pycom13g16130

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002958
Physical Location & Seq
Reverse (-)
11863834 .. 11866129
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGGGAATTGATCTGGAATCAGTTGCAGAGGCAACGTCCGGCGCCATTGGAGCTCTGGTCAGCACCACCATCCTGTACCCTCTTGACACCTGCAAGACCAAGTACCAAGCTGAAGTCCGAGCCCACCATCAGCAGAAATACAGGAACATTTCTGATGTGTTATGGGAAGCAATTTCTACCCATCAGGTGCTTTCTTTGTACCAGGGCCTTGGGACAAAGAACTTTCAGTCCTTTATTTCCCAGTTCATCTACTTCTATGGATACAGTTTTTTTAAGAGGTTATACTTGGAGAAAAGTGGAAATAAAAACATGGGAACAAGAGCAAACTTGATGGTTGCAGCCGCTGCCGGGGCTTGTACAGTCATAGTGACACAGCCCTTGGATACAGCATCCTCAAAGATGCAGACAAGTGAGTTTGGGAAATCCAAAGGACTCTGGAAGACGCTTTCAGAGGGGACGTGGATTGAGGCATTTGACGGTCTTGGCATATCTCTTCTTTTGACATCTAACCCATCTATCCAGTACACAGTATTTGATCGAGCTCAAACAAAGGCTTTTGAGAGGACAGCTGAGCAAAAGAACAGGTACAAAGTCATCTCCAGAAGCTCTTTCTGCCCTTTCTGCTTTCGTATTGGGTGCTGTCTCAAAGTGTATTGCATCCTGCTTGACATACCCAGCCATCAGGTGCAAGGTCATGATTCAAGCTGCAGAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

240

Amino Acids

26.77

Weight (kDa)

9.16

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 6 - 98 7e-16 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 41
AciI CCGC 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 6 cut(s) 77, 104, 200, 358, 524, 587
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 1 cut(s) 702
AjiI CACGTC 1 cut(s) 459
AjnI CCWGG 1 cut(s) 201
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
AluBI AGCT 6 cut(s) 53, 110, 542, 569, 606, 705
AluI AGCT 6 cut(s) 53, 110, 542, 569, 606, 705
Alw21I GWGCWC 2 cut(s) 55, 544
Alw26I GTCTC 1 cut(s) 647
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 338, 344, 705
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 349
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 3 cut(s) 55, 124, 544
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 55, 544
BbvI GCAGC 3 cut(s) 331, 350, 692
BccI CCATC 6 cut(s) 77, 135, 189, 325, 520, 687
BciT130I CCWGG 1 cut(s) 203
BciVI GTATCC 2 cut(s) 254, 376
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 706
BfoI RGCGCY 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 98
BfuI GTATCC 2 cut(s) 254, 376
BisI GCNGC 4 cut(s) 339, 342, 345, 706
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 4 cut(s) 340, 343, 346, 707
Bme1390I CCNGG 2 cut(s) 203, 349
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 2 cut(s) 203, 349
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 3 cut(s) 390, 398, 666
BmuI ACTGGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 583
Bpu1102I GCTNAGC 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 202, 208, 348, 378
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse1I ACTGG 2 cut(s) 241, 520
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 4 cut(s) 202, 208, 348, 378
BseGI GGATG 3 cut(s) 69, 389, 657
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 561
BseNI ACTGG 2 cut(s) 241, 520
BseXI GCAGC 3 cut(s) 331, 350, 692
BseYI CCCAGC 1 cut(s) 674
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 55, 544
BsiSI CCGG 2 cut(s) 39, 348
BslFI GGGAC 2 cut(s) 226, 469
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 1 cut(s) 647
BsmFI GGGAC 2 cut(s) 226, 469
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 3 cut(s) 55, 124, 544
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 2 cut(s) 10, 535
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 1 cut(s) 342
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 562
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 710
BspMI ACCTGC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 2 cut(s) 241, 520
BssECI CCNNGG 4 cut(s) 202, 208, 348, 378
BssMI GATC 2 cut(s) 10, 535
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 208, 378
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 4 cut(s) 266, 361, 479, 529
Bst6I CTCTTC 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 570
BstF5I GGATG 3 cut(s) 69, 389, 657
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 13, 538
BstMAI GTCTC 1 cut(s) 647
BstMBI GATC 2 cut(s) 10, 535
BstMWI GCNNNNNNNGC 5 cut(s) 50, 344, 350, 612, 621
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 201, 347
BstSFI CTRYAG 1 cut(s) 706
BstV1I GCAGC 3 cut(s) 331, 350, 692
BstV2I GAAGAC 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 209
BsuI GTATCC 2 cut(s) 254, 376
BsuRI GGCC 1 cut(s) 207
BtrI CACGTC 1 cut(s) 459
BtsCI GGATG 3 cut(s) 69, 389, 657
BveI ACCTGC 1 cut(s) 98
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 1 cut(s) 694
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 1 cut(s) 451
Csp6I GTAC 6 cut(s) 76, 103, 199, 357, 523, 586
CviAII CATG 2 cut(s) 310, 695
CviQI GTAC 6 cut(s) 76, 103, 199, 357, 523, 586
DdeI CTNAG 1 cut(s) 570
DinI GGCGCC 1 cut(s) 43
DpnI GATC 2 cut(s) 12, 537
DpnII GATC 2 cut(s) 10, 535
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Ecl136II GAGCTC 2 cut(s) 53, 542
Eco130I CCWWGG 2 cut(s) 208, 378
Eco24I GRGCYC 3 cut(s) 55, 124, 544
Eco53kI GAGCTC 2 cut(s) 53, 542
Eco57I CTGAAG 1 cut(s) 132
EcoICRI GAGCTC 2 cut(s) 53, 542
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 208, 378
EcoT38I GRGCYC 3 cut(s) 55, 124, 544
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 208, 378
FaeI CATG 2 cut(s) 313, 698
FaiI YATR 8 cut(s) 163, 258, 283, 311, 365, 488, 671, 696
FaqI GGGAC 2 cut(s) 226, 469
FatI CATG 2 cut(s) 309, 694
Fnu4HI GCNGC 4 cut(s) 339, 342, 345, 706
FokI GGATG 3 cut(s) 56, 376, 644
FriOI GRGCYC 3 cut(s) 55, 124, 544
Fsp4HI GCNGC 4 cut(s) 339, 342, 345, 706
GlaI GCGC 1 cut(s) 43
GluI GCNGC 4 cut(s) 339, 342, 345, 706
GsaI CCCAGC 1 cut(s) 678
GsuI CTGGAG 1 cut(s) 583
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 207
HapII CCGG 2 cut(s) 39, 348
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 313, 698
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HinfI GANTC 3 cut(s) 17, 432, 698
HpaII CCGG 2 cut(s) 39, 348
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 3 cut(s) 119, 154, 451
Hpy188III TCNNGA 5 cut(s) 14, 83, 436, 600, 695
Hpy8I GTNNAC 1 cut(s) 525
HpyCH4III ACNGT 4 cut(s) 266, 361, 479, 529
HpyCH4IV ACGT 2 cut(s) 35, 458
HpyCH4V TGCA 7 cut(s) 26, 93, 338, 403, 657, 688, 708
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 344, 350, 612, 621
HpyF3I CTNAG 1 cut(s) 570
HpySE526I ACGT 2 cut(s) 35, 458
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 313, 698
HspAI GCGC 1 cut(s) 42
KasI GGCGCC 1 cut(s) 41
Kzo9I GATC 2 cut(s) 10, 535
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 3 cut(s) 331, 350, 692
LweI GCATC 3 cut(s) 390, 398, 666
MaeII ACGT 2 cut(s) 35, 458
MaeIII GTNAC 1 cut(s) 367
MalI GATC 2 cut(s) 12, 537
MboI GATC 2 cut(s) 10, 535
MboII GAAGA 2 cut(s) 451, 485
MhlI GDGCHC 3 cut(s) 55, 124, 544
MluCI AATT 2 cut(s) 6, 171
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 426
MnlI CCTC 7 cut(s) 22, 90, 270, 403, 445, 460, 555
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 344, 569
MspI CCGG 2 cut(s) 39, 348
MspR9I CCNGG 2 cut(s) 203, 349
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 5 cut(s) 50, 344, 350, 612, 621
NarI GGCGCC 1 cut(s) 42
NciI CCSGG 1 cut(s) 349
NdeII GATC 2 cut(s) 10, 535
NlaIII CATG 2 cut(s) 313, 698
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 367
PagI TCATGA 1 cut(s) 694
PaqCI CACCTGC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 17, 698
PkrI GCNGC 4 cut(s) 340, 343, 346, 707
PleI GAGTC 1 cut(s) 426
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 55, 544
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 674
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 1 cut(s) 710
PstNI CAGNNNCTG 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 6 cut(s) 77, 104, 200, 358, 524, 587
RsaNI GTAC 6 cut(s) 76, 103, 199, 357, 523, 586
SacI GAGCTC 2 cut(s) 55, 544
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 4 cut(s) 339, 342, 345, 706
Sau3AI GATC 2 cut(s) 10, 535
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 2 cut(s) 203, 349
SduI GDGCHC 3 cut(s) 55, 124, 544
SfaNI GCATC 3 cut(s) 390, 398, 666
SfcI CTRYAG 1 cut(s) 706
SfoI GGCGCC 1 cut(s) 43
Sse9I AATT 2 cut(s) 6, 171
SsiI CCGC 1 cut(s) 342
SspDI GGCGCC 1 cut(s) 41
SstI GAGCTC 2 cut(s) 55, 544
StyD4I CCNGG 2 cut(s) 201, 347
StyI CCWWGG 2 cut(s) 208, 378
TaaI ACNGT 4 cut(s) 266, 361, 479, 529
TaiI ACGT 2 cut(s) 38, 461
TaqI TCGA 1 cut(s) 538
TasI AATT 2 cut(s) 6, 171
TatI WGTACW 2 cut(s) 356, 522
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 17, 698
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 338, 344, 705
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.