pycom15g14190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003311
Physical Location & Seq
Forward (+)
9662529 .. 9664058
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1308 bp
ATGGAAAAGATTTGGAAAGAATCCCTTTCCTGTCGGCCCGGTCAATACGTGTCGATAAGACCAACCACTCCACCCATTCATGTTTTGACACGTGGTTTTCAGTCCGCTTCACGGCAACTAAAGCGTCGAGCCACACATCATTACTCCTCTCTTACCCCAAAATTACCAGTGGAAAACCAAGGCAATACCGTAATTCAACCGCGGATGGAACTGCAAAACCAAGTCGGTCAAAATCTTAACCCCGCCCCACGCGCCTCAGACGGTTCCAAACCTCACGCGACGTCACGTGAGTTACCGGAACCTCTCTATAAATACACGCTCACGCACGCTTTGCAAAATATAGCGTTTCTTCTCAGACAACAAAATTTTGAGAAAATTAGAAATTTAATCGATTTTTCTTTTGTTTTCGCAATTTTTAATTTTGTTCTTCTCTTTCTTCTTCCGAGGGAAAAAAATGAGGACGGCGATTCTCCGATCGATCAGGATTGCCTCCGAGGTCGCTTCCGCCACGAAGCCCTAACCCTAACCCTCTCCCCCCGCTCCGGATCACGAAGGAGCTCTAATTTCTCTAACCCTAACTCTAATTCCACCCCCAACCATGGCTCCACCGCCAATATTCATCAATCTCGCCGCCGGAAGAGGAGTCCGATGGGGCTTCAGTCTAATCAACACGAACGACCTCGCGATCGGTGCTCTGATCGGGCCGCGGTTGCGAAGGCACCGGGGAAAAACCTGGTGATTGGACAGGTCAAGATCCTGAGACGCGGCGAGGATTTGAACCCGGAGAAGAACAACCGAGGTCTCGGAGTTGTTGCCCGCGATGACCGCAAGCCGAAGGCGAAGAAGGAAAACGATCCGGATTTGATTTTGGGATCCACGGACCGTTTGGGACCCGACCCGGAGACCGTGCAGAAGCAGATTAAGGCTGCGGAGTTCAAAGTTATGGATGCGATTTACGCCGGATCGAGCGCTTTCTACGCGTCTCCGCCTCCAAGCTCGGTGCCACTGCCTAGTTTTTTGGGAAGGAACGGCACCGCCACCAGCGATCTCCGCCGGCTGCTGCGACTCGATTTGTTCCGGCTTTGCGATTTGGCAGCCGTGATTGAATCGTTTGATCGAAATCGATTAGTTGGTAATGGTTGTTCGTTTAGTCCCAATTTGCTTCCTTTATTTTTTCAAGAGGAAAGTCGATTGAGGTTTCTGCAAGTTGAAGGAAGAAGATTAGTAGGAAAGTTTGGGTGTTTGGCGCCTTCTATATTTTACTTGGTATGCAAGAAATGTTTACGAGGTTGGAAGAAAGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNRC

Protein Analysis

436

Amino Acids

49.06

Weight (kDa)

10.16

Isoelectric Point (pI)

60.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
AccB1I GGYRCC 4 cut(s) 718, 1000, 1031, 1246
AccBSI CCGCTC 1 cut(s) 540
AccII CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
AccIII TCCGGA 2 cut(s) 542, 856
AclWI GGATC 6 cut(s) 553, 748, 848, 867, 880, 970
AcsI RAATTY 2 cut(s) 364, 382
AcuI CTGAAG 1 cut(s) 641
AcvI CACGTG 2 cut(s) 92, 287
AcyI GRCGYC 2 cut(s) 281, 1247
AfeI AGCGCT 1 cut(s) 970
AfiI CCNNNNNNNGG 3 cut(s) 111, 542, 599
AflIII ACRYGT 3 cut(s) 48, 89, 978
AgsI TTSAA 6 cut(s) 197, 778, 937, 1106, 1178, 1211
AjnI CCWGG 1 cut(s) 732
AluBI AGCT 3 cut(s) 558, 996, 1304
AluI AGCT 3 cut(s) 558, 996, 1304
Alw21I GWGCWC 2 cut(s) 560, 695
Alw26I GTCTC 4 cut(s) 754, 806, 896, 987
AlwI GGATC 6 cut(s) 553, 748, 848, 867, 880, 970
Aor13HI TCCGGA 2 cut(s) 542, 856
Aor51HI AGCGCT 1 cut(s) 970
AoxI GGCC 2 cut(s) 35, 702
ApeKI GCWGC 4 cut(s) 926, 1057, 1060, 1094
ApoI RAATTY 2 cut(s) 364, 382
AspLEI GCGC 3 cut(s) 254, 971, 1249
AspS9I GGNCC 4 cut(s) 36, 702, 880, 890
AsuC2I CCSGG 4 cut(s) 39, 723, 782, 899
AsuHPI GGTGA 1 cut(s) 748
AvaII GGWCC 2 cut(s) 880, 890
BamHI GGATCC 1 cut(s) 872
BanI GGYRCC 4 cut(s) 718, 1000, 1031, 1246
BanII GRGCYC 1 cut(s) 560
BbrPI CACGTG 2 cut(s) 92, 287
Bbv12I GWGCWC 2 cut(s) 560, 695
BbvI GCAGC 4 cut(s) 913, 1044, 1047, 1106
BccI CCATC 2 cut(s) 199, 643
BceAI ACGGC 4 cut(s) 128, 478, 1045, 1082
BciT130I CCWGG 1 cut(s) 734
BcnI CCSGG 4 cut(s) 39, 723, 782, 899
BcoDI GTCTC 4 cut(s) 754, 806, 896, 987
BfaI CTAG 1 cut(s) 1011
BfoI RGCGCY 2 cut(s) 972, 1250
BisI GCNGC 7 cut(s) 631, 705, 766, 927, 1058, 1061, 1095
BlsI GCNGC 7 cut(s) 632, 706, 767, 928, 1059, 1062, 1096
Bme1390I CCNGG 5 cut(s) 39, 723, 734, 782, 899
Bme18I GGWCC 2 cut(s) 880, 890
BmgT120I GGNCC 4 cut(s) 36, 702, 880, 890
BmrFI CCNGG 5 cut(s) 39, 723, 734, 782, 899
BmsI GCATC 1 cut(s) 937
BpuMI CCSGG 4 cut(s) 39, 723, 782, 899
Bsa29I ATCGAT 3 cut(s) 390, 477, 1123
BsaAI YACGTR 3 cut(s) 49, 92, 287
BsaBI GATNNNNATC 1 cut(s) 1119
BsaHI GRCGYC 2 cut(s) 281, 1247
BsaI GGTCTC 2 cut(s) 806, 896
BsaJI CCNNGG 9 cut(s) 178, 200, 443, 493, 598, 705, 722, 796, 876
BsaWI WCCGGW 3 cut(s) 295, 542, 856
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 542, 599
Bse118I RCCGGY 1 cut(s) 1053
Bse1I ACTGG 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 1119
BseAI TCCGGA 2 cut(s) 542, 856
BseBI CCWGG 1 cut(s) 734
BseCI ATCGAT 3 cut(s) 390, 477, 1123
BseDI CCNNGG 9 cut(s) 178, 200, 443, 493, 598, 705, 722, 796, 876
BseGI GGATG 2 cut(s) 210, 952
BseJI GATNNNNATC 1 cut(s) 1119
BseLI CCNNNNNNNGG 3 cut(s) 111, 542, 599
BseMII CTCAG 3 cut(s) 270, 367, 749
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 136, 655
BseXI GCAGC 4 cut(s) 913, 1044, 1047, 1106
BsgI GTGCAG 1 cut(s) 929
Bsh1236I CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
Bsh1285I CGRYCG 2 cut(s) 477, 688
BshFI GGCC 2 cut(s) 37, 704
BshNI GGYRCC 4 cut(s) 718, 1000, 1031, 1246
BshVI ATCGAT 3 cut(s) 390, 477, 1123
BsiEI CGRYCG 2 cut(s) 477, 688
BsiHKAI GWGCWC 2 cut(s) 560, 695
BslFI GGGAC 2 cut(s) 903, 1137
BslI CCNNNNNNNGG 3 cut(s) 111, 542, 599
BsmAI GTCTC 4 cut(s) 754, 806, 896, 987
BsmBI CGTCTC 2 cut(s) 754, 987
BsmFI GGGAC 2 cut(s) 903, 1137
BsnI GGCC 2 cut(s) 37, 704
Bso31I GGTCTC 2 cut(s) 806, 896
Bsp1286I GDGCHC 2 cut(s) 560, 695
Bsp13I TCCGGA 2 cut(s) 542, 856
Bsp19I CCATGG 1 cut(s) 598
Bsp68I TCGCGA 1 cut(s) 684
BspANI GGCC 2 cut(s) 37, 704
BspCNI CTCAG 3 cut(s) 269, 366, 750
BspDI ATCGAT 3 cut(s) 390, 477, 1123
BspEI TCCGGA 2 cut(s) 542, 856
BspFNI CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
BspPI GGATC 6 cut(s) 553, 748, 848, 867, 880, 970
BspT107I GGYRCC 4 cut(s) 718, 1000, 1031, 1246
BspTNI GGTCTC 2 cut(s) 806, 896
BsrBI CCGCTC 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 1053
BsrI ACTGG 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 1053
BssECI CCNNGG 9 cut(s) 178, 200, 443, 493, 598, 705, 722, 796, 876
BssNI GRCGYC 2 cut(s) 281, 1247
BssT1I CCWWGG 2 cut(s) 178, 598
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 4 cut(s) 190, 263, 884, 907
Bst6I CTCTTC 1 cut(s) 632
BstACI GRCGYC 2 cut(s) 281, 1247
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBAI YACGTR 3 cut(s) 49, 92, 287
BstC8I GCNNGC 4 cut(s) 327, 817, 830, 1055
BstDEI CTNAG 4 cut(s) 256, 353, 758, 1305
BstDSI CCRYGG 4 cut(s) 200, 598, 705, 876
BstF5I GGATG 2 cut(s) 210, 952
BstFNI CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
BstH2I RGCGCY 2 cut(s) 972, 1250
BstHHI GCGC 3 cut(s) 254, 971, 1249
BstMAI GTCTC 4 cut(s) 754, 806, 896, 987
BstMCI CGRYCG 2 cut(s) 477, 688
BstMWI GCNNNNNNNGC 9 cut(s) 121, 251, 331, 690, 710, 825, 956, 977, 1050
BstNI CCWGG 1 cut(s) 734
BstSCI CCNGG 5 cut(s) 37, 721, 732, 780, 897
BstUI CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
BstV1I GCAGC 4 cut(s) 913, 1044, 1047, 1106
BstX2I RGATCY 2 cut(s) 753, 872
BstYI RGATCY 2 cut(s) 753, 872
Bsu15I ATCGAT 3 cut(s) 390, 477, 1123
BsuRI GGCC 2 cut(s) 37, 704
BsuTUI ATCGAT 3 cut(s) 390, 477, 1123
BtgI CCRYGG 4 cut(s) 200, 598, 705, 876
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 2 cut(s) 210, 952
BtsI GCAGTG 1 cut(s) 1004
BtsIMutI CAGTG 2 cut(s) 174, 1004
BtuMI TCGCGA 1 cut(s) 684
Cac8I GCNNGC 4 cut(s) 327, 817, 830, 1055
CfoI GCGC 3 cut(s) 254, 971, 1249
Cfr10I RCCGGY 1 cut(s) 1053
Cfr13I GGNCC 4 cut(s) 36, 702, 880, 890
Cfr42I CCGCGG 2 cut(s) 203, 708
ClaI ATCGAT 3 cut(s) 390, 477, 1123
CpoI CGGWCCG 1 cut(s) 880
CseI GACGC 3 cut(s) 113, 771, 969
CsiI ACCWGGT 1 cut(s) 732
CspI CGGWCCG 1 cut(s) 880
CviAII CATG 2 cut(s) 80, 599
DdeI CTNAG 4 cut(s) 256, 353, 758, 1305
DinI GGCGCC 1 cut(s) 1248
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
EciI GGCGGA 3 cut(s) 494, 975, 1040
Ecl136II GAGCTC 1 cut(s) 558
Eco130I CCWWGG 2 cut(s) 178, 598
Eco24I GRGCYC 1 cut(s) 560
Eco31I GGTCTC 2 cut(s) 806, 896
Eco47I GGWCC 2 cut(s) 880, 890
Eco47III AGCGCT 1 cut(s) 970
Eco53kI GAGCTC 1 cut(s) 558
Eco57I CTGAAG 1 cut(s) 641
Eco72I CACGTG 2 cut(s) 92, 287
EcoICRI GAGCTC 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 890
EcoRII CCWGG 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 178, 598
EcoT38I GRGCYC 1 cut(s) 560
EgeI GGCGCC 1 cut(s) 1248
EheI GGCGCC 1 cut(s) 1248
ErhI CCWWGG 2 cut(s) 178, 598
Esp3I CGTCTC 2 cut(s) 754, 987
FaeI CATG 2 cut(s) 83, 602
FaiI YATR 7 cut(s) 81, 309, 341, 600, 944, 1256, 1270
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 2 cut(s) 903, 1137
FatI CATG 2 cut(s) 79, 598
FauI CCCGC 3 cut(s) 250, 545, 824
Fnu4HI GCNGC 7 cut(s) 631, 705, 766, 927, 1058, 1061, 1095
FokI GGATG 2 cut(s) 217, 959
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 7 cut(s) 631, 705, 766, 927, 1058, 1061, 1095
FspBI CTAG 1 cut(s) 1011
GlaI GCGC 3 cut(s) 253, 970, 1248
GluI GCNGC 7 cut(s) 631, 705, 766, 927, 1058, 1061, 1095
HaeII RGCGCY 2 cut(s) 972, 1250
HaeIII GGCC 2 cut(s) 37, 704
HgaI GACGC 3 cut(s) 113, 771, 969
HhaI GCGC 3 cut(s) 254, 971, 1249
Hin1I GRCGYC 2 cut(s) 281, 1247
Hin1II CATG 2 cut(s) 83, 602
Hin6I GCGC 3 cut(s) 252, 969, 1247
HinP1I GCGC 3 cut(s) 252, 969, 1247
HindIII AAGCTT 1 cut(s) 1302
HinfI GANTC 5 cut(s) 20, 467, 643, 1065, 1106
HphI GGTGA 1 cut(s) 748
Hpy166II GTNNAC 1 cut(s) 1283
Hpy188I TCNGA 8 cut(s) 259, 356, 444, 474, 494, 648, 697, 806
Hpy188III TCNNGA 8 cut(s) 482, 543, 549, 683, 751, 757, 857, 1178
Hpy8I GTNNAC 1 cut(s) 1283
Hpy99I CGWCG 2 cut(s) 129, 283
HpyAV CCTTC 7 cut(s) 546, 709, 829, 838, 1017, 1205, 1260
HpyCH4III ACNGT 4 cut(s) 190, 263, 884, 907
HpyCH4IV ACGT 4 cut(s) 48, 91, 281, 286
HpyCH4V TGCA 5 cut(s) 214, 334, 910, 1204, 1272
HpyF10VI GCNNNNNNNGC 9 cut(s) 121, 251, 331, 690, 710, 825, 956, 977, 1050
HpyF3I CTNAG 4 cut(s) 256, 353, 758, 1305
HpySE526I ACGT 4 cut(s) 48, 91, 281, 286
Hsp92I GRCGYC 2 cut(s) 281, 1247
Hsp92II CATG 2 cut(s) 83, 602
HspAI GCGC 3 cut(s) 252, 969, 1247
KasI GGCGCC 1 cut(s) 1246
KflI GGGWCCC 1 cut(s) 890
Kpn2I TCCGGA 2 cut(s) 542, 856
KroI GCCGGC 1 cut(s) 1053
KroNI GCCGGC 1 cut(s) 1055
KspI CCGCGG 2 cut(s) 203, 708
LmnI GCTCC 3 cut(s) 545, 555, 608
Lsp1109I GCAGC 4 cut(s) 913, 1044, 1047, 1106
LweI GCATC 1 cut(s) 937
MabI ACCWGGT 1 cut(s) 732
MaeI CTAG 1 cut(s) 1011
MaeII ACGT 4 cut(s) 48, 91, 281, 286
MaeIII GTNAC 2 cut(s) 282, 291
MbiI CCGCTC 1 cut(s) 540
MflI RGATCY 2 cut(s) 753, 872
MhlI GDGCHC 2 cut(s) 560, 695
MluI ACGCGT 1 cut(s) 978
Mly113I GGCGCC 1 cut(s) 1247
MlyI GAGTC 2 cut(s) 652, 1059
MmeI TCCRAC 1 cut(s) 1271
MroI TCCGGA 2 cut(s) 542, 856
MroNI GCCGGC 1 cut(s) 1053
MseI TTAA 4 cut(s) 237, 386, 417, 921
MspA1I CMGCKG 2 cut(s) 202, 707
MspR9I CCNGG 5 cut(s) 39, 723, 734, 782, 899
MvaI CCWGG 1 cut(s) 734
MvnI CGCG 8 cut(s) 202, 252, 278, 684, 707, 765, 819, 980
MwoI GCNNNNNNNGC 9 cut(s) 121, 251, 331, 690, 710, 825, 956, 977, 1050
NaeI GCCGGC 1 cut(s) 1055
NarI GGCGCC 1 cut(s) 1247
NciI CCSGG 4 cut(s) 39, 723, 782, 899
NcoI CCATGG 1 cut(s) 598
NgoMIV GCCGGC 1 cut(s) 1053
NlaIII CATG 2 cut(s) 83, 602
NmuCI GTSAC 1 cut(s) 282
NruI TCGCGA 1 cut(s) 684
PdiI GCCGGC 1 cut(s) 1055
PfeI GAWTC 3 cut(s) 20, 467, 1106
PkrI GCNGC 7 cut(s) 632, 706, 767, 928, 1059, 1062, 1096
Ple19I CGATCG 2 cut(s) 477, 688
PleI GAGTC 2 cut(s) 651, 1059
PluTI GGCGCC 1 cut(s) 1250
PmaCI CACGTG 2 cut(s) 92, 287
PmlI CACGTG 2 cut(s) 92, 287
PpsI GAGTC 2 cut(s) 651, 1059
Ppu21I YACGTR 3 cut(s) 49, 92, 287
PpuMI RGGWCCY 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 890
Psp6I CCWGG 1 cut(s) 732
PspCI CACGTG 2 cut(s) 92, 287
PspGI CCWGG 1 cut(s) 732
PspPI GGNCC 4 cut(s) 36, 702, 880, 890
PspPPI RGGWCCY 1 cut(s) 890
PsrI GAACNNNNNNTAC 2 cut(s) 1126, 1158
PsuI RGATCY 2 cut(s) 753, 872
PvuI CGATCG 2 cut(s) 477, 688
RruI TCGCGA 1 cut(s) 684
Rsr2I CGGWCCG 1 cut(s) 880
RsrII CGGWCCG 1 cut(s) 880
SacI GAGCTC 1 cut(s) 560
SacII CCGCGG 2 cut(s) 203, 708
SaqAI TTAA 4 cut(s) 237, 386, 417, 921
SatI GCNGC 7 cut(s) 631, 705, 766, 927, 1058, 1061, 1095
Sau96I GGNCC 4 cut(s) 36, 702, 880, 890
SchI GAGTC 2 cut(s) 652, 1059
ScrFI CCNGG 5 cut(s) 39, 723, 734, 782, 899
SduI GDGCHC 2 cut(s) 560, 695
SexAI ACCWGGT 1 cut(s) 732
SfaNI GCATC 1 cut(s) 937
SfoI GGCGCC 1 cut(s) 1248
Sfr303I CCGCGG 2 cut(s) 203, 708
SgrBI CCGCGG 2 cut(s) 203, 708
SinI GGWCC 2 cut(s) 880, 890
SspDI GGCGCC 1 cut(s) 1246
SspI AATATT 1 cut(s) 616
SspMI CTAG 1 cut(s) 1011
SstI GAGCTC 1 cut(s) 560
StyD4I CCNGG 5 cut(s) 37, 721, 732, 780, 897
StyI CCWWGG 2 cut(s) 178, 598
TaaI ACNGT 4 cut(s) 190, 263, 884, 907
TaiI ACGT 4 cut(s) 51, 94, 284, 289
TaqI TCGA 9 cut(s) 53, 127, 390, 477, 965, 1068, 1117, 1123, 1189
TaqII GACCGA 1 cut(s) 215
TauI GCSGC 3 cut(s) 633, 707, 768
TfiI GAWTC 3 cut(s) 20, 467, 1106
Tru1I TTAA 4 cut(s) 237, 386, 417, 921
Tru9I TTAA 4 cut(s) 237, 386, 417, 921
TscAI CASTG 2 cut(s) 174, 1011
TseFI GTSAC 1 cut(s) 282
TseI GCWGC 4 cut(s) 926, 1057, 1060, 1094
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 2 cut(s) 68, 608
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 2 cut(s) 174, 1011
VpaK11BI GGWCC 2 cut(s) 880, 890
XapI RAATTY 2 cut(s) 364, 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XspI CTAG 1 cut(s) 1011
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.