RchiOBHm_Chr7g0221631

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004093
Physical Location & Seq
Forward (+)
42689795 .. 42692438
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19832

Sequence Viewer

Length: 2286 bp
ATGATCGCTTCCGGCTTCCACCCACGTGGCCATATCCTCAACCGTTTGATCGACGTCTACTGCAAATCTTCAAATCTTCGCTACGCACTCCACCTGTTCGACCAAATTCCCAACCCGGACATCGTCGCCAGAACGACTCTGGTCTCGGCATATTCCGGCGTCGGGGACGTCACGCTGGCCCGGAGAGTCTTCAGTGAAACTCCGCTCAGCATGAGAGATACTGTTAGCTACAATGCCATGATCGCAGGCTACTCTCATAACAGGGACGGCAGTGCCGCCGTTGAGCTGTTTCGTGACATGAGGCGAGGCGGTTTTCGCCCCGATGAGTTTACCTTCACCAGTGTGCTGAGTTCTCTGGCGCTTATAGTGGAGGAGGAGAAGCAATGCCAGCAGCTGCATTGTGCAATTGTTAAGTCTGGAGCATTGCTGGCCACTTCGGTGTCGAATGCGTTGGTGTCTGTGTATGTCAAGTGTGCTTCTTCACCGTTGGTGTCGTCGTCATCTTCGGTGGTGGTGGCTGAAGCGAGGAAGGTGTTTGATGAAATGCCTAAGAGGGATGAGTTGTCGTGGACGACGATGATCACTGGTTATGTGAGGAATGAGGACCTTGATGGAGCTCGGCAGCTGCTTGATGGGATGGATGAAAAATTGGAAGTGGCATGGAATGCGATGATTTCGGGTTATGTGCGTCATGGTTTTTGTCATGAGGCATTGAAGTTGTTTAGGGAAATGCATTTAATGGGGATTCGCCAGGATGAGTTTACGTACACGAGTGTTATCAGTGCTTGTGCTAACAATGGCTTATTTAAGCATGGGAAGCAGCTGCATGCTTACATACTAAGATCTGAAGCGAAACCGACTCTGGACTTTTCTTTATCTGTGAATAATGCATTGGTGACACTATACTTTAGGTGCGGTAAAATTGATGAAGGACGATATATTTTTAATAATATGTCTGTCAGAGATCTTGTTTCTTGGAATGCGATATTGTCAGGGTATGTGACTGCAGGGCGCATTCAGGAAGCAAAGTCCTTTTTTGAGGAAATGCCGGAAAGGACTGGTCTAACGTGGACTGTAATAATATCTGGTTTAGCTCAAAATGGATGTGGGGAAGAAGCTATGAAGCTATTTAACCAAATGAGATTAGATGGTTTTAAACCCTGTGATTATGCATTTGCTGGAGCTATTACGTCTTGTGCTGCTCTTGGGGCATTGGAGCACGGACGCCAGCTCCATGCTCAACTAATATGCTTGGGGCATGATTCAAGCCTTTCAGTCGGAAATTCACTTATTACGATGTATGCGAGATGTGGGGTTGTAGATGATGCTGACTTTGTGTTCCTTACAATGCCTTATATTGATTCCGTGTCGTGGAATGCTATGATCGCAGCTCTGGCACAACATGGATATGGTGTCCAAGCAATAAGACTTTTCGAACAAATGTTGGAGGAAGATATACTGCCAGATAGAATAACTTTCCTCACTGTTCTCTCAGCTTGTAGTCATGCTGGTTTAGTTAAAGAAGGACGCCATTATTTTCGTTTAATGCATGACTCTTATGGTATTGCCCCTGGTGAAGATCATTATGCTCGTATGATTGATTTGTTGTGCCGATCTGGGGAGTTGACAGAGGCCAAGGACTTAATCAAATCAATGCCTTTTGAACCAGGTGCACCGATATGGGAGGCTATTCTAGCTGGTTGCCGGACTCATGGGAATATGGATTTAGGTATTCAAGCTGCAGAACGACTCTTTGAGCTGGTGCCCCAGCATGATGGAACCTATGTACTTTTGTCTAACTTGTATGCTGCTTTAGGCCGTTGGGATAATGTGGCTAAGGTGCGAACACTAATGAGGGAGCGAGGGGTGAAGAAAGAACCTGGTTGTAGTTGGATTGACGTTGAAAATATGGTCCATGTCTTCTTGGTTGGTGATACTGTGCACCCTGAGGTACATGAGGTGTACAAGTATCTAGAGCAACTTGTTCTTAAAATGAGGAAGTTAGGATATGTTCCTGACACAAAGTTTGCATTGCATGATATGGAGTCTGAGCATAAAGAGAATTCCTTGTCTACTCACAGTGAGAAGCTTGCAGTTGCATTTGGGATTATGAAGCTTCCTCTTGGAGCCACAATCAGAGTTTTTAAGAATCTTAGGATTTGTGGGGATTGCCATAATGCATTTAAGTTTATGTCAAGAGTGGTTGGGAGAGAAATAGTCGTGAGAGACGCCAAGAGGTTTCATCATTTTAGGAAAGGCGAATGCTCTTGTGGAAATTACTGGTAG

Protein Analysis

761

Amino Acids

85.17

Weight (kDa)

6.5

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 120 4.4e-14 PPR repeat family
PPR PF01535 75 - 105 4.4e-07 PPR repeat
PPR_2 PF13041 219 - 265 9.9e-13 PPR repeat family
PPR PF01535 219 - 249 1.1e-09 PPR repeat
PPR_2 PF13041 322 - 352 3.2e-06 PPR repeat family
PPR PF01535 324 - 352 2.9e-06 PPR repeat
PPR_2 PF13041 356 - 399 5.4e-07 PPR repeat family
PPR PF01535 356 - 385 8.3e-08 PPR repeat
PPR_2 PF13041 454 - 501 1.9e-10 PPR repeat family
PPR PF01535 456 - 486 3.2e-06 PPR repeat
E_motif PF20431 571 - 633 7e-22 E motif
DYW_deaminase PF14432 669 - 761 3.4e-42 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 57, 171
AccB1I GGYRCC 1 cut(s) 1762
AccBSI CCGCTC 1 cut(s) 205
AccI GTMKAC 2 cut(s) 57, 2072
AciI CCGC 4 cut(s) 203, 276, 309, 915
AcoI YGGCCR 2 cut(s) 28, 429
AcsI RAATTY 3 cut(s) 105, 1282, 2062
AcuI CTGAAG 3 cut(s) 175, 540, 867
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
AfaI GTAC 4 cut(s) 767, 1788, 1953, 1964
AfiI CCNNNNNNNGG 3 cut(s) 162, 1371, 1618
AgsI TTSAA 6 cut(s) 72, 715, 1266, 1664, 1736, 1904
AjnI CCWGG 4 cut(s) 750, 1570, 1666, 1879
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AleI CACNNNNGTG 3 cut(s) 24, 341, 437
Alw21I GWGCWC 4 cut(s) 619, 1221, 1675, 1944
Alw26I GTCTC 2 cut(s) 148, 2220
Alw44I GTGCAC 2 cut(s) 1671, 1940
AlwNI CAGNNNCTG 1 cut(s) 394
AoxI GGCC 5 cut(s) 28, 177, 429, 1632, 1816
ApaLI GTGCAC 2 cut(s) 1671, 1940
ApoI RAATTY 3 cut(s) 105, 1282, 2062
ArsI GACNNNNNNTTYG 2 cut(s) 2009, 2041
AspLEI GCGC 2 cut(s) 361, 1014
AspS9I GGNCC 3 cut(s) 178, 604, 1912
AsuC2I CCSGG 2 cut(s) 116, 181
AsuHPI GGTGA 6 cut(s) 328, 474, 907, 1586, 1879, 1943
AsuII TTCGAA 1 cut(s) 1434
AvaII GGWCC 2 cut(s) 604, 1912
AxyI CCTNAGG 1 cut(s) 1947
BaeGI GKGCMC 3 cut(s) 1675, 1767, 1944
BalI TGGCCA 2 cut(s) 30, 431
BanI GGYRCC 1 cut(s) 1762
BanII GRGCYC 1 cut(s) 619
BauI CACGAG 1 cut(s) 769
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 2 cut(s) 181, 1912
Bbv12I GWGCWC 4 cut(s) 619, 1221, 1675, 1944
BccI CCATC 5 cut(s) 605, 626, 631, 1142, 1769
BceAI ACGGC 3 cut(s) 263, 283, 1803
BciT130I CCWGG 4 cut(s) 752, 1572, 1668, 1881
BclI TGATCA 1 cut(s) 579
BcnI CCSGG 2 cut(s) 116, 181
BcoDI GTCTC 2 cut(s) 148, 2220
BfaI CTAG 2 cut(s) 1694, 1973
BfmI CTRYAG 2 cut(s) 1005, 1740
BfoI RGCGCY 1 cut(s) 362
BglII AGATCT 2 cut(s) 842, 964
BlpI GCTNAGC 1 cut(s) 206
Bme1390I CCNGG 6 cut(s) 116, 181, 752, 1572, 1668, 1881
Bme18I GGWCC 2 cut(s) 604, 1912
BmgT120I GGNCC 3 cut(s) 178, 604, 1912
BmiI GGNNCC 3 cut(s) 1764, 1780, 2128
BmrFI CCNGG 6 cut(s) 116, 181, 752, 1572, 1668, 1881
BmsI GCATC 1 cut(s) 1315
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 181, 1912
BpmI CTGGAG 2 cut(s) 438, 1200
Bpu10I CCTNAGC 1 cut(s) 1836
Bpu1102I GCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 1434
BpuMI CCSGG 2 cut(s) 116, 181
BsaAI YACGTR 2 cut(s) 26, 765
BsaHI GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 1570, 1635
BsaXI ACNNNNNCTCC 2 cut(s) 1215, 1245
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 1371, 1618
Bse1I ACTGG 4 cut(s) 339, 589, 1063, 2285
Bse21I CCTNAGG 1 cut(s) 1947
Bse3DI GCAATG 3 cut(s) 389, 422, 2030
BseBI CCWGG 4 cut(s) 752, 1572, 1668, 1881
BseDI CCNNGG 2 cut(s) 1570, 1635
BseGI GGATG 5 cut(s) 562, 642, 646, 760, 1109
BseLI CCNNNNNNNGG 3 cut(s) 162, 1371, 1618
BseMI GCAATG 3 cut(s) 389, 422, 2030
BseMII CTCAG 5 cut(s) 220, 338, 1506, 1938, 2040
BseNI ACTGG 4 cut(s) 339, 589, 1063, 2285
BseRI GAGGAG 2 cut(s) 386, 389
BseSI GKGCMC 3 cut(s) 1675, 1767, 1944
BseYI CCCAGC 1 cut(s) 1767
BshFI GGCC 5 cut(s) 30, 179, 431, 1634, 1818
BshNI GGYRCC 1 cut(s) 1762
BsiHKAI GWGCWC 4 cut(s) 619, 1221, 1675, 1944
BsiSI CCGG 6 cut(s) 12, 116, 156, 181, 1049, 1705
BslFI GGGAC 2 cut(s) 179, 278
BslI CCNNNNNNNGG 3 cut(s) 162, 1371, 1618
BsmAI GTCTC 2 cut(s) 148, 2220
BsmBI CGTCTC 1 cut(s) 2220
BsmFI GGGAC 2 cut(s) 179, 278
BsmI GAATGC 6 cut(s) 451, 670, 985, 1014, 1381, 2267
BsnI GGCC 5 cut(s) 30, 179, 431, 1634, 1818
Bso31I GGTCTC 1 cut(s) 148
Bsp119I TTCGAA 1 cut(s) 1434
Bsp1286I GDGCHC 5 cut(s) 619, 1221, 1675, 1767, 1944
Bsp1407I TGTACA 1 cut(s) 1962
Bsp143I GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 4 cut(s) 203, 276, 309, 915
BspANI GGCC 5 cut(s) 30, 179, 431, 1634, 1818
BspCNI CTCAG 5 cut(s) 219, 339, 1505, 1939, 2041
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 3 cut(s) 1764, 1780, 2128
BspMAI CTGCAG 2 cut(s) 1009, 1744
BspT104I TTCGAA 1 cut(s) 1434
BspT107I GGYRCC 1 cut(s) 1762
BspTNI GGTCTC 1 cut(s) 148
BsrBI CCGCTC 1 cut(s) 205
BsrDI GCAATG 3 cut(s) 389, 422, 2030
BsrGI TGTACA 1 cut(s) 1962
BsrI ACTGG 4 cut(s) 339, 589, 1063, 2285
BssECI CCNNGG 2 cut(s) 1570, 1635
BssMI GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
BssNI GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
BssSI CACGAG 1 cut(s) 769
BssT1I CCWWGG 1 cut(s) 1635
Bst2BI CACGAG 1 cut(s) 769
Bst2UI CCWGG 4 cut(s) 752, 1572, 1668, 1881
Bst4CI ACNGT 7 cut(s) 44, 223, 486, 1075, 1486, 1939, 2081
BstACI GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
BstAPI GCANNNNNTGC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 1962
BstBAI YACGTR 2 cut(s) 26, 765
BstBI TTCGAA 1 cut(s) 1434
BstC8I GCNNGC 7 cut(s) 177, 247, 389, 429, 828, 1229, 2091
BstDEI CTNAG 9 cut(s) 206, 347, 549, 839, 1492, 1836, 1947, 2049, 2153
BstF5I GGATG 5 cut(s) 562, 642, 646, 760, 1109
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 2 cut(s) 361, 1014
BstKTI GATC 9 cut(s) 6, 51, 243, 582, 845, 967, 1386, 1582, 1616
BstMAI GTCTC 2 cut(s) 148, 2220
BstMBI GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
BstNI CCWGG 4 cut(s) 752, 1572, 1668, 1881
BstNSI RCATGY 1 cut(s) 830
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 6 cut(s) 114, 179, 750, 1570, 1666, 1879
BstSFI CTRYAG 2 cut(s) 1005, 1740
BstSLI GKGCMC 3 cut(s) 1675, 1767, 1944
BstSNI TACGTA 1 cut(s) 765
BstV2I GAAGAC 2 cut(s) 181, 1912
BstX2I RGATCY 2 cut(s) 842, 964
BstXI CCANNNNNNTGG 2 cut(s) 26, 1775
BstYI RGATCY 2 cut(s) 842, 964
Bsu36I CCTNAGG 1 cut(s) 1947
BsuRI GGCC 5 cut(s) 30, 179, 431, 1634, 1818
BtgZI GCGATG 1 cut(s) 683
BtsCI GGATG 5 cut(s) 562, 642, 646, 760, 1109
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 7 cut(s) 199, 277, 346, 582, 787, 1482, 2086
Cac8I GCNNGC 7 cut(s) 177, 247, 389, 429, 828, 1229, 2091
CaiI CAGNNNCTG 1 cut(s) 394
CciI TCATGA 1 cut(s) 703
CfoI GCGC 2 cut(s) 361, 1014
Cfr13I GGNCC 3 cut(s) 178, 604, 1912
CseI GACGC 5 cut(s) 148, 677, 1233, 1536, 2237
CsiI ACCWGGT 2 cut(s) 1666, 1879
Csp6I GTAC 4 cut(s) 766, 1787, 1952, 1963
CviQI GTAC 4 cut(s) 766, 1787, 1952, 1963
DdeI CTNAG 9 cut(s) 206, 347, 549, 839, 1492, 1836, 1947, 2049, 2153
DpnI GATC 9 cut(s) 5, 50, 242, 581, 844, 966, 1385, 1581, 1615
DpnII GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
DraI TTTAAA 1 cut(s) 1156
EaeI YGGCCR 2 cut(s) 28, 429
Ecl136II GAGCTC 1 cut(s) 617
Eco105I TACGTA 1 cut(s) 765
Eco130I CCWWGG 1 cut(s) 1635
Eco24I GRGCYC 1 cut(s) 619
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 604, 1912
Eco53kI GAGCTC 1 cut(s) 617
Eco57I CTGAAG 3 cut(s) 175, 540, 867
Eco72I CACGTG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 1947
EcoICRI GAGCTC 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 2062
EcoRII CCWGG 4 cut(s) 750, 1570, 1666, 1879
EcoT14I CCWWGG 1 cut(s) 1635
EcoT22I ATGCAT 5 cut(s) 735, 892, 1174, 1551, 2182
EcoT38I GRGCYC 1 cut(s) 619
ErhI CCWWGG 1 cut(s) 1635
Esp3I CGTCTC 1 cut(s) 2220
FaqI GGGAC 2 cut(s) 179, 278
FbaI TGATCA 1 cut(s) 579
FblI GTMKAC 2 cut(s) 57, 2072
FokI GGATG 5 cut(s) 569, 649, 653, 767, 1116
FriOI GRGCYC 1 cut(s) 619
FspBI CTAG 2 cut(s) 1694, 1973
GlaI GCGC 2 cut(s) 360, 1013
GsaI CCCAGC 1 cut(s) 1771
GsuI CTGGAG 2 cut(s) 438, 1200
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 5 cut(s) 30, 179, 431, 1634, 1818
HapII CCGG 6 cut(s) 12, 116, 156, 181, 1049, 1705
HgaI GACGC 5 cut(s) 148, 677, 1233, 1536, 2237
HhaI GCGC 2 cut(s) 361, 1014
Hin1I GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
Hin6I GCGC 2 cut(s) 359, 1012
HinP1I GCGC 2 cut(s) 359, 1012
HincII GTYRAC 1 cut(s) 1626
HindII GTYRAC 1 cut(s) 1626
HindIII AAGCTT 2 cut(s) 2087, 2114
HpaII CCGG 6 cut(s) 12, 116, 156, 181, 1049, 1705
HphI GGTGA 6 cut(s) 328, 474, 907, 1586, 1879, 1943
Hpy188I TCNGA 5 cut(s) 847, 962, 1280, 2050, 2138
Hpy188III TCNNGA 9 cut(s) 293, 417, 704, 863, 1019, 1973, 2015, 2196, 2221
Hpy99I CGWCG 5 cut(s) 56, 128, 164, 499, 577
HpyAV CCTTC 4 cut(s) 343, 523, 923, 1517
HpyCH4III ACNGT 7 cut(s) 44, 223, 486, 1075, 1486, 1939, 2081
HpyCH4IV ACGT 7 cut(s) 25, 54, 168, 764, 1067, 1190, 1899
HpyF3I CTNAG 9 cut(s) 206, 347, 549, 839, 1492, 1836, 1947, 2049, 2153
HpySE526I ACGT 7 cut(s) 25, 54, 168, 764, 1067, 1190, 1899
Hsp92I GRCGYC 6 cut(s) 54, 159, 168, 1225, 1528, 2229
HspAI GCGC 2 cut(s) 359, 1012
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
LmnI GCTCC 7 cut(s) 419, 614, 1181, 1216, 1236, 1858, 2126
LweI GCATC 1 cut(s) 1315
MabI ACCWGGT 2 cut(s) 1666, 1879
MaeI CTAG 2 cut(s) 1694, 1973
MaeII ACGT 7 cut(s) 25, 54, 168, 764, 1067, 1190, 1899
MaeIII GTNAC 4 cut(s) 169, 293, 895, 1000
MalI GATC 9 cut(s) 5, 50, 242, 581, 844, 966, 1385, 1581, 1615
MbiI CCGCTC 1 cut(s) 205
MboI GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 842, 964
MhlI GDGCHC 5 cut(s) 619, 1221, 1675, 1767, 1944
MlsI TGGCCA 2 cut(s) 30, 431
MluCI AATT 7 cut(s) 105, 405, 647, 921, 1282, 2062, 2275
MluNI TGGCCA 2 cut(s) 30, 431
MlyI GAGTC 7 cut(s) 130, 195, 853, 1547, 1702, 1743, 2054
MmeI TCCRAC 3 cut(s) 1258, 1425, 1871
Mox20I TGGCCA 2 cut(s) 30, 431
Mph1103I ATGCAT 5 cut(s) 735, 892, 1174, 1551, 2182
MscI TGGCCA 2 cut(s) 30, 431
MslI CAYNNNNRTG 5 cut(s) 24, 341, 437, 1407, 1678
Msp20I TGGCCA 2 cut(s) 30, 431
MspA1I CMGCKG 3 cut(s) 394, 625, 823
MspI CCGG 6 cut(s) 12, 116, 156, 181, 1049, 1705
MspR9I CCNGG 6 cut(s) 116, 181, 752, 1572, 1668, 1881
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 6 cut(s) 451, 670, 985, 1014, 1381, 2267
MvaI CCWGG 4 cut(s) 752, 1572, 1668, 1881
NciI CCSGG 2 cut(s) 116, 181
NdeII GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
NlaIV GGNNCC 3 cut(s) 1764, 1780, 2128
NmeAIII GCCGAG 2 cut(s) 125, 598
NmuCI GTSAC 4 cut(s) 169, 293, 895, 1000
NsiI ATGCAT 5 cut(s) 735, 892, 1174, 1551, 2182
NspI RCATGY 1 cut(s) 830
NspV TTCGAA 1 cut(s) 1434
OliI CACNNNNGTG 3 cut(s) 24, 341, 437
PaeI GCATGC 1 cut(s) 830
PagI TCATGA 1 cut(s) 703
PcsI WCGNNNNNNNCGW 2 cut(s) 572, 1301
PctI GAATGC 6 cut(s) 451, 670, 985, 1014, 1381, 2267
PfeI GAWTC 4 cut(s) 745, 1262, 1361, 2149
PflFI GACNNNGTC 1 cut(s) 122
PleI GAGTC 7 cut(s) 130, 194, 853, 1547, 1702, 1743, 2053
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 7 cut(s) 130, 194, 853, 1547, 1702, 1743, 2053
Ppu21I YACGTR 2 cut(s) 26, 765
PpuMI RGGWCCY 1 cut(s) 604
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 4 cut(s) 750, 1570, 1666, 1879
PspCI CACGTG 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 1767
PspGI CCWGG 4 cut(s) 750, 1570, 1666, 1879
PspN4I GGNNCC 3 cut(s) 1764, 1780, 2128
PspPI GGNCC 3 cut(s) 178, 604, 1912
PspPPI RGGWCCY 1 cut(s) 604
PstI CTGCAG 2 cut(s) 1009, 1744
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 842, 964
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 3 cut(s) 394, 625, 823
RsaI GTAC 4 cut(s) 767, 1788, 1953, 1964
RsaNI GTAC 4 cut(s) 766, 1787, 1952, 1963
RseI CAYNNNNRTG 5 cut(s) 24, 341, 437, 1407, 1678
SacI GAGCTC 1 cut(s) 619
Sau3AI GATC 9 cut(s) 3, 48, 240, 579, 842, 964, 1383, 1579, 1613
Sau96I GGNCC 3 cut(s) 178, 604, 1912
SchI GAGTC 7 cut(s) 130, 195, 853, 1547, 1702, 1743, 2054
ScrFI CCNGG 6 cut(s) 116, 181, 752, 1572, 1668, 1881
SduI GDGCHC 5 cut(s) 619, 1221, 1675, 1767, 1944
SexAI ACCWGGT 2 cut(s) 1666, 1879
SfaNI GCATC 1 cut(s) 1315
SfcI CTRYAG 2 cut(s) 1005, 1740
SfuI TTCGAA 1 cut(s) 1434
SinI GGWCC 2 cut(s) 604, 1912
SmiMI CAYNNNNRTG 5 cut(s) 24, 341, 437, 1407, 1678
SnaBI TACGTA 1 cut(s) 765
SphI GCATGC 1 cut(s) 830
Sse9I AATT 7 cut(s) 105, 405, 647, 921, 1282, 2062, 2275
SsiI CCGC 4 cut(s) 203, 276, 309, 915
SspMI CTAG 2 cut(s) 1694, 1973
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 6 cut(s) 114, 179, 750, 1570, 1666, 1879
StyI CCWWGG 1 cut(s) 1635
TaaI ACNGT 7 cut(s) 44, 223, 486, 1075, 1486, 1939, 2081
TaiI ACGT 7 cut(s) 28, 57, 171, 767, 1070, 1193, 1902
TaqI TCGA 4 cut(s) 51, 99, 443, 1434
TasI AATT 7 cut(s) 105, 405, 647, 921, 1282, 2062, 2275
TatI WGTACW 2 cut(s) 1786, 1962
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 4 cut(s) 745, 1262, 1361, 2149
TscAI CASTG 7 cut(s) 199, 277, 346, 589, 787, 1489, 2086
TseFI GTSAC 4 cut(s) 169, 293, 895, 1000
Tsp45I GTSAC 4 cut(s) 169, 293, 895, 1000
TspDTI ATGAA 6 cut(s) 555, 657, 942, 1136, 2126, 2231
TspGWI ACGGA 2 cut(s) 1236, 1354
TspRI CASTG 7 cut(s) 199, 277, 346, 589, 787, 1489, 2086
Tth111I GACNNNGTC 1 cut(s) 122
VneI GTGCAC 2 cut(s) 1671, 1940
VpaK11BI GGWCC 2 cut(s) 604, 1912
XapI RAATTY 3 cut(s) 105, 1282, 2062
XbaI TCTAGA 1 cut(s) 1972
XceI RCATGY 1 cut(s) 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmiI GTMKAC 2 cut(s) 57, 2072
XspI CTAG 2 cut(s) 1694, 1973
ZraI GACGTC 2 cut(s) 55, 169
Zsp2I ATGCAT 5 cut(s) 735, 892, 1174, 1551, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.