RchiOBHm_Chr1g0356771

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004368
Physical Location & Seq
Forward (+)
49163871 .. 49164731
861 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58208

Sequence Viewer

Length: 624 bp
ATGCCTGTGAGAAATGTGGCGGCTTGGAATGCGTTAATTACTGGGTTTGTTGACTGTAGCAGAGAATGTTGTTGGAAAACAGCTGGTCCGGATGATATAACAGTTAATGGTTTGTTGCGCGGTTGCATAGAGTTGAAGGATGTAGAGATTGATAGACAATTGCATTGTTTTGCGGTGAAGTTAGGATTTGATTTGAGTTGCTTTGTGGGTAGCGCTCTTGTTGCCTGCTTAGGTGCAAGTAGTACTTGGGGATCTTGTGAACCCGGAAAGCATCATCATGGCACTGTTACTAGACAGTCGTTTAATAACATAGGCAATGCTCGCAAGGCTTTTGATGCGATGTCCATCAAGAATGTTGTTTCTTGGAACACTATAATTGTGGGATATGGACAGCATGGAAAAGGGATTCAAGCGGTAAAACTTCTGTGGGAAATGTTTAGAGAACATTTTTATCCTGATGAGTTTATTCTGGCAAGCATTGTTCGCACATGTGGCAATGTACCATCTGCTAATGAACTTATGGAAGTTCATGCTTACATCCAGGGACGTAGCCAGGATCGTAAGGAGGGTTGGGCTAATTTTAGCCTAACAAATATTTTGCGCAGAACTCTTTTTCTTTTTTGA

Protein Analysis

207

Amino Acids

23.07

Weight (kDa)

8.09

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 117 - 155 4.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 602
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 88
AciI CCGC 4 cut(s) 20, 120, 173, 413
AclWI GGATC 2 cut(s) 259, 564
AfaI GTAC 2 cut(s) 244, 501
AfeI AGCGCT 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 488
AgsI TTSAA 2 cut(s) 136, 410
AjnI CCWGG 2 cut(s) 540, 552
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
AlwI GGATC 2 cut(s) 259, 564
Aor13HI TCCGGA 1 cut(s) 88
Aor51HI AGCGCT 1 cut(s) 214
AspLEI GCGC 3 cut(s) 120, 215, 603
AspS9I GGNCC 1 cut(s) 86
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 1 cut(s) 86
BccI CCATC 2 cut(s) 353, 511
BciT130I CCWGG 2 cut(s) 542, 554
BcnI CCSGG 1 cut(s) 264
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 55
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 264, 542, 554
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmrFI CCNGG 3 cut(s) 264, 542, 554
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 2 cut(s) 280, 325
BmuI ACTGGG 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 541
BsaWI WCCGGW 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 2 cut(s) 322, 502
Bse8I GATNNNNATC 1 cut(s) 344
BseAI TCCGGA 1 cut(s) 88
BseBI CCWGG 2 cut(s) 542, 554
BseDI CCNNGG 1 cut(s) 541
BseGI GGATG 3 cut(s) 97, 145, 537
BseJI GATNNNNATC 1 cut(s) 344
BseMI GCAATG 2 cut(s) 322, 502
BseNI ACTGG 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 89, 264
BslFI GGGAC 1 cut(s) 558
BsmFI GGGAC 1 cut(s) 558
BsmI GAATGC 1 cut(s) 34
Bsp13I TCCGGA 1 cut(s) 88
Bsp143I GATC 2 cut(s) 251, 556
BspACI CCGC 4 cut(s) 20, 120, 173, 413
BspEI TCCGGA 1 cut(s) 88
BspFNI CGCG 1 cut(s) 120
BspPI GGATC 2 cut(s) 259, 564
BsrDI GCAATG 2 cut(s) 322, 502
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 541
BssMI GATC 2 cut(s) 251, 556
Bst2UI CCWGG 2 cut(s) 542, 554
Bst4CI ACNGT 4 cut(s) 56, 103, 286, 297
BstC8I GCNNGC 3 cut(s) 226, 322, 475
BstDEI CTNAG 1 cut(s) 229
BstF5I GGATG 3 cut(s) 97, 145, 537
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 3 cut(s) 120, 215, 603
BstKTI GATC 2 cut(s) 254, 559
BstMBI GATC 2 cut(s) 251, 556
BstMWI GCNNNNNNNGC 7 cut(s) 29, 221, 321, 326, 335, 483, 492
BstNI CCWGG 2 cut(s) 542, 554
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 3 cut(s) 262, 540, 552
BstSFI CTRYAG 1 cut(s) 55
BstUI CGCG 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BtgZI GCGATG 1 cut(s) 353
BtsCI GGATG 3 cut(s) 97, 145, 537
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 226, 322, 475
CfoI GCGC 3 cut(s) 120, 215, 603
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 243, 500
CviAII CATG 4 cut(s) 278, 395, 489, 530
CviJI RGCY 6 cut(s) 23, 83, 329, 552, 575, 585
CviKI_1 RGCY 6 cut(s) 23, 83, 329, 552, 575, 585
CviQI GTAC 2 cut(s) 243, 500
DdeI CTNAG 1 cut(s) 229
DpnI GATC 2 cut(s) 253, 558
DpnII GATC 2 cut(s) 251, 556
Eco47I GGWCC 1 cut(s) 86
Eco47III AGCGCT 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 540, 552
FaeI CATG 4 cut(s) 281, 398, 492, 533
FalI AAGNNNNNCTT 2 cut(s) 229, 261
FaqI GGGAC 1 cut(s) 558
FatI CATG 4 cut(s) 277, 394, 488, 529
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 3 cut(s) 104, 152, 524
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 1 cut(s) 291
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 3 cut(s) 119, 214, 602
GluI GCNGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 216
HapII CCGG 2 cut(s) 89, 264
HhaI GCGC 3 cut(s) 120, 215, 603
Hin1II CATG 4 cut(s) 281, 398, 492, 533
Hin6I GCGC 3 cut(s) 118, 213, 601
HinP1I GCGC 3 cut(s) 118, 213, 601
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 1 cut(s) 406
HpaII CCGG 2 cut(s) 89, 264
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 2 cut(s) 52, 260
Hpy188III TCNNGA 3 cut(s) 89, 349, 455
Hpy8I GTNNAC 2 cut(s) 52, 260
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 4 cut(s) 56, 103, 286, 297
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 3 cut(s) 126, 163, 236
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 221, 321, 326, 335, 483, 492
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 4 cut(s) 281, 398, 492, 533
HspAI GCGC 3 cut(s) 118, 213, 601
Kpn2I TCCGGA 1 cut(s) 88
Kzo9I GATC 2 cut(s) 251, 556
LweI GCATC 2 cut(s) 280, 325
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 1 cut(s) 286
MalI GATC 2 cut(s) 253, 558
MboI GATC 2 cut(s) 251, 556
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 251
MluCI AATT 4 cut(s) 36, 158, 375, 577
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 1 cut(s) 559
MroI TCCGGA 1 cut(s) 88
MseI TTAA 3 cut(s) 35, 105, 303
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 89, 264
MspR9I CCNGG 3 cut(s) 264, 542, 554
MunI CAATTG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 2 cut(s) 542, 554
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 7 cut(s) 29, 221, 321, 326, 335, 483, 492
NciI CCSGG 1 cut(s) 264
NdeII GATC 2 cut(s) 251, 556
NlaIII CATG 4 cut(s) 281, 398, 492, 533
NsbI TGCGCA 1 cut(s) 602
NspI RCATGY 1 cut(s) 492
PciI ACATGT 1 cut(s) 488
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 1 cut(s) 22
PscI ACATGT 1 cut(s) 488
Psp6I CCWGG 2 cut(s) 540, 552
PspGI CCWGG 2 cut(s) 540, 552
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 2 cut(s) 244, 501
RsaNI GTAC 2 cut(s) 243, 500
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 3 cut(s) 35, 105, 303
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 2 cut(s) 251, 556
Sau96I GGNCC 1 cut(s) 86
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 264, 542, 554
SetI ASST 3 cut(s) 85, 235, 550
SfaNI GCATC 2 cut(s) 280, 325
SfcI CTRYAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 4 cut(s) 36, 158, 375, 577
SsiI CCGC 4 cut(s) 20, 120, 173, 413
SspI AATATT 1 cut(s) 595
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 262, 540, 552
TaaI ACNGT 4 cut(s) 56, 103, 286, 297
TaiI ACGT 1 cut(s) 550
TasI AATT 4 cut(s) 36, 158, 375, 577
TatI WGTACW 1 cut(s) 242
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 3 cut(s) 35, 105, 303
Tru9I TTAA 3 cut(s) 35, 105, 303
TscAI CASTG 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 518, 528
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 86
XceI RCATGY 1 cut(s) 492
XspI CTAG 1 cut(s) 291
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.