RLG00000032727

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004885
Physical Location & Seq
Forward (+)
18351679 .. 18352716
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032727

Sequence Viewer

Length: 411 bp
ATGCTTCGAGGCGTGGCTGGAGGAGGTGCGACCTGGCCTGGACGCGTGGGGCGAAGGGAAGGCAAAGTGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGACCCGATCTCAGCCAGATCCTACCTATCAATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTGAGAGCGATGTTAGTACTTTAGTTTCTCAGATAATAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAACAAAAATTTGGCTTCCATGGCTGGGCAAAGAGGCCGATAACAACAACATCACCGTAAAATCCCGGGGCGGCGTCGTCGATAATCTTTTTGTCGGCAAATCCCTCAATCGAGACTCCGATATTGTCTTTGATTATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.04

Weight (kDa)

9.39

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 45
AciI CCGC 1 cut(s) 339
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 2 cut(s) 78, 276
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 32, 37
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 375
AlwI GGATC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 2 cut(s) 35, 303
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 78, 276
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 2 cut(s) 334, 335
AsuHPI GGTGA 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 82
BciT130I CCWGG 2 cut(s) 34, 39
BcnI CCSGG 2 cut(s) 334, 335
BcoDI GTCTC 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 2 cut(s) 96, 340
BlsI GCNGC 2 cut(s) 97, 341
BmcAI AGTACT 1 cut(s) 214
Bme1390I CCNGG 4 cut(s) 34, 39, 334, 335
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 126, 127
BmrFI CCNGG 4 cut(s) 34, 39, 334, 335
BpmI CTGGAG 1 cut(s) 39
BpuMI CCSGG 2 cut(s) 334, 335
Bsa29I ATCGAT 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 287, 333, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 34, 39
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 3 cut(s) 287, 333, 334
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 3 cut(s) 148, 189, 239
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 37, 305
BshVI ATCGAT 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 375
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 37, 305
BsoBI CYCGRG 1 cut(s) 333
Bsp143I GATC 2 cut(s) 130, 141
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 1 cut(s) 339
BspANI GGCC 2 cut(s) 37, 305
BspCNI CTCAG 3 cut(s) 147, 190, 238
BspDI ATCGAT 1 cut(s) 406
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 2 cut(s) 126, 127
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 3 cut(s) 287, 333, 334
BssMI GATC 2 cut(s) 130, 141
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 34, 39
Bst4CI ACNGT 1 cut(s) 325
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 134, 198, 225
BstDSI CCRYGG 1 cut(s) 287
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 2 cut(s) 133, 144
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 2 cut(s) 130, 141
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 2 cut(s) 34, 39
BstSCI CCNGG 4 cut(s) 32, 37, 332, 333
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
Bsu15I ATCGAT 1 cut(s) 406
BsuRI GGCC 2 cut(s) 37, 305
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 125
Cfr9I CCCGGG 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 2 cut(s) 51, 331
Csp6I GTAC 1 cut(s) 213
CviAII CATG 1 cut(s) 288
CviJI RGCY 7 cut(s) 17, 37, 95, 138, 283, 292, 305
CviKI_1 RGCY 7 cut(s) 17, 37, 95, 138, 283, 292, 305
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 3 cut(s) 134, 198, 225
DpnI GATC 2 cut(s) 132, 143
DpnII GATC 2 cut(s) 130, 141
Eco130I CCWWGG 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 32, 37
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 1 cut(s) 291
FaiI YATR 1 cut(s) 289
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 287
Fnu4HI GCNGC 2 cut(s) 96, 340
Fsp4HI GCNGC 2 cut(s) 96, 340
GlaI GCGC 1 cut(s) 184
GluI GCNGC 2 cut(s) 96, 340
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 37, 305
HapII CCGG 1 cut(s) 334
HgaI GACGC 2 cut(s) 51, 331
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 1 cut(s) 291
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 2 cut(s) 192, 383
HpaII CCGG 1 cut(s) 334
HphI GGTGA 1 cut(s) 313
Hpy188I TCNGA 4 cut(s) 178, 199, 228, 388
Hpy188III TCNNGA 2 cut(s) 123, 380
Hpy99I CGWCG 2 cut(s) 347, 350
HpyAV CCTTC 2 cut(s) 48, 53
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 2 cut(s) 98, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 3 cut(s) 134, 198, 225
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 1 cut(s) 291
HspAI GCGC 1 cut(s) 183
KflI GGGWCCC 1 cut(s) 125
Kzo9I GATC 2 cut(s) 130, 141
LpnPI CCDG 9 cut(s) 3, 19, 24, 46, 51, 152, 245, 278, 347
Lsp1109I GCAGC 1 cut(s) 82
MalI GATC 2 cut(s) 132, 143
MboI GATC 2 cut(s) 130, 141
MboII GAAGA 4 cut(s) 86, 111, 259, 275
MflI RGATCY 1 cut(s) 141
MluCI AATT 2 cut(s) 78, 276
MluI ACGCGT 1 cut(s) 43
MlyI GAGTC 2 cut(s) 186, 377
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 5 cut(s) 14, 17, 155, 295, 383
MroXI GAANNNNTTC 1 cut(s) 78
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 4 cut(s) 34, 39, 334, 335
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 2 cut(s) 34, 39
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 289
NciI CCSGG 2 cut(s) 334, 335
NcoI CCATGG 1 cut(s) 287
NdeII GATC 2 cut(s) 130, 141
NlaIII CATG 1 cut(s) 291
NlaIV GGNNCC 2 cut(s) 126, 127
PcsI WCGNNNNNNNCGW 2 cut(s) 49, 88
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 78
PkrI GCNGC 2 cut(s) 97, 341
PleI GAGTC 2 cut(s) 186, 377
PpsI GAGTC 2 cut(s) 186, 377
PpuMI RGGWCCY 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 32, 37
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 32, 37
PspN4I GGNNCC 2 cut(s) 126, 127
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 141
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SatI GCNGC 2 cut(s) 96, 340
Sau3AI GATC 2 cut(s) 130, 141
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 214
SchI GAGTC 2 cut(s) 186, 377
ScrFI CCNGG 4 cut(s) 34, 39, 334, 335
SetI ASST 4 cut(s) 28, 35, 97, 151
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 1 cut(s) 125
SmaI CCCGGG 1 cut(s) 335
Sse9I AATT 2 cut(s) 78, 276
SsiI CCGC 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 32, 37, 332, 333
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 325
TaqI TCGA 5 cut(s) 7, 190, 348, 379, 406
TasI AATT 2 cut(s) 78, 276
TatI WGTACW 1 cut(s) 212
TauI GCSGC 1 cut(s) 342
TseI GCWGC 1 cut(s) 95
TspMI CCCGGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 78, 276
XmaI CCCGGG 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.