RchiOBHm_Chr5g0066831

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004885
Physical Location & Seq
Reverse (-)
72913167 .. 72914138
972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGGATTCCCGGCACGCATCTCTAGGCCGCCGAACGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGGCCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGTAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGCTTGAAACAAAGGAAGCCGTTGCCCCGGTTTACTTTGTTTGCTGTAATTTAGGAGCAAAATACTGTACCGTCTTTGAGGAAAGCTCTCAAGGATGTCGCAATGGAGAAAGATGCTGCAGTTGTTGCACGGGAGGATCTCTCTGCCCAGCTGCGTATGCTCAAGAAACGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.66

Weight (kDa)

5.92

Isoelectric Point (pI)

59.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 28
AclI AACGTT 1 cut(s) 35
AclWI GGATC 2 cut(s) 103, 400
AcsI RAATTY 2 cut(s) 45, 244
AfaI GTAC 2 cut(s) 181, 325
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 1 cut(s) 263
AluBI AGCT 3 cut(s) 62, 342, 407
AluI AGCT 3 cut(s) 62, 342, 407
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 2 cut(s) 103, 400
AoxI GGCC 2 cut(s) 25, 92
ApaI GGGCCC 1 cut(s) 96
ApeKI GCWGC 3 cut(s) 62, 372, 407
ApoI RAATTY 2 cut(s) 45, 244
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 92, 93
AsuC2I CCSGG 2 cut(s) 10, 284
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 96
BbvI GCAGC 3 cut(s) 49, 359, 394
BceAI ACGGC 1 cut(s) 260
BcnI CCSGG 2 cut(s) 10, 284
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 2 cut(s) 63, 373
BisI GCNGC 4 cut(s) 28, 63, 373, 408
BlsI GCNGC 4 cut(s) 29, 64, 374, 409
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 10, 284
BmgT120I GGNCC 2 cut(s) 92, 93
BmiI GGNNCC 2 cut(s) 93, 94
BmrFI CCNGG 2 cut(s) 10, 284
BmsI GCATC 2 cut(s) 26, 359
BplI GAGNNNNNCTC 4 cut(s) 326, 358, 381, 413
BpuEI CTTGAG 2 cut(s) 330, 402
BpuMI CCSGG 2 cut(s) 10, 284
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 364
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 356
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 3 cut(s) 115, 156, 206
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 3 cut(s) 49, 359, 394
BseYI CCCAGC 1 cut(s) 403
BshFI GGCC 2 cut(s) 27, 94
BshVI ATCGAT 1 cut(s) 120
BsiSI CCGG 2 cut(s) 10, 284
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 195
BsnI GGCC 2 cut(s) 27, 94
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 97, 108, 392
BspACI CCGC 1 cut(s) 28
BspANI GGCC 2 cut(s) 27, 94
BspCNI CTCAG 3 cut(s) 114, 157, 205
BspDI ATCGAT 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 93, 94
BspMAI CTGCAG 2 cut(s) 67, 377
BspPI GGATC 2 cut(s) 103, 400
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 3 cut(s) 97, 108, 392
Bst4CI ACNGT 2 cut(s) 323, 328
BstAPI GCANNNNNTGC 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 15, 60
BstDEI CTNAG 3 cut(s) 101, 165, 192
BstF5I GGATG 1 cut(s) 356
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 100, 111, 395
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 3 cut(s) 97, 108, 392
BstMWI GCNNNNNNNGC 2 cut(s) 381, 413
BstSCI CCNGG 2 cut(s) 8, 282
BstSFI CTRYAG 2 cut(s) 63, 373
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 49, 359, 394
BstX2I RGATCY 2 cut(s) 108, 392
BstYI RGATCY 2 cut(s) 108, 392
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 2 cut(s) 27, 94
BsuTUI ATCGAT 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 15, 60
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 92, 93
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 2 cut(s) 180, 324
CviAII CATG 1 cut(s) 256
CviJI RGCY 8 cut(s) 27, 62, 94, 105, 251, 275, 342, 407
CviKI_1 RGCY 8 cut(s) 27, 62, 94, 105, 251, 275, 342, 407
CviQI GTAC 2 cut(s) 180, 324
DdeI CTNAG 3 cut(s) 101, 165, 192
DpnI GATC 3 cut(s) 99, 110, 394
DpnII GATC 3 cut(s) 97, 108, 392
Eco24I GRGCYC 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 1 cut(s) 259
FaiI YATR 2 cut(s) 257, 414
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 4 cut(s) 28, 63, 373, 408
FokI GGATG 1 cut(s) 363
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 4 cut(s) 28, 63, 373, 408
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 151
GluI GCNGC 4 cut(s) 28, 63, 373, 408
GsaI CCCAGC 1 cut(s) 407
HaeIII GGCC 2 cut(s) 27, 94
HapII CCGG 2 cut(s) 10, 284
HhaI GCGC 1 cut(s) 152
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 5, 159
HpaII CCGG 2 cut(s) 10, 284
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 145, 166, 195
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 323, 328
HpyCH4IV ACGT 2 cut(s) 35, 425
HpyCH4V TGCA 3 cut(s) 65, 375, 384
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 413
HpyF3I CTNAG 3 cut(s) 101, 165, 192
HpySE526I ACGT 2 cut(s) 35, 425
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 97, 108, 392
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 5 cut(s) 23, 119, 212, 297, 417
Lsp1109I GCAGC 3 cut(s) 49, 359, 394
LweI GCATC 2 cut(s) 26, 359
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 35, 425
MalI GATC 3 cut(s) 99, 110, 394
MboI GATC 3 cut(s) 97, 108, 392
MboII GAAGA 4 cut(s) 53, 78, 226, 242
MflI RGATCY 2 cut(s) 108, 392
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 45, 244, 304
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 3 cut(s) 122, 328, 383
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 2 cut(s) 10, 284
MspR9I CCNGG 2 cut(s) 10, 284
MwoI GCNNNNNNNGC 2 cut(s) 381, 413
NciI CCSGG 2 cut(s) 10, 284
NdeII GATC 3 cut(s) 97, 108, 392
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 93, 94
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 4 cut(s) 29, 64, 374, 409
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 35
PspFI CCCAGC 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 93, 94
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 92, 93
PstI CTGCAG 2 cut(s) 67, 377
PsuI RGATCY 2 cut(s) 108, 392
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 181, 325
RsaNI GTAC 2 cut(s) 180, 324
SaqAI TTAA 1 cut(s) 430
SatI GCNGC 4 cut(s) 28, 63, 373, 408
Sau3AI GATC 3 cut(s) 97, 108, 392
Sau96I GGNCC 2 cut(s) 92, 93
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 2 cut(s) 10, 284
SduI GDGCHC 1 cut(s) 96
SetI ASST 6 cut(s) 38, 64, 118, 344, 409, 428
SfaNI GCATC 2 cut(s) 26, 359
SfcI CTRYAG 2 cut(s) 63, 373
SmlI CTYRAG 2 cut(s) 345, 417
SmoI CTYRAG 2 cut(s) 345, 417
Sse9I AATT 3 cut(s) 45, 244, 304
SsiI CCGC 1 cut(s) 28
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 8, 282
TaaI ACNGT 2 cut(s) 323, 328
TaiI ACGT 2 cut(s) 38, 428
TaqI TCGA 2 cut(s) 120, 157
TasI AATT 3 cut(s) 45, 244, 304
TatI WGTACW 1 cut(s) 179
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 430
Tru9I TTAA 1 cut(s) 430
TseI GCWGC 3 cut(s) 62, 372, 407
XapI RAATTY 2 cut(s) 45, 244
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.