pycom14g09640

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002094
Physical Location & Seq
Reverse (-)
11685981 .. 11693830
7850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3750 bp
ATGACACAGTATAAGAGCGCAAACTGCGACTTTCAAAAAACAAAACATATGGCAGGTTCGGGTTTAATATTCTTCAGGGCTCTCAGAACCTGGAGACACCAAATACCCTCAAGTAAAACCCAAACTTTGATTGCCCCAACAACTCTGAGATTTCGGCGTCTTCTCACCGGAAACTCCATATTCCCGCCAAATGGTGGCCAGGAGTTCGCCGGACTGGCTCCCGCCCGGATGGTCTGTCCAAACCAGATCCCAAAAGAGAGGTCGCAAAATTGTGATGATGCTATGCGCTATATCAAAATGCAAAACACTCAAGGCGAAAAGCCTCAGCCAACTGTGGTGAAAATCCCAGATCACGCAGAAGAAGTCCCTTCTCAACTTGAAGTGGACCCAATGGAATGTCCTGAATGGTTGCCTAACGGTTGGAAAGTGGAGCTGAGGACACGACAGAGTGGTGTGCAAGTTGGAAAAGAATACAAGTGTTACATTGATCCCTTCAGAGAATGTACATTTTATTCCAAGCCAGAGGTATTTCGATATCTCAAAACTGTAAAACGGAAGAACTCGAAATCTGGAACTCTAACTATAAGCAGCAAGAGATGCATAACGGAAAAAAAGGCTGTCACTACCATGCAGTTGGCAAAAAATGTTTGTTCCCTTTCATACTCTCTTTATTACACAGATCCCGACTCTGGATACATATTCCGCTCCAAAAAAGATGTTATTCGCTATCTTGAAACTGGAGAGATGAGTAAATATGCATTTAAACCCAAGGATATGTGCAATAATGATCTGAAGTTGGTTAATGATGATATTGCACCATCATCTACCACCAAAATGCAAAAACATGAGCCCCCAGTCACGAGGAGACAGCTATCACAGCCAAAAATTGAACTCTCTGACAGACGTGAAGGTAAAAGGTCATTGCTTGAAGGTGAAGGTTCTGCGATAGATCCAGGCAAAATGAGTTCTGCTCGAGATGAGCCTGTTTTGACTCCTGCTGCTTATACCTTGAATGAGAACAATTCACTTGAACCTGTGATGGAAAAAAGCAATGTGAGAAGAGCCCTAGTACACTTGGGCAAATCGAAAAACAAGGAAATGCTTAATTTGTCTCGTCGGTCCTCAAAACGACTTGGTAGGAGCAGGTTAGAACTACCAGAAACTGAAATCTCCAAGTTACTAGACAAGGGAGTATCCTCTGAAAGTGTAGTAGACTTTGCTTCAACAACTGATGTTATCCAAGAGAAGCATTTAACTGGGAACATACTGGAAAAATGTGCCAAAACTAAGGAAGATTGTTCTCCAGACACTTCAATGGAAAAAAGCAATGTCAGAAGAACCCCAATAGACTCGAGCAAATCAAAAAACAAGGAAATGCTTAATTTGTGCCGTCGCTTCTCAAAACGACTTGCTGGGAGCAGGTTGGAACTACCAGAAACTGAAAGCTTCAAGTTGCTAGACAATAGGGTACCCTCTGAAAATGGACTGGCCTTTGCTTCGACAGCTGGGATTGTCCAACAGAAAAATTTAACTGGGAACTTGCTTGAAAAATGTGCCAAAACTAAGGAAGATTGTTCCCCAAACACTGTGATGGAAAAAAGCAATGTCAGAAGAACCCTGGTAGACTCGAGCAAATCAAAAAACAAGGAAAAGCTTAATTTGTCTCGCCGGTTTTCAAAACGACTTGCTGGGAGCAGGTTGGAATTACCGGAAACTGAAATTTCGGAGTTGCCAGACAAAATGGTACCCTTAGTAAGTGGAGTTGCCTTTACTATGACAGCTGATGTTGTTCAAGAGAAGCATTTAACTGATAACATGCCGGAAAAATGTGCCAAAACTAAGGAAGATGGTTCTCCAAGCGGGTTATCACCCCCAAAAGCTGAACTCTCGGAGAGACAGGAAGGAGAAAGGTCATTGCTTGAAGTTGTAGGTTCTGTGATAGACCCAGGAAAAATGATGTGTGCTGGAAATGAGCCCGTTTTGACTCCTGCTTCTTACACCCTGAATGAGAATAATTCGCATAAGACTGTTGAGAAAAAAAGCAACGTCAGAAAAGCTCCAATAGGCTCGAGCAAATCAAAAAACAAGGAAAAACTTATTTTGTCTCGTCGGTCCTCAAAACGACTTGCTGGGCTTGAACCAGAGGAGGTTGCTAACTTGGCTTCCAGTGAACGAGCTGTTCAAGCTCCAACTAGGAAACCTAGTAAAAGTGATACCAGTCAAGACGTAGGTTTGGCTTCAGATCTTGTAAACAGAGCATCTCAGCAGCTTGGGGCTGCATCAGAAGCAGAGGTTTCACATCATGCTTTAACTGGCATAAAATCTATATCTCATGAGGACAAGATGCCAATTGATGACCCAGTGGTTCCCAGAGCGCAACAAGAGCTGGAAACTGAGAAAATGGATGCTGAGAAGCCAGAGCCAGAGCTCAGCTTTCTGTTTGGTTCAGACCCATGCTTAGAATTCGCATTCAAAACACTTACAGGGGAATTGCCAATAGCTGATACTGTGTATAACAGATCTATTTTGAGACCTGCAGCTGATATGCTGCATAAAAAAAATTTAGTTGACAGTGGGATGGAAAAGAACTGCAGTAGAAACAAATCCAAGAAAAATAAAGATCTTAAGTTGTCCAGTTGGTCTTCAAAACAGTATGCTGGGGTTCAACCTGAGCTGCTGGCTAATACTACATCCAAGGAACGAGCTATCCGAAATGCAACTGAAAATTCATGTCAAAGTATAGCCATCCCGGCTGTGGATTTGGCAGATGAAGCATCTCAACCGCTTGAGATTACACCAGTGGTGAAGCTTGCCAGTCGTGCTTGTACTGCTATATACAATTCAATGCAAGAGGAGACGTTGTACAAGAGTGCGAAGGTTCTTGAAGAGCATGCTGTTCCTCAGGAGAAACCCCAGAAGTTTGAAACCGAGAAGGCATATGTTGAGAATCCAGAGCCACAGTTCCCTTTCCCTTTCACGGATTCATGGTCTGACCCATGCCTCGATTTTGCATTCAAGACTCTTACAGGTGCAATTCCATTAGAGGATGATTATTATCAAGGTTACTTCCAAGAAAAACTTGACACATCTCACAATATGGCACTATCAGATTTTGGCTCACCTAGCTTGTTTCGAAGTGATAATTTACCTCAGTTCGATCTGCCAGAACAATCTGCTTCTGGCCAACAGTTATCCATGAATTCTGCATTCCTGCCTTCTGGAAATGTGAGCATGCCGAATTGCAGTGGAATTGGTTCAGGCCAACAGTTATCTAGGAATCCTTCATCCCTCCCAGCAGGAAATGTGAGCATTTCAAATTGCAGTGGGGTTTGTTCAGGCCAACAGTTGTCCATGAATTCTGCATTCCTGCCTTCAGGAAATGTGAGCATGCCGAATTGCGGTGGAATTGGTTCAGGTCAACAGTCATCTAGGAATCCTTCATCCCTCCCTCCAGGAAACGTGAGCATGTCAAATTGCAGCGGGGTTTGTTCAGGCCAACAGTTATTTAGGAATCCTACATCCCTCCCTACAGGAAACTTGAGCATTTCAAATTGCAGTGGGGTTTGTTCAGGCCACCAGTTATCCATGAATTCTTCATTCCAGCCCGCTGGAAACATGAGCACATCAAATTTCAGCGCGGTGTGTTCAGGCCAACAGTTATCTCTAAATTCTTCATTACTGCCTGCCGGAAGCTTGGTTGGTTCGCAGTGGCCTTGTGTAGATGATTATCATGGAAAGGTGAAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

1250

Amino Acids

137.39

Weight (kDa)

8.73

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 132 - 184 4.4e-06 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 44, 1134, 1410, 1686, 2542
Acc65I GGTACC 2 cut(s) 1468, 1744
AccB1I GGYRCC 2 cut(s) 1468, 1744
AccB7I CCANNNNNTGG 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 705
AccI GTMKAC 2 cut(s) 1212, 1623
AccII CGCG 1 cut(s) 3638
AciI CCGC 9 cut(s) 185, 222, 703, 1860, 2783, 3399, 3480, 3606, 3638
AclWI GGATC 4 cut(s) 241, 482, 674, 944
AcoI YGGCCR 2 cut(s) 196, 3181
AcuI CTGAAG 5 cut(s) 58, 478, 812, 2223, 3357
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 6 cut(s) 505, 1071, 1470, 1746, 2827, 2864
AfiI CCNNNNNNNGG 6 cut(s) 191, 194, 689, 2755, 2977, 3398
AflII CTTAAG 1 cut(s) 2624
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 6 cut(s) 89, 198, 952, 1617, 1945, 3451
AloI GAACNNNNNNTCC 2 cut(s) 2691, 2723
Alw21I GWGCWC 2 cut(s) 2430, 3623
Alw26I GTCTC 8 cut(s) 88, 859, 1116, 1668, 1888, 2109, 2524, 2849
AlwI GGATC 4 cut(s) 241, 482, 674, 944
AlwNI CAGNNNCTG 3 cut(s) 90, 1163, 1439
Ama87I CYCGRG 4 cut(s) 972, 1351, 1627, 2068
AoxI GGCC 9 cut(s) 196, 1488, 3181, 3259, 3337, 3493, 3571, 3649, 3710
ApeKI GCWGC 8 cut(s) 588, 998, 2266, 2276, 2537, 2548, 2674, 3477
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 2 cut(s) 3253, 3409
Asp718I GGTACC 2 cut(s) 1468, 1744
AspLEI GCGC 4 cut(s) 20, 288, 2377, 3638
AspS9I GGNCC 3 cut(s) 385, 1119, 2112
AsuC2I CCSGG 2 cut(s) 226, 2750
AsuHPI GGTGA 6 cut(s) 157, 349, 944, 1860, 2815, 3111
AsuII TTCGAA 1 cut(s) 3133
AvaI CYCGRG 4 cut(s) 972, 1351, 1627, 2068
AvaII GGWCC 3 cut(s) 385, 1119, 2112
AxyI CCTNAGG 1 cut(s) 2901
BalI TGGCCA 2 cut(s) 198, 3183
BanI GGYRCC 2 cut(s) 1468, 1744
BanII GRGCYC 5 cut(s) 82, 852, 1066, 1977, 2430
BauI CACGAG 1 cut(s) 859
BbsI GAAGAC 2 cut(s) 152, 2634
Bbv12I GWGCWC 2 cut(s) 2430, 3623
BbvCI CCTCAGC 2 cut(s) 324, 434
BbvI GCAGC 8 cut(s) 600, 985, 2263, 2278, 2535, 2549, 2661, 3489
BccI CCATC 7 cut(s) 223, 826, 1033, 1585, 1841, 2572, 2753
BceAI ACGGC 1 cut(s) 1374
BciT130I CCWGG 6 cut(s) 91, 200, 954, 1619, 1947, 3453
BciVI GTATCC 2 cut(s) 686, 1204
BcnI CCSGG 2 cut(s) 226, 2750
BcoDI GTCTC 8 cut(s) 88, 859, 1116, 1668, 1888, 2109, 2524, 2849
BfaI CTAG 8 cut(s) 1067, 1181, 1457, 2193, 2202, 3123, 3273, 3429
BfmI CTRYAG 3 cut(s) 2535, 2590, 3528
BfrI CTTAAG 1 cut(s) 2624
BfuAI ACCTGC 5 cut(s) 44, 1134, 1410, 1686, 2542
BfuI GTATCC 2 cut(s) 686, 1204
BglI GCCNNNNNGGC 2 cut(s) 215, 2750
BglII AGATCT 3 cut(s) 2242, 2519, 2620
BisI GCNGC 8 cut(s) 589, 999, 2267, 2277, 2538, 2549, 2675, 3478
BlpI GCTNAGC 1 cut(s) 2429
BlsI GCNGC 8 cut(s) 590, 1000, 2268, 2278, 2539, 2550, 2676, 3479
Bme1390I CCNGG 8 cut(s) 91, 200, 226, 954, 1619, 1947, 2750, 3453
Bme18I GGWCC 3 cut(s) 385, 1119, 2112
BmeT110I CYCGRG 4 cut(s) 972, 1351, 1627, 2068
BmgBI CACGTC 1 cut(s) 905
BmgT120I GGNCC 3 cut(s) 385, 1119, 2112
BmiI GGNNCC 5 cut(s) 219, 387, 1470, 1746, 2367
BmrFI CCNGG 8 cut(s) 91, 200, 226, 954, 1619, 1947, 2750, 3453
BmrI ACTGGG 4 cut(s) 848, 1266, 1542, 2354
BmsI GCATC 7 cut(s) 268, 587, 2267, 2288, 2334, 2395, 2783
BmuI ACTGGG 4 cut(s) 848, 1266, 1542, 2354
BpiI GAAGAC 2 cut(s) 152, 2634
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 4 cut(s) 112, 759, 1287, 3435
Bpu10I CCTNAGC 3 cut(s) 324, 434, 2670
Bpu1102I GCTNAGC 1 cut(s) 2429
Bpu14I TTCGAA 1 cut(s) 3133
BpuEI CTTGAG 4 cut(s) 94, 294, 2807, 3559
BpuMI CCSGG 2 cut(s) 226, 2750
BsaBI GATNNNNATC 2 cut(s) 3054, 3726
BsaHI GRCGYC 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 2524
BsaJI CCNNGG 4 cut(s) 768, 1617, 1945, 2694
BsaWI WCCGGW 2 cut(s) 167, 1708
BsaXI ACNNNNNCTCC 2 cut(s) 2846, 2876
Bsc4I CCNNNNNNNGG 6 cut(s) 191, 194, 689, 2755, 2977, 3398
Bse118I RCCGGY 1 cut(s) 1668
Bse21I CCTNAGG 1 cut(s) 2901
Bse3DI GCAATG 5 cut(s) 920, 1057, 1333, 1609, 1913
Bse8I GATNNNNATC 2 cut(s) 3054, 3726
BseBI CCWGG 6 cut(s) 91, 200, 954, 1619, 1947, 3453
BseDI CCNNGG 4 cut(s) 768, 1617, 1945, 2694
BseGI GGATG 9 cut(s) 234, 2410, 2583, 2690, 2745, 3052, 3284, 3440, 3518
BseJI GATNNNNATC 2 cut(s) 3054, 3726
BseLI CCNNNNNNNGG 6 cut(s) 191, 194, 689, 2755, 2977, 3398
BseMI GCAATG 5 cut(s) 920, 1057, 1333, 1609, 1913
BseRI GAGGAG 3 cut(s) 877, 2159, 2867
BseXI GCAGC 8 cut(s) 600, 985, 2263, 2278, 2535, 2549, 2661, 3489
BseYI CCCAGC 6 cut(s) 1412, 1505, 1688, 2129, 2657, 3292
Bsh1236I CGCG 1 cut(s) 3638
BshFI GGCC 9 cut(s) 198, 1490, 3183, 3261, 3339, 3495, 3573, 3651, 3712
BshNI GGYRCC 2 cut(s) 1468, 1744
BsiHKAI GWGCWC 2 cut(s) 2430, 3623
BsiHKCI CYCGRG 4 cut(s) 972, 1351, 1627, 2068
BsiSI CCGG 8 cut(s) 168, 210, 226, 1669, 1709, 1820, 2750, 3687
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 6 cut(s) 191, 194, 689, 2755, 2977, 3398
BsmAI GTCTC 8 cut(s) 88, 859, 1116, 1668, 1888, 2109, 2524, 2849
BsmBI CGTCTC 1 cut(s) 2849
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 4 cut(s) 2468, 3011, 3206, 3362
BsnI GGCC 9 cut(s) 198, 1490, 3183, 3261, 3339, 3495, 3573, 3651, 3712
Bso31I GGTCTC 1 cut(s) 2524
BsoBI CYCGRG 4 cut(s) 972, 1351, 1627, 2068
Bsp119I TTCGAA 1 cut(s) 3133
Bsp1286I GDGCHC 6 cut(s) 82, 852, 1066, 1977, 2430, 3623
Bsp1407I TGTACA 2 cut(s) 503, 2862
Bsp1720I GCTNAGC 1 cut(s) 2429
BspACI CCGC 9 cut(s) 185, 222, 703, 1860, 2783, 3399, 3480, 3606, 3638
BspANI GGCC 9 cut(s) 198, 1490, 3183, 3261, 3339, 3495, 3573, 3651, 3712
BspFNI CGCG 1 cut(s) 3638
BspHI TCATGA 1 cut(s) 2332
BspLI GGNNCC 5 cut(s) 219, 387, 1470, 1746, 2367
BspMAI CTGCAG 2 cut(s) 2539, 2594
BspMI ACCTGC 5 cut(s) 44, 1134, 1410, 1686, 2542
BspPI GGATC 4 cut(s) 241, 482, 674, 944
BspQI GCTCTTC 2 cut(s) 1054, 2880
BspT104I TTCGAA 1 cut(s) 3133
BspT107I GGYRCC 2 cut(s) 1468, 1744
BspTI CTTAAG 1 cut(s) 2624
BspTNI GGTCTC 1 cut(s) 2524
BsrBI CCGCTC 1 cut(s) 705
BsrDI GCAATG 5 cut(s) 920, 1057, 1333, 1609, 1913
BsrFI RCCGGY 1 cut(s) 1668
BsrGI TGTACA 2 cut(s) 503, 2862
BssAI RCCGGY 1 cut(s) 1668
BssECI CCNNGG 4 cut(s) 768, 1617, 1945, 2694
BssNI GRCGYC 1 cut(s) 157
BssSI CACGAG 1 cut(s) 859
BssT1I CCWWGG 2 cut(s) 768, 2694
Bst2BI CACGAG 1 cut(s) 859
Bst2UI CCWGG 6 cut(s) 91, 200, 954, 1619, 1947, 3453
Bst6I CTCTTC 2 cut(s) 1054, 2880
BstACI GRCGYC 1 cut(s) 157
BstAFI CTTAAG 1 cut(s) 2624
BstAPI GCANNNNNTGC 1 cut(s) 597
BstAUI TGTACA 2 cut(s) 503, 2862
BstBI TTCGAA 1 cut(s) 3133
BstC8I GCNNGC 7 cut(s) 2679, 2811, 2892, 3233, 3389, 3606, 3684
BstF5I GGATG 9 cut(s) 234, 2410, 2583, 2690, 2745, 3052, 3284, 3440, 3518
BstFNI CGCG 1 cut(s) 3638
BstHHI GCGC 4 cut(s) 20, 288, 2377, 3638
BstMAI GTCTC 8 cut(s) 88, 859, 1116, 1668, 1888, 2109, 2524, 2849
BstNI CCWGG 6 cut(s) 91, 200, 954, 1619, 1947, 3453
BstNSI RCATGY 5 cut(s) 1819, 2894, 3235, 3391, 3469
BstSCI CCNGG 8 cut(s) 89, 198, 224, 952, 1617, 1945, 2748, 3451
BstSFI CTRYAG 3 cut(s) 2535, 2590, 3528
BstUI CGCG 1 cut(s) 3638
BstV1I GCAGC 8 cut(s) 600, 985, 2263, 2278, 2535, 2549, 2661, 3489
BstV2I GAAGAC 2 cut(s) 152, 2634
BstX2I RGATCY 6 cut(s) 246, 679, 949, 2242, 2519, 2620
BstXI CCANNNNNNTGG 2 cut(s) 634, 3608
BstYI RGATCY 6 cut(s) 246, 679, 949, 2242, 2519, 2620
Bsu36I CCTNAGG 1 cut(s) 2901
BsuI GTATCC 2 cut(s) 686, 1204
BsuRI GGCC 9 cut(s) 198, 1490, 3183, 3261, 3339, 3495, 3573, 3651, 3712
BtrI CACGTC 1 cut(s) 905
BtsCI GGATG 9 cut(s) 234, 2410, 2583, 2690, 2745, 3052, 3284, 3440, 3518
BtsI GCAGTG 4 cut(s) 3250, 3328, 3562, 3713
BtsIMutI CAGTG 9 cut(s) 1584, 2173, 2367, 2578, 2805, 3250, 3328, 3562, 3713
BveI ACCTGC 5 cut(s) 44, 1134, 1410, 1686, 2542
Cac8I GCNNGC 7 cut(s) 2679, 2811, 2892, 3233, 3389, 3606, 3684
CaiI CAGNNNCTG 3 cut(s) 90, 1163, 1439
CciI TCATGA 1 cut(s) 2332
CfoI GCGC 4 cut(s) 20, 288, 2377, 3638
Cfr10I RCCGGY 1 cut(s) 1668
Cfr13I GGNCC 3 cut(s) 385, 1119, 2112
CseI GACGC 1 cut(s) 146
Csp6I GTAC 6 cut(s) 504, 1070, 1469, 1745, 2826, 2863
CviQI GTAC 6 cut(s) 504, 1070, 1469, 1745, 2826, 2863
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 2 cut(s) 196, 3181
Eam1104I CTCTTC 2 cut(s) 1054, 2880
EarI CTCTTC 2 cut(s) 1054, 2880
Ecl136II GAGCTC 1 cut(s) 2428
Eco130I CCWWGG 2 cut(s) 768, 2694
Eco24I GRGCYC 5 cut(s) 82, 852, 1066, 1977, 2430
Eco31I GGTCTC 1 cut(s) 2524
Eco32I GATATC 1 cut(s) 536
Eco47I GGWCC 3 cut(s) 385, 1119, 2112
Eco53kI GAGCTC 1 cut(s) 2428
Eco57I CTGAAG 5 cut(s) 58, 478, 812, 2223, 3357
Eco81I CCTNAGG 1 cut(s) 2901
Eco88I CYCGRG 4 cut(s) 972, 1351, 1627, 2068
EcoICRI GAGCTC 1 cut(s) 2428
EcoRI GAATTC 4 cut(s) 2462, 3199, 3355, 3589
EcoRII CCWGG 6 cut(s) 89, 198, 952, 1617, 1945, 3451
EcoRV GATATC 1 cut(s) 536
EcoT14I CCWWGG 2 cut(s) 768, 2694
EcoT22I ATGCAT 2 cut(s) 602, 760
EcoT38I GRGCYC 5 cut(s) 82, 852, 1066, 1977, 2430
ErhI CCWWGG 2 cut(s) 768, 2694
Esp3I CGTCTC 1 cut(s) 2849
FalI AAGNNNNNCTT 2 cut(s) 3063, 3095
FaqI GGGAC 1 cut(s) 350
FauI CCCGC 5 cut(s) 192, 229, 1853, 3473, 3613
FauNDI CATATG 2 cut(s) 48, 2938
FblI GTMKAC 2 cut(s) 1212, 1623
Fnu4HI GCNGC 8 cut(s) 589, 999, 2267, 2277, 2538, 2549, 2675, 3478
FokI GGATG 9 cut(s) 241, 2417, 2590, 2677, 2732, 3059, 3271, 3427, 3505
FriOI GRGCYC 5 cut(s) 82, 852, 1066, 1977, 2430
Fsp4HI GCNGC 8 cut(s) 589, 999, 2267, 2277, 2538, 2549, 2675, 3478
FspBI CTAG 8 cut(s) 1067, 1181, 1457, 2193, 2202, 3123, 3273, 3429
GlaI GCGC 4 cut(s) 19, 287, 2376, 3637
GluI GCNGC 8 cut(s) 589, 999, 2267, 2277, 2538, 2549, 2675, 3478
GsaI CCCAGC 6 cut(s) 1416, 1509, 1692, 2133, 2661, 3296
GsuI CTGGAG 4 cut(s) 112, 759, 1287, 3435
HaeIII GGCC 9 cut(s) 198, 1490, 3183, 3261, 3339, 3495, 3573, 3651, 3712
HapII CCGG 8 cut(s) 168, 210, 226, 1669, 1709, 1820, 2750, 3687
HgaI GACGC 1 cut(s) 146
HhaI GCGC 4 cut(s) 20, 288, 2377, 3638
Hin1I GRCGYC 1 cut(s) 157
Hin6I GCGC 4 cut(s) 18, 286, 2375, 3636
HinP1I GCGC 4 cut(s) 18, 286, 2375, 3636
HincII GTYRAC 2 cut(s) 2569, 3419
HindII GTYRAC 2 cut(s) 2569, 3419
HindIII AAGCTT 4 cut(s) 1444, 1652, 2807, 3691
HpaII CCGG 8 cut(s) 168, 210, 226, 1669, 1709, 1820, 2750, 3687
HphI GGTGA 6 cut(s) 157, 349, 944, 1860, 2815, 3111
Hpy166II GTNNAC 8 cut(s) 385, 1072, 1213, 1624, 2171, 2251, 2569, 3419
Hpy8I GTNNAC 8 cut(s) 385, 1072, 1213, 1624, 2171, 2251, 2569, 3419
Hpy99I CGWCG 3 cut(s) 1119, 1395, 2112
HpyCH4IV ACGT 5 cut(s) 904, 2046, 2226, 2858, 3459
HpySE526I ACGT 5 cut(s) 904, 2046, 2226, 2858, 3459
Hsp92I GRCGYC 1 cut(s) 157
HspAI GCGC 4 cut(s) 18, 286, 2375, 3636
KpnI GGTACC 2 cut(s) 1472, 1748
LguI GCTCTTC 2 cut(s) 1054, 2880
LmnI GCTCC 8 cut(s) 223, 430, 710, 1140, 1416, 1692, 2062, 2191
Lsp1109I GCAGC 8 cut(s) 600, 985, 2263, 2278, 2535, 2549, 2661, 3489
LweI GCATC 7 cut(s) 268, 587, 2267, 2288, 2334, 2395, 2783
MaeI CTAG 8 cut(s) 1067, 1181, 1457, 2193, 2202, 3123, 3273, 3429
MaeII ACGT 5 cut(s) 904, 2046, 2226, 2858, 3459
MaeIII GTNAC 5 cut(s) 479, 619, 856, 1176, 3062
MbiI CCGCTC 1 cut(s) 705
MfeI CAATTG 1 cut(s) 2349
MflI RGATCY 6 cut(s) 246, 679, 949, 2242, 2519, 2620
MhlI GDGCHC 6 cut(s) 82, 852, 1066, 1977, 2430, 3623
MlsI TGGCCA 2 cut(s) 198, 3183
MluNI TGGCCA 2 cut(s) 198, 3183
MlyI GAGTC 6 cut(s) 680, 985, 1343, 1619, 1978, 3013
MmeI TCCRAC 6 cut(s) 401, 442, 1404, 1540, 1680, 2213
Mox20I TGGCCA 2 cut(s) 198, 3183
Mph1103I ATGCAT 2 cut(s) 602, 760
MroXI GAANNNNTTC 2 cut(s) 3253, 3409
MscI TGGCCA 2 cut(s) 198, 3183
MslI CAYNNNNRTG 4 cut(s) 626, 833, 1313, 1589
Msp20I TGGCCA 2 cut(s) 198, 3183
MspA1I CMGCKG 5 cut(s) 1505, 1781, 2540, 3480, 3608
MspCI CTTAAG 1 cut(s) 2624
MspI CCGG 8 cut(s) 168, 210, 226, 1669, 1709, 1820, 2750, 3687
MspR9I CCNGG 8 cut(s) 91, 200, 226, 954, 1619, 1947, 2750, 3453
MunI CAATTG 1 cut(s) 2349
Mva1269I GAATGC 4 cut(s) 2468, 3011, 3206, 3362
MvaI CCWGG 6 cut(s) 91, 200, 954, 1619, 1947, 3453
MvnI CGCG 1 cut(s) 3638
NciI CCSGG 2 cut(s) 226, 2750
NdeI CATATG 2 cut(s) 48, 2938
NlaIV GGNNCC 5 cut(s) 219, 387, 1470, 1746, 2367
NmuCI GTSAC 2 cut(s) 619, 856
NsiI ATGCAT 2 cut(s) 602, 760
NspI RCATGY 5 cut(s) 1819, 2894, 3235, 3391, 3469
NspV TTCGAA 1 cut(s) 3133
PaeI GCATGC 3 cut(s) 2894, 3235, 3391
PaeR7I CTCGAG 4 cut(s) 972, 1351, 1627, 2068
PagI TCATGA 1 cut(s) 2332
PciSI GCTCTTC 2 cut(s) 1054, 2880
PcsI WCGNNNNNNNCGW 1 cut(s) 2707
PctI GAATGC 4 cut(s) 2468, 3011, 3206, 3362
PdmI GAANNNNTTC 2 cut(s) 3253, 3409
PfeI GAWTC 5 cut(s) 2947, 2981, 3277, 3433, 3511
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 3451
PkrI GCNGC 8 cut(s) 590, 1000, 2268, 2278, 2539, 2550, 2676, 3479
PleI GAGTC 6 cut(s) 680, 985, 1343, 1619, 1978, 3013
PpsI GAGTC 6 cut(s) 680, 985, 1343, 1619, 1978, 3013
Psp124BI GAGCTC 1 cut(s) 2430
Psp6I CCWGG 6 cut(s) 89, 198, 952, 1617, 1945, 3451
PspFI CCCAGC 6 cut(s) 1412, 1505, 1688, 2129, 2657, 3292
PspGI CCWGG 6 cut(s) 89, 198, 952, 1617, 1945, 3451
PspN4I GGNNCC 5 cut(s) 219, 387, 1470, 1746, 2367
PspPI GGNCC 3 cut(s) 385, 1119, 2112
PspXI VCTCGAGB 3 cut(s) 1351, 1627, 2068
PstI CTGCAG 2 cut(s) 2539, 2594
PstNI CAGNNNCTG 3 cut(s) 90, 1163, 1439
PsuI RGATCY 6 cut(s) 246, 679, 949, 2242, 2519, 2620
PvuII CAGCTG 3 cut(s) 1505, 1781, 2540
RsaI GTAC 6 cut(s) 505, 1071, 1470, 1746, 2827, 2864
RsaNI GTAC 6 cut(s) 504, 1070, 1469, 1745, 2826, 2863
RseI CAYNNNNRTG 4 cut(s) 626, 833, 1313, 1589
SacI GAGCTC 1 cut(s) 2430
SapI GCTCTTC 2 cut(s) 1054, 2880
SatI GCNGC 8 cut(s) 589, 999, 2267, 2277, 2538, 2549, 2675, 3478
Sau96I GGNCC 3 cut(s) 385, 1119, 2112
SchI GAGTC 6 cut(s) 680, 985, 1343, 1619, 1978, 3013
ScrFI CCNGG 8 cut(s) 91, 200, 226, 954, 1619, 1947, 2750, 3453
SduI GDGCHC 6 cut(s) 82, 852, 1066, 1977, 2430, 3623
SfaNI GCATC 7 cut(s) 268, 587, 2267, 2288, 2334, 2395, 2783
SfcI CTRYAG 3 cut(s) 2535, 2590, 3528
Sfr274I CTCGAG 4 cut(s) 972, 1351, 1627, 2068
SfuI TTCGAA 1 cut(s) 3133
SinI GGWCC 3 cut(s) 385, 1119, 2112
SlaI CTCGAG 4 cut(s) 972, 1351, 1627, 2068
SmiMI CAYNNNNRTG 4 cut(s) 626, 833, 1313, 1589
SmlI CTYRAG 9 cut(s) 109, 309, 972, 1351, 1627, 2068, 2624, 2786, 3538
SmoI CTYRAG 9 cut(s) 109, 309, 972, 1351, 1627, 2068, 2624, 2786, 3538
SphI GCATGC 3 cut(s) 2894, 3235, 3391
SsiI CCGC 9 cut(s) 185, 222, 703, 1860, 2783, 3399, 3480, 3606, 3638
SspI AATATT 1 cut(s) 69
SspMI CTAG 8 cut(s) 1067, 1181, 1457, 2193, 2202, 3123, 3273, 3429
SstI GAGCTC 1 cut(s) 2430
StyD4I CCNGG 8 cut(s) 89, 198, 224, 952, 1617, 1945, 2748, 3451
StyI CCWWGG 2 cut(s) 768, 2694
TaiI ACGT 5 cut(s) 907, 2049, 2229, 2861, 3462
TaqII GACCGA 2 cut(s) 1107, 2100
TatI WGTACW 4 cut(s) 503, 1069, 2825, 2862
TfiI GAWTC 5 cut(s) 2947, 2981, 3277, 3433, 3511
TscAI CASTG 9 cut(s) 1591, 2173, 2367, 2578, 2805, 3250, 3328, 3562, 3713
TseFI GTSAC 2 cut(s) 619, 856
TseI GCWGC 8 cut(s) 588, 998, 2266, 2276, 2537, 2548, 2674, 3477
Tsp45I GTSAC 2 cut(s) 619, 856
TspGWI ACGGA 3 cut(s) 568, 620, 2993
TspRI CASTG 9 cut(s) 1591, 2173, 2367, 2578, 2805, 3250, 3328, 3562, 3713
Van91I CCANNNNNTGG 1 cut(s) 194
Vha464I CTTAAG 1 cut(s) 2624
VpaK11BI GGWCC 3 cut(s) 385, 1119, 2112
XceI RCATGY 5 cut(s) 1819, 2894, 3235, 3391, 3469
XcmI CCANNNNNNNNNTGG 1 cut(s) 2752
XhoI CTCGAG 4 cut(s) 972, 1351, 1627, 2068
XmiI GTMKAC 2 cut(s) 1212, 1623
XmnI GAANNNNTTC 2 cut(s) 3253, 3409
XspI CTAG 8 cut(s) 1067, 1181, 1457, 2193, 2202, 3123, 3273, 3429
Zsp2I ATGCAT 2 cut(s) 602, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.