGACACTCTTGATGTTAAGATTTTGGACTTGGCTATTTTGTCAAAACTAGCCCAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

857

Amino Acids

93.81

Weight (kDa)

8.61

Isoelectric Point (pI)

31.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	2 - 78	5e-25	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	84 - 213	7.6e-68	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	241 - 290	9.5e-23	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	356 - 401	2.9e-12	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	412 - 462	4.1e-21	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	523 - 566	1.3e-17	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	751 - 800	4.4e-24	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	802 - 853	4.9e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2267
Acc16I	TGCGCA	1 cut(s)	176
Acc65I	GGTACC	1 cut(s)	2407
AccB1I	GGYRCC	2 cut(s)	1598, 2407
AccB7I	CCANNNNNTGG	3 cut(s)	1354, 2006, 2195
AccBSI	CCGCTC	1 cut(s)	28
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	2 cut(s)	1116, 1984
AccIII	TCCGGA	1 cut(s)	2323
AciI	CCGC	8 cut(s)	28, 134, 323, 340, 1984, 2076, 2402, 2442
AcoI	YGGCCR	2 cut(s)	187, 2122
AcsI	RAATTY	3 cut(s)	635, 760, 2140
AcuI	CTGAAG	1 cut(s)	740
AcvI	CACGTG	1 cut(s)	1415
AfaI	GTAC	5 cut(s)	598, 1650, 2056, 2277, 2409
AflII	CTTAAG	1 cut(s)	1142
AflIII	ACRYGT	2 cut(s)	1114, 1621
AgeI	ACCGGT	1 cut(s)	2462
AgsI	TTSAA	5 cut(s)	131, 242, 632, 696, 766
AjiI	CACGTC	1 cut(s)	1622
AjuI	GAANNNNNNNTTGG	2 cut(s)	1993, 2025
Alw21I	GWGCWC	3 cut(s)	1022, 1065, 1399
Alw26I	GTCTC	5 cut(s)	300, 788, 1389, 1760, 2220
AlwNI	CAGNNNCTG	1 cut(s)	2195
Aor13HI	TCCGGA	1 cut(s)	2323
AoxI	GGCC	3 cut(s)	187, 1279, 2122
ApeKI	GCWGC	4 cut(s)	946, 1826, 1829, 2176
ApoI	RAATTY	3 cut(s)	635, 760, 2140
ArsI	GACNNNNNNTTYG	2 cut(s)	2125, 2157
AseI	ATTAAT	2 cut(s)	362, 537
AsiGI	ACCGGT	1 cut(s)	2462
Asp700I	GAANNNNTTC	2 cut(s)	636, 2319
Asp718I	GGTACC	1 cut(s)	2407
AspLEI	GCGC	1 cut(s)	177
AspS9I	GGNCC	5 cut(s)	1280, 1301, 1514, 1640, 2243
AsuHPI	GGTGA	5 cut(s)	16, 530, 671, 1039, 1343
AsuII	TTCGAA	1 cut(s)	873
AsuNHI	GCTAGC	1 cut(s)	1949
AvaII	GGWCC	4 cut(s)	1301, 1514, 1640, 2243
AxyI	CCTNAGG	1 cut(s)	1164
BaeI	ACNNNNGTAYC	2 cut(s)	261, 294
BalI	TGGCCA	2 cut(s)	189, 2124
BanI	GGYRCC	2 cut(s)	1598, 2407
BanII	GRGCYC	2 cut(s)	1022, 2099
BauI	CACGAG	1 cut(s)	1076
BbrPI	CACGTG	1 cut(s)	1415
Bbv12I	GWGCWC	3 cut(s)	1022, 1065, 1399
BbvI	GCAGC	4 cut(s)	958, 1813, 1841, 2188
BcgI	CGANNNNNNTGC	6 cut(s)	853, 887, 980, 1014, 1766, 1800
BciVI	GTATCC	1 cut(s)	262
BcoDI	GTCTC	5 cut(s)	300, 788, 1389, 1760, 2220
BfaI	CTAG	5 cut(s)	264, 1506, 1908, 1950, 2558
BfmI	CTRYAG	4 cut(s)	77, 1578, 1827, 2335
BfrI	CTTAAG	1 cut(s)	1142
BfuI	GTATCC	1 cut(s)	262
BglII	AGATCT	2 cut(s)	86, 751
BisI	GCNGC	5 cut(s)	341, 947, 1827, 1830, 2177
BlsI	GCNGC	5 cut(s)	342, 948, 1828, 1831, 2178
BmcAI	AGTACT	1 cut(s)	598
Bme18I	GGWCC	4 cut(s)	1301, 1514, 1640, 2243
BmgBI	CACGTC	1 cut(s)	1622
BmgT120I	GGNCC	5 cut(s)	1280, 1301, 1514, 1640, 2243
BmiI	GGNNCC	7 cut(s)	1302, 1600, 1641, 2164, 2252, 2332, 2409
BmrI	ACTGGG	1 cut(s)	1927
BmsI	GCATC	4 cut(s)	718, 1786, 1813, 2156
BmtI	GCTAGC	1 cut(s)	1953
BmuI	ACTGGG	1 cut(s)	1927
BpmI	CTGGAG	4 cut(s)	1376, 2100, 2190, 2307
Bpu14I	TTCGAA	1 cut(s)	873
Bsa29I	ATCGAT	3 cut(s)	63, 990, 1071
BsaAI	YACGTR	2 cut(s)	273, 1415
BsaBI	GATNNNNATC	3 cut(s)	525, 1347, 2432
BsaI	GGTCTC	1 cut(s)	300
BsaJI	CCNNGG	7 cut(s)	315, 368, 878, 1594, 2060, 2167, 2246
BsaWI	WCCGGW	2 cut(s)	2323, 2462
Bse118I	RCCGGY	1 cut(s)	2462
Bse1I	ACTGG	6 cut(s)	520, 928, 1089, 1922, 2200, 2305
Bse21I	CCTNAGG	1 cut(s)	1164
Bse8I	GATNNNNATC	3 cut(s)	525, 1347, 2432
BseAI	TCCGGA	1 cut(s)	2323
BseCI	ATCGAT	3 cut(s)	63, 990, 1071
BseDI	CCNNGG	7 cut(s)	315, 368, 878, 1594, 2060, 2167, 2246
BseJI	GATNNNNATC	3 cut(s)	525, 1347, 2432
BseMII	CTCAG	6 cut(s)	614, 923, 1014, 1155, 1477, 1499
BseNI	ACTGG	6 cut(s)	520, 928, 1089, 1922, 2200, 2305
BseRI	GAGGAG	8 cut(s)	432, 803, 806, 1338, 1461, 1464, 1524, 1782
BseXI	GCAGC	4 cut(s)	958, 1813, 1841, 2188
BseYI	CCCAGC	1 cut(s)	647
Bsh1236I	CGCG	2 cut(s)	1116, 1984
Bsh1285I	CGRYCG	3 cut(s)	18, 22, 2463
BshFI	GGCC	3 cut(s)	189, 1281, 2124
BshNI	GGYRCC	2 cut(s)	1598, 2407
BshTI	ACCGGT	1 cut(s)	2462
BshVI	ATCGAT	3 cut(s)	63, 990, 1071
BsiEI	CGRYCG	3 cut(s)	18, 22, 2463
BsiHKAI	GWGCWC	3 cut(s)	1022, 1065, 1399
BsiSI	CCGG	2 cut(s)	2324, 2463
BslFI	GGGAC	8 cut(s)	307, 723, 1474, 1588, 1651, 1653, 2078, 2246
BsmAI	GTCTC	5 cut(s)	300, 788, 1389, 1760, 2220
BsmFI	GGGAC	8 cut(s)	307, 723, 1474, 1588, 1651, 1653, 2078, 2246
BsmI	GAATGC	1 cut(s)	1130
BsnI	GGCC	3 cut(s)	189, 1281, 2124
Bso31I	GGTCTC	1 cut(s)	300
Bsp119I	TTCGAA	1 cut(s)	873
Bsp1286I	GDGCHC	4 cut(s)	1022, 1065, 1399, 2099
Bsp13I	TCCGGA	1 cut(s)	2323
Bsp1407I	TGTACA	1 cut(s)	2275
BspACI	CCGC	8 cut(s)	28, 134, 323, 340, 1984, 2076, 2402, 2442
BspANI	GGCC	3 cut(s)	189, 1281, 2124
BspCNI	CTCAG	6 cut(s)	613, 922, 1015, 1156, 1476, 1498
BspDI	ATCGAT	3 cut(s)	63, 990, 1071
BspEI	TCCGGA	1 cut(s)	2323
BspFNI	CGCG	2 cut(s)	1116, 1984
BspLI	GGNNCC	7 cut(s)	1302, 1600, 1641, 2164, 2252, 2332, 2409
BspMAI	CTGCAG	2 cut(s)	81, 1831
BspOI	GCTAGC	1 cut(s)	1953
BspT104I	TTCGAA	1 cut(s)	873
BspT107I	GGYRCC	2 cut(s)	1598, 2407
BspTI	CTTAAG	1 cut(s)	1142
BspTNI	GGTCTC	1 cut(s)	300
BsrBI	CCGCTC	1 cut(s)	28
BsrFI	RCCGGY	1 cut(s)	2462
BsrGI	TGTACA	1 cut(s)	2275
BsrI	ACTGG	6 cut(s)	520, 928, 1089, 1922, 2200, 2305
BssAI	RCCGGY	1 cut(s)	2462
BssECI	CCNNGG	7 cut(s)	315, 368, 878, 1594, 2060, 2167, 2246
BssNAI	GTATAC	1 cut(s)	124
BssSI	CACGAG	1 cut(s)	1076
BssT1I	CCWWGG	1 cut(s)	368
Bst1107I	GTATAC	1 cut(s)	124
Bst2BI	CACGAG	1 cut(s)	1076
Bst6I	CTCTTC	2 cut(s)	281, 1205
BstAFI	CTTAAG	1 cut(s)	1142
BstAUI	TGTACA	1 cut(s)	2275
BstBAI	YACGTR	2 cut(s)	273, 1415
BstBI	TTCGAA	1 cut(s)	873
BstC8I	GCNNGC	6 cut(s)	163, 1362, 1366, 1951, 1986, 2126
BstDEI	CTNAG	6 cut(s)	600, 909, 1023, 1164, 1463, 1485
BstDSI	CCRYGG	1 cut(s)	2246
BstENI	CCTNNNNNAGG	3 cut(s)	314, 2010, 2223
BstFNI	CGCG	2 cut(s)	1116, 1984
BstHHI	GCGC	1 cut(s)	177
BstMAI	GTCTC	5 cut(s)	300, 788, 1389, 1760, 2220
BstMCI	CGRYCG	3 cut(s)	18, 22, 2463
BstMWI	GCNNNNNNNGC	2 cut(s)	320, 1994
BstNSI	RCATGY	2 cut(s)	953, 1364
BstSFI	CTRYAG	4 cut(s)	77, 1578, 1827, 2335
BstUI	CGCG	2 cut(s)	1116, 1984
BstV1I	GCAGC	4 cut(s)	958, 1813, 1841, 2188
BstX2I	RGATCY	2 cut(s)	86, 751
BstYI	RGATCY	2 cut(s)	86, 751
BstZ17I	GTATAC	1 cut(s)	124
Bsu15I	ATCGAT	3 cut(s)	63, 990, 1071
Bsu36I	CCTNAGG	1 cut(s)	1164
BsuI	GTATCC	1 cut(s)	262
BsuRI	GGCC	3 cut(s)	189, 1281, 2124
BsuTUI	ATCGAT	3 cut(s)	63, 990, 1071
BtgI	CCRYGG	1 cut(s)	2246
BtgZI	GCGATG	1 cut(s)	741
BtrI	CACGTC	1 cut(s)	1622
BtsIMutI	CAGTG	3 cut(s)	2193, 2242, 2278
Cac8I	GCNNGC	6 cut(s)	163, 1362, 1366, 1951, 1986, 2126
CaiI	CAGNNNCTG	1 cut(s)	2195
CfoI	GCGC	1 cut(s)	177
Cfr10I	RCCGGY	1 cut(s)	2462
Cfr13I	GGNCC	5 cut(s)	1280, 1301, 1514, 1640, 2243
ClaI	ATCGAT	3 cut(s)	63, 990, 1071
Csp6I	GTAC	5 cut(s)	597, 1649, 2055, 2276, 2408
CspAI	ACCGGT	1 cut(s)	2462
CviQI	GTAC	5 cut(s)	597, 1649, 2055, 2276, 2408
DdeI	CTNAG	6 cut(s)	600, 909, 1023, 1164, 1463, 1485
DraI	TTTAAA	1 cut(s)	553
DrdI	GACNNNNNNGTC	1 cut(s)	2267
DseDI	GACNNNNNNGTC	1 cut(s)	2267
EaeI	YGGCCR	2 cut(s)	187, 2122
Eam1104I	CTCTTC	2 cut(s)	281, 1205
EarI	CTCTTC	2 cut(s)	281, 1205
Ecl136II	GAGCTC	1 cut(s)	1020
Eco130I	CCWWGG	1 cut(s)	368
Eco24I	GRGCYC	2 cut(s)	1022, 2099
Eco31I	GGTCTC	1 cut(s)	300
Eco47I	GGWCC	4 cut(s)	1301, 1514, 1640, 2243
Eco53kI	GAGCTC	1 cut(s)	1020
Eco57I	CTGAAG	1 cut(s)	740
Eco72I	CACGTG	1 cut(s)	1415
Eco81I	CCTNAGG	1 cut(s)	1164
EcoICRI	GAGCTC	1 cut(s)	1020
EcoNI	CCTNNNNNAGG	3 cut(s)	314, 2010, 2223
EcoO109I	RGGNCCY	2 cut(s)	1301, 1514
EcoT14I	CCWWGG	1 cut(s)	368
EcoT22I	ATGCAT	1 cut(s)	1779
EcoT38I	GRGCYC	2 cut(s)	1022, 2099
ErhI	CCWWGG	1 cut(s)	368
FalI	AAGNNNNNCTT	6 cut(s)	294, 326, 698, 730, 1963, 1995
FaqI	GGGAC	8 cut(s)	307, 723, 1474, 1588, 1651, 1653, 2078, 2246
FauI	CCCGC	3 cut(s)	21, 1977, 2435
FauNDI	CATATG	2 cut(s)	1057, 1888
FblI	GTMKAC	1 cut(s)	123
Fnu4HI	GCNGC	5 cut(s)	341, 947, 1827, 1830, 2177
FriOI	GRGCYC	2 cut(s)	1022, 2099
Fsp4HI	GCNGC	5 cut(s)	341, 947, 1827, 1830, 2177
FspBI	CTAG	5 cut(s)	264, 1506, 1908, 1950, 2558
FspI	TGCGCA	1 cut(s)	176
GlaI	GCGC	1 cut(s)	176
GluI	GCNGC	5 cut(s)	341, 947, 1827, 1830, 2177
GsaI	CCCAGC	1 cut(s)	651
GsuI	CTGGAG	4 cut(s)	1376, 2100, 2190, 2307
HaeIII	GGCC	3 cut(s)	189, 1281, 2124
HapII	CCGG	2 cut(s)	2324, 2463
HhaI	GCGC	1 cut(s)	177
Hin6I	GCGC	1 cut(s)	175
HinP1I	GCGC	1 cut(s)	175
HincII	GTYRAC	1 cut(s)	684
HindII	GTYRAC	1 cut(s)	684
HindIII	AAGCTT	1 cut(s)	200
HinfI	GANTC	9 cut(s)	883, 1326, 1588, 1664, 1768, 1810, 1858, 1882, 1900
HpaII	CCGG	2 cut(s)	2324, 2463
HphI	GGTGA	5 cut(s)	16, 530, 671, 1039, 1343
HpyCH4IV	ACGT	6 cut(s)	126, 272, 448, 617, 1414, 1621
HpyF10VI	GCNNNNNNNGC	2 cut(s)	320, 1994
HpyF3I	CTNAG	6 cut(s)	600, 909, 1023, 1164, 1463, 1485
HpySE526I	ACGT	6 cut(s)	126, 272, 448, 617, 1414, 1621
HspAI	GCGC	1 cut(s)	175
Kpn2I	TCCGGA	1 cut(s)	2323
KpnI	GGTACC	1 cut(s)	2411
LmnI	GCTCC	3 cut(s)	1357, 2095, 2336
Lsp1109I	GCAGC	4 cut(s)	958, 1813, 1841, 2188
LweI	GCATC	4 cut(s)	718, 1786, 1813, 2156
MaeI	CTAG	5 cut(s)	264, 1506, 1908, 1950, 2558
MaeII	ACGT	6 cut(s)	126, 272, 448, 617, 1414, 1621
MaeIII	GTNAC	8 cut(s)	349, 973, 1027, 1436, 1681, 1720, 1868, 2445
MbiI	CCGCTC	1 cut(s)	28
MboII	GAAGA	3 cut(s)	298, 1222, 2425
MflI	RGATCY	2 cut(s)	86, 751
MhlI	GDGCHC	4 cut(s)	1022, 1065, 1399, 2099
MlsI	TGGCCA	2 cut(s)	189, 2124
MluI	ACGCGT	1 cut(s)	1114
MluNI	TGGCCA	2 cut(s)	189, 2124
MlyI	GAGTC	6 cut(s)	1335, 1673, 1762, 1804, 1852, 1909
MmeI	TCCRAC	1 cut(s)	1641
Mox20I	TGGCCA	2 cut(s)	189, 2124
Mph1103I	ATGCAT	1 cut(s)	1779
MroI	TCCGGA	1 cut(s)	2323
MroXI	GAANNNNTTC	2 cut(s)	636, 2319
MscI	TGGCCA	2 cut(s)	189, 2124
MslI	CAYNNNNRTG	1 cut(s)	1392
Msp20I	TGGCCA	2 cut(s)	189, 2124
MspCI	CTTAAG	1 cut(s)	1142
MspI	CCGG	2 cut(s)	2324, 2463
Mva1269I	GAATGC	1 cut(s)	1130
MvnI	CGCG	2 cut(s)	1116, 1984
MwoI	GCNNNNNNNGC	2 cut(s)	320, 1994
NdeI	CATATG	2 cut(s)	1057, 1888
NheI	GCTAGC	1 cut(s)	1949
NlaIV	GGNNCC	7 cut(s)	1302, 1600, 1641, 2164, 2252, 2332, 2409
NmuCI	GTSAC	3 cut(s)	1027, 1868, 2445
NsbI	TGCGCA	1 cut(s)	176
NsiI	ATGCAT	1 cut(s)	1779
NspI	RCATGY	2 cut(s)	953, 1364
NspV	TTCGAA	1 cut(s)	873
PaeI	GCATGC	1 cut(s)	1364
PctI	GAATGC	1 cut(s)	1130
PdmI	GAANNNNTTC	2 cut(s)	636, 2319
PfeI	GAWTC	3 cut(s)	883, 1588, 1882
PflMI	CCANNNNNTGG	3 cut(s)	1354, 2006, 2195
PfoI	TCCNGGA	1 cut(s)	2158
PinAI	ACCGGT	1 cut(s)	2462
PkrI	GCNGC	5 cut(s)	342, 948, 1828, 1831, 2178
Ple19I	CGATCG	1 cut(s)	18
PleI	GAGTC	6 cut(s)	1334, 1672, 1762, 1804, 1852, 1908
PmaCI	CACGTG	1 cut(s)	1415
PmlI	CACGTG	1 cut(s)	1415
PpsI	GAGTC	6 cut(s)	1334, 1672, 1762, 1804, 1852, 1908
Ppu21I	YACGTR	2 cut(s)	273, 1415
PpuMI	RGGWCCY	2 cut(s)	1301, 1514
PshBI	ATTAAT	2 cut(s)	362, 537
Psp124BI	GAGCTC	1 cut(s)	1022
Psp5II	RGGWCCY	2 cut(s)	1301, 1514
PspCI	CACGTG	1 cut(s)	1415
PspFI	CCCAGC	1 cut(s)	647
PspN4I	GGNNCC	7 cut(s)	1302, 1600, 1641, 2164, 2252, 2332, 2409
PspPI	GGNCC	5 cut(s)	1280, 1301, 1514, 1640, 2243
PspPPI	RGGWCCY	2 cut(s)	1301, 1514
PstI	CTGCAG	2 cut(s)	81, 1831
PstNI	CAGNNNCTG	1 cut(s)	2195
PsuI	RGATCY	2 cut(s)	86, 751
PvuI	CGATCG	1 cut(s)	18
RsaI	GTAC	5 cut(s)	598, 1650, 2056, 2277, 2409
RsaNI	GTAC	5 cut(s)	597, 1649, 2055, 2276, 2408
RseI	CAYNNNNRTG	1 cut(s)	1392
SacI	GAGCTC	1 cut(s)	1022
SatI	GCNGC	5 cut(s)	341, 947, 1827, 1830, 2177
Sau96I	GGNCC	5 cut(s)	1280, 1301, 1514, 1640, 2243
ScaI	AGTACT	1 cut(s)	598
SchI	GAGTC	6 cut(s)	1335, 1673, 1762, 1804, 1852, 1909
SduI	GDGCHC	4 cut(s)	1022, 1065, 1399, 2099
SfaNI	GCATC	4 cut(s)	718, 1786, 1813, 2156
SfcI	CTRYAG	4 cut(s)	77, 1578, 1827, 2335
SfuI	TTCGAA	1 cut(s)	873
SinI	GGWCC	4 cut(s)	1301, 1514, 1640, 2243
SmiMI	CAYNNNNRTG	1 cut(s)	1392
SmlI	CTYRAG	1 cut(s)	1142
SmoI	CTYRAG	1 cut(s)	1142
SphI	GCATGC	1 cut(s)	1364
SsiI	CCGC	8 cut(s)	28, 134, 323, 340, 1984, 2076, 2402, 2442
SspI	AATATT	1 cut(s)	460
SspMI	CTAG	5 cut(s)	264, 1506, 1908, 1950, 2558
SstI	GAGCTC	1 cut(s)	1022
StyI	CCWWGG	1 cut(s)	368
TaiI	ACGT	6 cut(s)	129, 275, 451, 620, 1417, 1624
TaqI	TCGA	5 cut(s)	63, 873, 990, 1071, 1786
TaqII	GACCGA	2 cut(s)	8, 1887
TatI	WGTACW	2 cut(s)	596, 2275
TauI	GCSGC	1 cut(s)	343
TfiI	GAWTC	3 cut(s)	883, 1588, 1882
TscAI	CASTG	3 cut(s)	2200, 2242, 2278
TseFI	GTSAC	3 cut(s)	1027, 1868, 2445
TseI	GCWGC	4 cut(s)	946, 1826, 1829, 2176
Tsp45I	GTSAC	3 cut(s)	1027, 1868, 2445
TspDTI	ATGAA	9 cut(s)	299, 791, 833, 864, 1057, 1324, 1688, 2007, 2406
TspGWI	ACGGA	1 cut(s)	2508
TspRI	CASTG	3 cut(s)	2200, 2242, 2278
Van91I	CCANNNNNTGG	3 cut(s)	1354, 2006, 2195
Vha464I	CTTAAG	1 cut(s)	1142
VpaK11BI	GGWCC	4 cut(s)	1301, 1514, 1640, 2243
VspI	ATTAAT	2 cut(s)	362, 537
XagI	CCTNNNNNAGG	3 cut(s)	314, 2010, 2223
XapI	RAATTY	3 cut(s)	635, 760, 2140
XbaI	TCTAGA	1 cut(s)	1505
XceI	RCATGY	2 cut(s)	953, 1364
XmiI	GTMKAC	1 cut(s)	123
XmnI	GAANNNNTTC	2 cut(s)	636, 2319
XspI	CTAG	5 cut(s)	264, 1506, 1908, 1950, 2558
ZrmI	AGTACT	1 cut(s)	598
Zsp2I	ATGCAT	1 cut(s)	1779

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.