Rroxscaffold_2G00133360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004885
Physical Location & Seq
Forward (+)
70534605 .. 70538969
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133360.1

Sequence Viewer

Length: 276 bp
ATGGATTCCCGGCACGCATCTCTAGGCCGCCGAATGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGACCCGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCAATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGTAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGGCTGGGCAAAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.14

Weight (kDa)

9.8

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 28
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 2 cut(s) 45, 244
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 37, 261
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 103
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 2 cut(s) 45, 244
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 92
AsuC2I CCSGG 1 cut(s) 10
AvaII GGWCC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 49
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 2 cut(s) 28, 63
BlsI GCNGC 2 cut(s) 29, 64
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 93, 94
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 261
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 178
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 255
BseLI CCNNNNNNNGG 2 cut(s) 37, 261
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 3 cut(s) 115, 156, 206
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 260
BshFI GGCC 1 cut(s) 27
BshVI ATCGAT 1 cut(s) 120
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 37, 261
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 97, 108
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 1 cut(s) 28
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 3 cut(s) 114, 157, 205
BspDI ATCGAT 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 93, 94
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 178
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 97, 108
BssT1I CCWWGG 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 15, 60
BstDEI CTNAG 3 cut(s) 101, 165, 192
BstDSI CCRYGG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 2 cut(s) 100, 111
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 2 cut(s) 97, 108
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 15, 60
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 92
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 1 cut(s) 180
CviAII CATG 1 cut(s) 256
CviJI RGCY 6 cut(s) 27, 62, 105, 251, 260, 273
CviKI_1 RGCY 6 cut(s) 27, 62, 105, 251, 260, 273
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 3 cut(s) 101, 165, 192
DpnI GATC 2 cut(s) 99, 110
DpnII GATC 2 cut(s) 97, 108
Eco130I CCWWGG 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 1 cut(s) 259
FaiI YATR 1 cut(s) 257
FaqI GGGAC 1 cut(s) 105
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 28, 63
Fsp4HI GCNGC 2 cut(s) 28, 63
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 151
GluI GCNGC 2 cut(s) 28, 63
GsaI CCCAGC 1 cut(s) 264
HaeIII GGCC 1 cut(s) 27
HapII CCGG 1 cut(s) 10
HhaI GCGC 1 cut(s) 152
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 5, 159
HpaII CCGG 1 cut(s) 10
Hpy188I TCNGA 3 cut(s) 145, 166, 195
Hpy188III TCNNGA 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 3 cut(s) 101, 165, 192
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 150
KflI GGGWCCC 1 cut(s) 92
Kzo9I GATC 2 cut(s) 97, 108
LpnPI CCDG 4 cut(s) 23, 119, 212, 246
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 23
MalI GATC 2 cut(s) 99, 110
MboI GATC 2 cut(s) 97, 108
MboII GAAGA 4 cut(s) 53, 78, 226, 242
MflI RGATCY 1 cut(s) 108
MluCI AATT 2 cut(s) 45, 244
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 2 cut(s) 122, 263
MroXI GAANNNNTTC 1 cut(s) 45
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 255
NdeII GATC 2 cut(s) 97, 108
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 93, 94
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 2 cut(s) 29, 64
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 93, 94
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
SatI GCNGC 2 cut(s) 28, 63
Sau3AI GATC 2 cut(s) 97, 108
Sau96I GGNCC 1 cut(s) 92
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 10
SetI ASST 2 cut(s) 64, 118
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 92
Sse9I AATT 2 cut(s) 45, 244
SsiI CCGC 1 cut(s) 28
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 255
TaqI TCGA 2 cut(s) 120, 157
TasI AATT 2 cut(s) 45, 244
TatI WGTACW 1 cut(s) 179
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 2 cut(s) 45, 244
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.