Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Reverse (-)

26864818 .. 26866635

Gene Structure 1818 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGCTTCTATGTCACCTCTACTCGTCACCTCCACCACAAGTATCATCCCCACAACCTCCCTCTCCCCTTTCTCCCAAAAGTACCACCGAACTTCCTTATTTCGAAACCCTAGGCATTCCAATTTACAAGCTGTCTCATGCAAAGCCACAAATAATAGTAGTGACCAAAATAAAAACCCTTCCACTAGCTCCAACGATCACGACAATGAAAACCCTTCTCCAGTAAACCTAGACAGAAGAAATGTACTCATAGGTCTTGGAGGCCTGTATGGTGGAGTGGCTGGTCTTGGCAGCGACCACTTCGCTTTTGCAAAGCCAGTGGCGCCGCCTGACATAGACAAATGCGGACCTGCGGACTTACCAAGTGGAGCACTCCCAACCAACTGCTGCCCGCCAACGTCCCAAAAAATCGTAGACTTCAAATTCCCCTCCCCTGCCAAACTCCGCGTCAGGCCGGCGGCTCAGTCCGTGGATAAAGCCTACATCGAAAAATACTCAAAAGCCATCGAGCTCATGAAAGCCCTCCCTGACGACGATCCGCGTAGCTTCTCCCAGCAAGCCGATATCCACTGTGCCTATTGCGACGGCGCGTACGATGAAGTCGGCTTCCCCAACCTCGAGCTCCAAATCCATAATTGCTGGCTTTTCTTCCCCTTCCATCGTTACTACCTATACTTCTACGAAAGAATCTTGGGCAAACTCATAGGCGATCCGACGTTCGCGTTGCCGTTTTGGAACTACGACGCGCCAGCTGGCATGCAAATCCCTGCCTTGTACACTAACCCGGACTCTCCACTTTACGACAAGTTCCGCGCTGCCAGCCATCAGCCGCCGACTCTCATCGATCTTGACTTCAACGGCACGGATGAAACAATTTCCAACGATGCTCGAATCGACGCCAACCTCAAACTCATGTATAGGCAGATGATTTCCAACGCCAAGAAACAGCTGTTGTTCTTTGGTGCGCCCTTGAGGGCTGGCACTGAACCAGATCCAGGGCAGGGTTCAATCGAAACGGCCCCACATGGTCCGGTTCATTTATGGACCGGAGATAACACGCAACCTAATATTGAAGACATGGGGAATTTTTACTCCGCTGGAAGGGATCCAATATTTTTTGCGCACCATTCGAATGTGGATCGAATGTGGAATATTTGGAAAAGTTTAGGGACTAAAAATAAAGATATTAACGATCCGGATTGGTTGGATTCGGGGTTCTTGTTTTATGACGAGAATGCTGAGCTTGTTAGGGTCAGGGTGAGGGACTGTCTTGATAATAAAAAGCTAGGGTATACGTATGAAGATGTTGAGATTCCATGGCTCAAGACTAGACCGACGCCACGTCGGACAAAGGCGGCTGGAGTGGCGAAGGCCGCTGAGACGACGTCATTAGGGAAGCTGGTGGCGAGTAAAGATTTTCCAGTAAGTTTGGAGACGAAGATACGTACGGTGGTGCCAAGGCCGAAGCAGAAGAAGAGGAGCAAGAAGGAGAAAGAGGATGAGGAGGAGATATTGGTGATTGATGGGATTGAGTTTGACAAAGATGTGGCTGTGAAGTTTGATGTGTGCGTGAATGACGTGGACGATGATGAGGCACCGAGTGGACCCGTCAATAGCGAGTTTGCTGGGAGTTTTGTGAGTGTGCCACATAAGCACAAGGAAAACAAGAGGAGCAAGGGTTGTTTAAGGTTGGGGTTAACGGACTTGTTGGAGGATTTGGGTGCTGAAGATGATGAGAGTGTGGTGGTGACTTTAGTGCCCAGGTTCGGCACTCAGGCTGTTAAGATCGGTAGCATCAAGATTGAGCTTCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

606

Amino Acids

67.23

Weight (kDa)

5.76

Isoelectric Point (pI)

50.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	181 - 388	2.4e-35	Common central domain of tyrosinase
PPO1_DWL	PF12142	395 - 446	1e-27	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	473 - 603	3.2e-51	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1388
Acc16I	TGCGCA	1 cut(s)	1122
Acc36I	ACCTGC	1 cut(s)	358
AccB1I	GGYRCC	3 cut(s)	322, 1453, 1594
AccI	GTMKAC	2 cut(s)	414, 1292
AccII	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
AccIII	TCCGGA	1 cut(s)	1195
AclWI	GGATC	7 cut(s)	530, 704, 986, 1100, 1113, 1146, 1187
AcsI	RAATTY	2 cut(s)	422, 1084
AcuI	CTGAAG	1 cut(s)	1746
AcyI	GRCGYC	4 cut(s)	323, 897, 1337, 1385
AdeI	CACNNNGTG	1 cut(s)	1601
AfaI	GTAC	5 cut(s)	83, 246, 593, 776, 1447
AfiI	CCNNNNNNNGG	4 cut(s)	995, 1001, 1101, 1766
AgsI	TTSAA	4 cut(s)	421, 856, 1008, 1073
AhdI	GACNNNNNGTC	1 cut(s)	1341
AjiI	CACGTC	2 cut(s)	1343, 1579
AjnI	CCWGG	2 cut(s)	994, 1760
AloI	GAACNNNNNNTCC	2 cut(s)	1199, 1231
Alw21I	GWGCWC	3 cut(s)	373, 513, 624
Alw26I	GTCTC	3 cut(s)	139, 1373, 1427
AlwI	GGATC	7 cut(s)	530, 704, 986, 1100, 1113, 1146, 1187
Ama87I	CYCGRG	1 cut(s)	617
Aor13HI	TCCGGA	1 cut(s)	1195
AoxI	GGCC	5 cut(s)	262, 452, 1017, 1371, 1460
ApeKI	GCWGC	3 cut(s)	291, 387, 815
ApoI	RAATTY	2 cut(s)	422, 1084
ArsI	GACNNNNNNTTYG	2 cut(s)	432, 464
AspA2I	CCTAGG	1 cut(s)	110
AspLEI	GCGC	6 cut(s)	325, 590, 748, 815, 967, 1123
AspS9I	GGNCC	5 cut(s)	347, 1018, 1028, 1044, 1604
AsuC2I	CCSGG	1 cut(s)	785
AsuHPI	GGTGA	5 cut(s)	6, 19, 1270, 1528, 1759
AsuII	TTCGAA	2 cut(s)	103, 1130
AvaI	CYCGRG	1 cut(s)	617
AvaII	GGWCC	4 cut(s)	347, 1028, 1044, 1604
AvrII	CCTAGG	1 cut(s)	110
BaeGI	GKGCMC	1 cut(s)	1761
BamHI	GGATCC	1 cut(s)	1105
BanI	GGYRCC	3 cut(s)	322, 1453, 1594
BanII	GRGCYC	2 cut(s)	513, 624
BbsI	GAAGAC	1 cut(s)	1080
Bbv12I	GWGCWC	3 cut(s)	373, 513, 624
BbvI	GCAGC	3 cut(s)	303, 374, 802
BccI	CCATC	4 cut(s)	512, 666, 831, 1517
BceAI	ACGGC	4 cut(s)	601, 712, 874, 1032
BciT130I	CCWGG	2 cut(s)	996, 1762
BcnI	CCSGG	1 cut(s)	785
BcoDI	GTCTC	3 cut(s)	139, 1373, 1427
BfaI	CTAG	5 cut(s)	111, 186, 230, 1286, 1329
BfoI	RGCGCY	1 cut(s)	326
BfuAI	ACCTGC	1 cut(s)	358
BisI	GCNGC	8 cut(s)	292, 326, 388, 459, 816, 830, 1356, 1374
BlnI	CCTAGG	1 cut(s)	110
BlpI	GCTNAGC	1 cut(s)	1239
BlsI	GCNGC	8 cut(s)	293, 327, 389, 460, 817, 831, 1357, 1375
Bme1390I	CCNGG	3 cut(s)	785, 996, 1762
Bme18I	GGWCC	4 cut(s)	347, 1028, 1044, 1604
BmeRI	GACNNNNNGTC	1 cut(s)	1341
BmeT110I	CYCGRG	1 cut(s)	617
BmgBI	CACGTC	2 cut(s)	1343, 1579
BmgT120I	GGNCC	5 cut(s)	347, 1018, 1028, 1044, 1604
BmiI	GGNNCC	6 cut(s)	324, 1020, 1107, 1455, 1596, 1606
BmrFI	CCNGG	3 cut(s)	785, 996, 1762
BmsI	GCATC	2 cut(s)	874, 1803
BpiI	GAAGAC	1 cut(s)	1080
BpmI	CTGGAG	2 cut(s)	204, 1380
Bpu1102I	GCTNAGC	1 cut(s)	1239
Bpu14I	TTCGAA	2 cut(s)	103, 1130
BpuEI	CTTGAG	2 cut(s)	991, 1307
BpuMI	CCSGG	1 cut(s)	785
Bsa29I	ATCGAT	1 cut(s)	843
BsaAI	YACGTR	2 cut(s)	1296, 1445
BsaHI	GRCGYC	4 cut(s)	323, 897, 1337, 1385
BsaJI	CCNNGG	6 cut(s)	110, 468, 995, 1316, 1457, 1760
BsaWI	WCCGGW	3 cut(s)	1030, 1046, 1195
BsaXI	ACNNNNNCTCC	2 cut(s)	1663, 1693
Bsc4I	CCNNNNNNNGG	4 cut(s)	995, 1001, 1101, 1766
Bse118I	RCCGGY	1 cut(s)	454
Bse1I	ACTGG	3 cut(s)	221, 317, 1421
BseAI	TCCGGA	1 cut(s)	1195
BseBI	CCWGG	2 cut(s)	996, 1762
BseCI	ATCGAT	1 cut(s)	843
BseDI	CCNNGG	6 cut(s)	110, 468, 995, 1316, 1457, 1760
BseGI	GGATG	3 cut(s)	45, 871, 1504
BseLI	CCNNNNNNNGG	4 cut(s)	995, 1001, 1101, 1766
BseMII	CTCAG	4 cut(s)	476, 1230, 1368, 1787
BseNI	ACTGG	3 cut(s)	221, 317, 1421
BseRI	GAGGAG	4 cut(s)	1492, 1517, 1520, 1684
BseSI	GKGCMC	1 cut(s)	1761
BseXI	GCAGC	3 cut(s)	303, 374, 802
BseYI	CCCAGC	2 cut(s)	552, 1625
Bsh1236I	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
BshFI	GGCC	5 cut(s)	264, 454, 1019, 1373, 1462
BshNI	GGYRCC	3 cut(s)	322, 1453, 1594
BshVI	ATCGAT	1 cut(s)	843
BsiHKAI	GWGCWC	3 cut(s)	373, 513, 624
BsiHKCI	CYCGRG	1 cut(s)	617
BsiSI	CCGG	5 cut(s)	455, 785, 1031, 1047, 1196
BsiWI	CGTACG	2 cut(s)	591, 1445
BslFI	GGGAC	3 cut(s)	385, 1183, 1277
BslI	CCNNNNNNNGG	4 cut(s)	995, 1001, 1101, 1766
BsmAI	GTCTC	3 cut(s)	139, 1373, 1427
BsmBI	CGTCTC	2 cut(s)	1373, 1427
BsmFI	GGGAC	3 cut(s)	385, 1183, 1277
BsmI	GAATGC	2 cut(s)	115, 1240
BsnI	GGCC	5 cut(s)	264, 454, 1019, 1373, 1462
BsoBI	CYCGRG	1 cut(s)	617
Bsp119I	TTCGAA	2 cut(s)	103, 1130
Bsp1286I	GDGCHC	4 cut(s)	373, 513, 624, 1761
Bsp13I	TCCGGA	1 cut(s)	1195
Bsp1407I	TGTACA	1 cut(s)	774
Bsp143I	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
Bsp1720I	GCTNAGC	1 cut(s)	1239
Bsp19I	CCATGG	1 cut(s)	1316
BspANI	GGCC	5 cut(s)	264, 454, 1019, 1373, 1462
BspCNI	CTCAG	4 cut(s)	475, 1231, 1369, 1786
BspDI	ATCGAT	1 cut(s)	843
BspEI	TCCGGA	1 cut(s)	1195
BspFNI	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
BspHI	TCATGA	1 cut(s)	513
BspLI	GGNNCC	6 cut(s)	324, 1020, 1107, 1455, 1596, 1606
BspMI	ACCTGC	1 cut(s)	358
BspPI	GGATC	7 cut(s)	530, 704, 986, 1100, 1113, 1146, 1187
BspT104I	TTCGAA	2 cut(s)	103, 1130
BspT107I	GGYRCC	3 cut(s)	322, 1453, 1594
BsrFI	RCCGGY	1 cut(s)	454
BsrGI	TGTACA	1 cut(s)	774
BsrI	ACTGG	3 cut(s)	221, 317, 1421
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	6 cut(s)	110, 468, 995, 1316, 1457, 1760
BssMI	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
BssNAI	GTATAC	1 cut(s)	1293
BssNI	GRCGYC	4 cut(s)	323, 897, 1337, 1385
BssT1I	CCWWGG	3 cut(s)	110, 1316, 1457
Bst1107I	GTATAC	1 cut(s)	1293
Bst2UI	CCWGG	2 cut(s)	996, 1762
Bst4CI	ACNGT	3 cut(s)	572, 1268, 1450
Bst6I	CTCTTC	1 cut(s)	1469
BstACI	GRCGYC	4 cut(s)	323, 897, 1337, 1385
BstAUI	TGTACA	1 cut(s)	774
BstBAI	YACGTR	2 cut(s)	1296, 1445
BstBI	TTCGAA	2 cut(s)	103, 1130
BstC8I	GCNNGC	9 cut(s)	392, 456, 558, 641, 750, 754, 758, 820, 979
BstDEI	CTNAG	4 cut(s)	462, 1239, 1377, 1773
BstDSI	CCRYGG	2 cut(s)	468, 1316
BstF5I	GGATG	3 cut(s)	45, 871, 1504
BstFNI	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
BstH2I	RGCGCY	1 cut(s)	326
BstHHI	GCGC	6 cut(s)	325, 590, 748, 815, 967, 1123
BstKTI	GATC	9 cut(s)	199, 538, 712, 847, 994, 1108, 1141, 1195, 1788
BstMAI	GTCTC	3 cut(s)	139, 1373, 1427
BstMBI	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
BstMWI	GCNNNNNNNGC	5 cut(s)	322, 819, 1364, 1373, 1651
BstNI	CCWGG	2 cut(s)	996, 1762
BstNSI	RCATGY	1 cut(s)	760
BstSCI	CCNGG	3 cut(s)	783, 994, 1760
BstSLI	GKGCMC	1 cut(s)	1761
BstSNI	TACGTA	2 cut(s)	1296, 1445
BstUI	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
BstV1I	GCAGC	3 cut(s)	303, 374, 802
BstV2I	GAAGAC	1 cut(s)	1080
BstX2I	RGATCY	2 cut(s)	991, 1105
BstYI	RGATCY	2 cut(s)	991, 1105
BstZ17I	GTATAC	1 cut(s)	1293
Bsu15I	ATCGAT	1 cut(s)	843
BsuRI	GGCC	5 cut(s)	264, 454, 1019, 1373, 1462
BsuTUI	ATCGAT	1 cut(s)	843
BtgI	CCRYGG	2 cut(s)	468, 1316
BtrI	CACGTC	2 cut(s)	1343, 1579
BtsCI	GGATG	3 cut(s)	45, 871, 1504
BtsIMutI	CAGTG	3 cut(s)	324, 568, 981
BveI	ACCTGC	1 cut(s)	358
Cac8I	GCNNGC	9 cut(s)	392, 456, 558, 641, 750, 754, 758, 820, 979
CciI	TCATGA	1 cut(s)	513
CfoI	GCGC	6 cut(s)	325, 590, 748, 815, 967, 1123
Cfr10I	RCCGGY	1 cut(s)	454
Cfr13I	GGNCC	5 cut(s)	347, 1018, 1028, 1044, 1604
ClaI	ATCGAT	1 cut(s)	843
CseI	GACGC	4 cut(s)	436, 752, 905, 1345
Csp6I	GTAC	5 cut(s)	82, 245, 592, 775, 1446
CspCI	CAANNNNNGTGG	2 cut(s)	300, 335
CviAII	CATG	7 cut(s)	138, 514, 757, 913, 1025, 1078, 1317
CviQI	GTAC	5 cut(s)	82, 245, 592, 775, 1446
DdeI	CTNAG	4 cut(s)	462, 1239, 1377, 1773
DinI	GGCGCC	1 cut(s)	324
DpnI	GATC	9 cut(s)	198, 537, 711, 846, 993, 1107, 1140, 1194, 1787
DpnII	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
DraIII	CACNNNGTG	1 cut(s)	1601
DriI	GACNNNNNGTC	1 cut(s)	1341
Eam1104I	CTCTTC	1 cut(s)	1469
Eam1105I	GACNNNNNGTC	1 cut(s)	1341
EarI	CTCTTC	1 cut(s)	1469
Ecl136II	GAGCTC	2 cut(s)	511, 622
Eco105I	TACGTA	2 cut(s)	1296, 1445
Eco130I	CCWWGG	3 cut(s)	110, 1316, 1457
Eco147I	AGGCCT	1 cut(s)	264
Eco24I	GRGCYC	2 cut(s)	513, 624
Eco32I	GATATC	1 cut(s)	565
Eco47I	GGWCC	4 cut(s)	347, 1028, 1044, 1604
Eco53kI	GAGCTC	2 cut(s)	511, 622
Eco57I	CTGAAG	1 cut(s)	1746
Eco88I	CYCGRG	1 cut(s)	617
EcoICRI	GAGCTC	2 cut(s)	511, 622
EcoRII	CCWGG	2 cut(s)	994, 1760
EcoRV	GATATC	1 cut(s)	565
EcoT14I	CCWWGG	3 cut(s)	110, 1316, 1457
EcoT38I	GRGCYC	2 cut(s)	513, 624
EgeI	GGCGCC	1 cut(s)	324
EheI	GGCGCC	1 cut(s)	324
ErhI	CCWWGG	3 cut(s)	110, 1316, 1457
Esp3I	CGTCTC	2 cut(s)	1373, 1427
FaeI	CATG	7 cut(s)	141, 517, 760, 916, 1028, 1081, 1320
FaqI	GGGAC	3 cut(s)	385, 1183, 1277
FatI	CATG	7 cut(s)	137, 513, 756, 912, 1024, 1077, 1316
FauI	CCCGC	1 cut(s)	399
FblI	GTMKAC	2 cut(s)	414, 1292
Fnu4HI	GCNGC	8 cut(s)	292, 326, 388, 459, 816, 830, 1356, 1374
FokI	GGATG	3 cut(s)	32, 878, 1511
FriOI	GRGCYC	2 cut(s)	513, 624
Fsp4HI	GCNGC	8 cut(s)	292, 326, 388, 459, 816, 830, 1356, 1374
FspBI	CTAG	5 cut(s)	111, 186, 230, 1286, 1329
FspI	TGCGCA	1 cut(s)	1122
GlaI	GCGC	6 cut(s)	324, 589, 747, 814, 966, 1122
GluI	GCNGC	8 cut(s)	292, 326, 388, 459, 816, 830, 1356, 1374
GsaI	CCCAGC	2 cut(s)	556, 1629
GsuI	CTGGAG	2 cut(s)	204, 1380
HaeII	RGCGCY	1 cut(s)	326
HaeIII	GGCC	5 cut(s)	264, 454, 1019, 1373, 1462
HapII	CCGG	5 cut(s)	455, 785, 1031, 1047, 1196
HgaI	GACGC	4 cut(s)	436, 752, 905, 1345
HhaI	GCGC	6 cut(s)	325, 590, 748, 815, 967, 1123
Hin1I	GRCGYC	4 cut(s)	323, 897, 1337, 1385
Hin1II	CATG	7 cut(s)	141, 517, 760, 916, 1028, 1081, 1320
Hin6I	GCGC	6 cut(s)	323, 588, 746, 813, 965, 1121
HinP1I	GCGC	6 cut(s)	323, 588, 746, 813, 965, 1121
HincII	GTYRAC	1 cut(s)	1698
HindII	GTYRAC	1 cut(s)	1698
HinfI	GANTC	6 cut(s)	687, 788, 835, 891, 1208, 1312
HpaI	GTTAAC	1 cut(s)	1698
HpaII	CCGG	5 cut(s)	455, 785, 1031, 1047, 1196
HphI	GGTGA	5 cut(s)	6, 19, 1270, 1528, 1759
Hpy166II	GTNNAC	7 cut(s)	226, 415, 777, 1293, 1582, 1604, 1698
Hpy188I	TCNGA	2 cut(s)	714, 1347
Hpy188III	TCNNGA	7 cut(s)	200, 514, 848, 1196, 1271, 1324, 1798
Hpy8I	GTNNAC	7 cut(s)	226, 415, 777, 1293, 1582, 1604, 1698
Hpy99I	CGWCG	8 cut(s)	536, 587, 718, 746, 899, 1339, 1347, 1387
HpyAV	CCTTC	6 cut(s)	189, 225, 664, 1095, 1363, 1480
HpyCH4III	ACNGT	3 cut(s)	572, 1268, 1450
HpyCH4IV	ACGT	7 cut(s)	398, 716, 1295, 1342, 1385, 1444, 1578
HpyCH4V	TGCA	3 cut(s)	141, 311, 760
HpyF10VI	GCNNNNNNNGC	5 cut(s)	322, 819, 1364, 1373, 1651
HpyF3I	CTNAG	4 cut(s)	462, 1239, 1377, 1773
HpySE526I	ACGT	7 cut(s)	398, 716, 1295, 1342, 1385, 1444, 1578
Hsp92I	GRCGYC	4 cut(s)	323, 897, 1337, 1385
Hsp92II	CATG	7 cut(s)	141, 517, 760, 916, 1028, 1081, 1320
HspAI	GCGC	6 cut(s)	323, 588, 746, 813, 965, 1121
KasI	GGCGCC	1 cut(s)	322
Kpn2I	TCCGGA	1 cut(s)	1195
KroI	GCCGGC	1 cut(s)	454
KroNI	GCCGGC	1 cut(s)	456
KspAI	GTTAAC	1 cut(s)	1698
Kzo9I	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
LmnI	GCTCC	5 cut(s)	194, 368, 627, 1479, 1671
Lsp1109I	GCAGC	3 cut(s)	303, 374, 802
LweI	GCATC	2 cut(s)	874, 1803
MaeI	CTAG	5 cut(s)	111, 186, 230, 1286, 1329
MaeII	ACGT	7 cut(s)	398, 716, 1295, 1342, 1385, 1444, 1578
MaeIII	GTNAC	5 cut(s)	12, 25, 161, 662, 1747
MalI	GATC	9 cut(s)	198, 537, 711, 846, 993, 1107, 1140, 1194, 1787
MboI	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
MboII	GAAGA	8 cut(s)	249, 640, 1085, 1313, 1450, 1483, 1486, 1739
MflI	RGATCY	2 cut(s)	991, 1105
MhlI	GDGCHC	4 cut(s)	373, 513, 624, 1761
MluCI	AATT	5 cut(s)	121, 422, 634, 873, 1084
Mly113I	GGCGCC	1 cut(s)	323
MlyI	GAGTC	2 cut(s)	782, 829
MmeI	TCCRAC	7 cut(s)	216, 737, 903, 957, 1185, 1325, 1689
MroI	TCCGGA	1 cut(s)	1195
MroNI	GCCGGC	1 cut(s)	454
MseI	TTAA	4 cut(s)	1188, 1685, 1697, 1782
MslI	CAYNNNNRTG	2 cut(s)	204, 1131
MspA1I	CMGCKG	4 cut(s)	752, 949, 1097, 1376
MspI	CCGG	5 cut(s)	455, 785, 1031, 1047, 1196
MspR9I	CCNGG	3 cut(s)	785, 996, 1762
Mva1269I	GAATGC	2 cut(s)	115, 1240
MvaI	CCWGG	2 cut(s)	996, 1762
MvnI	CGCG	6 cut(s)	447, 541, 590, 722, 746, 813
MwoI	GCNNNNNNNGC	5 cut(s)	322, 819, 1364, 1373, 1651
NaeI	GCCGGC	1 cut(s)	456
NarI	GGCGCC	1 cut(s)	323
NciI	CCSGG	1 cut(s)	785
NcoI	CCATGG	1 cut(s)	1316
NdeII	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
NgoMIV	GCCGGC	1 cut(s)	454
NlaIII	CATG	7 cut(s)	141, 517, 760, 916, 1028, 1081, 1320
NlaIV	GGNNCC	6 cut(s)	324, 1020, 1107, 1455, 1596, 1606
NmuCI	GTSAC	4 cut(s)	12, 25, 161, 1747
NsbI	TGCGCA	1 cut(s)	1122
NspI	RCATGY	1 cut(s)	760
NspV	TTCGAA	2 cut(s)	103, 1130
PaeI	GCATGC	1 cut(s)	760
PaeR7I	CTCGAG	1 cut(s)	617
PagI	TCATGA	1 cut(s)	513
PceI	AGGCCT	1 cut(s)	264
PcsI	WCGNNNNNNNCGW	1 cut(s)	591
PctI	GAATGC	2 cut(s)	115, 1240
PdiI	GCCGGC	1 cut(s)	456
PfeI	GAWTC	4 cut(s)	687, 891, 1208, 1312
Pfl23II	CGTACG	2 cut(s)	591, 1445
PflFI	GACNNNGTC	1 cut(s)	1384
PkrI	GCNGC	8 cut(s)	293, 327, 389, 460, 817, 831, 1357, 1375
PleI	GAGTC	2 cut(s)	782, 829
PluTI	GGCGCC	1 cut(s)	326
PpsI	GAGTC	2 cut(s)	782, 829
Ppu21I	YACGTR	2 cut(s)	1296, 1445
Psp124BI	GAGCTC	2 cut(s)	513, 624
Psp6I	CCWGG	2 cut(s)	994, 1760
PspFI	CCCAGC	2 cut(s)	552, 1625
PspGI	CCWGG	2 cut(s)	994, 1760
PspLI	CGTACG	2 cut(s)	591, 1445
PspN4I	GGNNCC	6 cut(s)	324, 1020, 1107, 1455, 1596, 1606
PspPI	GGNCC	5 cut(s)	347, 1018, 1028, 1044, 1604
PspXI	VCTCGAGB	1 cut(s)	617
PsuI	RGATCY	2 cut(s)	991, 1105
PsyI	GACNNNGTC	1 cut(s)	1384
PvuII	CAGCTG	2 cut(s)	752, 949
RsaI	GTAC	5 cut(s)	83, 246, 593, 776, 1447
RsaNI	GTAC	5 cut(s)	82, 245, 592, 775, 1446
RseI	CAYNNNNRTG	2 cut(s)	204, 1131
SacI	GAGCTC	2 cut(s)	513, 624
SaqAI	TTAA	4 cut(s)	1188, 1685, 1697, 1782
SatI	GCNGC	8 cut(s)	292, 326, 388, 459, 816, 830, 1356, 1374
Sau3AI	GATC	9 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1192, 1785
Sau96I	GGNCC	5 cut(s)	347, 1018, 1028, 1044, 1604
SchI	GAGTC	2 cut(s)	782, 829
ScrFI	CCNGG	3 cut(s)	785, 996, 1762
SduI	GDGCHC	4 cut(s)	373, 513, 624, 1761
SfaNI	GCATC	2 cut(s)	874, 1803
SfoI	GGCGCC	1 cut(s)	324
Sfr274I	CTCGAG	1 cut(s)	617
SfuI	TTCGAA	2 cut(s)	103, 1130
SinI	GGWCC	4 cut(s)	347, 1028, 1044, 1604
SlaI	CTCGAG	1 cut(s)	617
SmiMI	CAYNNNNRTG	2 cut(s)	204, 1131
SmlI	CTYRAG	3 cut(s)	617, 970, 1322
SmoI	CTYRAG	3 cut(s)	617, 970, 1322
SnaBI	TACGTA	2 cut(s)	1296, 1445
SphI	GCATGC	1 cut(s)	760
Sse9I	AATT	5 cut(s)	121, 422, 634, 873, 1084
SseBI	AGGCCT	1 cut(s)	264
SspDI	GGCGCC	1 cut(s)	322
SspI	AATATT	3 cut(s)	1069, 1113, 1153
SspMI	CTAG	5 cut(s)	111, 186, 230, 1286, 1329
SstI	GAGCTC	2 cut(s)	513, 624
StuI	AGGCCT	1 cut(s)	264
StyD4I	CCNGG	3 cut(s)	783, 994, 1760
StyI	CCWWGG	3 cut(s)	110, 1316, 1457
TaaI	ACNGT	3 cut(s)	572, 1268, 1450
TaiI	ACGT	7 cut(s)	401, 719, 1298, 1345, 1388, 1447, 1581
TaqII	GACCGA	1 cut(s)	1348
TasI	AATT	5 cut(s)	121, 422, 634, 873, 1084
TatI	WGTACW	2 cut(s)	244, 774
TauI	GCSGC	5 cut(s)	328, 461, 832, 1358, 1376
TfiI	GAWTC	4 cut(s)	687, 891, 1208, 1312
Tru1I	TTAA	4 cut(s)	1188, 1685, 1697, 1782
Tru9I	TTAA	4 cut(s)	1188, 1685, 1697, 1782
TscAI	CASTG	3 cut(s)	324, 575, 988
TseFI	GTSAC	4 cut(s)	12, 25, 161, 1747
TseI	GCWGC	3 cut(s)	291, 387, 815
Tsp45I	GTSAC	4 cut(s)	12, 25, 161, 1747
TspDTI	ATGAA	6 cut(s)	222, 530, 612, 882, 1025, 1314
TspGWI	ACGGA	3 cut(s)	457, 878, 1715
TspRI	CASTG	3 cut(s)	324, 575, 988
Tth111I	GACNNNGTC	1 cut(s)	1384
VpaK11BI	GGWCC	4 cut(s)	347, 1028, 1044, 1604
XapI	RAATTY	2 cut(s)	422, 1084
XceI	RCATGY	1 cut(s)	760
XhoI	CTCGAG	1 cut(s)	617
XmaJI	CCTAGG	1 cut(s)	110
XmiI	GTMKAC	2 cut(s)	414, 1292
XspI	CTAG	5 cut(s)	111, 186, 230, 1286, 1329
ZraI	GACGTC	1 cut(s)	1386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.