Rroxscaffold_7G00190740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004885
Physical Location & Seq
Reverse (-)
31000074 .. 31003611
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190740.1

Sequence Viewer

Length: 702 bp
ATGCCGATGGTTTCTTTCGGCACTGGGAGCATGCAGGCTTCGGAGGCATTGTTCTTCACCATGCCAATTGCCAAGAGGGTCAGTTTGTATTTGCTTTTGCATCACGACTTAGGGGTTCCAAGTGCCCTACACGCGGAGATTGAAATTGATGAAATCTCTCTTGCAAAGGTAATGCTTCGAGGCGTGGCTGGAGGAGGTGTGACCTGGCCTGGACGCGTGGGGAAACGCCGTGCAGAGGCTTTGGAAGAAATTAGTCAAAAGCGAGCTACAGAAAGATTGAGCAAAACTTCTTCGGGACCCGATCTCAGCCAGATCCCACCTATGGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTAAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATCTGGCTTCCATGGTAGGACTCTTGTGTTTGTTGTGCTTGTTTGTTTCTTTTCCGAAAGTTTTCACTTCAGGAGGGTTTGGTTGTGGGCAATGGTCAGTTTGGTTGCCTAATTATATTGTCGACTTATACAGGCTGGGCAAAGAGCCCGATAACAACAACATCGCCGTAGGATCCCGGGGCGGCGGCGTCGATAATCTTTTTGTCGGCAAATCCCTCAATCGAGACTCCGATATCGTCTTTGATTATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

25.45

Weight (kDa)

7.71

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 570
AccII CGCG 2 cut(s) 134, 216
AciI CCGC 3 cut(s) 134, 630, 633
AclWI GGATC 3 cut(s) 307, 615, 628
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 3 cut(s) 133, 235, 322
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 1 cut(s) 143
AjnI CCWGG 2 cut(s) 203, 208
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AluBI AGCT 1 cut(s) 266
AluI AGCT 1 cut(s) 266
Alw26I GTCTC 2 cut(s) 399, 666
AlwI GGATC 3 cut(s) 307, 615, 628
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 2 cut(s) 625, 626
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 127
BamHI GGATCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 597
BceAI ACGGC 2 cut(s) 213, 599
BciT130I CCWGG 2 cut(s) 205, 210
BcnI CCSGG 2 cut(s) 625, 626
BcoDI GTCTC 2 cut(s) 399, 666
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 2 cut(s) 631, 634
BlsI GCNGC 2 cut(s) 632, 635
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 4 cut(s) 205, 210, 625, 626
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 117, 297, 298, 622
BmrFI CCNGG 4 cut(s) 205, 210, 625, 626
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 210
BpuMI CCSGG 2 cut(s) 625, 626
Bsa29I ATCGAT 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 459, 624, 625
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 183, 213
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 235, 322
Bse1I ACTGG 2 cut(s) 28, 435
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 2 cut(s) 205, 210
BseCI ATCGAT 1 cut(s) 697
BseDI CCNNGG 3 cut(s) 459, 624, 625
BseLI CCNNNNNNNGG 3 cut(s) 133, 235, 322
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 2 cut(s) 319, 410
BseNI ACTGG 2 cut(s) 28, 435
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 127
BseYI CCCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 252
Bsh1236I CGCG 2 cut(s) 134, 216
BshFI GGCC 1 cut(s) 208
BshVI ATCGAT 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 1 cut(s) 625
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 133, 235, 322
BsmAI GTCTC 2 cut(s) 399, 666
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 127, 597
Bsp143I GATC 3 cut(s) 301, 312, 620
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 3 cut(s) 134, 630, 633
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 2 cut(s) 318, 409
BspDI ATCGAT 1 cut(s) 697
BspFNI CGCG 2 cut(s) 134, 216
BspLI GGNNCC 4 cut(s) 117, 297, 298, 622
BspPI GGATC 3 cut(s) 307, 615, 628
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 2 cut(s) 28, 435
BssECI CCNNGG 3 cut(s) 459, 624, 625
BssMI GATC 3 cut(s) 301, 312, 620
BssNI GRCGYC 1 cut(s) 636
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 205, 210
BstACI GRCGYC 1 cut(s) 636
BstC8I GCNNGC 3 cut(s) 32, 36, 264
BstDEI CTNAG 4 cut(s) 109, 305, 369, 396
BstDSI CCRYGG 1 cut(s) 459
BstFNI CGCG 2 cut(s) 134, 216
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 3 cut(s) 304, 315, 623
BstMAI GTCTC 2 cut(s) 399, 666
BstMBI GATC 3 cut(s) 301, 312, 620
BstMWI GCNNNNNNNGC 3 cut(s) 27, 44, 131
BstNI CCWGG 2 cut(s) 205, 210
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 4 cut(s) 203, 208, 623, 624
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 127
BstUI CGCG 2 cut(s) 134, 216
BstX2I RGATCY 2 cut(s) 312, 620
BstYI RGATCY 2 cut(s) 312, 620
Bsu15I ATCGAT 1 cut(s) 697
BsuRI GGCC 1 cut(s) 208
BsuTUI ATCGAT 1 cut(s) 697
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 2 cut(s) 390, 595
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 3 cut(s) 32, 36, 264
CfoI GCGC 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 296
Cfr9I CCCGGG 1 cut(s) 624
ClaI ATCGAT 1 cut(s) 697
CseI GACGC 2 cut(s) 222, 625
Csp6I GTAC 1 cut(s) 384
CviAII CATG 3 cut(s) 31, 61, 460
CviJI RGCY 9 cut(s) 38, 188, 208, 239, 266, 309, 455, 583, 595
CviKI_1 RGCY 9 cut(s) 38, 188, 208, 239, 266, 309, 455, 583, 595
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 4 cut(s) 109, 305, 369, 396
DpnI GATC 3 cut(s) 303, 314, 622
DpnII GATC 3 cut(s) 301, 312, 620
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 203, 208
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 34, 64, 463
FaiI YATR 6 cut(s) 32, 62, 323, 461, 564, 577
FaqI GGGAC 1 cut(s) 309
FatI CATG 3 cut(s) 30, 60, 459
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 2 cut(s) 631, 634
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 2 cut(s) 631, 634
GlaI GCGC 1 cut(s) 355
GluI GCNGC 2 cut(s) 631, 634
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 208
HapII CCGG 1 cut(s) 625
HgaI GACGC 2 cut(s) 222, 625
HhaI GCGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 3 cut(s) 34, 64, 463
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 3 cut(s) 363, 468, 674
HpaII CCGG 1 cut(s) 625
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 5 cut(s) 43, 349, 399, 504, 679
Hpy188III TCNNGA 4 cut(s) 104, 294, 519, 671
Hpy8I GTNNAC 1 cut(s) 571
Hpy99I CGWCG 1 cut(s) 641
HpyCH4V TGCA 5 cut(s) 34, 100, 164, 233, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 44, 131
HpyF3I CTNAG 4 cut(s) 109, 305, 369, 396
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 3 cut(s) 34, 64, 463
HspAI GCGC 1 cut(s) 354
KflI GGGWCCC 1 cut(s) 296
Kzo9I GATC 3 cut(s) 301, 312, 620
LmnI GCTCC 1 cut(s) 27
LweI GCATC 1 cut(s) 109
MaeIII GTNAC 1 cut(s) 199
MalI GATC 3 cut(s) 303, 314, 622
MboI GATC 3 cut(s) 301, 312, 620
MboII GAAGA 5 cut(s) 46, 257, 282, 430, 446
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 2 cut(s) 312, 620
MhlI GDGCHC 2 cut(s) 127, 597
MluCI AATT 4 cut(s) 66, 144, 249, 559
MluI ACGCGT 1 cut(s) 214
MlyI GAGTC 3 cut(s) 357, 462, 668
MnlI CCTC 9 cut(s) 37, 69, 173, 185, 188, 229, 326, 515, 674
MroXI GAANNNNTTC 1 cut(s) 509
MspI CCGG 1 cut(s) 625
MspR9I CCNGG 4 cut(s) 205, 210, 625, 626
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 2 cut(s) 205, 210
MvnI CGCG 2 cut(s) 134, 216
MwoI GCNNNNNNNGC 3 cut(s) 27, 44, 131
NciI CCSGG 2 cut(s) 625, 626
NcoI CCATGG 1 cut(s) 459
NdeII GATC 3 cut(s) 301, 312, 620
NlaIII CATG 3 cut(s) 34, 64, 463
NlaIV GGNNCC 4 cut(s) 117, 297, 298, 622
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 34
PaeI GCATGC 1 cut(s) 34
PctI GAATGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 509
PkrI GCNGC 2 cut(s) 632, 635
PleI GAGTC 3 cut(s) 357, 462, 668
PpsI GAGTC 3 cut(s) 357, 462, 668
PpuMI RGGWCCY 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 203, 208
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 2 cut(s) 203, 208
PspN4I GGNNCC 4 cut(s) 117, 297, 298, 622
PspPI GGNCC 1 cut(s) 296
PspPPI RGGWCCY 1 cut(s) 296
PsuI RGATCY 2 cut(s) 312, 620
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 569
SatI GCNGC 2 cut(s) 631, 634
Sau3AI GATC 3 cut(s) 301, 312, 620
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 385
SchI GAGTC 3 cut(s) 357, 462, 668
ScrFI CCNGG 4 cut(s) 205, 210, 625, 626
SduI GDGCHC 2 cut(s) 127, 597
SetI ASST 5 cut(s) 171, 199, 206, 268, 322
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 296
SmaI CCCGGG 1 cut(s) 626
SphI GCATGC 1 cut(s) 34
Sse9I AATT 4 cut(s) 66, 144, 249, 559
SsiI CCGC 3 cut(s) 134, 630, 633
StyD4I CCNGG 4 cut(s) 203, 208, 623, 624
StyI CCWWGG 1 cut(s) 459
TaqI TCGA 6 cut(s) 178, 361, 570, 639, 670, 697
TasI AATT 4 cut(s) 66, 144, 249, 559
TatI WGTACW 1 cut(s) 383
TauI GCSGC 2 cut(s) 633, 636
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 165
TspMI CCCGGG 1 cut(s) 624
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 296
XceI RCATGY 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 624
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 509
ZrmI AGTACT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.