pycom13g28670

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001892
Physical Location & Seq
Reverse (-)
24631865 .. 24634232
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGGAGTACGAAAAACCCTTAGTCTGTGTTCTCGATGCTTCAACCTACGTGGGCTTCTGGATTCTCAAAGGGTTACTTGGGCGGGGGTTCAAAGTCCACGCAGCTATCCAGCATGAGAAGAATGGGGAAACGGAGATACAGAAGAGAATCAGGGAGACGGAGAAGGCGGAAGAGGGATTGGTAGTGTTTGATGTGGATGTTTTGGACTACCACAGCATTCTTGTGGCTTTGAAGGGCTGCACTGCTATGTTCTGCTGCCTAGATAGTCCTGATGGATATGAAGAGAAAATGGTGGATTTGGAAGTCAGAGGAGCAATTAACGTTGTGGAAGCATGTGCTCAAACAGAGAAGGTGGAGAAGGTTATCTTCACTTCTTCTTTGACTGCAGCAATATGGAGAGACAACATTTCTTATCAGAAGGATGTCGATGAGAAGTCATGGAGTGATCACCAATTTTGCAGGAAATTGAAGTTGTGGTATGCCTTGGCAAAGACGCTGTCTGAACAGGCTGCTTGGGCTTTAGCCATGGATCGAATGCTTAGGATGGTTTCCATAAATTCCGGACTTGTTCTTGGCCCTGGTGTGACACAACAAAACCCTCGCACAACTATGTCTTATCTTAACGGAGCGGCTCAAATGTATGAAAATGGGGTTCTAGCATTCGTTGATGTCAACTTTCTAGTCGATATTCATATTCGAGCTCTGGAGGACGAGTCCACATGTGGGAGGTACTTGTGCTTTAATCAAATAGTGAATACTGAGGATGAAGCTATTAAGCTTGCTCACAGCTTGAGCCCCTTGATATCATTACCACCCAGGTATGAATATCAAGGGAGTGAGGTACATACCGAGCGTTTGAGGAACGAAAAGTTGAGCAAGTTGGTTGAAGCGATGCCTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

302

Amino Acids

34.02

Weight (kDa)

5.21

Isoelectric Point (pI)

27.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 13 - 214 2.7e-09 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 13 - 233 5.3e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 48 - 130 1e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 629
AccIII TCCGGA 1 cut(s) 560
AciI CCGC 3 cut(s) 82, 167, 629
AclI AACGTT 1 cut(s) 321
AclWI GGATC 1 cut(s) 537
AcsI RAATTY 1 cut(s) 556
AfaI GTAC 3 cut(s) 8, 731, 843
AfiI CCNNNNNNNGG 1 cut(s) 723
AflIII ACRYGT 1 cut(s) 719
AgsI TTSAA 5 cut(s) 42, 91, 232, 469, 887
AjnI CCWGG 2 cut(s) 577, 815
AluBI AGCT 5 cut(s) 104, 701, 770, 778, 789
AluI AGCT 5 cut(s) 104, 701, 770, 778, 789
Alw21I GWGCWC 2 cut(s) 340, 703
Alw26I GTCTC 2 cut(s) 149, 393
AlwI GGATC 1 cut(s) 537
Aor13HI TCCGGA 1 cut(s) 560
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 5 cut(s) 101, 237, 255, 386, 509
ApoI RAATTY 1 cut(s) 556
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AseI ATTAAT 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 440
BanII GRGCYC 2 cut(s) 703, 797
Bbv12I GWGCWC 2 cut(s) 340, 703
BbvI GCAGC 5 cut(s) 113, 224, 242, 398, 496
BccI CCATC 2 cut(s) 266, 538
BciT130I CCWGG 2 cut(s) 579, 817
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 149, 393
BfaI CTAG 3 cut(s) 260, 656, 680
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 6 cut(s) 102, 238, 256, 387, 510, 630
BlsI GCNGC 6 cut(s) 103, 239, 257, 388, 511, 631
Bme1390I CCNGG 2 cut(s) 579, 817
BmgT120I GGNCC 1 cut(s) 575
BmrFI CCNGG 2 cut(s) 579, 817
BmsI GCATC 2 cut(s) 25, 882
BpmI CTGGAG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 811
BsaAI YACGTR 1 cut(s) 49
BsaJI CCNNGG 4 cut(s) 483, 525, 577, 815
BsaWI WCCGGW 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 560
BseBI CCWGG 2 cut(s) 579, 817
BseDI CCNNGG 4 cut(s) 483, 525, 577, 815
BseGI GGATG 4 cut(s) 202, 427, 549, 769
BseLI CCNNNNNNNGG 1 cut(s) 723
BseMII CTCAG 1 cut(s) 750
BseRI GAGGAG 1 cut(s) 324
BseXI GCAGC 5 cut(s) 113, 224, 242, 398, 496
BsgI GTGCAG 1 cut(s) 223
BshFI GGCC 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 340, 703
BsiSI CCGG 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 723
BsmAI GTCTC 2 cut(s) 149, 393
BsmBI CGTCTC 1 cut(s) 149
BsmI GAATGC 3 cut(s) 216, 540, 659
BsnI GGCC 1 cut(s) 576
Bsp1286I GDGCHC 3 cut(s) 340, 703, 797
Bsp13I TCCGGA 1 cut(s) 560
Bsp143I GATC 2 cut(s) 445, 529
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 3 cut(s) 82, 167, 629
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 1 cut(s) 751
BspEI TCCGGA 1 cut(s) 560
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 1 cut(s) 537
BsrBI CCGCTC 1 cut(s) 629
BssECI CCNNGG 4 cut(s) 483, 525, 577, 815
BssMI GATC 2 cut(s) 445, 529
BssT1I CCWWGG 2 cut(s) 483, 525
Bst2UI CCWGG 2 cut(s) 579, 817
Bst6I CTCTTC 3 cut(s) 137, 165, 276
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 3 cut(s) 19, 539, 759
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 4 cut(s) 202, 427, 549, 769
BstKTI GATC 2 cut(s) 448, 532
BstMAI GTCTC 2 cut(s) 149, 393
BstMBI GATC 2 cut(s) 445, 529
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNI CCWGG 2 cut(s) 579, 817
BstNSI RCATGY 2 cut(s) 336, 723
BstSCI CCNGG 2 cut(s) 577, 815
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 5 cut(s) 113, 224, 242, 398, 496
BsuRI GGCC 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 4 cut(s) 202, 427, 549, 769
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 780
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 1 cut(s) 502
Csp6I GTAC 3 cut(s) 7, 730, 842
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 5 cut(s) 113, 333, 438, 526, 720
CviQI GTAC 3 cut(s) 7, 730, 842
DdeI CTNAG 3 cut(s) 19, 539, 759
DpnI GATC 2 cut(s) 447, 531
DpnII GATC 2 cut(s) 445, 529
Eam1104I CTCTTC 3 cut(s) 137, 165, 276
EarI CTCTTC 3 cut(s) 137, 165, 276
EciI GGCGGA 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 701
Eco130I CCWWGG 2 cut(s) 483, 525
Eco24I GRGCYC 2 cut(s) 703, 797
Eco32I GATATC 1 cut(s) 804
Eco53kI GAGCTC 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 701
EcoRII CCWGG 2 cut(s) 577, 815
EcoRV GATATC 1 cut(s) 804
EcoT14I CCWWGG 2 cut(s) 483, 525
EcoT38I GRGCYC 2 cut(s) 703, 797
ErhI CCWWGG 2 cut(s) 483, 525
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 5 cut(s) 116, 336, 441, 529, 723
FalI AAGNNNNNCTT 4 cut(s) 60, 92, 350, 382
FatI CATG 5 cut(s) 112, 332, 437, 525, 719
FauI CCCGC 1 cut(s) 75
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 6 cut(s) 102, 238, 256, 387, 510, 630
FokI GGATG 4 cut(s) 209, 434, 556, 776
FriOI GRGCYC 2 cut(s) 703, 797
Fsp4HI GCNGC 6 cut(s) 102, 238, 256, 387, 510, 630
FspBI CTAG 3 cut(s) 260, 656, 680
GluI GCNGC 6 cut(s) 102, 238, 256, 387, 510, 630
GsuI CTGGAG 1 cut(s) 725
HaeIII GGCC 1 cut(s) 576
HapII CCGG 1 cut(s) 561
HgaI GACGC 1 cut(s) 502
Hin1II CATG 5 cut(s) 116, 336, 441, 529, 723
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 3 cut(s) 61, 147, 713
HpaII CCGG 1 cut(s) 561
HphI GGTGA 1 cut(s) 440
Hpy166II GTNNAC 3 cut(s) 97, 673, 717
Hpy188I TCNGA 3 cut(s) 308, 417, 502
Hpy188III TCNNGA 5 cut(s) 32, 58, 269, 561, 704
Hpy8I GTNNAC 3 cut(s) 97, 673, 717
HpyAV CCTTC 5 cut(s) 157, 226, 343, 352, 412
HpyCH4IV ACGT 2 cut(s) 48, 321
HpyCH4V TGCA 3 cut(s) 240, 386, 459
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 3 cut(s) 19, 539, 759
HpySE526I ACGT 2 cut(s) 48, 321
Hsp92II CATG 5 cut(s) 116, 336, 441, 529, 723
Kpn2I TCCGGA 1 cut(s) 560
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 2 cut(s) 445, 529
LmnI GCTCC 2 cut(s) 311, 626
Lsp1109I GCAGC 5 cut(s) 113, 224, 242, 398, 496
LweI GCATC 2 cut(s) 25, 882
MaeI CTAG 3 cut(s) 260, 656, 680
MaeII ACGT 2 cut(s) 48, 321
MaeIII GTNAC 2 cut(s) 72, 583
MalI GATC 2 cut(s) 447, 531
MbiI CCGCTC 1 cut(s) 629
MboI GATC 2 cut(s) 445, 529
MboII GAAGA 6 cut(s) 130, 154, 182, 293, 358, 366
MhlI GDGCHC 3 cut(s) 340, 703, 797
MluCI AATT 5 cut(s) 315, 452, 464, 556, 901
MlyI GAGTC 1 cut(s) 722
MnlI CCTC 8 cut(s) 166, 302, 609, 700, 720, 754, 832, 852
MroI TCCGGA 1 cut(s) 560
MseI TTAA 6 cut(s) 318, 621, 741, 774, 900, 904
MslI CAYNNNNRTG 3 cut(s) 221, 245, 608
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 2 cut(s) 579, 817
Mva1269I GAATGC 3 cut(s) 216, 540, 659
MvaI CCWGG 2 cut(s) 579, 817
MwoI GCNNNNNNNGC 1 cut(s) 515
NcoI CCATGG 1 cut(s) 525
NdeII GATC 2 cut(s) 445, 529
NlaIII CATG 5 cut(s) 116, 336, 441, 529, 723
NmuCI GTSAC 1 cut(s) 583
NspI RCATGY 2 cut(s) 336, 723
PacI TTAATTAA 1 cut(s) 904
PciI ACATGT 1 cut(s) 719
PctI GAATGC 3 cut(s) 216, 540, 659
PfeI GAWTC 2 cut(s) 61, 147
PflFI GACNNNGTC 1 cut(s) 496
PkrI GCNGC 6 cut(s) 103, 239, 257, 388, 511, 631
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 49
PscI ACATGT 1 cut(s) 719
PshBI ATTAAT 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 703
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 577, 815
PspGI CCWGG 2 cut(s) 577, 815
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 496
RsaI GTAC 3 cut(s) 8, 731, 843
RsaNI GTAC 3 cut(s) 7, 730, 842
RseI CAYNNNNRTG 3 cut(s) 221, 245, 608
SacI GAGCTC 1 cut(s) 703
SaqAI TTAA 6 cut(s) 318, 621, 741, 774, 900, 904
SatI GCNGC 6 cut(s) 102, 238, 256, 387, 510, 630
Sau3AI GATC 2 cut(s) 445, 529
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 2 cut(s) 579, 817
SduI GDGCHC 3 cut(s) 340, 703, 797
SfaNI GCATC 2 cut(s) 25, 882
SfcI CTRYAG 1 cut(s) 384
SmiMI CAYNNNNRTG 3 cut(s) 221, 245, 608
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
Sse9I AATT 5 cut(s) 315, 452, 464, 556, 901
SsiI CCGC 3 cut(s) 82, 167, 629
SspMI CTAG 3 cut(s) 260, 656, 680
SstI GAGCTC 1 cut(s) 703
StyD4I CCNGG 2 cut(s) 577, 815
StyI CCWWGG 2 cut(s) 483, 525
TaiI ACGT 2 cut(s) 51, 324
TaqI TCGA 5 cut(s) 33, 426, 532, 684, 697
TasI AATT 5 cut(s) 315, 452, 464, 556, 901
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 2 cut(s) 61, 147
Tru1I TTAA 6 cut(s) 318, 621, 741, 774, 900, 904
Tru9I TTAA 6 cut(s) 318, 621, 741, 774, 900, 904
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 5 cut(s) 101, 237, 255, 386, 509
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 5 cut(s) 294, 657, 680, 780, 837
TspGWI ACGGA 3 cut(s) 146, 173, 639
TspRI CASTG 1 cut(s) 247
Tth111I GACNNNGTC 1 cut(s) 496
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 1 cut(s) 556
XceI RCATGY 2 cut(s) 336, 723
XspI CTAG 3 cut(s) 260, 656, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.