Rh7DG341100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004093
Physical Location & Seq
Forward (+)
44982134 .. 44985522
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG341100.1

Sequence Viewer

Length: 1353 bp
ATGATCGCTTCCGGCTTCCACCCACGTGGCCATATCCTCAACCGTTTGATCGACGTCTACTGCAAATCTTCAAATCTTCGCTACGCACTCCACCTGTTCGACCAAATTCCCAACCCGGACATCGTCGCCAGAACGACTCTAGTCTCGGCATATTCCGGCGTCGGGGACGTCACGCTGGCCCGGAGAGTCTTCAGTGAAACTCCGCTCAGCATGAGAGACACTGTTAGCTACAATGCCATGATCGCAGGCTACTCTCATAACAGGGACGGCAATGCCGCCGTTGAGCTGTTTCGTGACATGAGGCGAGCCGGTTTTCGGCCCGATGAGTTTACCTTCACCAGCGTGCTGAGCTCTCTGGCGCTTATAGTGGAGGAGGAGAAGCAATGCGAGCAGCTGCATTGTGCAATTGTTAAGTCTGGAGCATTGCTGGCCACTTCGGTGTCAAATGCGTTGGTGTCTGTGTATGTTAAGTGTGCTTCTTCGCCGTTGGTGTCGGCGTCCTCGGTGGTGGTGGCTGCAGCGAGGAAGGTGTTTGATGAAATGCCTAAGAGAGATGAGCTGTCGTGGACGACGATGATTACTGGCTATGTGAGGAATGAGGACCTTGATGGAGCTCGGCAGCTGCTTGATGGGATGGATGAAAAATTGCAAGTGGCGTGGAATGCCATGATTTCGGGTTATGTGCATCACGGTTTTTGTCATGAAGCATTGAAATTGTTTAGGGAAATGTATTTAATGGGGATTCGCCAGGATGAGTTTACGTACACGAGTGTTATCAGTGCTTGTGCTAACAAGGGCTTATTTAAGCATGGGAAGCAGCTGCATGCTTACATACTAAGATCTGAAGCGAAACCGACTCTGGACTTTTCTTTATCTGTGAATAATGCATTGGTGACACTATACTTTAGGTGCGGTAAAATTGATGAAGCACGATATATTTTTAATAATATGTCTGTCAGAGATCTTGTTTCTTGGAATGCGATATTGTCAGGGTATGTGAGTGCAGGGCGCATTCAGGAAGCAAAGTCCTTTTTTGAGGAAATGCCGGAAAGGAGTGGTCTAACGTGGACTGTAATAATATCTGGTTTAGCTCAAAATGGATGTGGGGAAGAAGCTATGAAGCTATTTAACCAAATGAGATTAGATGGTTTTAAACCCTGTGATTATGCATTTGCTGGAGCTATTACGTCTTGTGCTGCTCTTGGGGCATTGGAGCACGGACGCCAGCTCCATGCTCAACTAATATGCTTGGGGCATGATTCAAGCCTTTCAGTCGGAAATTCACTTATTACGATTGAAAGCTACTTGGAACTCTATTGGAATATATCACCTACCTTAATAACAGTGAACTAG

Protein Analysis

450

Amino Acids

49.92

Weight (kDa)

6.36

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 120 7.3e-15 PPR repeat family
PPR PF01535 75 - 105 1.2e-07 PPR repeat
PPR_2 PF13041 218 - 264 1.8e-12 PPR repeat family
PPR PF01535 218 - 247 8.4e-09 PPR repeat
PPR_2 PF13041 321 - 351 1e-06 PPR repeat family
PPR PF01535 323 - 351 2.2e-07 PPR repeat
PPR_2 PF13041 355 - 398 2.5e-07 PPR repeat family
PPR PF01535 355 - 384 4.4e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 57, 171
AccBSI CCGCTC 1 cut(s) 205
AccI GTMKAC 1 cut(s) 57
AciI CCGC 3 cut(s) 203, 276, 912
AcoI YGGCCR 2 cut(s) 28, 429
AcsI RAATTY 2 cut(s) 105, 1279
AcuI CTGAAG 2 cut(s) 175, 864
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 2 cut(s) 162, 315
AgsI TTSAA 4 cut(s) 72, 712, 1263, 1298
AjnI CCWGG 1 cut(s) 747
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
AleI CACNNNNGTG 3 cut(s) 24, 341, 437
Alw21I GWGCWC 3 cut(s) 353, 616, 1218
Alw26I GTCTC 2 cut(s) 148, 210
AoxI GGCC 4 cut(s) 28, 177, 317, 429
ApeKI GCWGC 9 cut(s) 391, 394, 515, 518, 619, 622, 817, 820, 1196
ApoI RAATTY 2 cut(s) 105, 1279
AspLEI GCGC 2 cut(s) 361, 1011
AspS9I GGNCC 3 cut(s) 178, 318, 601
AsuC2I CCSGG 2 cut(s) 116, 181
AsuHPI GGTGA 3 cut(s) 328, 904, 1320
AvaII GGWCC 1 cut(s) 601
BalI TGGCCA 2 cut(s) 30, 431
BanII GRGCYC 2 cut(s) 353, 616
BauI CACGAG 1 cut(s) 766
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 3 cut(s) 353, 616, 1218
BbvI GCAGC 9 cut(s) 381, 403, 502, 530, 609, 631, 807, 829, 1183
BccI CCATC 4 cut(s) 602, 623, 628, 1139
BceAI ACGGC 3 cut(s) 263, 283, 469
BciT130I CCWGG 1 cut(s) 749
BcnI CCSGG 2 cut(s) 116, 181
BcoDI GTCTC 2 cut(s) 148, 210
BfaI CTAG 2 cut(s) 140, 1351
BfmI CTRYAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 362
BglII AGATCT 2 cut(s) 839, 961
BlpI GCTNAGC 2 cut(s) 206, 347
Bme1390I CCNGG 3 cut(s) 116, 181, 749
Bme18I GGWCC 1 cut(s) 601
BmgT120I GGNCC 3 cut(s) 178, 318, 601
BmrFI CCNGG 3 cut(s) 116, 181, 749
BmsI GCATC 1 cut(s) 694
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 181
BpmI CTGGAG 2 cut(s) 438, 1197
Bpu1102I GCTNAGC 2 cut(s) 206, 347
BpuMI CCSGG 2 cut(s) 116, 181
BsaAI YACGTR 2 cut(s) 26, 762
BsaHI GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
BsaJI CCNNGG 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 1212, 1242
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 315
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 1 cut(s) 586
Bse3DI GCAATG 3 cut(s) 277, 389, 422
BseBI CCWGG 1 cut(s) 749
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 4 cut(s) 639, 643, 757, 1106
BseLI CCNNNNNNNGG 2 cut(s) 162, 315
BseMI GCAATG 3 cut(s) 277, 389, 422
BseMII CTCAG 2 cut(s) 220, 338
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 2 cut(s) 386, 389
BseXI GCAGC 9 cut(s) 381, 403, 502, 530, 609, 631, 807, 829, 1183
BsgI GTGCAG 1 cut(s) 1023
BshFI GGCC 4 cut(s) 30, 179, 319, 431
BsiHKAI GWGCWC 3 cut(s) 353, 616, 1218
BsiSI CCGG 6 cut(s) 12, 116, 156, 181, 309, 1046
BslFI GGGAC 2 cut(s) 179, 278
BslI CCNNNNNNNGG 2 cut(s) 162, 315
BsmAI GTCTC 2 cut(s) 148, 210
BsmFI GGGAC 2 cut(s) 179, 278
BsmI GAATGC 3 cut(s) 667, 982, 1011
BsnI GGCC 4 cut(s) 30, 179, 319, 431
Bsp1286I GDGCHC 3 cut(s) 353, 616, 1218
Bsp143I GATC 5 cut(s) 3, 48, 240, 839, 961
Bsp1720I GCTNAGC 2 cut(s) 206, 347
BspACI CCGC 3 cut(s) 203, 276, 912
BspANI GGCC 4 cut(s) 30, 179, 319, 431
BspCNI CTCAG 2 cut(s) 219, 339
BspHI TCATGA 1 cut(s) 700
BspMAI CTGCAG 1 cut(s) 520
BsrBI CCGCTC 1 cut(s) 205
BsrDI GCAATG 3 cut(s) 277, 389, 422
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 1 cut(s) 586
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 5 cut(s) 3, 48, 240, 839, 961
BssNI GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
BssSI CACGAG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 766
Bst2UI CCWGG 1 cut(s) 749
Bst4CI ACNGT 5 cut(s) 44, 223, 692, 1072, 1345
BstACI GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
BstBAI YACGTR 2 cut(s) 26, 762
BstC8I GCNNGC 8 cut(s) 177, 247, 306, 344, 389, 429, 825, 1226
BstDEI CTNAG 4 cut(s) 206, 347, 546, 836
BstF5I GGATG 4 cut(s) 639, 643, 757, 1106
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 2 cut(s) 361, 1011
BstKTI GATC 5 cut(s) 6, 51, 243, 842, 964
BstMAI GTCTC 2 cut(s) 148, 210
BstMBI GATC 5 cut(s) 3, 48, 240, 839, 961
BstMWI GCNNNNNNNGC 7 cut(s) 242, 348, 388, 428, 662, 814, 1205
BstNI CCWGG 1 cut(s) 749
BstNSI RCATGY 1 cut(s) 827
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 114, 179, 747
BstSFI CTRYAG 1 cut(s) 516
BstSNI TACGTA 1 cut(s) 762
BstV1I GCAGC 9 cut(s) 381, 403, 502, 530, 609, 631, 807, 829, 1183
BstV2I GAAGAC 1 cut(s) 181
BstX2I RGATCY 2 cut(s) 839, 961
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 2 cut(s) 839, 961
BsuRI GGCC 4 cut(s) 30, 179, 319, 431
BtsCI GGATG 4 cut(s) 639, 643, 757, 1106
BtsIMutI CAGTG 4 cut(s) 199, 219, 784, 1350
Cac8I GCNNGC 8 cut(s) 177, 247, 306, 344, 389, 429, 825, 1226
CciI TCATGA 1 cut(s) 700
CfoI GCGC 2 cut(s) 361, 1011
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 178, 318, 601
CseI GACGC 3 cut(s) 148, 486, 1230
Csp6I GTAC 1 cut(s) 763
CspCI CAANNNNNGTGG 2 cut(s) 638, 673
CviAII CATG 9 cut(s) 211, 238, 298, 667, 701, 809, 824, 1232, 1256
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 4 cut(s) 206, 347, 546, 836
DpnI GATC 5 cut(s) 5, 50, 242, 841, 963
DpnII GATC 5 cut(s) 3, 48, 240, 839, 961
DraI TTTAAA 1 cut(s) 1153
EaeI YGGCCR 2 cut(s) 28, 429
Ecl136II GAGCTC 2 cut(s) 351, 614
Eco105I TACGTA 1 cut(s) 762
Eco24I GRGCYC 2 cut(s) 353, 616
Eco47I GGWCC 1 cut(s) 601
Eco53kI GAGCTC 2 cut(s) 351, 614
Eco57I CTGAAG 2 cut(s) 175, 864
Eco72I CACGTG 1 cut(s) 26
EcoICRI GAGCTC 2 cut(s) 351, 614
EcoO109I RGGNCCY 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 747
EcoT22I ATGCAT 2 cut(s) 889, 1171
EcoT38I GRGCYC 2 cut(s) 353, 616
FaeI CATG 9 cut(s) 214, 241, 301, 670, 704, 812, 827, 1235, 1259
FaqI GGGAC 2 cut(s) 179, 278
FatI CATG 9 cut(s) 210, 237, 297, 666, 700, 808, 823, 1231, 1255
FblI GTMKAC 1 cut(s) 57
FokI GGATG 4 cut(s) 646, 650, 764, 1113
FriOI GRGCYC 2 cut(s) 353, 616
FspBI CTAG 2 cut(s) 140, 1351
GlaI GCGC 2 cut(s) 360, 1010
GsuI CTGGAG 2 cut(s) 438, 1197
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 4 cut(s) 30, 179, 319, 431
HapII CCGG 6 cut(s) 12, 116, 156, 181, 309, 1046
HgaI GACGC 3 cut(s) 148, 486, 1230
HhaI GCGC 2 cut(s) 361, 1011
Hin1I GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
Hin1II CATG 9 cut(s) 214, 241, 301, 670, 704, 812, 827, 1235, 1259
Hin6I GCGC 2 cut(s) 359, 1009
HinP1I GCGC 2 cut(s) 359, 1009
HinfI GANTC 5 cut(s) 136, 186, 742, 856, 1259
HpaII CCGG 6 cut(s) 12, 116, 156, 181, 309, 1046
HphI GGTGA 3 cut(s) 328, 904, 1320
Hpy166II GTNNAC 7 cut(s) 58, 330, 567, 759, 765, 1068, 1348
Hpy188I TCNGA 3 cut(s) 844, 959, 1277
Hpy188III TCNNGA 5 cut(s) 293, 417, 701, 860, 1016
Hpy8I GTNNAC 7 cut(s) 58, 330, 567, 759, 765, 1068, 1348
Hpy99I CGWCG 4 cut(s) 56, 128, 164, 574
HpyAV CCTTC 2 cut(s) 343, 520
HpyCH4III ACNGT 5 cut(s) 44, 223, 692, 1072, 1345
HpyCH4IV ACGT 6 cut(s) 25, 54, 168, 761, 1064, 1187
HpyF10VI GCNNNNNNNGC 7 cut(s) 242, 348, 388, 428, 662, 814, 1205
HpyF3I CTNAG 4 cut(s) 206, 347, 546, 836
HpySE526I ACGT 6 cut(s) 25, 54, 168, 761, 1064, 1187
Hsp92I GRCGYC 5 cut(s) 54, 159, 168, 497, 1222
Hsp92II CATG 9 cut(s) 214, 241, 301, 670, 704, 812, 827, 1235, 1259
HspAI GCGC 2 cut(s) 359, 1009
Kzo9I GATC 5 cut(s) 3, 48, 240, 839, 961
LmnI GCTCC 5 cut(s) 419, 611, 1178, 1213, 1233
Lsp1109I GCAGC 9 cut(s) 381, 403, 502, 530, 609, 631, 807, 829, 1183
LweI GCATC 1 cut(s) 694
MaeI CTAG 2 cut(s) 140, 1351
MaeII ACGT 6 cut(s) 25, 54, 168, 761, 1064, 1187
MaeIII GTNAC 3 cut(s) 169, 293, 892
MalI GATC 5 cut(s) 5, 50, 242, 841, 963
MbiI CCGCTC 1 cut(s) 205
MboI GATC 5 cut(s) 3, 48, 240, 839, 961
MboII GAAGA 5 cut(s) 60, 68, 181, 471, 1121
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 839, 961
MhlI GDGCHC 3 cut(s) 353, 616, 1218
MlsI TGGCCA 2 cut(s) 30, 431
MluCI AATT 6 cut(s) 105, 405, 644, 713, 918, 1279
MluNI TGGCCA 2 cut(s) 30, 431
MlyI GAGTC 3 cut(s) 130, 195, 850
MmeI TCCRAC 1 cut(s) 1255
MnlI CCTC 9 cut(s) 47, 294, 364, 367, 511, 516, 585, 592, 1030
Mox20I TGGCCA 2 cut(s) 30, 431
Mph1103I ATGCAT 2 cut(s) 889, 1171
MscI TGGCCA 2 cut(s) 30, 431
MseI TTAA 8 cut(s) 411, 468, 734, 804, 942, 1128, 1152, 1337
MslI CAYNNNNRTG 3 cut(s) 24, 341, 437
Msp20I TGGCCA 2 cut(s) 30, 431
MspA1I CMGCKG 3 cut(s) 394, 622, 820
MspI CCGG 6 cut(s) 12, 116, 156, 181, 309, 1046
MspR9I CCNGG 3 cut(s) 116, 181, 749
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 3 cut(s) 667, 982, 1011
MvaI CCWGG 1 cut(s) 749
MwoI GCNNNNNNNGC 7 cut(s) 242, 348, 388, 428, 662, 814, 1205
NciI CCSGG 2 cut(s) 116, 181
NdeII GATC 5 cut(s) 3, 48, 240, 839, 961
NlaIII CATG 9 cut(s) 214, 241, 301, 670, 704, 812, 827, 1235, 1259
NmeAIII GCCGAG 2 cut(s) 125, 595
NmuCI GTSAC 3 cut(s) 169, 293, 892
NsiI ATGCAT 2 cut(s) 889, 1171
NspI RCATGY 1 cut(s) 827
OliI CACNNNNGTG 3 cut(s) 24, 341, 437
PaeI GCATGC 1 cut(s) 827
PagI TCATGA 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PctI GAATGC 3 cut(s) 667, 982, 1011
PfeI GAWTC 2 cut(s) 742, 1259
PflFI GACNNNGTC 1 cut(s) 122
PleI GAGTC 3 cut(s) 130, 194, 850
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 3 cut(s) 130, 194, 850
Ppu21I YACGTR 2 cut(s) 26, 762
PpuMI RGGWCCY 1 cut(s) 601
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 353, 616
Psp5II RGGWCCY 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 747
PspCI CACGTG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 747
PspPI GGNCC 3 cut(s) 178, 318, 601
PspPPI RGGWCCY 1 cut(s) 601
PstI CTGCAG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 839, 961
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 3 cut(s) 394, 622, 820
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
RseI CAYNNNNRTG 3 cut(s) 24, 341, 437
SacI GAGCTC 2 cut(s) 353, 616
SaqAI TTAA 8 cut(s) 411, 468, 734, 804, 942, 1128, 1152, 1337
Sau3AI GATC 5 cut(s) 3, 48, 240, 839, 961
Sau96I GGNCC 3 cut(s) 178, 318, 601
SchI GAGTC 3 cut(s) 130, 195, 850
ScrFI CCNGG 3 cut(s) 116, 181, 749
SduI GDGCHC 3 cut(s) 353, 616, 1218
SfaNI GCATC 1 cut(s) 694
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 601
SmiMI CAYNNNNRTG 3 cut(s) 24, 341, 437
SnaBI TACGTA 1 cut(s) 762
SphI GCATGC 1 cut(s) 827
Sse9I AATT 6 cut(s) 105, 405, 644, 713, 918, 1279
SsiI CCGC 3 cut(s) 203, 276, 912
SspMI CTAG 2 cut(s) 140, 1351
SstI GAGCTC 2 cut(s) 353, 616
StyD4I CCNGG 3 cut(s) 114, 179, 747
TaaI ACNGT 5 cut(s) 44, 223, 692, 1072, 1345
TaiI ACGT 6 cut(s) 28, 57, 171, 764, 1067, 1190
TaqI TCGA 2 cut(s) 51, 99
TasI AATT 6 cut(s) 105, 405, 644, 713, 918, 1279
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 742, 1259
Tru1I TTAA 8 cut(s) 411, 468, 734, 804, 942, 1128, 1152, 1337
Tru9I TTAA 8 cut(s) 411, 468, 734, 804, 942, 1128, 1152, 1337
TscAI CASTG 4 cut(s) 199, 226, 784, 1350
TseFI GTSAC 3 cut(s) 169, 293, 892
TseI GCWGC 9 cut(s) 391, 394, 515, 518, 619, 622, 817, 820, 1196
Tsp45I GTSAC 3 cut(s) 169, 293, 892
TspDTI ATGAA 5 cut(s) 552, 654, 717, 939, 1133
TspGWI ACGGA 1 cut(s) 1233
TspRI CASTG 4 cut(s) 199, 226, 784, 1350
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 601
XapI RAATTY 2 cut(s) 105, 1279
XceI RCATGY 1 cut(s) 827
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 2 cut(s) 140, 1351
ZraI GACGTC 2 cut(s) 55, 169
Zsp2I ATGCAT 2 cut(s) 889, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.