pycom10g22160

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002718
Physical Location & Seq
Reverse (-)
24609979 .. 24611480
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
ATGGGAGAAGCTCAAGAACTTCAACTCAGCATCGGGGTATCTGTTCTTATAGTTAAGGAAGGAAATGGAGAAAGTGAAGCAAACTTATCAGGGCCTACACATGTCTCCGACCGATCATTGCTACCGCAGCGCAAAAGTGTCAACTGGTGGATTAGAATCGCCATTTACTCGTTCCTTGTCATTGCCGGCCAGTCAATTGCAACATTCTTGGGAAGACAGTACTACGACAAGGGCGCCAAAAGTAATTGGTTGGCAACGGTAGTACAAATTTGTGGCTTTCCAATCATGCTTCCCTACTATTGCATCCCAGCAAGCAGAAATAATCCAATCCCAAAAGATAATCCTATCCCATCAAAATCACCGTCTACCAAAATTCTCGCATCAGTATATGTCTCTCTTGGCATCCTAATAGCCCTAGACTGCTACTTGTATTCCGTAGGACTATCTTACCTTCCTGTATCTACTTTTACCCTCATTTGTGCATCTCAATTGGCCTTCAACGCGTTTTTCTCCTTCTTCCTCAACGCGCAAAAGTTCACTTCTTACATTGTCAATTCTCTAGTTCTCCTCACCATCTCCTCCACCCTCCTTGTGTTCCAAGGTGAAGACGACTCTGGAGACAATGATCCAAGTGGACGATCGAAGGCAAAGTATGCAATTGGGTTCATATGCACCTTAGGGGCATCAGCCGGAGCCGGATTATTGCTTTCCCTAACTCAGCTTGCCTTCAGAAGGGTTATAAAACAGGGGACGTTTAGAGCGGTCATGGACCTGATAGTGTGTGAAAATACGGTCGCAACCTGTGTAACCGTGGTCGGACTTTTCGCTAGCGGAGAGTGGAAGCATTTGAAGGGTGAGATGGAGGGGTATAAATTAGGGAAGATATCCTATGTGATGAATGTGACATGGACAGCAATAACATGGCAGCTTTTTTCAGTTGGAACGGTAGGCTTGATTGTGGAAGCCTCTTCCCTCTTCTCCAATTCCATAAGTGCTTTGGGTTTGCCTGCTGTTCCGGTTCTAGCTGTGATCTTCTTCCACGAAAAAATGAGTGGAATTAAGGGAATAGCCATGGCTTTGGGTATTTGGGGATTTGTTTCCTACGCGTATCATCACTACATCGACGATCGTAAGTCCAAGGCGGAGAAAAGAAATTCCAGGAACGTTGAAGTTCCGAAAGCTTTAAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

398

Amino Acids

43.23

Weight (kDa)

8.92

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 51 - 378 1.4e-120 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
AccB1I GGYRCC 1 cut(s) 233
AccBSI CCGCTC 1 cut(s) 761
AccI GTMKAC 1 cut(s) 365
AccII CGCG 3 cut(s) 503, 527, 1106
AciI CCGC 4 cut(s) 125, 761, 831, 1142
AclI AACGTT 1 cut(s) 1164
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 3 cut(s) 267, 372, 1153
AcuI CTGAAG 1 cut(s) 712
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 221, 264
AfiI CCNNNNNNNGG 1 cut(s) 732
AflIII ACRYGT 3 cut(s) 100, 501, 1104
AgsI TTSAA 4 cut(s) 23, 499, 850, 1169
AjnI CCWGG 1 cut(s) 1157
AluBI AGCT 5 cut(s) 11, 721, 928, 1025, 1181
AluI AGCT 5 cut(s) 11, 721, 928, 1025, 1181
Alw26I GTCTC 3 cut(s) 109, 397, 612
AlwI GGATC 1 cut(s) 620
AoxI GGCC 3 cut(s) 92, 187, 492
ApeKI GCWGC 2 cut(s) 127, 925
ApoI RAATTY 3 cut(s) 267, 372, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 3 cut(s) 132, 236, 529
AspS9I GGNCC 2 cut(s) 92, 769
AsuHPI GGTGA 4 cut(s) 351, 562, 614, 866
AsuNHI GCTAGC 1 cut(s) 827
AvaII GGWCC 1 cut(s) 769
AxyI CCTNAGG 1 cut(s) 676
BanI GGYRCC 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 220, 612
BbvI GCAGC 2 cut(s) 139, 937
BccI CCATC 3 cut(s) 358, 581, 853
BciT130I CCWGG 1 cut(s) 1159
BcoDI GTCTC 3 cut(s) 109, 397, 612
BfaI CTAG 4 cut(s) 416, 560, 828, 1022
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 2 cut(s) 128, 926
BlsI GCNGC 2 cut(s) 129, 927
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 1 cut(s) 1159
Bme18I GGWCC 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 92, 769
BmiI GGNNCC 2 cut(s) 235, 694
BmrFI CCNGG 1 cut(s) 1159
BmsI GCATC 6 cut(s) 39, 312, 389, 411, 491, 692
BmtI GCTAGC 1 cut(s) 831
BpiI GAAGAC 2 cut(s) 220, 612
BpmI CTGGAG 1 cut(s) 636
BsaBI GATNNNNATC 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 4 cut(s) 598, 810, 1071, 1137
BsaWI WCCGGW 1 cut(s) 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 732
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 149, 190
Bse21I CCTNAGG 1 cut(s) 676
Bse3DI GCAATG 2 cut(s) 116, 180
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 1 cut(s) 1159
BseDI CCNNGG 4 cut(s) 598, 810, 1071, 1137
BseGI GGATG 2 cut(s) 303, 402
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 1 cut(s) 732
BseMI GCAATG 2 cut(s) 116, 180
BseMII CTCAG 2 cut(s) 40, 731
BseNI ACTGG 2 cut(s) 149, 190
BseRI GAGGAG 2 cut(s) 557, 568
BseXI GCAGC 2 cut(s) 139, 937
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 3 cut(s) 503, 527, 1106
Bsh1285I CGRYCG 4 cut(s) 112, 641, 795, 1129
BshFI GGCC 3 cut(s) 94, 189, 494
BshNI GGYRCC 1 cut(s) 233
BsiEI CGRYCG 4 cut(s) 112, 641, 795, 1129
BsiSI CCGG 4 cut(s) 186, 690, 696, 1016
BslFI GGGAC 1 cut(s) 763
BslI CCNNNNNNNGG 1 cut(s) 732
BsmAI GTCTC 3 cut(s) 109, 397, 612
BsmFI GGGAC 1 cut(s) 763
BsnI GGCC 3 cut(s) 94, 189, 494
Bsp143I GATC 5 cut(s) 113, 625, 638, 1029, 1126
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 4 cut(s) 125, 761, 831, 1142
BspANI GGCC 3 cut(s) 94, 189, 494
BspCNI CTCAG 2 cut(s) 39, 730
BspFNI CGCG 3 cut(s) 503, 527, 1106
BspLI GGNNCC 2 cut(s) 235, 694
BspOI GCTAGC 1 cut(s) 831
BspPI GGATC 1 cut(s) 620
BspT107I GGYRCC 1 cut(s) 233
BsrBI CCGCTC 1 cut(s) 761
BsrDI GCAATG 2 cut(s) 116, 180
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 149, 190
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 4 cut(s) 598, 810, 1071, 1137
BssMI GATC 5 cut(s) 113, 625, 638, 1029, 1126
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 3 cut(s) 598, 1071, 1137
Bst2UI CCWGG 1 cut(s) 1159
Bst4CI ACNGT 6 cut(s) 219, 259, 363, 793, 811, 946
Bst6I CTCTTC 2 cut(s) 973, 980
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 5 cut(s) 187, 313, 723, 829, 1008
BstDEI CTNAG 3 cut(s) 26, 676, 717
BstDSI CCRYGG 2 cut(s) 810, 1071
BstENI CCTNNNNNAGG 1 cut(s) 730
BstF5I GGATG 2 cut(s) 303, 402
BstFNI CGCG 3 cut(s) 503, 527, 1106
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 3 cut(s) 132, 236, 529
BstKTI GATC 5 cut(s) 116, 628, 641, 1032, 1129
BstMAI GTCTC 3 cut(s) 109, 397, 612
BstMBI GATC 5 cut(s) 113, 625, 638, 1029, 1126
BstMCI CGRYCG 4 cut(s) 112, 641, 795, 1129
BstMWI GCNNNNNNNGC 3 cut(s) 127, 500, 653
BstNI CCWGG 1 cut(s) 1159
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 1157
BstUI CGCG 3 cut(s) 503, 527, 1106
BstV1I GCAGC 2 cut(s) 139, 937
BstV2I GAAGAC 2 cut(s) 220, 612
BstXI CCANNNNNNTGG 1 cut(s) 1078
Bsu36I CCTNAGG 1 cut(s) 676
BsuRI GGCC 3 cut(s) 94, 189, 494
BtgI CCRYGG 2 cut(s) 810, 1071
BtsCI GGATG 2 cut(s) 303, 402
Cac8I GCNNGC 5 cut(s) 187, 313, 723, 829, 1008
CfoI GCGC 3 cut(s) 132, 236, 529
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 92, 769
Csp6I GTAC 2 cut(s) 220, 263
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 6 cut(s) 101, 286, 766, 906, 921, 1072
CviQI GTAC 2 cut(s) 220, 263
DdeI CTNAG 3 cut(s) 26, 676, 717
DinI GGCGCC 1 cut(s) 235
DpnI GATC 5 cut(s) 115, 627, 640, 1031, 1128
DpnII GATC 5 cut(s) 113, 625, 638, 1029, 1126
DraI TTTAAA 1 cut(s) 1185
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 973, 980
EarI CTCTTC 2 cut(s) 973, 980
EciI GGCGGA 1 cut(s) 1157
Eco130I CCWWGG 3 cut(s) 598, 1071, 1137
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 676
EcoNI CCTNNNNNAGG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 1157
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 3 cut(s) 598, 1071, 1137
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 3 cut(s) 598, 1071, 1137
FaeI CATG 6 cut(s) 104, 289, 769, 909, 924, 1075
FaqI GGGAC 1 cut(s) 763
FatI CATG 6 cut(s) 100, 285, 765, 905, 920, 1071
FauNDI CATATG 1 cut(s) 668
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 2 cut(s) 128, 926
FokI GGATG 2 cut(s) 290, 389
Fsp4HI GCNGC 2 cut(s) 128, 926
FspBI CTAG 4 cut(s) 416, 560, 828, 1022
GlaI GCGC 3 cut(s) 131, 235, 528
GluI GCNGC 2 cut(s) 128, 926
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 3 cut(s) 94, 189, 494
HapII CCGG 4 cut(s) 186, 690, 696, 1016
HhaI GCGC 3 cut(s) 132, 236, 529
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 6 cut(s) 104, 289, 769, 909, 924, 1075
Hin6I GCGC 3 cut(s) 130, 234, 527
HinP1I GCGC 3 cut(s) 130, 234, 527
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 1179
HinfI GANTC 2 cut(s) 156, 611
HpaII CCGG 4 cut(s) 186, 690, 696, 1016
HphI GGTGA 4 cut(s) 351, 562, 614, 866
Hpy166II GTNNAC 4 cut(s) 142, 366, 537, 635
Hpy188I TCNGA 4 cut(s) 109, 731, 818, 1176
Hpy188III TCNNGA 2 cut(s) 14, 615
Hpy8I GTNNAC 4 cut(s) 142, 366, 537, 635
Hpy99I CGWCG 1 cut(s) 1127
HpyAV CCTTC 8 cut(s) 53, 461, 505, 523, 637, 726, 736, 844
HpyCH4III ACNGT 6 cut(s) 219, 259, 363, 793, 811, 946
HpyCH4IV ACGT 2 cut(s) 752, 1164
HpyCH4V TGCA 5 cut(s) 200, 303, 482, 656, 672
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 500, 653
HpyF3I CTNAG 3 cut(s) 26, 676, 717
HpySE526I ACGT 2 cut(s) 752, 1164
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 6 cut(s) 104, 289, 769, 909, 924, 1075
HspAI GCGC 3 cut(s) 130, 234, 527
KasI GGCGCC 1 cut(s) 233
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 5 cut(s) 113, 625, 638, 1029, 1126
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 2 cut(s) 139, 937
LweI GCATC 6 cut(s) 39, 312, 389, 411, 491, 692
MaeI CTAG 4 cut(s) 416, 560, 828, 1022
MaeII ACGT 2 cut(s) 752, 1164
MaeIII GTNAC 2 cut(s) 805, 901
MalI GATC 5 cut(s) 115, 627, 640, 1031, 1128
MbiI CCGCTC 1 cut(s) 761
MboI GATC 5 cut(s) 113, 625, 638, 1029, 1126
MboII GAAGA 8 cut(s) 225, 508, 617, 892, 960, 967, 1024, 1027
MfeI CAATTG 3 cut(s) 195, 488, 657
MluI ACGCGT 2 cut(s) 501, 1104
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 3 cut(s) 132, 796, 919
MnlI CCTC 8 cut(s) 482, 530, 578, 589, 596, 856, 976, 983
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 3 cut(s) 54, 1059, 1184
MspI CCGG 4 cut(s) 186, 690, 696, 1016
MspR9I CCNGG 1 cut(s) 1159
MunI CAATTG 3 cut(s) 195, 488, 657
MvaI CCWGG 1 cut(s) 1159
MvnI CGCG 3 cut(s) 503, 527, 1106
MwoI GCNNNNNNNGC 3 cut(s) 127, 500, 653
NaeI GCCGGC 1 cut(s) 187
NarI GGCGCC 1 cut(s) 234
NcoI CCATGG 1 cut(s) 1071
NdeI CATATG 1 cut(s) 668
NdeII GATC 5 cut(s) 113, 625, 638, 1029, 1126
NgoMIV GCCGGC 1 cut(s) 185
NheI GCTAGC 1 cut(s) 827
NlaIII CATG 6 cut(s) 104, 289, 769, 909, 924, 1075
NlaIV GGNNCC 2 cut(s) 235, 694
NmuCI GTSAC 1 cut(s) 901
NspI RCATGY 1 cut(s) 104
PciI ACATGT 1 cut(s) 100
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 156
PfoI TCCNGGA 1 cut(s) 1157
PkrI GCNGC 2 cut(s) 129, 927
Ple19I CGATCG 2 cut(s) 641, 1129
PleI GAGTC 1 cut(s) 605
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 605
PscI ACATGT 1 cut(s) 100
PsiI TTATAA 1 cut(s) 740
Psp1406I AACGTT 1 cut(s) 1164
Psp6I CCWGG 1 cut(s) 1157
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 1 cut(s) 1157
PspN4I GGNNCC 2 cut(s) 235, 694
PspPI GGNCC 2 cut(s) 92, 769
PvuI CGATCG 2 cut(s) 641, 1129
RsaI GTAC 2 cut(s) 221, 264
RsaNI GTAC 2 cut(s) 220, 263
SaqAI TTAA 3 cut(s) 54, 1059, 1184
SatI GCNGC 2 cut(s) 128, 926
Sau3AI GATC 5 cut(s) 113, 625, 638, 1029, 1126
Sau96I GGNCC 2 cut(s) 92, 769
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 1 cut(s) 1159
SfaNI GCATC 6 cut(s) 39, 312, 389, 411, 491, 692
SfoI GGCGCC 1 cut(s) 235
SinI GGWCC 1 cut(s) 769
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SsiI CCGC 4 cut(s) 125, 761, 831, 1142
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 4 cut(s) 416, 560, 828, 1022
StyD4I CCNGG 1 cut(s) 1157
StyI CCWWGG 3 cut(s) 598, 1071, 1137
TaaI ACNGT 6 cut(s) 219, 259, 363, 793, 811, 946
TaiI ACGT 2 cut(s) 755, 1167
TaqI TCGA 2 cut(s) 641, 1122
TaqII GACCGA 1 cut(s) 126
TatI WGTACW 2 cut(s) 219, 262
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 3 cut(s) 54, 1059, 1184
Tru9I TTAA 3 cut(s) 54, 1059, 1184
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 2 cut(s) 127, 925
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 2 cut(s) 655, 911
TspGWI ACGGA 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 769
XagI CCTNNNNNAGG 1 cut(s) 730
XapI RAATTY 3 cut(s) 267, 372, 1153
XceI RCATGY 1 cut(s) 104
XcmI CCANNNNNNNNNTGG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 4 cut(s) 416, 560, 828, 1022
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.