Rroxscaffold_2G00137040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004885
Physical Location & Seq
Forward (+)
74755842 .. 74756647
806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137040.1

Sequence Viewer

Length: 498 bp
ATGGATTCACGGCACGCATCTCTAGGCCGCCGAACGTTGGAAGAAATTTGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGAACCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGGTAGGACTCTTGTGTTTGTTGTGCTTGTTTGTTTCTTTTCCGAAAGTTTTCACTTCAGGAGGGTTTGGTTGTGGGCAATGGTCAGTTTGGTTGCCTAATTATATTGTCGACTTATACAGGCTGGGCAAAGAGGCCGATAACAACAACATCGCCGTAGGATCCAGTGGCGGCGTTGTCGATAATCTTTTTGTCGGCAAATCCCTCAATCGAGACTCCGATATCTTCTTTGATTATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.3

Weight (kDa)

5.49

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 366
AciI CCGC 2 cut(s) 28, 426
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 103, 411, 424
AcsI RAATTY 2 cut(s) 45, 244
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 1 cut(s) 37
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 195, 462
AlwI GGATC 3 cut(s) 103, 411, 424
AoxI GGCC 2 cut(s) 25, 390
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 2 cut(s) 45, 244
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 1 cut(s) 152
BamHI GGATCC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 49
BceAI ACGGC 2 cut(s) 26, 395
BcoDI GTCTC 2 cut(s) 195, 462
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 3 cut(s) 28, 63, 427
BlsI GCNGC 3 cut(s) 29, 64, 428
BmcAI AGTACT 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 93, 418
BmsI GCATC 1 cut(s) 26
Bsa29I ATCGAT 2 cut(s) 120, 493
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 231, 420
Bse3DI GCAATG 1 cut(s) 341
BseCI ATCGAT 2 cut(s) 120, 493
BseDI CCNNGG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 3 cut(s) 115, 156, 206
BseNI ACTGG 2 cut(s) 231, 420
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 379
BshFI GGCC 2 cut(s) 27, 392
BshVI ATCGAT 2 cut(s) 120, 493
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 195, 462
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 27, 392
Bsp143I GATC 3 cut(s) 97, 108, 416
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 2 cut(s) 28, 426
BspANI GGCC 2 cut(s) 27, 392
BspCNI CTCAG 3 cut(s) 114, 157, 205
BspDI ATCGAT 2 cut(s) 120, 493
BspLI GGNNCC 2 cut(s) 93, 418
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 3 cut(s) 103, 411, 424
BsrDI GCAATG 1 cut(s) 341
BsrI ACTGG 2 cut(s) 231, 420
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 3 cut(s) 97, 108, 416
BssT1I CCWWGG 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 15, 60
BstDEI CTNAG 3 cut(s) 101, 165, 192
BstDSI CCRYGG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 100, 111, 419
BstMAI GTCTC 2 cut(s) 195, 462
BstMBI GATC 3 cut(s) 97, 108, 416
BstSFI CTRYAG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 49
BstX2I RGATCY 2 cut(s) 108, 416
BstYI RGATCY 2 cut(s) 108, 416
Bsu15I ATCGAT 2 cut(s) 120, 493
BsuRI GGCC 2 cut(s) 27, 392
BsuTUI ATCGAT 2 cut(s) 120, 493
BtgI CCRYGG 1 cut(s) 255
BtgZI GCGATG 2 cut(s) 186, 391
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 15, 60
CfoI GCGC 1 cut(s) 152
ClaI ATCGAT 2 cut(s) 120, 493
Csp6I GTAC 1 cut(s) 180
CviAII CATG 1 cut(s) 256
CviJI RGCY 6 cut(s) 27, 62, 105, 251, 379, 392
CviKI_1 RGCY 6 cut(s) 27, 62, 105, 251, 379, 392
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 3 cut(s) 101, 165, 192
DpnI GATC 3 cut(s) 99, 110, 418
DpnII GATC 3 cut(s) 97, 108, 416
Eco130I CCWWGG 1 cut(s) 255
Eco32I GATATC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 297
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 1 cut(s) 259
FaiI YATR 3 cut(s) 257, 360, 373
FatI CATG 1 cut(s) 255
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 28, 63, 427
Fsp4HI GCNGC 3 cut(s) 28, 63, 427
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 28, 63, 427
GsaI CCCAGC 1 cut(s) 383
HaeIII GGCC 2 cut(s) 27, 392
HhaI GCGC 1 cut(s) 152
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 4 cut(s) 5, 159, 264, 470
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 6 cut(s) 91, 145, 166, 195, 300, 475
Hpy188III TCNNGA 2 cut(s) 315, 467
Hpy8I GTNNAC 1 cut(s) 367
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 65, 221
HpyF3I CTNAG 3 cut(s) 101, 165, 192
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 97, 108, 416
LpnPI CCDG 6 cut(s) 119, 212, 300, 361, 365, 433
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 35
MalI GATC 3 cut(s) 99, 110, 418
MboI GATC 3 cut(s) 97, 108, 416
MboII GAAGA 5 cut(s) 53, 78, 226, 242, 472
MflI RGATCY 2 cut(s) 108, 416
MluCI AATT 3 cut(s) 45, 244, 355
MlyI GAGTC 3 cut(s) 153, 258, 464
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 4 cut(s) 122, 311, 382, 470
MroXI GAANNNNTTC 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 223
NcoI CCATGG 1 cut(s) 255
NdeII GATC 3 cut(s) 97, 108, 416
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 93, 418
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 3 cut(s) 29, 64, 428
PleI GAGTC 3 cut(s) 153, 258, 464
PpsI GAGTC 3 cut(s) 153, 258, 464
Psp1406I AACGTT 1 cut(s) 35
PspFI CCCAGC 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 93, 418
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 2 cut(s) 108, 416
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
SalI GTCGAC 1 cut(s) 365
SatI GCNGC 3 cut(s) 28, 63, 427
Sau3AI GATC 3 cut(s) 97, 108, 416
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 3 cut(s) 153, 258, 464
SetI ASST 3 cut(s) 38, 64, 118
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 1 cut(s) 63
Sse9I AATT 3 cut(s) 45, 244, 355
SsiI CCGC 2 cut(s) 28, 426
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 1 cut(s) 38
TaqI TCGA 6 cut(s) 120, 157, 366, 435, 466, 493
TasI AATT 3 cut(s) 45, 244, 355
TatI WGTACW 1 cut(s) 179
TauI GCSGC 2 cut(s) 30, 429
TfiI GAWTC 1 cut(s) 5
TscAI CASTG 1 cut(s) 427
TseI GCWGC 1 cut(s) 62
TspRI CASTG 1 cut(s) 427
XapI RAATTY 2 cut(s) 45, 244
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.