Rw0G004030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00154
OG0004093
Physical Location & Seq
Reverse (-)
54010 .. 56878
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004030.1

Sequence Viewer

Length: 2334 bp
ATGTATTGCCCACCACCAAACCTGTCTCCGAATCGTCCTCACCGTCTTACGGTGGCGGCTAGCCGTTACGCCGCCGGACTGCAGCTATGCTGCCCCCAACGCCCCACCTCCTACTCTTTAGCCCGAACCATCTACTCCCACATGATCGCTTCCGGCTTCAACCCACGTGGTCATATCCTCAACCGTTTGATTGACGTCTATAGCAAATGTTCCAATCTCCGTTACGCACTCCACCTGTTCGACGCAATTCCCAACCCGGACATCATCGCCAGAACAACTCTGGTCTCGGTGTATTCTGGCGTCGGGGACGTGGTGCTGGCCCGGAGAGTGTTCAGTGAAACTCTGCTCAGCATGAGAGACACTGTCAGCTACAATGCCATGATCGCAGGCTACTCTCATAACAGGGACGGCAATGCCGCCGTTGAGCTGTTTCGTGACATGAGGCGAGGCGGTTTTCGGCCCGATGAGTTTACCTTCACCAGTGTGCTGAGTTCTCTGGCGCTTATAGTGGAGGAGGAGAAGCAATGCCAGCAGCTGCATTGTGCAATTGTTAAGTCTGGAGCATTGCTGGCAACTTCGGTGTCGAATGCTTTGGTGTCTGTGTATGTTAAGTGTGCTTCTTCGCCGTTGGTGTCGAAGGTGTTTGATGAAATGCCAAAGAGAGATGAGCTGTCGTGGACGACGATGATTACTGGCTATGTGAGGAATGAGGACCTTGATGGAGCTCGGCAGCTGCTTGATGGGATGGATGAAAAATTGGAAGTGGCATGGAATGCGATGATTTCGGGTTACGTGCATCATGGTTTTTGTGATGAAGCATTGAAGTTGTTTAGGGAAATGCATTTAATGGGGATTCGCAGTGCTTGTGCTAACAATGGGTTATTTAAGCATGGGAAGCAGTTGCATGCTTACATACTAAGATCTGAAGTGAAACCGACTGTGGACTTTTCTTTATCTGTGAATAATGCATTGGTTACGCTATACTTTAGGTGCGGTAAAATTGATGAAGCACGATATATTTTTAATAATATGTCCGTCAGAGATCTTGTTTCTTGGAATGCAATATTGTCAGGGTATGTGAGTGGAGGGCGCATCCAGGAAGCAAAATCCTTTTTTGAGGAAATGCCGGAAAGGAGTGGTCTAACTTGGACTGTAATAATATCAGGTTTAGCTCAAAATGGATGTGGGGAAGAAGCTATGAAGCTATTTAACCAAATGAGACTAGATGGTTTTAAACCCTGTGATTATGCATTTGCTGGAGCTATTACGTCTTGTGCTGCTCTGGGGGCGTTGGAGCATGGACGCCAGCTCCATGCTCAACTAATATGCTTGGGGCATGATTCAAACCTTTTAGTTGGAAATTCACTTATCACAATGTATGCCAGATGTGGGGTTGTAGACGATGCTGACTGTGTGTTCCTTACAATGCCTTATATTGATTCCGTGTCGTGGAATGCTATGATCGCGGCTCTGGCACAACATGGATATGGTGTCCAAGCAATTAGACTTTTCAAACAGATGTTGGGGGAAGATATAATGCTAGATAGAATAACTTTCCTCACTATTCTGTCAGCTTGTAGTCATGCTGGTTTAGTTAAAGAAGGACGCCAATATTTTCGTTTAATGCATGACTCTTATGGTATTGCCCCTGGTGAAGACCATTATGCTCGTATGATTGATTTGTTGTGCCGATCTGGGGAGTTGACAGAGGCCAATGACTTAATCAAATCAATGCCTTTTGAACCAGGTGCACCAATTTGGGAGGCTATTCTAGCTGGTTGCCGGACTCATGGGAATATGGATTTAGGTATTCAAGCTGCAGAACGACTCTTTGAGCTGGTGCCCCAGCATGATGGAACCTATGTACTTTTGTCGAACTTGTATGCTGCTTTAGGCCGTTGGGATAATGTGGCTAAGGTGCGAAAACTAATGAGGGAGCGAGGGGTTAAGAAAGAACCTGGTTGTAGTTGGATTGACGTTGAAAATATGGTCCATGTCTTCTTGGTTGGTGATACTTATCTAGAGCAACTGGTTCTTAAAATGAGGAAGTTAGGATATGCTCATGACACAAAGTTTGCGTTGCATGATATGGAGTCTGAGCATAAAGAGAATTCCTTGTCTACTCACAGTGAGAAGCTTGCAGTTGCATTTGGGATCATGAAGCTTCCACTTGGAGCCACGATCAGAGTTTTTAAGAATCTTAGGATCTGTGGGGATTGCCATAACGCATTTAAGTTTATGTCAAGAGTGGTTGGGAGAGAGATAGTTGTGAGAGACGCCAAGAGGTTTCATCATTTTAGGAACGGCGAATGCTCTTGTGGAAATTACTGGTAG

Protein Analysis

777

Amino Acids

86.87

Weight (kDa)

7.54

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 119 - 167 1.7e-14 PPR repeat family
PPR PF01535 122 - 152 5.8e-07 PPR repeat
PPR PF01535 255 - 285 1.1e-09 PPR repeat
PPR_2 PF13041 255 - 285 5.8e-06 PPR repeat family
PPR PF01535 350 - 378 1.7e-06 PPR repeat
PPR PF01535 382 - 411 8.5e-08 PPR repeat
PPR_2 PF13041 382 - 425 5.1e-07 PPR repeat family
PPR_2 PF13041 480 - 527 1.7e-08 PPR repeat family
PPR PF01535 482 - 511 8.5e-06 PPR repeat
E_motif PF20431 597 - 659 2.7e-22 E motif
DYW_deaminase PF14432 685 - 777 1.7e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 1840
AccI GTMKAC 2 cut(s) 1398, 2120
AccII CGCG 1 cut(s) 1466
AciI CCGC 6 cut(s) 56, 72, 417, 450, 993, 1466
AclWI GGATC 2 cut(s) 2162, 2213
AcsI RAATTY 2 cut(s) 1360, 2110
AcuI CTGAAG 1 cut(s) 945
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
AfaI GTAC 1 cut(s) 1866
AfiI CCNNNNNNNGG 4 cut(s) 49, 1389, 1449, 1696
AgsI TTSAA 7 cut(s) 160, 823, 1344, 1513, 1742, 1814, 1982
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 4 cut(s) 1095, 1648, 1744, 1957
AjuI GAANNNNNNNTTGG 2 cut(s) 953, 985
AleI CACNNNNGTG 1 cut(s) 482
Alw21I GWGCWC 2 cut(s) 727, 1753
Alw26I GTCTC 5 cut(s) 30, 289, 351, 1213, 2268
Alw44I GTGCAC 1 cut(s) 1749
AlwI GGATC 2 cut(s) 2162, 2213
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 4 cut(s) 318, 458, 1710, 1894
ApaLI GTGCAC 1 cut(s) 1749
ApeKI GCWGC 9 cut(s) 82, 90, 532, 535, 730, 733, 1277, 1817, 1886
ApoI RAATTY 2 cut(s) 1360, 2110
ArsI GACNNNNNNTTYG 2 cut(s) 2057, 2089
AspLEI GCGC 2 cut(s) 502, 1092
AspS9I GGNCC 4 cut(s) 319, 459, 712, 1990
AsuC2I CCSGG 2 cut(s) 257, 322
AsuHPI GGTGA 4 cut(s) 32, 469, 1664, 2021
AsuNHI GCTAGC 1 cut(s) 59
AvaII GGWCC 2 cut(s) 712, 1990
BaeGI GKGCMC 2 cut(s) 1753, 1845
BanI GGYRCC 1 cut(s) 1840
BanII GRGCYC 1 cut(s) 727
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 1662, 1990
Bbv12I GWGCWC 2 cut(s) 727, 1753
BbvI GCAGC 9 cut(s) 77, 94, 522, 544, 720, 742, 1264, 1804, 1873
BccI CCATC 6 cut(s) 137, 713, 734, 739, 1220, 1847
BceAI ACGGC 6 cut(s) 48, 404, 424, 610, 1881, 2320
BciT130I CCWGG 4 cut(s) 1097, 1650, 1746, 1959
BcnI CCSGG 2 cut(s) 257, 322
BcoDI GTCTC 5 cut(s) 30, 289, 351, 1213, 2268
BfaI CTAG 5 cut(s) 60, 1223, 1541, 1772, 2021
BfmI CTRYAG 3 cut(s) 80, 199, 1818
BfoI RGCGCY 1 cut(s) 503
BglII AGATCT 2 cut(s) 920, 1042
BlpI GCTNAGC 1 cut(s) 347
Bme1390I CCNGG 6 cut(s) 257, 322, 1097, 1650, 1746, 1959
Bme18I GGWCC 2 cut(s) 712, 1990
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 4 cut(s) 319, 459, 712, 1990
BmiI GGNNCC 3 cut(s) 1842, 1858, 2176
BmrFI CCNGG 6 cut(s) 257, 322, 1097, 1650, 1746, 1959
BmsI GCATC 3 cut(s) 805, 1101, 1393
BmtI GCTAGC 1 cut(s) 63
BpiI GAAGAC 2 cut(s) 1662, 1990
BpmI CTGGAG 2 cut(s) 579, 1278
Bpu10I CCTNAGC 1 cut(s) 1914
Bpu1102I GCTNAGC 1 cut(s) 347
BpuMI CCSGG 2 cut(s) 257, 322
BsaAI YACGTR 2 cut(s) 167, 793
BsaBI GATNNNNATC 1 cut(s) 2016
BsaHI GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 1648
BsaXI ACNNNNNCTCC 2 cut(s) 1293, 1323
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 1389, 1449, 1696
Bse1I ACTGG 4 cut(s) 480, 697, 2034, 2333
Bse3DI GCAATG 3 cut(s) 418, 530, 563
Bse8I GATNNNNATC 1 cut(s) 2016
BseBI CCWGG 4 cut(s) 1097, 1650, 1746, 1959
BseDI CCNNGG 1 cut(s) 1648
BseGI GGATG 4 cut(s) 750, 754, 1092, 1187
BseJI GATNNNNATC 1 cut(s) 2016
BseLI CCNNNNNNNGG 4 cut(s) 49, 1389, 1449, 1696
BseMI GCAATG 3 cut(s) 418, 530, 563
BseMII CTCAG 3 cut(s) 361, 479, 2088
BseNI ACTGG 4 cut(s) 480, 697, 2034, 2333
BseRI GAGGAG 2 cut(s) 527, 530
BseSI GKGCMC 2 cut(s) 1753, 1845
BseXI GCAGC 9 cut(s) 77, 94, 522, 544, 720, 742, 1264, 1804, 1873
BseYI CCCAGC 1 cut(s) 1845
Bsh1236I CGCG 1 cut(s) 1466
BshFI GGCC 4 cut(s) 320, 460, 1712, 1896
BshNI GGYRCC 1 cut(s) 1840
BsiHKAI GWGCWC 2 cut(s) 727, 1753
BsiSI CCGG 6 cut(s) 75, 153, 257, 322, 1127, 1783
BslFI GGGAC 2 cut(s) 320, 419
BslI CCNNNNNNNGG 4 cut(s) 49, 1389, 1449, 1696
BsmAI GTCTC 5 cut(s) 30, 289, 351, 1213, 2268
BsmBI CGTCTC 1 cut(s) 2268
BsmFI GGGAC 2 cut(s) 320, 419
BsmI GAATGC 5 cut(s) 592, 778, 1063, 1459, 2315
BsnI GGCC 4 cut(s) 320, 460, 1712, 1896
Bso31I GGTCTC 1 cut(s) 289
Bsp1286I GDGCHC 3 cut(s) 727, 1753, 1845
Bsp143I GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 6 cut(s) 56, 72, 417, 450, 993, 1466
BspANI GGCC 4 cut(s) 320, 460, 1712, 1896
BspCNI CTCAG 3 cut(s) 360, 480, 2089
BspFNI CGCG 1 cut(s) 1466
BspHI TCATGA 2 cut(s) 2062, 2157
BspLI GGNNCC 3 cut(s) 1842, 1858, 2176
BspMAI CTGCAG 2 cut(s) 84, 1822
BspOI GCTAGC 1 cut(s) 63
BspPI GGATC 2 cut(s) 2162, 2213
BspT107I GGYRCC 1 cut(s) 1840
BspTNI GGTCTC 1 cut(s) 289
BsrDI GCAATG 3 cut(s) 418, 530, 563
BsrI ACTGG 4 cut(s) 480, 697, 2034, 2333
BssECI CCNNGG 1 cut(s) 1648
BssMI GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
BssNI GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
Bst2UI CCWGG 4 cut(s) 1097, 1650, 1746, 1959
Bst4CI ACNGT 8 cut(s) 44, 52, 185, 364, 940, 1153, 1412, 2129
BstACI GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBAI YACGTR 2 cut(s) 167, 793
BstC8I GCNNGC 8 cut(s) 61, 318, 388, 530, 570, 906, 1307, 2139
BstDEI CTNAG 6 cut(s) 347, 488, 917, 1914, 2097, 2201
BstF5I GGATG 4 cut(s) 750, 754, 1092, 1187
BstFNI CGCG 1 cut(s) 1466
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 2 cut(s) 502, 1092
BstKTI GATC 9 cut(s) 147, 384, 923, 1045, 1464, 1694, 2157, 2184, 2208
BstMAI GTCTC 5 cut(s) 30, 289, 351, 1213, 2268
BstMBI GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
BstNI CCWGG 4 cut(s) 1097, 1650, 1746, 1959
BstNSI RCATGY 1 cut(s) 908
BstSCI CCNGG 6 cut(s) 255, 320, 1095, 1648, 1744, 1957
BstSFI CTRYAG 3 cut(s) 80, 199, 1818
BstSLI GKGCMC 2 cut(s) 1753, 1845
BstUI CGCG 1 cut(s) 1466
BstV1I GCAGC 9 cut(s) 77, 94, 522, 544, 720, 742, 1264, 1804, 1873
BstV2I GAAGAC 2 cut(s) 1662, 1990
BstX2I RGATCY 3 cut(s) 920, 1042, 2205
BstXI CCANNNNNNTGG 1 cut(s) 1853
BstYI RGATCY 3 cut(s) 920, 1042, 2205
BsuRI GGCC 4 cut(s) 320, 460, 1712, 1896
BtgZI GCGATG 2 cut(s) 250, 791
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 4 cut(s) 750, 754, 1092, 1187
BtsI GCAGTG 1 cut(s) 865
BtsIMutI CAGTG 5 cut(s) 340, 360, 487, 865, 2134
Cac8I GCNNGC 8 cut(s) 61, 318, 388, 530, 570, 906, 1307, 2139
CaiI CAGNNNCTG 1 cut(s) 535
CciI TCATGA 2 cut(s) 2062, 2157
CfoI GCGC 2 cut(s) 502, 1092
Cfr13I GGNCC 4 cut(s) 319, 459, 712, 1990
CseI GACGC 5 cut(s) 251, 289, 1311, 1614, 2285
CsiI ACCWGGT 2 cut(s) 1744, 1957
Csp6I GTAC 1 cut(s) 1865
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviQI GTAC 1 cut(s) 1865
DdeI CTNAG 6 cut(s) 347, 488, 917, 1914, 2097, 2201
DpnI GATC 9 cut(s) 146, 383, 922, 1044, 1463, 1693, 2156, 2183, 2207
DpnII GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
DraI TTTAAA 1 cut(s) 1234
Ecl136II GAGCTC 1 cut(s) 725
Eco24I GRGCYC 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 712, 1990
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 1 cut(s) 945
Eco72I CACGTG 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 712
EcoRI GAATTC 1 cut(s) 2110
EcoRII CCWGG 4 cut(s) 1095, 1648, 1744, 1957
EcoT22I ATGCAT 4 cut(s) 843, 970, 1252, 1629
EcoT38I GRGCYC 1 cut(s) 727
Esp3I CGTCTC 1 cut(s) 2268
FaqI GGGAC 2 cut(s) 320, 419
FblI GTMKAC 2 cut(s) 1398, 2120
FokI GGATG 4 cut(s) 757, 761, 1079, 1194
FriOI GRGCYC 1 cut(s) 727
FspBI CTAG 5 cut(s) 60, 1223, 1541, 1772, 2021
GlaI GCGC 2 cut(s) 501, 1091
GsaI CCCAGC 1 cut(s) 1849
GsuI CTGGAG 2 cut(s) 579, 1278
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 4 cut(s) 320, 460, 1712, 1896
HapII CCGG 6 cut(s) 75, 153, 257, 322, 1127, 1783
HgaI GACGC 5 cut(s) 251, 289, 1311, 1614, 2285
HhaI GCGC 2 cut(s) 502, 1092
Hin1I GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
Hin6I GCGC 2 cut(s) 500, 1090
HinP1I GCGC 2 cut(s) 500, 1090
HincII GTYRAC 1 cut(s) 1704
HindII GTYRAC 1 cut(s) 1704
HindIII AAGCTT 2 cut(s) 2135, 2162
HinfI GANTC 9 cut(s) 31, 853, 1340, 1439, 1631, 1786, 1827, 2093, 2197
HpaII CCGG 6 cut(s) 75, 153, 257, 322, 1127, 1783
HphI GGTGA 4 cut(s) 32, 469, 1664, 2021
Hpy166II GTNNAC 7 cut(s) 471, 678, 943, 1399, 1704, 1751, 2121
Hpy188I TCNGA 5 cut(s) 30, 925, 1040, 2098, 2186
Hpy188III TCNNGA 6 cut(s) 434, 558, 2021, 2063, 2158, 2244
Hpy8I GTNNAC 7 cut(s) 471, 678, 943, 1399, 1704, 1751, 2121
Hpy99I CGWCG 3 cut(s) 245, 305, 685
HpyAV CCTTC 3 cut(s) 484, 631, 1595
HpyCH4III ACNGT 8 cut(s) 44, 52, 185, 364, 940, 1153, 1412, 2129
HpyCH4IV ACGT 6 cut(s) 166, 195, 309, 792, 1268, 1977
HpyF3I CTNAG 6 cut(s) 347, 488, 917, 1914, 2097, 2201
HpySE526I ACGT 6 cut(s) 166, 195, 309, 792, 1268, 1977
Hsp92I GRCGYC 5 cut(s) 195, 300, 1303, 1606, 2277
HspAI GCGC 2 cut(s) 500, 1090
Kzo9I GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
LmnI GCTCC 7 cut(s) 560, 722, 1259, 1294, 1314, 1936, 2174
Lsp1109I GCAGC 9 cut(s) 77, 94, 522, 544, 720, 742, 1264, 1804, 1873
LweI GCATC 3 cut(s) 805, 1101, 1393
MabI ACCWGGT 2 cut(s) 1744, 1957
MaeI CTAG 5 cut(s) 60, 1223, 1541, 1772, 2021
MaeII ACGT 6 cut(s) 166, 195, 309, 792, 1268, 1977
MaeIII GTNAC 5 cut(s) 65, 221, 434, 788, 973
MalI GATC 9 cut(s) 146, 383, 922, 1044, 1463, 1693, 2156, 2183, 2207
MboI GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
MboII GAAGA 5 cut(s) 612, 1202, 1541, 1667, 1990
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 3 cut(s) 920, 1042, 2205
MhlI GDGCHC 3 cut(s) 727, 1753, 1845
MluCI AATT 9 cut(s) 246, 546, 755, 999, 1360, 1500, 1755, 2110, 2323
MlyI GAGTC 4 cut(s) 1625, 1780, 1821, 2102
MmeI TCCRAC 3 cut(s) 1272, 1336, 1949
Mph1103I ATGCAT 4 cut(s) 843, 970, 1252, 1629
MslI CAYNNNNRTG 2 cut(s) 482, 1485
MspA1I CMGCKG 2 cut(s) 535, 733
MspI CCGG 6 cut(s) 75, 153, 257, 322, 1127, 1783
MspR9I CCNGG 6 cut(s) 257, 322, 1097, 1650, 1746, 1959
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 5 cut(s) 592, 778, 1063, 1459, 2315
MvaI CCWGG 4 cut(s) 1097, 1650, 1746, 1959
MvnI CGCG 1 cut(s) 1466
NciI CCSGG 2 cut(s) 257, 322
NdeII GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
NheI GCTAGC 1 cut(s) 59
NlaIV GGNNCC 3 cut(s) 1842, 1858, 2176
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 434
NsiI ATGCAT 4 cut(s) 843, 970, 1252, 1629
NspI RCATGY 1 cut(s) 908
OliI CACNNNNGTG 1 cut(s) 482
PaeI GCATGC 1 cut(s) 908
PagI TCATGA 2 cut(s) 2062, 2157
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 680
PctI GAATGC 5 cut(s) 592, 778, 1063, 1459, 2315
PfeI GAWTC 5 cut(s) 31, 853, 1340, 1439, 2197
PflFI GACNNNGTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 1095
PleI GAGTC 4 cut(s) 1625, 1780, 1821, 2101
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 4 cut(s) 1625, 1780, 1821, 2101
Ppu21I YACGTR 2 cut(s) 167, 793
PpuMI RGGWCCY 1 cut(s) 712
Psp124BI GAGCTC 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 712
Psp6I CCWGG 4 cut(s) 1095, 1648, 1744, 1957
PspCI CACGTG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 1845
PspGI CCWGG 4 cut(s) 1095, 1648, 1744, 1957
PspN4I GGNNCC 3 cut(s) 1842, 1858, 2176
PspPI GGNCC 4 cut(s) 319, 459, 712, 1990
PspPPI RGGWCCY 1 cut(s) 712
PstI CTGCAG 2 cut(s) 84, 1822
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 3 cut(s) 920, 1042, 2205
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 2 cut(s) 535, 733
RsaI GTAC 1 cut(s) 1866
RsaNI GTAC 1 cut(s) 1865
RseI CAYNNNNRTG 2 cut(s) 482, 1485
SacI GAGCTC 1 cut(s) 727
Sau3AI GATC 9 cut(s) 144, 381, 920, 1042, 1461, 1691, 2154, 2181, 2205
Sau96I GGNCC 4 cut(s) 319, 459, 712, 1990
SchI GAGTC 4 cut(s) 1625, 1780, 1821, 2102
ScrFI CCNGG 6 cut(s) 257, 322, 1097, 1650, 1746, 1959
SduI GDGCHC 3 cut(s) 727, 1753, 1845
SexAI ACCWGGT 2 cut(s) 1744, 1957
SfaNI GCATC 3 cut(s) 805, 1101, 1393
SfcI CTRYAG 3 cut(s) 80, 199, 1818
SinI GGWCC 2 cut(s) 712, 1990
SmiMI CAYNNNNRTG 2 cut(s) 482, 1485
SphI GCATGC 1 cut(s) 908
Sse9I AATT 9 cut(s) 246, 546, 755, 999, 1360, 1500, 1755, 2110, 2323
SsiI CCGC 6 cut(s) 56, 72, 417, 450, 993, 1466
SspI AATATT 2 cut(s) 1065, 1613
SspMI CTAG 5 cut(s) 60, 1223, 1541, 1772, 2021
SstI GAGCTC 1 cut(s) 727
StyD4I CCNGG 6 cut(s) 255, 320, 1095, 1648, 1744, 1957
TaaI ACNGT 8 cut(s) 44, 52, 185, 364, 940, 1153, 1412, 2129
TaiI ACGT 6 cut(s) 169, 198, 312, 795, 1271, 1980
TaqI TCGA 4 cut(s) 240, 584, 635, 1874
TasI AATT 9 cut(s) 246, 546, 755, 999, 1360, 1500, 1755, 2110, 2323
TatI WGTACW 1 cut(s) 1864
TauI GCSGC 4 cut(s) 59, 74, 419, 1469
TfiI GAWTC 5 cut(s) 31, 853, 1340, 1439, 2197
TscAI CASTG 5 cut(s) 340, 367, 487, 865, 2134
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 9 cut(s) 82, 90, 532, 535, 730, 733, 1277, 1817, 1886
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 7 cut(s) 663, 765, 828, 1020, 1214, 2174, 2279
TspGWI ACGGA 3 cut(s) 209, 1024, 1432
TspRI CASTG 5 cut(s) 340, 367, 487, 865, 2134
Tth111I GACNNNGTC 1 cut(s) 362
VneI GTGCAC 1 cut(s) 1749
VpaK11BI GGWCC 2 cut(s) 712, 1990
XapI RAATTY 2 cut(s) 1360, 2110
XbaI TCTAGA 1 cut(s) 2020
XceI RCATGY 1 cut(s) 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XmiI GTMKAC 2 cut(s) 1398, 2120
XspI CTAG 5 cut(s) 60, 1223, 1541, 1772, 2021
ZraI GACGTC 1 cut(s) 196
Zsp2I ATGCAT 4 cut(s) 843, 970, 1252, 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.