Rw0G020530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00933
OG0004885
Physical Location & Seq
Reverse (-)
12711 .. 13620
910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020530.1

Sequence Viewer

Length: 423 bp
ATGCTTCGAGGCGTGGCTGGAGGAGGTGCGACCTGGCTTGGACGCGTGGGGAAAGGCAGTGCAGAGGCTTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGACCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTAAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGGTAGGACCCTTGCTGGGCAAAGAGGCCGATAACAACAACATCGCCGTAGGATCCCGGGGCGGCGGCGTCGATAATCTTTTTGTCGGCAAATCCCTCAATCGAGACTCCGATATCGTCTTTGATTATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

14.95

Weight (kDa)

9.04

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 45
AciI CCGC 2 cut(s) 351, 354
AclWI GGATC 3 cut(s) 136, 336, 349
AcsI RAATTY 2 cut(s) 78, 277
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 43
AjnI CCWGG 1 cut(s) 32
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 228, 387
AlwI GGATC 3 cut(s) 136, 336, 349
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 78, 277
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 125, 296
AsuC2I CCSGG 2 cut(s) 346, 347
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 2 cut(s) 125, 296
BamHI GGATCC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 34
BcnI CCSGG 2 cut(s) 346, 347
BcoDI GTCTC 2 cut(s) 228, 387
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 3 cut(s) 96, 352, 355
BlsI GCNGC 3 cut(s) 97, 353, 356
BmcAI AGTACT 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 34, 346, 347
Bme18I GGWCC 2 cut(s) 125, 296
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 2 cut(s) 125, 296
BmiI GGNNCC 4 cut(s) 126, 127, 298, 343
BmrFI CCNGG 3 cut(s) 34, 346, 347
BpmI CTGGAG 1 cut(s) 39
BpuMI CCSGG 2 cut(s) 346, 347
Bsa29I ATCGAT 2 cut(s) 153, 418
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 288, 345, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 264
BseBI CCWGG 1 cut(s) 34
BseCI ATCGAT 2 cut(s) 153, 418
BseDI CCNNGG 3 cut(s) 288, 345, 346
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 2 cut(s) 148, 239
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 317
BshVI ATCGAT 2 cut(s) 153, 418
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 1 cut(s) 346
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 228, 387
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 3 cut(s) 130, 141, 341
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 2 cut(s) 351, 354
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 147, 238
BspDI ATCGAT 2 cut(s) 153, 418
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 4 cut(s) 126, 127, 298, 343
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 3 cut(s) 136, 336, 349
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 3 cut(s) 288, 345, 346
BssMI GATC 3 cut(s) 130, 141, 341
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 134, 198, 225
BstDSI CCRYGG 1 cut(s) 288
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 3 cut(s) 133, 144, 344
BstMAI GTCTC 2 cut(s) 228, 387
BstMBI GATC 3 cut(s) 130, 141, 341
BstNI CCWGG 1 cut(s) 34
BstSCI CCNGG 3 cut(s) 32, 344, 345
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 141, 341
BstYI RGATCY 2 cut(s) 141, 341
Bsu15I ATCGAT 2 cut(s) 153, 418
BsuRI GGCC 1 cut(s) 317
BsuTUI ATCGAT 2 cut(s) 153, 418
BtgI CCRYGG 1 cut(s) 288
BtgZI GCGATG 2 cut(s) 219, 316
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 125, 296
Cfr9I CCCGGG 1 cut(s) 345
ClaI ATCGAT 2 cut(s) 153, 418
CseI GACGC 2 cut(s) 51, 346
Csp6I GTAC 1 cut(s) 213
CviAII CATG 1 cut(s) 289
CviJI RGCY 7 cut(s) 17, 37, 68, 95, 138, 284, 317
CviKI_1 RGCY 7 cut(s) 17, 37, 68, 95, 138, 284, 317
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 3 cut(s) 134, 198, 225
DpnI GATC 3 cut(s) 132, 143, 343
DpnII GATC 3 cut(s) 130, 141, 341
Eco130I CCWWGG 1 cut(s) 288
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 125, 296
Eco88I CYCGRG 1 cut(s) 345
EcoO109I RGGNCCY 2 cut(s) 125, 296
EcoRII CCWGG 1 cut(s) 32
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 1 cut(s) 292
FaiI YATR 1 cut(s) 290
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 96, 352, 355
Fsp4HI GCNGC 3 cut(s) 96, 352, 355
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 96, 352, 355
GsaI CCCAGC 1 cut(s) 308
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 317
HapII CCGG 1 cut(s) 346
HgaI GACGC 2 cut(s) 51, 346
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 2 cut(s) 192, 395
HpaII CCGG 1 cut(s) 346
Hpy188I TCNGA 3 cut(s) 178, 228, 400
Hpy188III TCNNGA 2 cut(s) 123, 392
Hpy99I CGWCG 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 62, 98, 254
HpyF3I CTNAG 3 cut(s) 134, 198, 225
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 1 cut(s) 183
KflI GGGWCCC 1 cut(s) 125
Kzo9I GATC 3 cut(s) 130, 141, 341
LpnPI CCDG 7 cut(s) 3, 19, 46, 152, 245, 290, 359
Lsp1109I GCAGC 1 cut(s) 82
MalI GATC 3 cut(s) 132, 143, 343
MboI GATC 3 cut(s) 130, 141, 341
MboII GAAGA 4 cut(s) 86, 111, 259, 275
MflI RGATCY 2 cut(s) 141, 341
MluCI AATT 2 cut(s) 78, 277
MluI ACGCGT 1 cut(s) 43
MlyI GAGTC 2 cut(s) 186, 389
MnlI CCTC 6 cut(s) 14, 17, 58, 155, 307, 395
MroXI GAANNNNTTC 1 cut(s) 78
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 3 cut(s) 34, 346, 347
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 45
NciI CCSGG 2 cut(s) 346, 347
NcoI CCATGG 1 cut(s) 288
NdeII GATC 3 cut(s) 130, 141, 341
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 4 cut(s) 126, 127, 298, 343
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 78
PkrI GCNGC 3 cut(s) 97, 353, 356
PleI GAGTC 2 cut(s) 186, 389
PpsI GAGTC 2 cut(s) 186, 389
PpuMI RGGWCCY 2 cut(s) 125, 296
Psp5II RGGWCCY 2 cut(s) 125, 296
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 4 cut(s) 126, 127, 298, 343
PspPI GGNCC 2 cut(s) 125, 296
PspPPI RGGWCCY 2 cut(s) 125, 296
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 2 cut(s) 141, 341
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SatI GCNGC 3 cut(s) 96, 352, 355
Sau3AI GATC 3 cut(s) 130, 141, 341
Sau96I GGNCC 2 cut(s) 125, 296
ScaI AGTACT 1 cut(s) 214
SchI GAGTC 2 cut(s) 186, 389
ScrFI CCNGG 3 cut(s) 34, 346, 347
SetI ASST 4 cut(s) 28, 35, 97, 151
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 2 cut(s) 125, 296
SmaI CCCGGG 1 cut(s) 347
Sse9I AATT 2 cut(s) 78, 277
SsiI CCGC 2 cut(s) 351, 354
StyD4I CCNGG 3 cut(s) 32, 344, 345
StyI CCWWGG 1 cut(s) 288
TaqI TCGA 6 cut(s) 7, 153, 190, 360, 391, 418
TasI AATT 2 cut(s) 78, 277
TatI WGTACW 1 cut(s) 212
TauI GCSGC 2 cut(s) 354, 357
TscAI CASTG 1 cut(s) 64
TseI GCWGC 1 cut(s) 95
TspMI CCCGGG 1 cut(s) 345
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 125, 296
XapI RAATTY 2 cut(s) 78, 277
XmaI CCCGGG 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.