Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0004093

Physical Location & Seq

Genomic Coordinates Reverse (-)

24752315 .. 24754852

Gene Structure 2538 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g26470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGAGTTACAAACAAAACTGGTTTGGATCCAGGTTGACCCGACCCTTTACAGTGGCTAACCGTTACGCAGCTCAGCTGCAGCTATGCTGCCCCCAAAGCCCCACCTCTCACTCTTTGGCTCGAACCATCCACGCCCACATGATCGCTTCTGGGTTCAGTCCACGTGGCCATATTCTTAACCGTTTGATTGATATTTACTGCAAATCTTCAAATATTAGGTACGCCCGCCAACTGTTTGATAAAATTCCCAAACCAGACATCGTTGCTCGAACAACCTTGGTTAAGGCATACTCTGTCATTGGAAATCTGACGCTGGCCCGTAAAATATTCGATGAAACCCCGTTCAGCATGCGAGACACTGTTTCTTACAATGCCATGATCACTGGCTATTCCCATAACAACGATGGCTATGCCGCTGTTAGACTGTTCTGCGACATGAGGCGAGGAGATATTAGGCCTGACGATTTCACCTTCACGTGTGTTCTTAGTGCTTCGGCGCAGATAGTGGATGACGAGAAGCAGTGCAAGCAACTGCATTGTGGAATAGTTAAGTCTGGAACGGCGTTTGCCACTTCGGTGTGGAATGCGCTATTATCTGTTTATGTTAAATGTGCTTCCTCGCCATTGGTATCATCGTCATCGTTGATGAGGGAAGCTAGGAAATTGTTCGATGAGATGCCTAAGAGAGATGAACTGTCATGGACGACAATGATTACTGGGTACGTAAGGAATGAAGACCTTGATGCAGCTCGTGAGTTGCTGGACGGGATGGATGAAAAGTTGGAAGTTGCATGGAATGCCATGATTTCGGGTTATGTGCGTCATGATTCTTTCCAAGAAGCATTGTTGTTGTTTAGGAAAATGCGTTTGCTGGGGATTCGTCAGGATGAGTTTACATACACAAGTGTTATCAGTGCTTGTGCTAATATTGGATTATTTCAGTTGGGGAAGCAGGTGCATGCTTACATACTAAGAACCGAAGCAAAGCCCACTCTGGATTTTTCATTATCTGTTAACAATGCATTGATTACACTATATTATAAATGCCGTAAACTTGATGAAGCACGATATATTTTTAATAATATGCCTGTTAGAGATCTTGTTTCTTGGAATGCAATACTGTCAGGGTATGTAAATGCAGGGCGCATTCAGGAAGCAAAATATTTTTTCGAGGAAATGCCAGAGAGGAGCATTCTAACATGGACTGTGATAATATCTGGTTTAGCTCAAAACGGATTTGGAGAAGAGGCTATGAAGCTATTCAACCAAATGAGATTAGAGGGTTGTGAACCATGTGATTATGCATTTGCTGGAGCCATTACTTCTTGTGCTGCGCTGGGAGCATTGAAGCATGGACTCCAGCTCCATGCTCAACTGATTAGGTTGGGGCATGATTCAAGCCTTTCAGCTGCAAATGCACTTATCACGATGTATGGCAGATGCGGAGTTGTTGAGGATGCAAACATTTTGTTCCTCACGATGTCTTATATTGATTCAGTGTCTTGGAATGCTATGATTGCAGCTCTCGCACAGCATGGATATGGTGTCCAAGCGGTAGGACTTTTTGAGCGAATGTTGAAGGAGGATATGCTACCAGATAGAGTAACTTTCCTCATCATTCTCTCAGCTTGTAGTCATGCTGGTTTAGTCAAAGAAGGGCGCCATTATTTTAGTTCAATGCGTGATTCTTATGGTATTAGCCCTGATGAAGAACATTATGCTCGCATGATTGATCTGTTGTGTCGGTGCGGGGAGTTCACAGAAGCCAAGGACTTGATTGAATCAATGCCTTTTGAGCCAGGTGCGCCGATATGGGAGGCTCTTCTTGCTGGTTGCAAGACTCATGGGAATCTGGATTTAGGAATTCAAGCTGCAGAACGACTCTTGGAGTTGGTGCCACAGCATGATGGAACCTACGTACTGTTGTCAAACTTGTATGCCACCGCAGGCTATTGGGATGATGTGGCTAAGGTACGGAAACTAATGAGAGATCGAGGGGTTAAGAAAGAGCCTGGTTGTAGTTGGATTGACGTTGAAAACATGGTCCACGTCTTCTTGGTTGATGACACAAAGCACCCTGAGGTGCAAGCAGTGTACAAGTATCTGGAGCAACTAGGACTTGAAATGAGGAAATTAGGATATGTACCGGACACAAATTATGTGTTGCATGATATGGAGTCCGAGCATAAGGAGTATGCCATTTCCACTCACAGTGAGAAGCTTGCGGTTGCATTTGGGCTCATGAAGCTTCCTCCTGGACACACAATCAGAGTATTTAAGAATCTTAGGATTTGTGGGGATTGCCATAATGCATTCAAGTTTATATCAAGAGTGGTAGGGAGAGAAATAATTGTGAGAGATGGGAAGAGGTTTCATCATTTTAGGAACGGTGAATGCTCTTGTGGCGATTACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

806

Amino Acids

90.91

Weight (kDa)

6.95

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	118 - 164	6e-13	PPR repeat family
PPR_2	PF13041	263 - 309	8.2e-12	PPR repeat family
PPR	PF01535	263 - 293	1.7e-07	PPR repeat
PPR_2	PF13041	366 - 398	1.7e-06	PPR repeat family
PPR	PF01535	368 - 397	2.2e-06	PPR repeat
PPR_2	PF13041	397 - 443	5.9e-08	PPR repeat family
PPR	PF01535	400 - 429	5.1e-08	PPR repeat
PPR_2	PF13041	498 - 545	4.6e-10	PPR repeat family
PPR	PF01535	500 - 528	5.9e-06	PPR repeat
E_motif	PF20431	615 - 677	1.6e-23	E motif
DYW_deaminase	PF14432	713 - 805	2e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004093)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25360
fragaria_vesca	FvH4_5g32410 FvH4_6g08990
malus_domestica	MD17G1265000.v1.1
prunus_persica	Prupe.3G114900_v2.0.a1 Prupe.3G115100_v2.0.a1
pyrus_communis	pycom17g26470
rosa_chinensis	RchiOBHm_Chr7g0221631 RchiOBHm_Chr7g0228891 RchiOBHm_Chr7g0228961
rosa_laevigata	RLG00000001538 RLG00000002149
rosa_multiflora	Rmu_sc0005016.1_g000036 Rmu_sc0005913.1_g000007 Rmu_sc0005913.1_g000028 Rmu_sc0027650.1_g000001
rosa_roxburghii	Rroxscaffold_3G00230820 Rroxscaffold_3G00238170
rosa_rugosa	Rorug07G0202700 Rorug07G0202700 Rorug07G0252700 Rorug07G0254200
rosa_samantha	Rh7AG343100 Rh7AG405900 Rh7BG333100 Rh7BG385400 Rh7CG360700 Rh7CG423700 Rh7DG341100
rosa_wichuraiana	Rw0G004030 Rw7G029100 Rw7G033660 Rw7G033680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1041
AarI	CACCTGC	1 cut(s)	943
Acc36I	ACCTGC	1 cut(s)	943
AccB1I	GGYRCC	2 cut(s)	1659, 1894
AciI	CCGC	7 cut(s)	226, 414, 1443, 1553, 1749, 1944, 2225
AclWI	GGATC	2 cut(s)	21, 34
AcoI	YGGCCR	1 cut(s)	166
AcsI	RAATTY	2 cut(s)	243, 1863
AcvI	CACGTG	2 cut(s)	164, 477
AcyI	GRCGYC	1 cut(s)	1660
AfaI	GTAC	6 cut(s)	221, 722, 1920, 1974, 2096, 2145
AflIII	ACRYGT	1 cut(s)	476
AjiI	CACGTC	1 cut(s)	2050
AjnI	CCWGG	4 cut(s)	29, 1798, 2011, 2254
AleI	CACNNNNGTG	1 cut(s)	575
Alw26I	GTCTC	1 cut(s)	348
AlwI	GGATC	2 cut(s)	21, 34
AoxI	GGCC	3 cut(s)	166, 315, 455
ApeKI	GCWGC	9 cut(s)	68, 76, 79, 87, 746, 1331, 1409, 1520, 1871
ApoI	RAATTY	2 cut(s)	243, 1863
Asp700I	GAANNNNTTC	2 cut(s)	665, 1259
AspLEI	GCGC	6 cut(s)	499, 589, 1146, 1336, 1662, 1807
AspS9I	GGNCC	2 cut(s)	316, 2044
AsuHPI	GGTGA	2 cut(s)	460, 2402
AvaII	GGWCC	1 cut(s)	2044
AxyI	CCTNAGG	1 cut(s)	2079
BalI	TGGCCA	1 cut(s)	168
BamHI	GGATCC	1 cut(s)	26
BanI	GGYRCC	2 cut(s)	1659, 1894
BanII	GRGCYC	1 cut(s)	2241
BauI	CACGAG	1 cut(s)	750
BbrPI	CACGTG	2 cut(s)	164, 477
BbsI	GAAGAC	2 cut(s)	741, 2044
BbvI	GCAGC	9 cut(s)	63, 74, 80, 91, 758, 1318, 1396, 1532, 1858
BccI	CCATC	5 cut(s)	134, 398, 763, 1901, 2354
BceAI	ACGGC	2 cut(s)	576, 1032
BcgI	CGANNNNNNTGC	2 cut(s)	392, 426
BciT130I	CCWGG	4 cut(s)	31, 1800, 2013, 2256
BclI	TGATCA	1 cut(s)	378
BcoDI	GTCTC	1 cut(s)	348
BfaI	CTAG	2 cut(s)	657, 2114
BfmI	CTRYAG	2 cut(s)	77, 1872
BfoI	RGCGCY	1 cut(s)	1663
BfuAI	ACCTGC	1 cut(s)	943
BglII	AGATCT	1 cut(s)	1096
BlpI	GCTNAGC	1 cut(s)	72
Bme1390I	CCNGG	4 cut(s)	31, 1800, 2013, 2256
Bme18I	GGWCC	1 cut(s)	2044
BmgBI	CACGTC	1 cut(s)	2050
BmgT120I	GGNCC	2 cut(s)	316, 2044
BmiI	GGNNCC	5 cut(s)	28, 1315, 1661, 1896, 1912
BmrFI	CCNGG	4 cut(s)	31, 1800, 2013, 2256
BmrI	ACTGGG	1 cut(s)	726
BmsI	GCATC	4 cut(s)	666, 733, 1430, 1447
BmuI	ACTGGG	1 cut(s)	726
BpiI	GAAGAC	2 cut(s)	741, 2044
BpmI	CTGGAG	3 cut(s)	1332, 1343, 2126
Bpu10I	CCTNAGC	1 cut(s)	1968
Bpu1102I	GCTNAGC	1 cut(s)	72
BsaAI	YACGTR	4 cut(s)	164, 477, 724, 1918
BsaHI	GRCGYC	1 cut(s)	1660
BsaJI	CCNNGG	2 cut(s)	276, 1767
BsaWI	WCCGGW	1 cut(s)	2146
BsaXI	ACNNNNNCTCC	2 cut(s)	1347, 1377
Bse1I	ACTGG	4 cut(s)	23, 388, 721, 2417
Bse21I	CCTNAGG	1 cut(s)	2079
BseBI	CCWGG	4 cut(s)	31, 1800, 2013, 2256
BseDI	CCNNGG	2 cut(s)	276, 1767
BseGI	GGATG	7 cut(s)	126, 514, 774, 778, 892, 1462, 1963
BseMII	CTCAG	3 cut(s)	86, 1638, 2070
BseNI	ACTGG	4 cut(s)	23, 388, 721, 2417
BseRI	GAGGAG	2 cut(s)	459, 1201
BseXI	GCAGC	9 cut(s)	63, 74, 80, 91, 758, 1318, 1396, 1532, 1858
BseYI	CCCAGC	2 cut(s)	871, 1336
BshFI	GGCC	3 cut(s)	168, 317, 457
BshNI	GGYRCC	2 cut(s)	1659, 1894
BsiSI	CCGG	1 cut(s)	2147
BsmAI	GTCTC	1 cut(s)	348
BsmI	GAATGC	8 cut(s)	589, 802, 1117, 1146, 1191, 1513, 2312, 2399
BsnI	GGCC	3 cut(s)	168, 317, 457
Bsp1286I	GDGCHC	1 cut(s)	2241
Bsp1407I	TGTACA	1 cut(s)	2094
Bsp143I	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
Bsp1720I	GCTNAGC	1 cut(s)	72
BspACI	CCGC	7 cut(s)	226, 414, 1443, 1553, 1749, 1944, 2225
BspANI	GGCC	3 cut(s)	168, 317, 457
BspCNI	CTCAG	3 cut(s)	85, 1637, 2071
BspHI	TCATGA	2 cut(s)	823, 2241
BspLI	GGNNCC	5 cut(s)	28, 1315, 1661, 1896, 1912
BspMAI	CTGCAG	2 cut(s)	81, 1876
BspMI	ACCTGC	1 cut(s)	943
BspPI	GGATC	2 cut(s)	21, 34
BspQI	GCTCTTC	1 cut(s)	1827
BspT107I	GGYRCC	2 cut(s)	1659, 1894
BsrGI	TGTACA	1 cut(s)	2094
BsrI	ACTGG	4 cut(s)	23, 388, 721, 2417
BssECI	CCNNGG	2 cut(s)	276, 1767
BssMI	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
BssNI	GRCGYC	1 cut(s)	1660
BssSI	CACGAG	1 cut(s)	750
BssT1I	CCWWGG	2 cut(s)	276, 1767
Bst2BI	CACGAG	1 cut(s)	750
Bst2UI	CCWGG	4 cut(s)	31, 1800, 2013, 2256
Bst6I	CTCTTC	3 cut(s)	1239, 1827, 2360
BstACI	GRCGYC	1 cut(s)	1660
BstAPI	GCANNNNNTGC	1 cut(s)	797
BstAUI	TGTACA	1 cut(s)	2094
BstBAI	YACGTR	4 cut(s)	164, 477, 724, 1918
BstC8I	GCNNGC	9 cut(s)	226, 315, 350, 527, 960, 1723, 1948, 2088, 2223
BstDEI	CTNAG	8 cut(s)	72, 485, 681, 971, 1624, 1968, 2079, 2285
BstF5I	GGATG	7 cut(s)	126, 514, 774, 778, 892, 1462, 1963
BstH2I	RGCGCY	1 cut(s)	1663
BstHHI	GCGC	6 cut(s)	499, 589, 1146, 1336, 1662, 1807
BstKTI	GATC	6 cut(s)	29, 144, 381, 1099, 1735, 1993
BstMAI	GTCTC	1 cut(s)	348
BstMBI	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
BstNI	CCWGG	4 cut(s)	31, 1800, 2013, 2256
BstNSI	RCATGY	2 cut(s)	352, 962
BstSCI	CCNGG	4 cut(s)	29, 1798, 2011, 2254
BstSFI	CTRYAG	2 cut(s)	77, 1872
BstSNI	TACGTA	2 cut(s)	724, 1918
BstV1I	GCAGC	9 cut(s)	63, 74, 80, 91, 758, 1318, 1396, 1532, 1858
BstV2I	GAAGAC	2 cut(s)	741, 2044
BstX2I	RGATCY	2 cut(s)	26, 1096
BstYI	RGATCY	2 cut(s)	26, 1096
Bsu36I	CCTNAGG	1 cut(s)	2079
BsuRI	GGCC	3 cut(s)	168, 317, 457
BtrI	CACGTC	1 cut(s)	2050
BtsCI	GGATG	7 cut(s)	126, 514, 774, 778, 892, 1462, 1963
BtsI	GCAGTG	2 cut(s)	527, 2097
BtsIMutI	CAGTG	8 cut(s)	57, 357, 381, 527, 919, 1503, 2097, 2218
BveI	ACCTGC	1 cut(s)	943
Cac8I	GCNNGC	9 cut(s)	226, 315, 350, 527, 960, 1723, 1948, 2088, 2223
CciI	TCATGA	2 cut(s)	823, 2241
CfoI	GCGC	6 cut(s)	499, 589, 1146, 1336, 1662, 1807
Cfr13I	GGNCC	2 cut(s)	316, 2044
CseI	GACGC	2 cut(s)	319, 809
Csp6I	GTAC	6 cut(s)	220, 721, 1919, 1973, 2095, 2144
CviQI	GTAC	6 cut(s)	220, 721, 1919, 1973, 2095, 2144
DdeI	CTNAG	8 cut(s)	72, 485, 681, 971, 1624, 1968, 2079, 2285
DinI	GGCGCC	1 cut(s)	1661
DpnI	GATC	6 cut(s)	28, 143, 380, 1098, 1734, 1992
DpnII	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
EaeI	YGGCCR	1 cut(s)	166
Eam1104I	CTCTTC	3 cut(s)	1239, 1827, 2360
EarI	CTCTTC	3 cut(s)	1239, 1827, 2360
Eco105I	TACGTA	2 cut(s)	724, 1918
Eco130I	CCWWGG	2 cut(s)	276, 1767
Eco147I	AGGCCT	1 cut(s)	457
Eco24I	GRGCYC	1 cut(s)	2241
Eco47I	GGWCC	1 cut(s)	2044
Eco72I	CACGTG	2 cut(s)	164, 477
Eco81I	CCTNAGG	1 cut(s)	2079
EcoRI	GAATTC	1 cut(s)	1863
EcoRII	CCWGG	4 cut(s)	29, 1798, 2011, 2254
EcoT14I	CCWWGG	2 cut(s)	276, 1767
EcoT22I	ATGCAT	3 cut(s)	1024, 1306, 2314
EcoT38I	GRGCYC	1 cut(s)	2241
EgeI	GGCGCC	1 cut(s)	1661
EheI	GGCGCC	1 cut(s)	1661
ErhI	CCWWGG	2 cut(s)	276, 1767
FauI	CCCGC	2 cut(s)	233, 1742
FbaI	TGATCA	1 cut(s)	378
FokI	GGATG	7 cut(s)	113, 521, 781, 785, 899, 1469, 1970
FriOI	GRGCYC	1 cut(s)	2241
FspBI	CTAG	2 cut(s)	657, 2114
GlaI	GCGC	6 cut(s)	498, 588, 1145, 1335, 1661, 1806
GsaI	CCCAGC	2 cut(s)	875, 1340
GsuI	CTGGAG	3 cut(s)	1332, 1343, 2126
HaeII	RGCGCY	1 cut(s)	1663
HaeIII	GGCC	3 cut(s)	168, 317, 457
HapII	CCGG	1 cut(s)	2147
HgaI	GACGC	2 cut(s)	319, 809
HhaI	GCGC	6 cut(s)	499, 589, 1146, 1336, 1662, 1807
Hin1I	GRCGYC	1 cut(s)	1660
Hin6I	GCGC	6 cut(s)	497, 587, 1144, 1334, 1660, 1805
HinP1I	GCGC	6 cut(s)	497, 587, 1144, 1334, 1660, 1805
HincII	GTYRAC	2 cut(s)	36, 1015
HindII	GTYRAC	2 cut(s)	36, 1015
HindIII	AAGCTT	2 cut(s)	2219, 2246
HpaI	GTTAAC	1 cut(s)	1015
HpaII	CCGG	1 cut(s)	2147
HphI	GGTGA	2 cut(s)	460, 2402
Hpy166II	GTNNAC	9 cut(s)	36, 161, 894, 1015, 1052, 1289, 1758, 2047, 2095
Hpy188I	TCNGA	3 cut(s)	309, 2182, 2270
Hpy8I	GTNNAC	9 cut(s)	36, 161, 894, 1015, 1052, 1289, 1758, 2047, 2095
HpyAV	CCTTC	3 cut(s)	481, 1573, 1649
HpyCH4IV	ACGT	6 cut(s)	163, 476, 723, 1917, 2031, 2049
HpyF3I	CTNAG	8 cut(s)	72, 485, 681, 971, 1624, 1968, 2079, 2285
HpySE526I	ACGT	6 cut(s)	163, 476, 723, 1917, 2031, 2049
Hsp92I	GRCGYC	1 cut(s)	1660
HspAI	GCGC	6 cut(s)	497, 587, 1144, 1334, 1660, 1805
KasI	GGCGCC	1 cut(s)	1659
Ksp22I	TGATCA	1 cut(s)	378
KspAI	GTTAAC	1 cut(s)	1015
Kzo9I	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
LguI	GCTCTTC	1 cut(s)	1827
LmnI	GCTCC	5 cut(s)	1188, 1313, 1340, 1368, 2107
Lsp1109I	GCAGC	9 cut(s)	63, 74, 80, 91, 758, 1318, 1396, 1532, 1858
LweI	GCATC	4 cut(s)	666, 733, 1430, 1447
MaeI	CTAG	2 cut(s)	657, 2114
MaeII	ACGT	6 cut(s)	163, 476, 723, 1917, 2031, 2049
MaeIII	GTNAC	3 cut(s)	5, 62, 1603
MalI	GATC	6 cut(s)	28, 143, 380, 1098, 1734, 1992
MboI	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
MboII	GAAGA	7 cut(s)	198, 746, 1256, 1721, 1814, 2044, 2377
MflI	RGATCY	2 cut(s)	26, 1096
MhlI	GDGCHC	1 cut(s)	2241
MlsI	TGGCCA	1 cut(s)	168
MluCI	AATT	6 cut(s)	243, 662, 1863, 2132, 2155, 2349
MluNI	TGGCCA	1 cut(s)	168
Mly113I	GGCGCC	1 cut(s)	1660
MlyI	GAGTC	4 cut(s)	1350, 1834, 1875, 2186
MmeI	TCCRAC	2 cut(s)	762, 2003
Mox20I	TGGCCA	1 cut(s)	168
Mph1103I	ATGCAT	3 cut(s)	1024, 1306, 2314
MroXI	GAANNNNTTC	2 cut(s)	665, 1259
MscI	TGGCCA	1 cut(s)	168
MseI	TTAA	8 cut(s)	177, 282, 549, 606, 1014, 1077, 2001, 2277
MslI	CAYNNNNRTG	2 cut(s)	575, 1539
Msp20I	TGGCCA	1 cut(s)	168
MspA1I	CMGCKG	3 cut(s)	76, 416, 1409
MspI	CCGG	1 cut(s)	2147
MspR9I	CCNGG	4 cut(s)	31, 1800, 2013, 2256
Mva1269I	GAATGC	8 cut(s)	589, 802, 1117, 1146, 1191, 1513, 2312, 2399
MvaI	CCWGG	4 cut(s)	31, 1800, 2013, 2256
NarI	GGCGCC	1 cut(s)	1660
NdeII	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
NlaIV	GGNNCC	5 cut(s)	28, 1315, 1661, 1896, 1912
NsiI	ATGCAT	3 cut(s)	1024, 1306, 2314
NspI	RCATGY	2 cut(s)	352, 962
OliI	CACNNNNGTG	1 cut(s)	575
PaeI	GCATGC	2 cut(s)	352, 962
PagI	TCATGA	2 cut(s)	823, 2241
PaqCI	CACCTGC	1 cut(s)	943
PceI	AGGCCT	1 cut(s)	457
PciSI	GCTCTTC	1 cut(s)	1827
PctI	GAATGC	8 cut(s)	589, 802, 1117, 1146, 1191, 1513, 2312, 2399
PdmI	GAANNNNTTC	2 cut(s)	665, 1259
PfeI	GAWTC	8 cut(s)	827, 877, 1394, 1493, 1685, 1781, 1849, 2281
PfoI	TCCNGGA	1 cut(s)	2254
PleI	GAGTC	4 cut(s)	1350, 1834, 1875, 2185
PluTI	GGCGCC	1 cut(s)	1663
PmaCI	CACGTG	2 cut(s)	164, 477
PmlI	CACGTG	2 cut(s)	164, 477
PpsI	GAGTC	4 cut(s)	1350, 1834, 1875, 2185
Ppu21I	YACGTR	4 cut(s)	164, 477, 724, 1918
PsiI	TTATAA	1 cut(s)	1041
Psp6I	CCWGG	4 cut(s)	29, 1798, 2011, 2254
PspCI	CACGTG	2 cut(s)	164, 477
PspFI	CCCAGC	2 cut(s)	871, 1336
PspGI	CCWGG	4 cut(s)	29, 1798, 2011, 2254
PspN4I	GGNNCC	5 cut(s)	28, 1315, 1661, 1896, 1912
PspPI	GGNCC	2 cut(s)	316, 2044
PstI	CTGCAG	2 cut(s)	81, 1876
PsuI	RGATCY	2 cut(s)	26, 1096
PvuII	CAGCTG	2 cut(s)	76, 1409
RsaI	GTAC	6 cut(s)	221, 722, 1920, 1974, 2096, 2145
RsaNI	GTAC	6 cut(s)	220, 721, 1919, 1973, 2095, 2144
RseI	CAYNNNNRTG	2 cut(s)	575, 1539
SapI	GCTCTTC	1 cut(s)	1827
SaqAI	TTAA	8 cut(s)	177, 282, 549, 606, 1014, 1077, 2001, 2277
Sau3AI	GATC	6 cut(s)	26, 141, 378, 1096, 1732, 1990
Sau96I	GGNCC	2 cut(s)	316, 2044
SchI	GAGTC	4 cut(s)	1350, 1834, 1875, 2186
ScrFI	CCNGG	4 cut(s)	31, 1800, 2013, 2256
SduI	GDGCHC	1 cut(s)	2241
SfaNI	GCATC	4 cut(s)	666, 733, 1430, 1447
SfcI	CTRYAG	2 cut(s)	77, 1872
SfoI	GGCGCC	1 cut(s)	1661
SinI	GGWCC	1 cut(s)	2044
SmiMI	CAYNNNNRTG	2 cut(s)	575, 1539
SnaBI	TACGTA	2 cut(s)	724, 1918
SphI	GCATGC	2 cut(s)	352, 962
Sse9I	AATT	6 cut(s)	243, 662, 1863, 2132, 2155, 2349
SseBI	AGGCCT	1 cut(s)	457
SsiI	CCGC	7 cut(s)	226, 414, 1443, 1553, 1749, 1944, 2225
SspDI	GGCGCC	1 cut(s)	1659
SspI	AATATT	4 cut(s)	214, 327, 928, 1163
SspMI	CTAG	2 cut(s)	657, 2114
StuI	AGGCCT	1 cut(s)	457
StyD4I	CCNGG	4 cut(s)	29, 1798, 2011, 2254
StyI	CCWWGG	2 cut(s)	276, 1767
TaiI	ACGT	6 cut(s)	166, 479, 726, 1920, 2034, 2052
TaqI	TCGA	6 cut(s)	121, 268, 330, 669, 1170, 1993
TasI	AATT	6 cut(s)	243, 662, 1863, 2132, 2155, 2349
TatI	WGTACW	1 cut(s)	2094
TauI	GCSGC	1 cut(s)	416
TfiI	GAWTC	8 cut(s)	827, 877, 1394, 1493, 1685, 1781, 1849, 2281
Tru1I	TTAA	8 cut(s)	177, 282, 549, 606, 1014, 1077, 2001, 2277
Tru9I	TTAA	8 cut(s)	177, 282, 549, 606, 1014, 1077, 2001, 2277
TscAI	CASTG	8 cut(s)	57, 364, 388, 527, 919, 1503, 2097, 2218
TseI	GCWGC	9 cut(s)	68, 76, 79, 87, 746, 1331, 1409, 1520, 1871
TspGWI	ACGGA	2 cut(s)	1248, 1990
TspRI	CASTG	8 cut(s)	57, 364, 388, 527, 919, 1503, 2097, 2218
VpaK11BI	GGWCC	1 cut(s)	2044
XapI	RAATTY	2 cut(s)	243, 1863
XceI	RCATGY	2 cut(s)	352, 962
XcmI	CCANNNNNNNNNTGG	1 cut(s)	401
XmnI	GAANNNNTTC	2 cut(s)	665, 1259
XspI	CTAG	2 cut(s)	657, 2114
Zsp2I	ATGCAT	3 cut(s)	1024, 1306, 2314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.