pycom17g27140

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003430
Physical Location & Seq
Reverse (-)
25390503 .. 25392507
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGGGAGTGCTGTCGAACAAGATCCAGAGGGATCTTCTGAAGCCTGGAGATCACATCTATTCCTGGCGCCACGCCTACCTTTATGCCCACCACGGGATATATGTTGGTGAAGGAAAGGTTATTCACTTCACTCGAGGAGCGGGCCAGGAAATTGGCACAGGAACTGTGTTAGACCGCTTCCTCATAAGCAGCTCTCCATCCCATTCTCCGGACAATCCCTGCCCAAACTGCGGCGATCTATCAACAGTTGATGGTGTCATGTCTTCTTGCATCGATTGTTTTCTATCTGGCGGTTATCTTTACCTCTTTGAATATGGTGTCACTCCAGCTTTCTTTGTTGCCAAAGCTAGAGGGGGTACCTGTACCCTTGCGCGGTCTGACCCTCCCGAAGATGTCCTTCACCGTGCTTCTTTCCTTCTGCAGAATGGCTTTGGTAGTTATGACATTTTCAAAAACAACTGTGAAGATTTCGCCATATATTGCAAAACAGGCTTGCTCGTGTTCACCAGCATTAGCGTGGGCAGGAGTGGACAGGCTGCCTCCTTCGTAGCTGCCGCAACTGCTGCTGTTTCTTCACCGCTTCGATTTTTGACTACCAGTTTTACTGGTCTGGGTGCTGTAGGTTGTGGCATGTATTGTGTTAGTAGATTGGTTTCTGACATCGGAATACGCCGAGATGTTAGGAAAGTATCGGTGGAGAGACTGATTACCCCCTGTGCCCTTGACGAGTCAGTGGTTGCAACTGAATTGCTCAAGAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

255

Amino Acids

27.36

Weight (kDa)

7.02

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 13 - 166 1.9e-29 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 2 cut(s) 66, 356
AccBSI CCGCTC 1 cut(s) 140
AccII CGCG 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 7 cut(s) 140, 175, 231, 291, 373, 555, 578
AclWI GGATC 2 cut(s) 16, 39
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 358, 364
AfiI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
AgsI TTSAA 2 cut(s) 311, 451
AjnI CCWGG 3 cut(s) 43, 62, 144
AluBI AGCT 4 cut(s) 192, 329, 347, 551
AluI AGCT 4 cut(s) 192, 329, 347, 551
Alw26I GTCTC 1 cut(s) 694
AlwI GGATC 2 cut(s) 16, 39
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 132
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 4 cut(s) 189, 536, 551, 563
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 69, 373
AspS9I GGNCC 1 cut(s) 142
AsuHPI GGTGA 4 cut(s) 119, 392, 496, 567
AvaI CYCGRG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 721
BanI GGYRCC 2 cut(s) 66, 356
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 201, 523, 538, 550
BccI CCATC 2 cut(s) 205, 245
BciT130I CCWGG 3 cut(s) 45, 64, 146
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 2 cut(s) 419, 618
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 6 cut(s) 190, 232, 537, 552, 555, 564
BlsI GCNGC 6 cut(s) 191, 233, 538, 553, 556, 565
Bme1390I CCNGG 3 cut(s) 45, 64, 146
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 68, 358
BmrFI CCNGG 3 cut(s) 45, 64, 146
BmsI GCATC 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 2 cut(s) 66, 309
BpuEI CTTGAG 1 cut(s) 737
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
Bse1I ACTGG 2 cut(s) 597, 610
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 3 cut(s) 45, 64, 146
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
BseNI ACTGG 2 cut(s) 597, 610
BseRI GAGGAG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 4 cut(s) 201, 523, 538, 550
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 2 cut(s) 66, 356
BshVI ATCGAT 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
BsmAI GTCTC 1 cut(s) 694
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 721
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 4 cut(s) 21, 31, 49, 235
BspACI CCGC 7 cut(s) 140, 175, 231, 291, 373, 555, 578
BspANI GGCC 1 cut(s) 144
BspDI ATCGAT 1 cut(s) 273
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 68, 358
BspMAI CTGCAG 1 cut(s) 423
BspPI GGATC 2 cut(s) 16, 39
BspT107I GGYRCC 2 cut(s) 66, 356
BsrBI CCGCTC 1 cut(s) 140
BsrI ACTGG 2 cut(s) 597, 610
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 4 cut(s) 21, 31, 49, 235
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 3 cut(s) 45, 64, 146
Bst4CI ACNGT 4 cut(s) 166, 247, 404, 461
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 142, 494
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 2 cut(s) 69, 373
BstKTI GATC 4 cut(s) 24, 34, 52, 238
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 4 cut(s) 21, 31, 49, 235
BstMWI GCNNNNNNNGC 4 cut(s) 228, 489, 560, 563
BstNI CCWGG 3 cut(s) 45, 64, 146
BstNSI RCATGY 1 cut(s) 634
BstSCI CCNGG 3 cut(s) 43, 62, 144
BstSFI CTRYAG 2 cut(s) 419, 618
BstSLI GKGCMC 1 cut(s) 721
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 4 cut(s) 201, 523, 538, 550
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 2 cut(s) 21, 31
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 2 cut(s) 21, 31
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 738
Cac8I GCNNGC 2 cut(s) 142, 494
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 2 cut(s) 69, 373
Cfr13I GGNCC 1 cut(s) 142
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 357, 363
CviAII CATG 2 cut(s) 259, 631
CviJI RGCY 9 cut(s) 43, 144, 192, 329, 347, 429, 492, 536, 551
CviKI_1 RGCY 9 cut(s) 43, 144, 192, 329, 347, 429, 492, 536, 551
CviQI GTAC 2 cut(s) 357, 363
DinI GGCGCC 1 cut(s) 68
DpnI GATC 4 cut(s) 23, 33, 51, 237
DpnII GATC 4 cut(s) 21, 31, 49, 235
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 3 cut(s) 43, 62, 144
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
FaeI CATG 2 cut(s) 262, 634
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 258, 630
FauI CCCGC 1 cut(s) 133
Fnu4HI GCNGC 6 cut(s) 190, 232, 537, 552, 555, 564
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 6 cut(s) 190, 232, 537, 552, 555, 564
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 68, 372
GluI GCNGC 6 cut(s) 190, 232, 537, 552, 555, 564
GsuI CTGGAG 2 cut(s) 66, 309
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 1 cut(s) 144
HapII CCGG 1 cut(s) 209
HhaI GCGC 2 cut(s) 69, 373
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 2 cut(s) 262, 634
Hin6I GCGC 2 cut(s) 67, 371
HinP1I GCGC 2 cut(s) 67, 371
HinfI GANTC 1 cut(s) 728
HpaII CCGG 1 cut(s) 209
HphI GGTGA 4 cut(s) 119, 392, 496, 567
Hpy166II GTNNAC 2 cut(s) 504, 530
Hpy188I TCNGA 4 cut(s) 39, 379, 658, 665
Hpy188III TCNNGA 4 cut(s) 25, 209, 386, 754
Hpy8I GTNNAC 2 cut(s) 504, 530
HpyAV CCTTC 4 cut(s) 104, 407, 425, 553
HpyCH4III ACNGT 4 cut(s) 166, 247, 404, 461
HpyCH4V TGCA 4 cut(s) 270, 421, 483, 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 228, 489, 560, 563
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 2 cut(s) 262, 634
HspAI GCGC 2 cut(s) 67, 371
KasI GGCGCC 1 cut(s) 66
Kpn2I TCCGGA 1 cut(s) 208
KpnI GGTACC 1 cut(s) 360
Kzo9I GATC 4 cut(s) 21, 31, 49, 235
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 4 cut(s) 201, 523, 538, 550
LweI GCATC 1 cut(s) 279
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 23, 33, 51, 237
MbiI CCGCTC 1 cut(s) 140
MboI GATC 4 cut(s) 21, 31, 49, 235
MboII GAAGA 5 cut(s) 26, 255, 401, 476, 564
MflI RGATCY 2 cut(s) 21, 31
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 2 cut(s) 150, 746
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 7 cut(s) 21, 128, 191, 314, 344, 393, 550
MroI TCCGGA 1 cut(s) 208
MslI CAYNNNNRTG 1 cut(s) 515
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 3 cut(s) 45, 64, 146
MvaI CCWGG 3 cut(s) 45, 64, 146
MvnI CGCG 1 cut(s) 373
MwoI GCNNNNNNNGC 4 cut(s) 228, 489, 560, 563
NarI GGCGCC 1 cut(s) 67
NdeII GATC 4 cut(s) 21, 31, 49, 235
NlaIII CATG 2 cut(s) 262, 634
NlaIV GGNNCC 2 cut(s) 68, 358
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 634
PaeR7I CTCGAG 1 cut(s) 132
PkrI GCNGC 6 cut(s) 191, 233, 538, 553, 556, 565
PleI GAGTC 1 cut(s) 736
PluTI GGCGCC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 736
Psp6I CCWGG 3 cut(s) 43, 62, 144
PspGI CCWGG 3 cut(s) 43, 62, 144
PspN4I GGNNCC 2 cut(s) 68, 358
PspPI GGNCC 1 cut(s) 142
PspXI VCTCGAGB 1 cut(s) 132
PstI CTGCAG 1 cut(s) 423
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 21, 31
RsaI GTAC 2 cut(s) 358, 364
RsaNI GTAC 2 cut(s) 357, 363
RseI CAYNNNNRTG 1 cut(s) 515
SatI GCNGC 6 cut(s) 190, 232, 537, 552, 555, 564
Sau3AI GATC 4 cut(s) 21, 31, 49, 235
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 3 cut(s) 45, 64, 146
SduI GDGCHC 1 cut(s) 721
SetI ASST 9 cut(s) 81, 120, 194, 306, 331, 349, 362, 553, 625
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 2 cut(s) 419, 618
SfoI GGCGCC 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 2 cut(s) 132, 752
SmoI CTYRAG 2 cut(s) 132, 752
Sse9I AATT 2 cut(s) 150, 746
SsiI CCGC 7 cut(s) 140, 175, 231, 291, 373, 555, 578
SspDI GGCGCC 1 cut(s) 66
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 43, 62, 144
TaaI ACNGT 4 cut(s) 166, 247, 404, 461
TaqI TCGA 4 cut(s) 14, 133, 273, 583
TasI AATT 2 cut(s) 150, 746
TauI GCSGC 2 cut(s) 234, 557
TscAI CASTG 1 cut(s) 738
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 4 cut(s) 189, 536, 551, 563
Tsp45I GTSAC 1 cut(s) 319
TspRI CASTG 1 cut(s) 738
XceI RCATGY 1 cut(s) 634
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XhoI CTCGAG 1 cut(s) 132
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.