pycom13g14280

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002996
Physical Location & Seq
Reverse (-)
10299480 .. 10305011
5532 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1443 bp
ATGCCCGGGCCCACCTTGGAGATGGAACCAGTGGAGCCACAGTCGCTGAAGAAGCTCAGTCTCAAATCCCTCAAACGATCCCTCGATCTCTTCTCCCCCGCCCACGGCGAACTCCCCCCTCCCGACCCCGAGAGTAAGAGAATTCGCATGAGTCACAAGATAAGCATGGAATATGGAGGAATTCAACCCACACCTAGCCAAAAACCACCTCGCCGGGCTGAGTCGGGTGCACCAGTAACTGGTTCTGAAGGGCCTTCCAATGCCCTTGCCCTTACAGATTCTAAGGATGCGCAGACGGGAGGAGCTCAAAACTCTTTGGTTGTCGGTCCTTCTGCACCAAGGGCACTGAATACTGTTGGTATTTCGGGTAAAGGCACTTTAGCTGTTTCTGCTCCAGGGTCATCTGAAAGGTTTTCAACATCGGCCATGATGGAAAGGATTTCCAGCAGATGGCCACGTCCTGATTGGCATGCTCCTTGGAAAATCTACAGAGTCATTAGCGGCCACTTAGGATGGGTACGAGCTATTGCCTTTGATCCAAGTAATAAATGGTTCTGCACTGGCTCAGCAGACCGTACCATCAAGATTTGGGATGTTGCAACTGGAACTCTACAACTGACAGTGACAGGACACATCGAGCAAATACGAGGCCTTGCGGTTAGCAGCAGGCATACCTATATGTTCTCTGCTGGAGATGACAAACTAGTTAAATGCTGGGATCTTGAACAGAATAAGGTTATCCGTTCATATCATGGTCATCTAAGTGGTGTTTACTGCTTGGCTCTTCATCCCACTCTTGATGTTTTACTTACCGGGGGACGTGATTCTGTATGCCGGGTTTGGGATATTCGAAGCAAGATGCAAGTTCATGCGTTGTCGGGGCATGACAACACAGTTTGTTCTGTTTTTACTCGACCAACAGATCCACAAGTTGTTACTGGCTCTCATGACACAACCATCAAGTTCTGGGACCTTAGATATGGAAAAACAATGTCAACTCTGACACACCATAAAAAGTCTGTGCGTGCAATGGCCCAGCATCCTAAAGAGCAATGTTTTGCATCTGCATCAGCTGATAATATTAAGAAGTTCAGCCTCCCAAGAGGCGAATTTTTACACAACATGCTCTCTCAGCAGAAAACAATAATCAATGCAATGGCTGTCAACGAGGATGGAGTTATGGCCACTGGAGGCGATAATGGGAGCATGTGGTTCTGGGATTGGAAGAGCGGTCACAATTTTCAGCAATCCCAAACAATTGTACAGCCTGGCTCGCTGGATAGTGAAGCTGGTATATATGCTCTTTCATACGACATCACGGGTACGAGGCTGGTCAGTTGTGAAGCCGATAAGACTATCAAGATGTGGAGGCAAGATGAAAATGCCACTCCAGAAACCCACCCTCTCAACTTTAGGCCCCCGAAAGACATTCGACGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

481

Amino Acids

53.03

Weight (kDa)

9.18

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 126 - 259 1.3e-07 TEP-1 second beta-propeller
WD40_Gbeta PF25391 147 - 302 6.1e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR41 PF25178 157 - 304 3.1e-11 WDR41
Beta-prop_THOC3 PF25174 160 - 265 3.3e-18 THOC3 beta-propeller domain
WD40_Prp19 PF24814 161 - 299 3.9e-23 Prp19 WD40 domain
WD40 PF00400 161 - 198 6.8e-11 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 162 - 282 7.8e-19 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 163 - 273 6.5e-14 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 163 - 241 3.4e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 165 - 283 2.4e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 166 - 340 3.8e-41 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 173 - 249 8.4e-11 WDHD1 first WD40 domain
WDR55 PF24796 174 - 330 7.1e-17 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 175 - 245 1.5e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 180 - 361 3.2e-16 WDR36/Utp21 first beta-propeller
EIF3I PF24805 207 - 291 4.3e-06 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 209 - 361 9.5e-16 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 211 - 415 7e-28 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 211 - 323 6.1e-07 EIPR1 beta-propeller
WD40_RFWD3 PF23419 211 - 302 2.9e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_CDC20-Fz PF24807 221 - 456 3.2e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 242 - 360 1.4e-24 WDR3 first beta-propeller domain
WD40 PF00400 245 - 282 2.1e-08 WD domain, G-beta repeat
Beta-prop_EML PF23409 248 - 465 7.8e-16 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 253 - 454 2.1e-07 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 255 - 456 1.8e-29 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 257 - 418 7.3e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 257 - 417 1.7e-14 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 267 - 348 2e-08 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 268 - 415 1.8e-06 SCAP Beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 288 - 363 2e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 288 - 324 1.6e-06 WD domain, G-beta repeat
EIF3I PF24805 291 - 418 6.2e-06 EIF3I
Beta-prop_EML_2 PF23414 303 - 457 1e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 307 - 410 4.8e-07 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 308 - 456 3.5e-08 G protein beta WD-40 repeat protein
WDR55 PF24796 330 - 415 3e-07 WDR55
Beta-prop_WDR5 PF25175 333 - 461 5.5e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 291
AccB7I CCANNNNNTGG 2 cut(s) 239, 450
AccBSI CCGCTC 1 cut(s) 1230
AciI CCGC 4 cut(s) 99, 501, 656, 1230
AclWI GGATC 4 cut(s) 72, 530, 726, 917
AcoI YGGCCR 4 cut(s) 423, 452, 502, 1182
AcsI RAATTY 3 cut(s) 141, 180, 1109
AcuI CTGAAG 2 cut(s) 68, 267
AfaI GTAC 4 cut(s) 519, 577, 1263, 1324
AfiI CCNNNNNNNGG 4 cut(s) 104, 239, 450, 841
AgsI TTSAA 3 cut(s) 185, 417, 725
AhlI ACTAGT 1 cut(s) 703
AjiI CACGTC 2 cut(s) 458, 821
AjnI CCWGG 2 cut(s) 394, 1267
AluBI AGCT 6 cut(s) 55, 305, 383, 524, 1073, 1289
AluI AGCT 6 cut(s) 55, 305, 383, 524, 1073, 1289
Alw21I GWGCWC 2 cut(s) 232, 307
Alw26I GTCTC 1 cut(s) 65
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 4 cut(s) 72, 530, 726, 917
AlwNI CAGNNNCTG 2 cut(s) 46, 239
Ama87I CYCGRG 2 cut(s) 5, 128
AoxI GGCC 9 cut(s) 8, 251, 423, 452, 502, 649, 1032, 1182, 1415
ApaI GGGCCC 1 cut(s) 12
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 663
ApoI RAATTY 3 cut(s) 141, 180, 1109
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 7 cut(s) 8, 9, 251, 326, 970, 1033, 1416
AsuC2I CCSGG 5 cut(s) 6, 7, 215, 814, 836
AsuII TTCGAA 1 cut(s) 850
AvaI CYCGRG 2 cut(s) 5, 128
AvaII GGWCC 2 cut(s) 326, 970
BaeGI GKGCMC 3 cut(s) 12, 232, 346
BalI TGGCCA 2 cut(s) 454, 1184
BanII GRGCYC 2 cut(s) 12, 307
Bbv12I GWGCWC 2 cut(s) 232, 307
BbvI GCAGC 1 cut(s) 675
BccI CCATC 7 cut(s) 16, 424, 444, 507, 587, 965, 1166
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 396, 1269
BcnI CCSGG 5 cut(s) 6, 7, 215, 814, 836
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 703
BfaI CTAG 2 cut(s) 195, 704
BfmI CTRYAG 1 cut(s) 487
BisI GCNGC 2 cut(s) 502, 664
BlpI GCTNAGC 1 cut(s) 565
BlsI GCNGC 2 cut(s) 503, 665
Bme1390I CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1269
Bme18I GGWCC 2 cut(s) 326, 970
BmeT110I CYCGRG 2 cut(s) 5, 128
BmgBI CACGTC 2 cut(s) 458, 821
BmgT120I GGNCC 7 cut(s) 8, 9, 251, 326, 970, 1033, 1416
BmiI GGNNCC 5 cut(s) 10, 27, 36, 971, 1418
BmrFI CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1269
BmsI GCATC 5 cut(s) 277, 849, 1048, 1070, 1076
BpmI CTGGAG 4 cut(s) 378, 711, 1209, 1374
Bpu1102I GCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 1 cut(s) 850
BpuMI CCSGG 5 cut(s) 6, 7, 215, 814, 836
BsaJI CCNNGG 7 cut(s) 5, 15, 103, 338, 395, 476, 813
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 239, 450, 841
Bse1I ACTGG 7 cut(s) 29, 233, 244, 565, 607, 943, 1192
Bse3DI GCAATG 3 cut(s) 1035, 1058, 1161
BseBI CCWGG 2 cut(s) 396, 1269
BseDI CCNNGG 7 cut(s) 5, 15, 103, 338, 395, 476, 813
BseGI GGATG 6 cut(s) 292, 518, 598, 787, 1039, 1177
BseLI CCNNNNNNNGG 4 cut(s) 104, 239, 450, 841
BseMI GCAATG 3 cut(s) 1035, 1058, 1161
BseMII CTCAG 4 cut(s) 70, 210, 579, 1145
BseNI ACTGG 7 cut(s) 29, 233, 244, 565, 607, 943, 1192
BseRI GAGGAG 1 cut(s) 315
BseSI GKGCMC 3 cut(s) 12, 232, 346
BseXI GCAGC 1 cut(s) 675
BseYI CCCAGC 2 cut(s) 714, 1035
BsgI GTGCAG 2 cut(s) 318, 541
BshFI GGCC 9 cut(s) 10, 253, 425, 454, 504, 651, 1034, 1184, 1417
BsiHKAI GWGCWC 2 cut(s) 232, 307
BsiHKCI CYCGRG 2 cut(s) 5, 128
BsiSI CCGG 4 cut(s) 6, 214, 813, 835
BslFI GGGAC 2 cut(s) 831, 983
BslI CCNNNNNNNGG 4 cut(s) 104, 239, 450, 841
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 831, 983
BsnI GGCC 9 cut(s) 10, 253, 425, 454, 504, 651, 1034, 1184, 1417
BsoBI CYCGRG 2 cut(s) 5, 128
Bsp119I TTCGAA 1 cut(s) 850
Bsp120I GGGCCC 1 cut(s) 8
Bsp1286I GDGCHC 4 cut(s) 12, 232, 307, 346
Bsp1407I TGTACA 1 cut(s) 1261
Bsp143I GATC 5 cut(s) 77, 85, 535, 718, 922
Bsp1720I GCTNAGC 1 cut(s) 565
BspACI CCGC 4 cut(s) 99, 501, 656, 1230
BspANI GGCC 9 cut(s) 10, 253, 425, 454, 504, 651, 1034, 1184, 1417
BspCNI CTCAG 4 cut(s) 69, 211, 578, 1144
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 5 cut(s) 10, 27, 36, 971, 1418
BspPI GGATC 4 cut(s) 72, 530, 726, 917
BspQI GCTCTTC 2 cut(s) 789, 1220
BspT104I TTCGAA 1 cut(s) 850
BsrBI CCGCTC 1 cut(s) 1230
BsrDI GCAATG 3 cut(s) 1035, 1058, 1161
BsrGI TGTACA 1 cut(s) 1261
BsrI ACTGG 7 cut(s) 29, 233, 244, 565, 607, 943, 1192
BssECI CCNNGG 7 cut(s) 5, 15, 103, 338, 395, 476, 813
BssMI GATC 5 cut(s) 77, 85, 535, 718, 922
BssT1I CCWWGG 3 cut(s) 15, 338, 476
Bst2UI CCWGG 2 cut(s) 396, 1269
Bst4CI ACNGT 6 cut(s) 42, 355, 575, 622, 895, 1437
Bst6I CTCTTC 3 cut(s) 95, 789, 1220
BstAUI TGTACA 1 cut(s) 1261
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 471, 668, 1026, 1274
BstDEI CTNAG 8 cut(s) 56, 219, 282, 508, 565, 761, 974, 1131
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 6 cut(s) 292, 518, 598, 787, 1039, 1177
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 5 cut(s) 80, 88, 538, 721, 925
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 5 cut(s) 77, 85, 535, 718, 922
BstMWI GCNNNNNNNGC 6 cut(s) 43, 52, 341, 389, 1132, 1273
BstNI CCWGG 2 cut(s) 396, 1269
BstNSI RCATGY 3 cut(s) 473, 1126, 1210
BstSCI CCNGG 7 cut(s) 4, 5, 213, 394, 812, 834, 1267
BstSFI CTRYAG 1 cut(s) 487
BstSLI GKGCMC 3 cut(s) 12, 232, 346
BstV1I GCAGC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 718, 922
BstYI RGATCY 2 cut(s) 718, 922
BsuRI GGCC 9 cut(s) 10, 253, 425, 454, 504, 651, 1034, 1184, 1417
BtgI CCRYGG 1 cut(s) 103
BtrI CACGTC 2 cut(s) 458, 821
BtsCI GGATG 6 cut(s) 292, 518, 598, 787, 1039, 1177
BtsIMutI CAGTG 5 cut(s) 36, 344, 558, 627, 1185
Cac8I GCNNGC 4 cut(s) 471, 668, 1026, 1274
CaiI CAGNNNCTG 2 cut(s) 46, 239
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 7 cut(s) 8, 9, 251, 326, 970, 1033, 1416
Cfr9I CCCGGG 1 cut(s) 5
Csp6I GTAC 4 cut(s) 518, 576, 1262, 1323
CviQI GTAC 4 cut(s) 518, 576, 1262, 1323
DdeI CTNAG 8 cut(s) 56, 219, 282, 508, 565, 761, 974, 1131
DpnI GATC 5 cut(s) 79, 87, 537, 720, 924
DpnII GATC 5 cut(s) 77, 85, 535, 718, 922
EaeI YGGCCR 4 cut(s) 423, 452, 502, 1182
Eam1104I CTCTTC 3 cut(s) 95, 789, 1220
EarI CTCTTC 3 cut(s) 95, 789, 1220
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 3 cut(s) 15, 338, 476
Eco147I AGGCCT 1 cut(s) 651
Eco24I GRGCYC 2 cut(s) 12, 307
Eco47I GGWCC 2 cut(s) 326, 970
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 68, 267
Eco88I CYCGRG 2 cut(s) 5, 128
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 3 cut(s) 251, 970, 1416
EcoRI GAATTC 2 cut(s) 141, 180
EcoRII CCWGG 2 cut(s) 394, 1267
EcoT14I CCWWGG 3 cut(s) 15, 338, 476
EcoT38I GRGCYC 2 cut(s) 12, 307
ErhI CCWWGG 3 cut(s) 15, 338, 476
FaqI GGGAC 2 cut(s) 831, 983
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 502, 664
FokI GGATG 6 cut(s) 299, 525, 605, 774, 1026, 1184
FriOI GRGCYC 2 cut(s) 12, 307
Fsp4HI GCNGC 2 cut(s) 502, 664
FspBI CTAG 2 cut(s) 195, 704
FspI TGCGCA 1 cut(s) 291
GlaI GCGC 1 cut(s) 291
GluI GCNGC 2 cut(s) 502, 664
GsaI CCCAGC 2 cut(s) 718, 1039
GsuI CTGGAG 4 cut(s) 378, 711, 1209, 1374
HaeIII GGCC 9 cut(s) 10, 253, 425, 454, 504, 651, 1034, 1184, 1417
HapII CCGG 4 cut(s) 6, 214, 813, 835
HhaI GCGC 1 cut(s) 292
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 2 cut(s) 996, 1165
HindII GTYRAC 2 cut(s) 996, 1165
HinfI GANTC 5 cut(s) 151, 221, 278, 492, 824
HpaII CCGG 4 cut(s) 6, 214, 813, 835
Hpy166II GTNNAC 4 cut(s) 230, 772, 996, 1165
Hpy188I TCNGA 3 cut(s) 247, 406, 1002
Hpy188III TCNNGA 8 cut(s) 122, 461, 583, 722, 797, 947, 1360, 1391
Hpy8I GTNNAC 4 cut(s) 230, 772, 996, 1165
Hpy99I CGWCG 1 cut(s) 1437
HpyAV CCTTC 3 cut(s) 242, 264, 339
HpyCH4III ACNGT 6 cut(s) 42, 355, 575, 622, 895, 1437
HpyCH4IV ACGT 2 cut(s) 457, 820
HpyCH4V TGCA 9 cut(s) 230, 335, 558, 599, 862, 1028, 1061, 1067, 1154
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 52, 341, 389, 1132, 1273
HpyF3I CTNAG 8 cut(s) 56, 219, 282, 508, 565, 761, 974, 1131
HpySE526I ACGT 2 cut(s) 457, 820
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 5 cut(s) 77, 85, 535, 718, 922
LguI GCTCTTC 2 cut(s) 789, 1220
LmnI GCTCC 5 cut(s) 34, 302, 397, 478, 1203
Lsp1109I GCAGC 1 cut(s) 675
LweI GCATC 5 cut(s) 277, 849, 1048, 1070, 1076
MaeI CTAG 2 cut(s) 195, 704
MaeII ACGT 2 cut(s) 457, 820
MaeIII GTNAC 5 cut(s) 152, 235, 622, 934, 1232
MalI GATC 5 cut(s) 79, 87, 537, 720, 924
MbiI CCGCTC 1 cut(s) 1230
MboI GATC 5 cut(s) 77, 85, 535, 718, 922
MboII GAAGA 4 cut(s) 61, 82, 776, 1237
MfeI CAATTG 1 cut(s) 1257
MflI RGATCY 2 cut(s) 718, 922
MhlI GDGCHC 4 cut(s) 12, 232, 307, 346
MlsI TGGCCA 2 cut(s) 454, 1184
MluCI AATT 5 cut(s) 141, 180, 1109, 1237, 1257
MluNI TGGCCA 2 cut(s) 454, 1184
MlyI GAGTC 3 cut(s) 160, 230, 501
Mox20I TGGCCA 2 cut(s) 454, 1184
MscI TGGCCA 2 cut(s) 454, 1184
MseI TTAA 2 cut(s) 708, 1083
MslI CAYNNNNRTG 1 cut(s) 762
Msp20I TGGCCA 2 cut(s) 454, 1184
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 4 cut(s) 6, 214, 813, 835
MspR9I CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1269
MunI CAATTG 1 cut(s) 1257
MvaI CCWGG 2 cut(s) 396, 1269
MwoI GCNNNNNNNGC 6 cut(s) 43, 52, 341, 389, 1132, 1273
NciI CCSGG 5 cut(s) 6, 7, 215, 814, 836
NdeII GATC 5 cut(s) 77, 85, 535, 718, 922
NlaIV GGNNCC 5 cut(s) 10, 27, 36, 971, 1418
NmuCI GTSAC 3 cut(s) 152, 622, 1232
NsbI TGCGCA 1 cut(s) 291
NspI RCATGY 3 cut(s) 473, 1126, 1210
NspV TTCGAA 1 cut(s) 850
PaeI GCATGC 1 cut(s) 473
PagI TCATGA 1 cut(s) 946
PceI AGGCCT 1 cut(s) 651
PciSI GCTCTTC 2 cut(s) 789, 1220
PfeI GAWTC 2 cut(s) 278, 824
PflMI CCANNNNNTGG 2 cut(s) 239, 450
PkrI GCNGC 2 cut(s) 503, 665
PleI GAGTC 3 cut(s) 159, 229, 500
PpsI GAGTC 3 cut(s) 159, 229, 500
PpuMI RGGWCCY 1 cut(s) 970
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 2 cut(s) 394, 1267
PspFI CCCAGC 2 cut(s) 714, 1035
PspGI CCWGG 2 cut(s) 394, 1267
PspN4I GGNNCC 5 cut(s) 10, 27, 36, 971, 1418
PspOMI GGGCCC 1 cut(s) 8
PspPI GGNCC 7 cut(s) 8, 9, 251, 326, 970, 1033, 1416
PspPPI RGGWCCY 1 cut(s) 970
PstNI CAGNNNCTG 2 cut(s) 46, 239
PsuI RGATCY 2 cut(s) 718, 922
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 4 cut(s) 519, 577, 1263, 1324
RsaNI GTAC 4 cut(s) 518, 576, 1262, 1323
RseI CAYNNNNRTG 1 cut(s) 762
SacI GAGCTC 1 cut(s) 307
SapI GCTCTTC 2 cut(s) 789, 1220
SaqAI TTAA 2 cut(s) 708, 1083
SatI GCNGC 2 cut(s) 502, 664
Sau3AI GATC 5 cut(s) 77, 85, 535, 718, 922
Sau96I GGNCC 7 cut(s) 8, 9, 251, 326, 970, 1033, 1416
SchI GAGTC 3 cut(s) 160, 230, 501
ScrFI CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1269
SduI GDGCHC 4 cut(s) 12, 232, 307, 346
SfaNI GCATC 5 cut(s) 277, 849, 1048, 1070, 1076
SfcI CTRYAG 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 850
SinI GGWCC 2 cut(s) 326, 970
SmaI CCCGGG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 762
SpeI ACTAGT 1 cut(s) 703
SphI GCATGC 1 cut(s) 473
SrfI GCCCGGGC 1 cut(s) 7
Sse9I AATT 5 cut(s) 141, 180, 1109, 1237, 1257
SseBI AGGCCT 1 cut(s) 651
SsiI CCGC 4 cut(s) 99, 501, 656, 1230
SspI AATATT 1 cut(s) 1081
SspMI CTAG 2 cut(s) 195, 704
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 651
StyD4I CCNGG 7 cut(s) 4, 5, 213, 394, 812, 834, 1267
StyI CCWWGG 3 cut(s) 15, 338, 476
TaaI ACNGT 6 cut(s) 42, 355, 575, 622, 895, 1437
TaiI ACGT 2 cut(s) 460, 823
TaqI TCGA 5 cut(s) 84, 636, 850, 913, 1432
TaqII GACCGA 1 cut(s) 314
TasI AATT 5 cut(s) 141, 180, 1109, 1237, 1257
TatI WGTACW 1 cut(s) 1261
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 278, 824
Tru1I TTAA 2 cut(s) 708, 1083
Tru9I TTAA 2 cut(s) 708, 1083
TscAI CASTG 5 cut(s) 36, 351, 565, 627, 1192
TseFI GTSAC 3 cut(s) 152, 622, 1232
TseI GCWGC 1 cut(s) 663
Tsp45I GTSAC 3 cut(s) 152, 622, 1232
TspDTI ATGAA 5 cut(s) 735, 776, 857, 1296, 1392
TspGWI ACGGA 1 cut(s) 731
TspMI CCCGGG 1 cut(s) 5
TspRI CASTG 5 cut(s) 36, 351, 565, 627, 1192
Van91I CCANNNNNTGG 2 cut(s) 239, 450
VneI GTGCAC 1 cut(s) 228
VpaK11BI GGWCC 2 cut(s) 326, 970
XapI RAATTY 3 cut(s) 141, 180, 1109
XceI RCATGY 3 cut(s) 473, 1126, 1210
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 462, 546
XmaI CCCGGG 1 cut(s) 5
XspI CTAG 2 cut(s) 195, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.