pycom17g22950
HSP70 Family

Heat shock 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003935
Physical Location & Seq
Forward (+)
21353187 .. 21353911
725 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22950.1

Sequence Viewer

Length: 546 bp
ATGGTGCAGGAGGCAGAGAGGTACAAGGCTGAAGATGAGGAGGTTAAGAAGAAGGTGGATGCCAAGAACTCGCTTGAGAACTACGCATACAACATGCGGAACACCGTCAAAGATGAGCAGATTGCTGGAAAATTAGACCCTGCAGACAAGCAGAAGATTGAGAAGGCCATTGACGAGGCAATCGAGTGGCTAGACCGGAACCAGTTGGCGGAGGTGGAGGAATTTGAGGACAAGCAGAAGGAGTTGGAGGGATTGTGCAACCCGATCATTGCCAAAATGTATCAGGGTCCTGGAGGAAATGTGCCGATGGGCGGTGGTGCTCAGATGCCTGGTAGCGGATTTGGAAACACTACGTCCGGTGGCGGATCAGGAGCCGGGCCTACGATTGAGGAAAATATGTTTATTGCTCGATTAGTTGTGTGGTGCAATGGTTCATTAGGATTTAGGGTTTTTGGTGGTCTGTTAAAGCCACTCATTTGTGGTGGTTCCACTGCCAAATGTTTTAAGAGGTACCCACGTAATTTGCTCCATCATAAAATGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

182

Amino Acids

19.94

Weight (kDa)

5.99

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 95 2.3e-11 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AciI CCGC 5 cut(s) 97, 209, 312, 336, 363
AclWI GGATC 1 cut(s) 373
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 2 cut(s) 23, 512
AfiI CCNNNNNNNGG 3 cut(s) 208, 311, 335
AjnI CCWGG 2 cut(s) 289, 328
Alw21I GWGCWC 1 cut(s) 322
AlwI GGATC 1 cut(s) 373
AoxI GGCC 2 cut(s) 165, 377
ApoI RAATTY 1 cut(s) 221
Asp718I GGTACC 1 cut(s) 510
AspS9I GGNCC 2 cut(s) 287, 377
AsuC2I CCSGG 1 cut(s) 376
AvaII GGWCC 1 cut(s) 287
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BanI GGYRCC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 322
BccI CCATC 2 cut(s) 301, 537
BciT130I CCWGG 2 cut(s) 291, 330
BcnI CCSGG 1 cut(s) 376
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 141
Bme1390I CCNGG 3 cut(s) 291, 330, 376
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 287, 377
BmiI GGNNCC 5 cut(s) 200, 288, 373, 487, 512
BmrFI CCNGG 3 cut(s) 291, 330, 376
BmsI GCATC 2 cut(s) 49, 315
BpmI CTGGAG 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 376
BsaAI YACGTR 1 cut(s) 518
BsaWI WCCGGW 2 cut(s) 195, 356
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 285, 315
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 311, 335
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 267, 433
BseBI CCWGG 2 cut(s) 291, 330
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 3 cut(s) 208, 311, 335
BseMI GCAATG 2 cut(s) 267, 433
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 2 cut(s) 167, 379
BshNI GGYRCC 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 3 cut(s) 196, 357, 375
BslI CCNNNNNNNGG 3 cut(s) 208, 311, 335
BsnI GGCC 2 cut(s) 167, 379
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 2 cut(s) 264, 365
BspACI CCGC 5 cut(s) 97, 209, 312, 336, 363
BspANI GGCC 2 cut(s) 167, 379
BspCNI CTCAG 1 cut(s) 334
BspLI GGNNCC 5 cut(s) 200, 288, 373, 487, 512
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 267, 433
BsrI ACTGG 1 cut(s) 202
BssMI GATC 2 cut(s) 264, 365
Bst2UI CCWGG 2 cut(s) 291, 330
Bst4CI ACNGT 1 cut(s) 106
BstBAI YACGTR 1 cut(s) 518
BstDEI CTNAG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 2 cut(s) 267, 368
BstMBI GATC 2 cut(s) 264, 365
BstNI CCWGG 2 cut(s) 291, 330
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 3 cut(s) 289, 328, 374
BstSFI CTRYAG 1 cut(s) 141
BsuRI GGCC 2 cut(s) 167, 379
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 287, 377
Csp6I GTAC 2 cut(s) 22, 511
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviAII CATG 1 cut(s) 94
CviJI RGCY 6 cut(s) 29, 167, 190, 374, 379, 469
CviKI_1 RGCY 6 cut(s) 29, 167, 190, 374, 379, 469
CviQI GTAC 2 cut(s) 22, 511
DdeI CTNAG 1 cut(s) 321
DpnI GATC 2 cut(s) 266, 367
DpnII GATC 2 cut(s) 264, 365
EciI GGCGGA 2 cut(s) 224, 378
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 289, 328
FaeI CATG 1 cut(s) 97
FaiI YATR 4 cut(s) 88, 95, 398, 534
FatI CATG 1 cut(s) 93
FokI GGATG 1 cut(s) 71
FspBI CTAG 1 cut(s) 191
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 2 cut(s) 167, 379
HapII CCGG 3 cut(s) 196, 357, 375
Hin1II CATG 1 cut(s) 97
HpaII CCGG 3 cut(s) 196, 357, 375
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 46, 157, 232
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 2 cut(s) 353, 517
HpyCH4V TGCA 5 cut(s) 7, 143, 258, 426, 541
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 2 cut(s) 353, 517
Hsp92II CATG 1 cut(s) 97
KpnI GGTACC 1 cut(s) 514
Kzo9I GATC 2 cut(s) 264, 365
LmnI GCTCC 2 cut(s) 371, 531
LweI GCATC 2 cut(s) 49, 315
MaeI CTAG 1 cut(s) 191
MaeII ACGT 2 cut(s) 353, 517
MalI GATC 2 cut(s) 266, 367
MboI GATC 2 cut(s) 264, 365
MboII GAAGA 3 cut(s) 44, 61, 166
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 3 cut(s) 131, 221, 520
MmeI TCCRAC 1 cut(s) 225
MseI TTAA 3 cut(s) 45, 464, 504
MspI CCGG 3 cut(s) 196, 357, 375
MspR9I CCNGG 3 cut(s) 291, 330, 376
MvaI CCWGG 2 cut(s) 291, 330
NciI CCSGG 1 cut(s) 376
NdeII GATC 2 cut(s) 264, 365
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 5 cut(s) 200, 288, 373, 487, 512
NspI RCATGY 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 518
PpuMI RGGWCCY 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 289, 328
PspGI CCWGG 2 cut(s) 289, 328
PspN4I GGNNCC 5 cut(s) 200, 288, 373, 487, 512
PspPI GGNCC 2 cut(s) 287, 377
PspPPI RGGWCCY 1 cut(s) 287
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 145
RsaI GTAC 2 cut(s) 23, 512
RsaNI GTAC 2 cut(s) 22, 511
SaqAI TTAA 3 cut(s) 45, 464, 504
Sau3AI GATC 2 cut(s) 264, 365
Sau96I GGNCC 2 cut(s) 287, 377
ScrFI CCNGG 3 cut(s) 291, 330, 376
SduI GDGCHC 1 cut(s) 322
SetI ASST 7 cut(s) 23, 45, 57, 216, 356, 512, 520
SfaNI GCATC 2 cut(s) 49, 315
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 3 cut(s) 131, 221, 520
SsiI CCGC 5 cut(s) 97, 209, 312, 336, 363
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 3 cut(s) 289, 328, 374
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 2 cut(s) 356, 520
TaqI TCGA 2 cut(s) 183, 409
TasI AATT 3 cut(s) 131, 221, 520
Tru1I TTAA 3 cut(s) 45, 464, 504
Tru9I TTAA 3 cut(s) 45, 464, 504
TscAI CASTG 1 cut(s) 496
TspDTI ATGAA 1 cut(s) 423
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 97
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.