Rw5G029920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004885
Physical Location & Seq
Forward (+)
46784756 .. 46785666
911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029920.1

Sequence Viewer

Length: 411 bp
ATGCTTCGAGGCGTGGCTGGAGGAGGTGCAACCTGGCCTGGACGCGTGGGGAAACGCCTTGCAGAGGCTTTCGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGTAAAACTTCTTCGGGACCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGTAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGCTGGGCAAAGAGGCTGATAACAACAACATCGCCGTAGGATCCCGGGGCGGCGTTGTCGATAATCTTTTTGTCGGCAAATCCCTCAATCGAGACTCCGATATCGTCTTTGATTATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

6.34

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 45
AciI CCGC 1 cut(s) 339
AclWI GGATC 3 cut(s) 136, 324, 337
AcsI RAATTY 2 cut(s) 78, 277
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 32, 37
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 228, 375
AlwI GGATC 3 cut(s) 136, 324, 337
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 78, 277
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 2 cut(s) 334, 335
AsuII TTCGAA 1 cut(s) 72
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 1 cut(s) 125
BamHI GGATCC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
BbvI GCAGC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 308
BciT130I CCWGG 2 cut(s) 34, 39
BcnI CCSGG 2 cut(s) 334, 335
BcoDI GTCTC 2 cut(s) 228, 375
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 2 cut(s) 96, 340
BlsI GCNGC 2 cut(s) 97, 341
BmcAI AGTACT 1 cut(s) 214
Bme1390I CCNGG 4 cut(s) 34, 39, 334, 335
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 3 cut(s) 126, 127, 331
BmrFI CCNGG 4 cut(s) 34, 39, 334, 335
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 334, 335
Bsa29I ATCGAT 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 333, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 34, 39
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 333, 334
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 148, 189, 239
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 2 cut(s) 228, 375
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 72
Bsp143I GATC 3 cut(s) 130, 141, 329
BspACI CCGC 1 cut(s) 339
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 3 cut(s) 147, 190, 238
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 126, 127, 331
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 3 cut(s) 136, 324, 337
BspT104I TTCGAA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 333, 334
BssMI GATC 3 cut(s) 130, 141, 329
Bst2UI CCWGG 2 cut(s) 34, 39
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 134, 198, 225
BstENI CCTNNNNNAGG 1 cut(s) 62
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 3 cut(s) 133, 144, 332
BstMAI GTCTC 2 cut(s) 228, 375
BstMBI GATC 3 cut(s) 130, 141, 329
BstNI CCWGG 2 cut(s) 34, 39
BstSCI CCNGG 4 cut(s) 32, 37, 332, 333
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 141, 329
BstYI RGATCY 2 cut(s) 141, 329
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 1 cut(s) 37
BsuTUI ATCGAT 1 cut(s) 153
BtgZI GCGATG 2 cut(s) 219, 304
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 125
Cfr9I CCCGGG 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 213
CviAII CATG 1 cut(s) 289
CviJI RGCY 7 cut(s) 17, 37, 68, 95, 138, 284, 305
CviKI_1 RGCY 7 cut(s) 17, 37, 68, 95, 138, 284, 305
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 3 cut(s) 134, 198, 225
DpnI GATC 3 cut(s) 132, 143, 331
DpnII GATC 3 cut(s) 130, 141, 329
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 32, 37
EcoRV GATATC 1 cut(s) 391
FaeI CATG 1 cut(s) 292
FaiI YATR 1 cut(s) 290
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 96, 340
Fsp4HI GCNGC 2 cut(s) 96, 340
GlaI GCGC 1 cut(s) 184
GluI GCNGC 2 cut(s) 96, 340
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 37
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 2 cut(s) 192, 383
HpaII CCGG 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 178, 199, 228, 388
Hpy188III TCNNGA 2 cut(s) 123, 380
HpyCH4V TGCA 3 cut(s) 29, 62, 98
HpyF3I CTNAG 3 cut(s) 134, 198, 225
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 1 cut(s) 183
KflI GGGWCCC 1 cut(s) 125
Kzo9I GATC 3 cut(s) 130, 141, 329
LpnPI CCDG 9 cut(s) 3, 19, 24, 46, 51, 152, 245, 278, 347
Lsp1109I GCAGC 1 cut(s) 82
MalI GATC 3 cut(s) 132, 143, 331
MboI GATC 3 cut(s) 130, 141, 329
MboII GAAGA 4 cut(s) 86, 111, 259, 275
MflI RGATCY 2 cut(s) 141, 329
MluCI AATT 2 cut(s) 78, 277
MluI ACGCGT 1 cut(s) 43
MlyI GAGTC 2 cut(s) 186, 377
MnlI CCTC 6 cut(s) 14, 17, 58, 155, 295, 383
MroXI GAANNNNTTC 1 cut(s) 78
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 4 cut(s) 34, 39, 334, 335
MvaI CCWGG 2 cut(s) 34, 39
MvnI CGCG 1 cut(s) 45
NciI CCSGG 2 cut(s) 334, 335
NdeII GATC 3 cut(s) 130, 141, 329
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 3 cut(s) 126, 127, 331
NspV TTCGAA 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 78
PkrI GCNGC 2 cut(s) 97, 341
PleI GAGTC 2 cut(s) 186, 377
PpsI GAGTC 2 cut(s) 186, 377
PpuMI RGGWCCY 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 32, 37
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 32, 37
PspN4I GGNNCC 3 cut(s) 126, 127, 331
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 2 cut(s) 141, 329
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SatI GCNGC 2 cut(s) 96, 340
Sau3AI GATC 3 cut(s) 130, 141, 329
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 214
SchI GAGTC 2 cut(s) 186, 377
ScrFI CCNGG 4 cut(s) 34, 39, 334, 335
SetI ASST 4 cut(s) 28, 35, 97, 151
SfcI CTRYAG 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 72
SinI GGWCC 1 cut(s) 125
SmaI CCCGGG 1 cut(s) 335
Sse9I AATT 2 cut(s) 78, 277
SsiI CCGC 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 32, 37, 332, 333
TaqI TCGA 6 cut(s) 7, 72, 153, 190, 348, 379
TasI AATT 2 cut(s) 78, 277
TatI WGTACW 1 cut(s) 212
TauI GCSGC 1 cut(s) 342
TseI GCWGC 1 cut(s) 95
TspMI CCCGGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 78, 277
XmaI CCCGGG 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.